LOCUS       TL021_SEP-GluA1_RH_linkers_lenti_CMV       12060 bp ds-DNA     circular     23-JUL-2020
DEFINITION  .
ACCESSION   -
KEYWORDS    -.
SOURCE      .
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    254..817
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMV promoter"
     misc_feature    772..792
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMV_fwd_primer"
     LTR             835..1015
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="5'LTR"
     misc_feature    956..1074
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Primer binding site (PBS)"
     misc_feature    1075..1187
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Psi packaging signal"
     misc_feature    1125..1126
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="splice donor (SD)"
     misc_feature    1620..1974
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Rev Response Element (RRE)"
     misc_feature    2284..2293
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="splice acceptor? (SA)"
     misc_feature    2432..2464
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="central polypurine tract (cPPT)"
     misc_feature    2594..2601
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="PacI"
     promoter        2602..3199
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMV prmoter"
     misc_feature    3200..3205
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI"
     ORF             3209..6727
                     /created_by="xiuyuanli"
                     /label="SP-linker-SEP-linker-GluA1"
     CDS             3209..3268
                     /created_by="user"
                     /modified_by="xiuyuanli"
                     /label="Signaling peptide"
     misc_feature    3236..3241
                     /Recognition_pattern="A^CCGGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiAI, AsiGI, BshTI, CsiAI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     CDS             3311..4021
                     /created_by="user"
                     /modified_by="xiuyuanli"
                     /label="Superecliptic pHluorin"
     misc_feature    complement(3356..3369)
                     /Recognition_pattern="TCANNNNNNNRTTC"
                     /annotation_group="TCANNNNNNNRTTC"
                     /label="EcoDXXI"
                     /note="Geneious type: restriction site"
     misc_feature    3575..3587
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /Methylation="CpG:-"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3656..3666)
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /Methylation="Dcm:cut, CpG:variable, EcoBI:blocked"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    3749..3757
                     /Recognition_pattern="CACNNN^GTG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACNNNGTG"
                     /label="AdeI, BstIZ316I, DraIII"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3841..3855)
                     /Recognition_pattern="TGANNNNNNNNTGCT"
                     /annotation_group="TGANNNNNNNNTGCT"
                     /label="Eco1158I, Eco1265I, Eco1441I, Eco1443I, Eco1444I,
                     Eco1447I, EcoBI"
                     /note="Geneious type: restriction site"
     misc_feature    3867..3872
                     /Recognition_pattern="C^AATTG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    4018..4029
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /Methylation="CpG:cut"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     CDS             4067..6727
                     /created_by="user"
                     /modified_by="xiuyuanli"
                     /label="GluA1"
     misc_feature    4178..4183
                     /Recognition_pattern="AT^CGAT"
                     /Methylation="Dam:blocked, CpG:blocked, EcoBI:variable"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     misc_feature    4178..4183
                     /Recognition_pattern="ATCGAT"
                     /annotation_group="ATCGAT"
                     /label="Apu16I, Asp123I, Asp130I, Asp14I, Asp37I,
                     Asp707I,Asp86I, BazI, Bco79I, BfrAI, Bli576I, Bli585I,
                     BpsII,Bsh108AI, Bsp125I, Bsp126I, Bsp127I, Bsp145I,
                     Bsp2I,Bsp4I, Bsp84I, BsrCI, BstLVI, Bth1202I, Bth9415I,
                     BtuI,Csp4I, SonI, Uba1096I, Uba1100I, Uba1133I,
                     Uba1137I,Uba1138I, Uba1144I, Uba1145I, Uba1161I,
                     Uba1168I,Uba1195I, Uba1196I, Uba1197I, Uba1198I,
                     Uba1199I,Uba1200I, Uba1233I, Uba1238I, Uba1246I,
                     Uba1257I,Uba1275I, Uba1286I, Uba1295I, Uba1315I,
                     Uba1342I,Uba1366II, Uba1379I, Uba1380I, Uba1394I,
                     Uba1412I,Uba1416I, Uba1427I, Uba1430I, Uba1451I, Uba1453I,
                     Uba22I,Uba24I, Uba30I, Uba34I, Uba43I, UbaN18I, UbaN22I"
                     /note="Geneious type: restriction site"
     misc_feature    4178..4183
                     /Recognition_pattern="AT^CGAT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ATCGAT"
                     /label="AagI, BanIII, BavCI, BbvAII, Bci29I, BciBI, BcmI,
                     BdiI, Bli41I, Bli86I, BliAI, BliRI, Bsa29I, BscI, BseCI,
                     BshVI, BsiXI, Bsp106I, BspDI, BspJII, BspOVII, BspXI,
                     BspZEI, Bst28I, BstNZ169I, Bsu15I, BsuTUI, ClaI, LcaI,
                     LplI, PgaI, Rme21I, SpmI, Ssp27144I, ZhoI"
                     /note="Geneious type: restriction site"
     misc_feature    4278..4292
                     /Recognition_pattern="GGANNNNNNNNATGC"
                     /annotation_group="GGANNNNNNNNATGC"
                     /label="Eco1323I, Eco1344II, Eco1386I, Eco1427I,
