LOCUS       pAc5.1/V5-His_A_CAHS12_DM_for_GA_GS_AcGFP        7014 bp    DNA     circular SYN 22-SEP-2022
DEFINITION  pAc5.1/V5-His A.
ACCESSION   urn.local...2h-fc3735y
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="pAc5.1/V5-His A"
                     /mol_type="other DNA"
     CDS             726..1454
                     /translation="MMPDHRLISASFAERSACAVVVFWNSFNFRTVRFDFSFLAYSKS
                     KVKPKKRDGNTKCGKTVVEGKGCGAAEGRVGRGVAGSVAGRDVTDVGATPLTMCACVY
                     YSCLATRRLFFSFYLALSSAISYACSTHRMLPCPLCVILARNRQLFKQRLVNENAKIN
                     KSTIWLLIAMCVQPTIATKATTQWLSLFHFLFASACEQDGTTGKRNYADKEQTKFWPK
                     NIKAPDTNAFAITIAIFNIVYEAV*"
                     /label="ORF frame 3"
     CDS             complement(1940..2416)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="ORF frame 2"
     promoter        2510..2571
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="promotor"
     misc_feature    2525..2544
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="AC5_primer"
     misc_feature    2608..4269
                     /Original_Bases="CTGCAGATATCCAGCACAGTGGC"
                     /label="CTGCAGATATCCAGCACAGTGGC"
                     /note="Geneious type: Editing History Replacement"
     motif           2608..2613
                     /created_by="saibou"
                     /label="kozak_DM"
     CDS             2614..>3504
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS12_DM_for_GeneArt"
     primer          complement(3485..3524)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS12_DM_iF"
     linker          3505..3549
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="pBS Coupler segment"
     primer          3505..3529
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="N_V_iR"
     CDS             3550..4269
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="AcGFP"
     primer          complement(4246..4269)
                     /created_by="saibou"
                     /label="GFP_rev"
     misc_feature    complement(4390..4407)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="BGH_rev_primer"
     terminator      complement(4450..4569)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="SV40_PA_terminator"
     misc_feature    complement(4464..4483)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="EBV_rev_primer"
     CDS             complement(4597..5226)
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAGKSRERSLKLSLMVVIYL
                     PGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQRVGRHAGDNGLLLAETFGGG
                     TSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCRHLSYELHDKEDSHKCGDD
                     SHAPRPPEGADWVEGSQGHRSTDHNQPYHICRGFTCFKKPPTPPPEPET*"
                     /label="ORF frame 1"
     CDS             4675..5277
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="ORF frame 3"
     rep_origin      complement(5181..5800)
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="pBR322_origin"
     gene            complement(5955..6815)
                     /gene="Ampicillin"
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="Ampicillin"
     CDS             complement(5955..6815)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 2"
     promoter        complement(6857..6885)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaattgcc accatgagtc acacccacga gcaaaagttc
     2641 gagcgcgtgg aagaacgcaa ggtggacgat aagaaaggcc tgcaagaagt gcgcgtcggc
     2701 gttgacacag gacatggcga tcccgccttg aacttcaccc caaccgatgc cacactcgtg
     2761 cgcacaggtg gtgttggagg aacaacagcc agcagccaca gctcccatat gagcagtagt
     2821 acaggcggag ccgtgacagg cgctagccag tatagtagca ccatgcacca agagggcggc
     2881 cacatgacaa cagaggccag caagaacacc agctacaccc acaccgaagt gcgtgcccca
     2941 gtgatcgata cagccccgcc aattattagc accggcgcca gtggaatggc cgagcagata
     3001 gttggacagg gctttacagc cagcgccgct cgcattacag gatccagcgc cgatgtgaac
     3061 atcgtggaaa cagccgaggc tcgcgagaag cgcatgcgtg atgaagagaa gtacgcccgc
     3121 gagcaagagg ccatcaatcg ccacgccgat aaggacctgg aaaagaaaac cgaggcctac
     3181 cgcaaagagg ccgagcacga ggccgaaaag atccgcaagg ctctggaaaa gcagcacgag
     3241 cgcgatatcg agttccgcaa agaagtcgtg ggcagcacca tcgagaagca gaaggccgag
     3301 attgagctgg aagccaagcg tgccaaggcc gctctggaac atgaacgaca gctggccaac
     3361 gatgccctgg aacgcagcaa gatgcacacc gatgtgcaag tgaccatgga taccgccgct
     3421 ggacatacag tgtccggcgg aacaacaatg agcagcagcg agcagcatag cagctcccac
     3481 agtagtagtc gcaccggcgg actgggtgga ggcggttcag gcggaggtgg ctctggcggt
     3541 ggcggatcga tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
     3601 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
     3661 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
     3721 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
     3781 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
     3841 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc
     3901 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc
     3961 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag
     4021 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg
     4081 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     4141 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     4201 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     4261 tacaagtgag gccgctcgag tctagagggc ccttcgaagg taagcctatc cctaaccctc
     4321 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     4381 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     4441 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     4501 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     4561 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     4621 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     4681 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     4741 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     4801 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     4861 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     4921 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     4981 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     5041 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     5101 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag
     5161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     5401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5701 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5761 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5821 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5881 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5941 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6001 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6061 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6121 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6181 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6241 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     6301 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6361 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6421 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6481 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6541 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     6601 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6661 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6721 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6781 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6841 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6901 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     6961 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
