LOCUS       pMpGWB123_-_pPIP1;4-NLS-pENTRDTOPO_Expression_clone       14321 bp    DNA     circular UNA 18-NOV-2022
DEFINITION  an expression clone created from pMpGWB123 and C23p-NLS-pENTRDTOPO.
ACCESSION   urn.local...6p-fkbzu7y
VERSION     urn.local...6p-fkbzu7y
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    54..78
                     /note="<html><body>right border repeat from nopaline C58
                     T-DNA</body></html>"
                     /standard_name="RB T-DNA repeat"
     primer_bind     266..288
                     /standard_name="M13/pUC Forward"
     primer_bind     280..297
                     /standard_name="M13 Forward"
     primer_bind     281..297
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 fwd"
     protein_bind    323..>330
                     /bound_moiety="<html><body>LR Clonase™</body></html>"
                     /gene="<html><body>mutant version
                     of&nbsp;<i>att</i>R</body></html>"
                     /note="<html><body>recombination site for the Gateway® LR
                     reaction</body></html>"
                     /standard_name="attR1"
     misc_feature    324..344
                     /note="Geneious type: attB1"
                     /standard_name="attB1"
     misc_feature    357..>367
                     /standard_name="F"
     misc_feature    364..367
                     /standard_name="CACC"
     misc_feature    368..2867
                     /standard_name="c23upstream2500"
     misc_feature    complement(<2868..2884)
                     /standard_name="R"
     misc_feature    2868..2870
                     /standard_name="ATG"
     CDS             <2871..2891
                     /codon_start=1
                     /standard_name="SV40 NLS"
     misc_feature    complement(2911..2931)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     protein_bind    complement(<2918..2932)
                     /bound_moiety="<html><body>LR Clonase™</body></html>"
                     /gene="<html><body>mutant version of
                     <i>att</i>R</body></html>"
                     /note="<html><body>recombination site for the Gateway® LR
                     reaction</body></html>"
                     /standard_name="attR2"
     CDS             join(2964..2966,2967..2969,2970..3680)
                     /codon_start=1
                     /note="<html><body>mammalian
                     codon-optimized</body></html>"
                     /product="<html><body>enhanced variant of YFP (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/11050231""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/11050231"">Heik
                     al et al., 2001</a>)</body></html>"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK"
                     /standard_name="Citrine"
     primer_bind     complement(3270..3289)
                     /standard_name="EXFP-R"
     primer_bind     3617..3638
                     /standard_name="EGFP-C"
     CDS             join(3702..3704,3705..3707,3708..4418)
                     /codon_start=1
                     /note="<html><body>mammalian
                     codon-optimized</body></html>"
                     /product="<html><body>enhanced variant of YFP (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/11050231""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/11050231"">Heik
                     al et al., 2001</a>)</body></html>"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK"
                     /standard_name="Citrine"
     CDS             join(4440..4442,4443..4445,4446..5156)
                     /codon_start=1
                     /note="<html><body>mammalian
                     codon-optimized</body></html>"
                     /product="<html><body>enhanced variant of YFP (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/11050231""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/11050231"">Heik
                     al et al., 2001</a>)</body></html>"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK"
                     /standard_name="Citrine"
     regulatory      5181..5433
                     /note="Geneious type: terminator"
                     /note="<html><body>nopaline synthase terminator and
                     poly(A) signal</body></html>"
                     /standard_name="NOS terminator"
     primer_bind     complement(5467..5483)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 rev"
     primer_bind     complement(5467..5483)
                     /standard_name="M13 Reverse"
     primer_bind     complement(5480..5502)
                     /standard_name="M13/pUC Reverse"
     protein_bind    5491..5507
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /standard_name="lac operator"
     regulatory      join(complement(5540..5545),complement(5522..5539),
                     complement(5515..5521))
                     /note="Geneious type: promoter"
                     /note="<html><body>promoter for the <i>E. coli lac</i>
                     operon</body></html>"
                     /standard_name="lac promoter"
     protein_bind    5560..5581
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /standard_name="CAP binding site"
     misc_feature    5666..5840
                     /note="Geneious type: polyA_signal"
                     /note="<html><body>cauliflower mosaic virus
                     polyadenylation signal</body></html>"
                     /standard_name="CaMV poly(A) signal"
     CDS             complement(5881..6906)
                     /codon_start=1
                     /gene="<html><body><i>aph(4)-Ia</i></body></html>"
                     /note="<html><body>confers resistance to
                     hygromycin</body></html>"
                     /product="<html><body>aminoglycoside phosphotransferase
                     from <i>E. coli</i></body></html>"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKK*"
                     /standard_name="HygR"
     regulatory      complement(6973..7648)
                     /note="Geneious type: promoter"
                     /note="<html><body>cauliflower mosaic virus 35S promoter
                     with a duplicated enhancer region</body></html>"
                     /standard_name="CaMV 35S promoter (enhanced)"
     misc_feature    7843..7867
                     /note="<html><body>left border repeat from nopaline C58
                     T-DNA</body></html>"
                     /standard_name="LB T-DNA repeat"
     CDS             8388..9179
                     /codon_start=1
                     /gene="<html><body><i>aadA</i></body></html>"
                     /note="<html><body>confers resistance to spectinomycin and
                     streptomycin</body></html>"
                     /product="<html><body>aminoglycoside adenylyltransferase
                     (<a href=""http://www.ncbi.nlm.nih.gov/pubmed/2993813""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/2993813"">Murph
                     y, 1985</a>)</body></html>"
                     /transl_table=1
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
                     /standard_name="SmR"
     rep_origin      9425..10013
     primer_bind     9914..9933
                     /standard_name="pBR322ori-F"
     primer_bind     10167..10184
                     /standard_name="L4440"
     misc_feature    10199..10339
                     /note="<html><body>basis of mobility region from
                     pBR322</body></html>"
                     /standard_name="bom"
     primer_bind     complement(10265..10284)
                     /standard_name="pRS-marker"
     primer_bind     10425..10447
                     /standard_name="pGEX 3'"
     rep_origin      10683..10877
     CDS             complement(10943..12016)
                     /codon_start=1
                     /note="<html><body><br></body></html>"