                     Eco1430I,Eco1456I, Eco1476I, Eco377I, Eco37I, EcoAO83I"
                     /note="Geneious type: restriction site"
     misc_feature    4677..4682
                     /Recognition_pattern="GGTAC^C"
                     /Methylation="Dcm:cut, CpG:cut"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    4677..4682
                     /Recognition_pattern="GGTACC"
                     /annotation_group="GGTACC"
                     /label="BspJ106I, Eco149I, Esp19I, KpnK14I, MvsAI, MvsBI,
                     MvsCI, MvsDI, MvsEI, MvsI, NmiI, Sau10I, SthAI, SthBI,
                     SthCI, SthDI, SthEI, SthFI, SthGI, SthHI, SthJI, SthKI,
                     SthLI, SthMI, SthNI, Uba1201I, Uba76I, Uba85I, Uba86I,
                     Uba87I"
                     /note="Geneious type: restriction site"
     misc_feature    4677..4682
                     /Recognition_pattern="G^GTACC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGTACC"
                     /label="Acc65I, AhaB8I, Asp718I, SthI"
                     /note="Geneious type: restriction site"
     misc_feature    4729..4735
                     /Recognition_pattern="GTTCNAG"
                     /annotation_group="GTTCNAG"
                     /label="Lra68I"
                     /note="Geneious type: restriction site"
     misc_feature    4836..4846
                     /Recognition_pattern="RTACNNNNGTG"
                     /annotation_group="RTACNNNNGTG"
                     /label="EcoMII"
                     /note="Geneious type: restriction site"
     misc_feature    4982..4996
                     /Recognition_pattern="CCANNNNN^NNNNTGG"
                     /Methylation="Dcm:cut"
                     /annotation_group="CCANNNNNNNNNTGG"
                     /label="XcmI"
                     /note="Geneious type: restriction site"
     misc_feature    5005..5011
                     /Recognition_pattern="GATCGAC"
                     /annotation_group="GATCGAC"
                     /label="NpeUS61II"
                     /note="Geneious type: restriction site"
     misc_feature    5005..5011
                     /Recognition_pattern="GATCNAC"
                     /annotation_group="GATCNAC"
                     /label="Aod1I"
                     /note="Geneious type: restriction site"
     misc_feature    5023..5033
                     /Recognition_pattern="CCTNNNNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="Bpu1268I, BsoEI, BstWI, Uba1289I, Uba1290I,
                     Uba1308I, Uba1309I, Uba1310I"
                     /note="Geneious type: restriction site"
     misc_feature    5023..5033
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCTNNNNNAGG"
                     /label="BstENI, EcoNI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5168..5174)
                     /Recognition_pattern="CGRGGAC(20/18)"
                     /annotation_group="CGRGGAC"
                     /label="RpaB5I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5213..5218)
                     /Recognition_pattern="CTGGAC"
                     /annotation_group="CTGGAC"
                     /label="BspGI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5246..5252)
                     /Recognition_pattern="CTTCGAG"
                     /annotation_group="CTTCGAG"
                     /label="RspPBTS2III"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5342..5353)
                     /Recognition_pattern="CACNNNNNTGGC"
                     /annotation_group="CACNNNNNTGGC"
                     /label="Eco912I"
                     /note="Geneious type: restriction site"
     misc_feature    5344..5350
                     /Recognition_pattern="CRAGCAC"
                     /annotation_group="CRAGCAC"
                     /label="Lsp6406VI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5351..5357)
                     /Recognition_pattern="RGCCCAC"
                     /annotation_group="RGCCCAC"
                     /label="PflPt14I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5359..5364)
                     /Recognition_pattern="TAGGAG"
                     /annotation_group="TAGGAG"
                     /label="Bce3081I"
                     /note="Geneious type: restriction site"
     misc_feature    5400..5405
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="CpG:some impaired"
                     /annotation_group="CCCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    5400..5405
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    5400..5405
                     /Recognition_pattern="CCCGGG"
                     /annotation_group="CCCGGG"
                     /label="CphBI, KteAI, Uba1220I, Uba1393I"
                     /note="Geneious type: restriction site"
     misc_feature    5400..5405
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="AhyI, Cfr9I, EaeAI, EclRI, Pac25I, PspAI, TspMI,
                     XcyI, XmaCI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    5400..5405
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="CfrJ4I, PaeBI, PspALI, SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5462..5467)
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACGTC"
                     /label="AjiI, BmgBI, BtrI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5657..5663)
                     /Recognition_pattern="GGACGAC"
                     /annotation_group="GGACGAC"
                     /label="Sth20745III"
                     /note="Geneious type: restriction site"
     misc_feature    5834..5839
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BbvAIII, BlfI, BseAI, BsiMI,
                     Bsp13I, BspEI, BspMII, Bsu23I, CauB3I, Kpn2I, MroI,
