LOCUS       pAc5.1/V5-His_A_CAHS8_DM_for_GA_GS_AcGFP        6789 bp    DNA     circular SYN 22-SEP-2022
DEFINITION  pAc5.1/V5-His A.
ACCESSION   urn.local...25-fc36wn1
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="pAc5.1/V5-His A"
                     /mol_type="other DNA"
     CDS             726..1454
                     /translation="MMPDHRLISASFAERSACAVVVFWNSFNFRTVRFDFSFLAYSKS
                     KVKPKKRDGNTKCGKTVVEGKGCGAAEGRVGRGVAGSVAGRDVTDVGATPLTMCACVY
                     YSCLATRRLFFSFYLALSSAISYACSTHRMLPCPLCVILARNRQLFKQRLVNENAKIN
                     KSTIWLLIAMCVQPTIATKATTQWLSLFHFLFASACEQDGTTGKRNYADKEQTKFWPK
                     NIKAPDTNAFAITIAIFNIVYEAV*"
                     /label="ORF frame 3"
     CDS             complement(1940..2416)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="ORF frame 2"
     promoter        2510..2571
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="promotor"
     misc_feature    2525..2544
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="AC5_primer"
     misc_feature    2608..4044
                     /Original_Bases="CTGCAGATATCCAGCACAGTGGC"
                     /label="CTGCAGATATCCAGCACAGTGGC"
                     /note="Geneious type: Editing History Replacement"
     motif           2608..2613
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="kozak_DM"
     CDS             2614..>3279
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS8_DM_for_GeneArt"
     primer          complement(3258..3299)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS8_DM_iF"
     linker          3280..3324
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="pBS Coupler segment"
     primer          3280..3304
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="N_V_iR"
     CDS             3325..4044
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="AcGFP"
     primer          complement(3332..3353)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="seq-AcGFP1-N1-Rev"
     primer          complement(4021..4044)
                     /created_by="saibou"
                     /label="GFP_rev"
     misc_feature    complement(4165..4182)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="BGH_rev_primer"
     terminator      complement(4225..4344)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="SV40_PA_terminator"
     misc_feature    complement(4239..4258)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="EBV_rev_primer"
     CDS             complement(4372..5001)
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAGKSRERSLKLSLMVVIYL
                     PGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQRVGRHAGDNGLLLAETFGGG
                     TSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCRHLSYELHDKEDSHKCGDD
                     SHAPRPPEGADWVEGSQGHRSTDHNQPYHICRGFTCFKKPPTPPPEPET*"
                     /label="ORF frame 1"
     CDS             4450..5052
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="ORF frame 3"
     rep_origin      complement(4956..5575)
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="pBR322_origin"
     gene            complement(5730..6590)
                     /gene="Ampicillin"
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="Ampicillin"
     CDS             complement(5730..6590)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 2"
     promoter        complement(6632..6660)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaattgcc accatgagtc agcgcgccag cgaacatagc
     2641 agcagcagtc atcagagcag cggccagagt ggacagagca gccagcatgt gcaagagcag
     2701 aacaccagct acacccacac cgatgtgcgc accaacgtgc ccaatgtgcc accaccagtg
     2761 atcagtacaa gtgccgccgg actgagcgag cagatggttg gacaaggatt cacagccagc
     2821 gccgctcgtg ttagcggaca aagtactgag gcccacgtgc agatgacccc agagatggaa
     2881 gccgaggctc gcaaggatcg cgaacgctat gaacgcgagc tggaagccat caaccagcgc
     2941 caccagaagg atatcgaggg caagacagag gcctaccgca agcaagccga agctgaagcc
     3001 gaacgcctgc gccgtgaact ggaaaagcag caccagcgcg acgtcgagtt tcgcaagagt
     3061 ctggtgcagg gcaccatcga caaccagaag aagcaggtcg agttggaggc catgatggcc
     3121 aagcgcgaac tggatcgtga agccaagctg gcccaagatg ccctggaaaa gtccaagatg
     3181 gccacggatg cccaagtgac ctttgagagc gccgtgggcc ataccattag cggcagtaca
     3241 acagtgtccc agagcgagaa ggtgtcccag tccaagaagg gtggaggcgg ttcaggcgga
     3301 ggtggctctg gcggtggcgg atcgatggtg agcaagggcg ccgagctgtt caccggcatc
     3361 gtgcccatcc tgatcgagct gaatggcgat gtgaatggcc acaagttcag cgtgagcggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcctg tgccctggcc caccctggtg accaccctga gctacggcgt gcagtgcttc
     3541 tcacgctacc ccgatcacat gaagcagcac gacttcttca agagcgccat gcctgagggc
     3601 tacatccagg agcgcaccat cttcttcgag gatgacggca actacaagtc gcgcgccgag
     3661 gtgaagttcg agggcgatac cctggtgaat cgcatcgagc tgaccggcac cgatttcaag
     3721 gaggatggca acatcctggg caataagatg gagtacaact acaacgccca caatgtgtac
     3781 atcatgaccg acaaggccaa gaatggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggatggca gcgtgcagct ggccgaccac taccagcaga atacccccat cggcgatggc
     3901 cctgtgctgc tgcccgataa ccactacctg tccacccaga gcgccctgtc caaggacccc
     3961 aacgagaagc gcgatcacat gatctacttc ggcttcgtga ccgccgccgc catcacccac
     4021 ggcatggatg agctgtacaa gtgaggccgc tcgagtctag agggcccttc gaaggtaagc
     4081 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
     4141 attgagttta aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat
     4201 cagcctcgat cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     4261 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4321 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4381 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4441 tggctgatta tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     4501 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     4561 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     4621 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     4681 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     4741 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     4801 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     4861 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     4921 accagccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4981 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5041 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5101 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5161 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5221 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5281 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5341 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5401 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5461 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5521 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5581 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5641 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     6061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     6361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6661 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6721 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6781 ccctttcgt
//