                     /product="<html><body>replication protein from
                     the&nbsp;<i>Pseudomonas</i> plasmid pVS1 (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10659714""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10659714"">Heeb
                     et al., 2000</a>)</body></html>"
                     /protein_id="<html><body><br></body></html>"
                     /transl_table=1
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS*"
                     /standard_name="pVS1 RepA"
     primer_bind     12281..12291
                     /standard_name="mCherry-R"
     CDS             complement(12445..13074)
                     /codon_start=1
                     /product="<html><body>stability protein from
                     the&nbsp;<i>Pseudomonas</i> plasmid pVS1 (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10659714""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10659714"">Heeb
                     et al., 2000</a>)</body></html>"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /standard_name="pVS1 StaA"
     primer_bind     12711..12723
                     /standard_name="EGFP-N"
ORIGIN      
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
      361 cttcacccga actttactag ttactatcta ccaatcaggt tatgaaagta ggcaaaaggg
      421 gaagggtcaa ggcccatgag acatattttc caaatagaca ctaaaaatga gccagatttt
      481 tcaacaacac atttggttta agggctcaaa aaatatttgt caacacccag ttttaaaaca
      541 aaagaaaata gtaaattaca ggcccaaatg tttttgttgc cctatagata ttagtatttc
      601 ttaaccagtc gctgataatt attgatgaat agttgagaga cgataaagat tgagctgtca
      661 ggaatacaaa tcaaacatga tattgttcgt ttacattatt actcaacgaa cattcacgtt
      721 tccaaattct gatatctttt ctaatatttc tccacatgta gcatatcatt agttactgtt
      781 taaaaatgag taccaaaaac gtgaaattat tacaataacg tataaatgtt aaatatttcg
      841 agcaatcaat acttgtcaag actcattgtt agtaatgcat cactataatt cattcttttt
      901 ttgtcttggt caaacatgta tgaagcgagc agagtctatg aatgtcatat tatagacata
      961 cgtatgcggc tttggtcggg accggcccaa taaaacaata cacatgttca acgggttctt
     1021 ccacgtgtca aacttatgtt ggctggacgg tatgtgactg tgcattgtgg ttacgtttcc
     1081 aaaatgaata cgtaagaact tttcctaaat tatgtctttt gcacaatact gtaagtctgt
     1141 aactatggta ctaaacatta taatatcgaa ttggaaacat ttacatttga gaattttagt
     1201 gtttgttggc aacgaagcat gtgagatcac agagaagcaa tactcatctc tgagctgttc
     1261 aaacagtttt acatgtcagc acccaaatat agtcactata ttcaaaaaca aaattattta
     1321 cttgcattca atgctagcct tttttattcg gtccacaaca aattataaat atttgtatat
     1381 aagaaaaatc agagacctga ttttgtaatg cgtggcttgt caatttcaag caacacagaa
     1441 aaaagctaac taagcaaaaa acaaaaaaaa ataacaagtg gctggttgat agaatgtatc
     1501 tttcatcagt acataacact gtttttttgt caacgtaaaa cagattttta taacttttgt
     1561 ttgccctcaa cttttttcat tacattttct ggaatatcgc atggttgaat aattaaaagt
     1621 taaaaatgat ttttatgaag cacggcatta tactatatag tcagtcccaa cgaaaatgac
     1681 tttacataaa gctgtcaatt ttttctccaa cggtcagata attgtttcgt tgaatagtca
     1741 gttttttttt tactatttta ctattgggct ttattgggct cttacaattt tatcgatttt
     1801 tgtttaccgt ccagaaagtg gaccctgtgg gaagaggaaa agaaaaaaaa agggtcaggt
     1861 tttttgtctt tatacgagag cacatcggta cgttccagaa caaccctctc tagtctcctc
     1921 catcattatc tttatttaat agtaacccta gtacaaatta ccaaactatt caacattaac
     1981 ataattattc aacattcacc atcatgaata ataactagtt gagaaaaacg taactagact
     2041 agtttgatct gtcaacataa aaagagaaaa aagaatttcg tgactagtaa agtttaagac
     2101 cattcacaaa tgatcattga aattattagt tctaaactct tagtttggtt atctatttgg
     2161 ttttgattcg tatctatttg gttattcggt tttttggttt taaagtttag tattcattcg
     2221 actattttga aattttggtt cgtttttttt tttttttttg aaatttaata tccattcgat
     2281 tattttgctt ttaatatttt taaatatttc aattattttt tgatattttt tgaatattgt
     2341 tactatttaa tatatatttt taaatatttt taattactaa gtatagaaat aactaatatt
     2401 tttgaaaata aaattatatt ttggatattt tagatcatct aaaatatttt agttaggtac
     2461 taattcattt ttggttattt tgattataaa atttaatatc tattcaacaa tttttaaatt
     2521 gttgattcaa ttcggttctg tttttttttt tcatttaggt ttagtacatt tggttcagtt
     2581 tatttgactc tcagtttatt tgcctaagcc ttgaaattag gtgttaacta ttggttattc
     2641 tagttgaaat ttgaaataaa gaaaaaacta tacgttatac tattttattt tcttagcaat
     2701 aactaaaaat aaatgtttgg aaacgttagg caattttatt atattctatc gttgttctct
     2761 ataaatgaag tctcgaccac acacttggta gctacacacg agaaagaaaa agctcacaac
     2821 tttaaccttt ctggagagag aagaacaaaa gagggagagg gagagaaatg ccaaagaaga
     2881 agagaaaggt caagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgataaca
     2941 gcgctggagg ctccggtggg agcatggtga gcaagggcga ggagctgttc accggggtgg
     3001 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3061 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3121 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
     3181 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3241 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3301 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3361 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3421 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3481 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3541 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     3601 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3661 gcatggacga gctgtacaag gctggaggct ccggtgggag catggtgagc aagggcgagg
     3721 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3781 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3841 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     3901 acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3961 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4021 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4081 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4141 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4201 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4261 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     4321 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4381 ccgccgggat cactctcggc atggacgagc tgtacaaggc tggaggctcc ggtgggagca
     4441 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4501 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4561 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4621 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
     4681 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4741 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4801 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4861 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4921 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4981 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5041 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5101 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     5161 cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     5221 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     5281 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     5341 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     5401 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     5461 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5521 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5581 aggctgaatt aggcgcgcct atttctgaat tcaacacatt gcggacgttt ttaatgtact
     5641 gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga
     5701 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
     5761 tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact
     5821 cacacattat tatggagaaa ctcgagcttg tcgatcgaca gatcccggtc ggcatctact