                     PinBII, PtaI"
                     /note="Geneious type: restriction site"
     misc_feature    5834..5839
                     /Recognition_pattern="TCCGGA"
                     /annotation_group="TCCGGA"
                     /label="Bbf7411I, Bla7920I, BsiGI, BsiOI, Bsp228I,
                     Bsp233I, Bsp508I, BspH226I, BstZ1I, BstZ3I, Bsu22I,
                     Cfr57I, EspHK26I, Tsp507I, Tsp514I, Uba1136I, Uba1279I,
                     Uba1375I, Uba1425I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5846..5852)
                     /Recognition_pattern="CGCCGAC"
                     /annotation_group="CGCCGAC"
                     /label="PliMI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5846..5851)
                     /Recognition_pattern="GCCGAC"
                     /annotation_group="GCCGAC"
                     /label="Exi27195I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5846..5851)
                     /Recognition_pattern="GCCGAC(21/19)"
                     /annotation_group="GCCGAC"
                     /label="NmeA6CIII"
                     /note="Geneious type: restriction site"
     misc_feature    6011..6021
                     /Recognition_pattern="(8/13)AAGNNNNNCTT(13/8)"
                     /annotation_group="AAGNNNNNCTT"
                     /label="FalI"
                     /note="Geneious type: restriction site"
     misc_feature    6347..6352
                     /Recognition_pattern="TACGAC(20/18)"
                     /annotation_group="TACGAC"
                     /label="DrdIV"
                     /note="Geneious type: restriction site"
     misc_feature    6728..6733
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="EcoRI"
     misc_feature    6748..7344
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="WPRE"
     misc_feature    7517..7548
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="polypurine tract (PPT)"
     misc_feature    7865..8045
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="3' LTR"
     misc_feature    8077..8301
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BGH polyadenylation sequence"
     misc_feature    8364..8670
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="f1_origin"
     misc_feature    8784..8804
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pBABE_3_primer"
     misc_feature    8790..9005
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SV40_enhancer"
     misc_feature    8802..9070
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SV40_promoter"
     misc_feature    8969..9046
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SV40_origin"
     misc_feature    9031..9050
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SV40pro_F_primer"
     misc_feature    9164..9231
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="EM7_promoter"
     misc_feature    9232..9606
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Sh ble (Zeocin Resistance)"
     misc_feature    9739..9858
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SV40_PA_terminator"
     misc_feature    9902..9920
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="M13_reverse_primer"
     misc_feature    10293..10912
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pBR322 origin"
     misc_feature    11067..11927
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Ampicllin Resistance (Rev-Com)"
     misc_feature    11969..11997
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Ampicillin Promoter"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacga caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccaccat gccgtacatc tttgcctttt tctgcaccgg
     3241 ttttctaggt gcggttgtgg gtgccaatgc gcgaggtgga ggcggtgggg gagggggtgg
     3301 aggaacgcgt agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     3361 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     3421 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     3481 aacacttgtc actactttaa cttatggtgt tcaatgcttt tcaagatacc cagatcatat
     3541 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     3601 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     3661 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     3721 acacaaattg gaatacaact ataacgatca ccaggtgtac atcatggcag acaaacaaaa
     3781 gaatggaatc aaagctaact tcaaaattag acacaacatt gaagatggag gcgttcaact
     3841 agcagaccat tatcaacaaa atactccaat tggcgatggg cccgtccttt taccagacaa
     3901 ccattacctg tttacaactt ctactctttc gaaagatccc aacgaaaaga gagaccacat
     3961 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     4021 aacgcgtgga ggtggagggg gcggaggtgg cgggggttcg cgcttcccca acaatatcca
     4081 gatagggggg ttatttccaa accaacaatc acaggaacat gcggctttta ggtttgcttt
     4141 gtcacaactc acggagcccc ccaagctgct tccccagatc gatattgtga acatcagcga
     4201 cagctttgag atgacttacc gtttctgttc ccagttctcc aaaggagtct atgccatctt
     4261 tggattttat gaacgaagga ctgtcaacat gctgacctcc ttctgtgggg ccctccatgt
     4321 gtgcttcatt actccaagtt ttcctgttga cacatccaat caatttgtcc ttcagctacg
     4381 cccggaacta caggaagctc tcattagcat tatcgaccat tacaagtggc aaacctttgt
     4441 ctacatttat gatgctgacc ggggcctgtc agtcctgcag agagtcttgg atacagccgc
     4501 agagaagaac tggcaggtaa cggctgtcaa cattctgaca accaccgagg aaggataccg
     4561 gatgctcttt caggacctgg agaagaaaaa ggagaggctg gtggtggttg actgtgaatc
     4621 agaacgcctc aacgccatcc tgggccagat cgtgaagcta gaaaagaatg gcatcgggta
     4681 ccactacatc ctcgccaatc tgggcttcat ggacattgac ttaaataagt tcaaggagag
     4741 cggagccaat gtgacaggtt tccagctggt gaactacaca gacacgatcc cagccagaat
     4801 catgcagcaa tggaggacaa gtgactcccg agaccatacc agggtggact ggaagaggcc
     4861 aaagtacact tctgctctca cctatgatgg tgtcaaggtg atggctgagg ccttccaaag
