LOCUS       lentiGuideFB-Puro-A    10676 bp ds-DNA     circular     22-SEP-2022
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     LTR             1082..1270
                     /label="5' LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1321..1458
                     /label="Psi"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1425..1789
                     /label="gag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1935..2176
                     /label="RRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2510..2536
                     /label="HW_088"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2694..2811
                     /label="CPPT; central polypurine tract"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2862..3106
                     /label="hU6 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3107..4991
                     /label="Filler"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4992..5103
                     /label="sgRNA scaffold"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5058..5079
                     /label="10x Feature BC 1.1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(5110..5160)
                     /label="HW_204"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        5137..5392
                     /label="EFS-NS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5407..6006
                     /label="Puro"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_binding    6022..6610
                     /label="WPRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     LTR             6681..6916
                     /label="3' LTR SIN"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      9365..9590
                     /label="ColE1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
       61 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      241 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
      301 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
      361 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      421 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      481 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      541 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      601 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      661 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      721 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      781 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      841 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      901 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      961 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1021 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     1081 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     1141 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1201 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1261 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1321 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1381 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1441 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1501 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1561 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1621 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1681 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1741 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1801 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1861 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1921 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1981 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     2041 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     2101 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     2161 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     2221 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     2281 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2341 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2401 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2461 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2521 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2581 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2641 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2701 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2761 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2821 tacagggaca gcagagatcc agtttggtta attaaggtac cgagggccta tttcccatga
     2881 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     2941 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     3001 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     3061 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacggtt
     3121 gtaaatgagc acacaaaata cacatgctaa aatattatat tctatgacct ttataaaatc
     3181 aaccaaaatc ttctttttaa taactttagt atcaataatt agaattttta tgttcctttt
     3241 tgcaaacttt taataaaaat gagcaaaata aaaaaacgct agttttagta actcgcgttg
     3301 ttttcttcac ctttaataat agctactcca ccacttgttc ctaagcggtc agctcctgct
     3361 tcaatcattt tttgagcatc ttcaaatgtt ctaactccac cagctgcttt aactaaagca
     3421 ttgtctttaa caactgactt cattagttta acatcttcaa atgttgcacc tgattttgaa
     3481 aatcctgttg atgttttaac aaattctaat ccagcttcaa cagctatttc acaagctttc
     3541 atgatttctt cttttgttaa taaacaattt tccataatac atttaacaac atgtgatcca
     3601 gctgcttttt ttacagcttt catgtcttct aaaactaatt cataattttt gtcttttaat
     3661 gcaccaatat ttaataccat atcaatttct gttgcaccat ctttaattgc ttcagaaact
     3721 tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa aacctacaac atttgttatt
     3781 cctacatttg tgccttttaa taattcttta caatagcttg ttcaatatga attaacacaa
     3841 actgttgcaa aatcaaattc aattgcttca tcacataatt gtttaatttc agctttcgta
     3901 gcatcttgtt ttaataatgt gtgatctata tatttgttta gtttcatttt ttctcctata
     3961 tattcatttt taattttaat tctttaataa tttcgtctac tttaacttta gcgttttgaa
     4021 cagattcacc aacacctata aaataaattt ttagtttagg ttcagttcca cttgggcgaa
     4081 cagcaaatca tgacttatct tctaaataaa attttagtaa gtcttgtcct ggcatattat
     4141 acattccatc gatgtagtct tcaacattaa caactttaag tccagcaatt tgagttaagg
     4201 gtgttgctct caatgatttc attaatggtt caatttttaa tttcttttct tctggtttaa
     4261 aattcaagtt taaagtgaaa gtgtaatatg cacccatttc tttaaataaa tcttctaaat
     4321 agtctactaa tgttttattt tgttttttat aaaatcaagc agcctctgct attaatatag
     4381 aagcttgtat tccatcttta tctctagctg agtcatcaat tacatatcca taactttctt
     4441 cataagcaaa aacaaaattt aatccgttat cttcttcttt agcaatttct ctacccattc
     4501 atttaaatcc agttaaagtt tttacaatat taactccata tttttcatga gcgattctat
     4561 cacccaaatc acttgttaca aaacttgaat atagagccgg attttttgga atgctattta
     4621 agcgttttag atttgataat tttcaatcaa ttaaaattgg tcctgtttga tttccatcta
     4681 atcttacaaa atgaccatca tgttttattg ccattccaaa tctgtcagca tctgggtcat
     4741 tcataataat aatatctgca tcatgtttaa taccatattc aagcggtatt tttcatgcag
     4801 gatcaaattc tggatttgga tttacaacat ttttaaatgt ttcatcttca aatgcatgct
     4861 ctttaacctc aataacgtta tatcctgatt cacgtaatat ttttggggta aatttagttc
     4921 ctgttccatt aactgcgcta aaaataattt ttaaatcttt tttagcttct tgctcttttt
     4981 tgtacgtctc tGTTTAAGAG CTAAGCTGGA AACAGCATAG CAAGTTTAAA TAAGGCTAGT
     5041 CCGTTATCAA CTTggccGCT TTAAGGCCGG TCCTAGCAAg gccAAGTGGC ACCGAGTCGG
     5101 TGCtttttta agcttggcgG GATCCGGTAC GCTAGCTAGG TCTTGAAAGG AGTGggaatt
     5161 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     5221 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5281 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5341 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc
     5401 tagagcATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG
     5461 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT
     5521 CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG
     5581 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG
     5641 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG
     5701 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC
     5761 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGAGTCTCGC CCGACCACCA GGGCAAGGGT
     5821 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC
     5881 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC
     5941 ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT
     6001 GCCTGAACGC GTTAAGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT
     6061 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG
     6121 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG
     6181 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG
     6241 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG
     6301 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC
     6361 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA
     6421 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC
     6481 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT
     6541 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC
     6601 GCCTCCCCGC GTCGACTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT
     6661 TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATCTGC
     6721 TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT
     6781 AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT
     6841 GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT
     6901 GGAAAATCTC TAGCAGggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     6961 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7021 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7081 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7141 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     7201 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7621 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     7681 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7921 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7981 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     8041 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     8101 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     8161 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     8221 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     8281 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     8341 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     8401 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     8461 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     8521 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8581 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8641 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8701 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8761 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8821 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8881 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8941 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9001 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9061 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9121 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9181 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9241 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9301 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9361 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9421 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9481 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9541 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9601 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9661 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9721 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9781 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9841 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9901 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9961 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10021 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10081 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10141 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10201 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10261 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10321 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10381 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10441 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10501 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10561 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10621 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactc
//