     5881 ctatttcttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     5941 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     6001 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     6061 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     6121 gagtcgtggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     6181 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     6241 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     6301 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     6361 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     6421 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     6481 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     6541 gatcgcatcc atagcctccg cgaccggttg tagaacagcg ggcagttcgg tttcaggcag
     6601 gtcttgcaac gtgacaccct gtgaacggcg ggagatgcaa taggtcaggc tctcgctaaa
     6661 ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     6721 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     6781 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     6841 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     6901 tttcatatct cattgccccc cggatctgcg aaagctcgag agagatagat ttgtagagag
     6961 agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     7021 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa
     7081 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     7141 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     7201 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     7261 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     7321 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     7381 tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     7441 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     7501 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     7561 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     7621 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     7681 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca
     7741 atacgcaaac cgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa
     7801 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     7861 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     7921 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     7981 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     8041 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     8101 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     8161 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     8221 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     8281 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     8341 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
     8401 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     8461 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     8521 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     8581 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     8641 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     8701 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     8761 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     8821 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     8881 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     8941 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     9001 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     9061 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     9121 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     9181 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     9241 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     9301 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     9361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9541 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     9601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10201 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
    10261 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
    10321 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    10381 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    10441 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
    10501 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
    10561 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
    10621 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
    10681 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
    10741 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
    10801 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
    10861 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
    10921 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
    10981 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
    11041 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
    11101 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
    11161 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
    11221 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
    11281 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
    11341 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
    11401 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
    11461 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
    11521 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
    11581 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
    11641 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
    11701 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    11761 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    11821 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    11881 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    11941 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    12001 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    12061 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    12121 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    12181 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    12241 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    12301 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    12361 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    12421 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    12481 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    12541 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    12601 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    12661 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    12721 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    12781 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    12841 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    12901 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    12961 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    13021 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    13081 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    13141 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    13201 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    13261 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    13321 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    13381 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    13441 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    13501 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    13561 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    13621 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    13681 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    13741 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    13801 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    13861 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    13921 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    13981 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    14041 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    14101 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    14161 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    14221 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    14281 gtggttggca tgcacataca aatggacgaa cggataaacc t
//