     4921 cctgcggagg cagaggattg acatatcccg ccgggggaat gctggggact gtctggctaa
     4981 cccagctgtg ccctggggtc aagggatcga catccagaga gccctgcagc aggtgcgctt
     5041 cgaaggtttg acaggaaatg tgcagttcaa cgagaaaggg cgccggacca attacaccct
     5101 ccacgtgatc gaaatgaaac atgatggaat ccgaaagatt ggttactgga atgaagacga
     5161 taaatttgtc cccgcagcca ccgacgctca ggctggaggg gacaactcaa gcgtccagaa
     5221 taggacctac atcgtcacta ctatcctcga agatccttac gtgatgctta aaaagaatgc
     5281 caaccagttt gagggcaatg accgctatga gggctactgt gtggagctgg ctgcagagat
     5341 cgccaagcac gtgggctact cctaccgact tgagattgtc agcgacggca aatatggagc
     5401 ccgggatccc gacacaaagg cttggaatgg catggtggga gaactggtct atggaagagc
     5461 agacgtggct gtggctccct tgaccataac cttggtccgg gaggaagtca tcgacttctc
     5521 caagccattc atgagtttgg gaatctccat tatgattaag aagccacaga agtccaagcc
     5581 aggtgtcttc tcctttcttg accctttggc ctatgagatc tggatgtgta tagtgtttgc
     5641 ctacattgga gtgagcgtcg tcctcttcct ggtcagccgt ttcagcccct acgaatggca
     5701 cagcgaagag tttgaagagg gacgagacca gacaaccagt gaccagtcaa atgagtttgg
     5761 catattcaac agcctgtggt tctccctggg ggccttcatg cagcaaggat gtgacatttc
     5821 ccccaggtcc ctgtccggac gcatcgtcgg cggcgtctgg tggttcttca ctttgatcat
     5881 catctcctcg tacacagcca acctggctgc cttcctgact gtggagagga tggtgtctcc
     5941 cattgagagt gcagaggacc tggcaaagca gacggaaatt gcttatggga cattggaagc
     6001 aggctccact aaggagttct tcaggagatc taaaatcgct gtgtttgaga agatgtggac
     6061 atacatgaag tctgcagaac catccgtgtt tgttcggacc acagaggaag gcatgatcag
     6121 agtgagaaaa tctaaaggca aatacgccta cctcctggag tccaccatga atgagtatat
     6181 tgagcaacga aagccctgtg acaccatgaa agtgggaggt aacttggatt ccaaaggcta
     6241 tggcattgcg acacccaagg ggtccgccct gagaaatcca gtaaacctgg cagtgttaaa
     6301 actgaacgag caggggcttt tggacaaatt gaaaaacaaa tggtggtacg acaagggcga
     6361 gtgcggcagc gggggaggtg actccaagga caagaccagc gctttgagcc tcagcaatgt
     6421 ggcaggcgtg ttctacatcc tgattggagg gctgggactg gccatgctgg ttgccttaat
     6481 cgagttctgc tacaaatccc gtagcgagtc gaagcggatg aagggtttct gtttgatccc
     6541 acagcaatcc atcaatgaag ccatacggac atcgaccctc ccccggaaca gtggggcagg
     6601 agccagcgga ggaggcggca gtggagagaa tggccgggtg gtcagccagg acttccccaa
     6661 gtccatgcaa tccattccct gcatgagtca cagttcaggg atgcccttgg gagccacagg
     6721 attgtaagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     6781 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6841 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6901 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6961 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7021 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7081 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7141 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7201 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7261 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7321 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     7381 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     7441 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     7501 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7561 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     7621 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     7681 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     7741 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     7801 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     7861 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8041 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     8101 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8161 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8221 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8281 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     8341 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8401 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8461 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8521 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8581 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8641 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8701 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8761 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8821 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8881 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8941 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9001 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9061 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9121 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     9181 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     9241 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     9301 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     9361 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     9421 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     9481 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     9541 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     9601 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     9661 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9721 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9841 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9961 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10021 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10081 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10141 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10201 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10981 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11041 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
