LOCUS       Exported                6397 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5459 bp ds-DNA     circular SYN 
            16-JUN-2017

            DEFINITION  GFP expression in forebrain GABA-ergic interneurons 
            under the 

                        control of the mDlx enhancer element.

            ACCESSION   .

            VERSION     .

            KEYWORDS    pAAV-mDlx-GFP-Fishell-1

            SOURCE      synthetic DNA construct

              ORGANISM  synthetic DNA construct

            REFERENCE   1  (bases 1 to 5459)

              AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi 
            GA, Guo L, Xu 

                        Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, 
            Jacob AL, Smith 

                        GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, 
            Kotak VC, 

                        Mowery TM, Anderson SA, Callaway EM, Dasen JS, 
            Fitzpatrick D, 

                        Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, 
            Rudy B, Feng G,

                        Fishell G

              TITLE     A viral strategy for targeting and manipulating 
            interneurons across 

                        vertebrate species.

              JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.

              PUBMED    27798629

            REFERENCE   2  (bases 1 to 5459)

              AUTHORS   .

              TITLE     Direct Submission

              JOURNAL   Exported Friday, Jun 16, 2017 from SnapGene Console 
            4.0.0a8

                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="synthetic DNA construct"
                     /label=synthetic DNA construct
     repeat_region   1..141
                     /label=inverted terminal repeat of
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(23..34)
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     primer_bind     84..95
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     enhancer        170..699
                     /label=mDlx
                     /note="mDlx"
     primer_bind     170..191
                     /label=TATACACTCACAGTGGTTTGGC
     misc_feature    631..652
                     /label=mDlx-1c
     primer_bind     complement(678..699)
                     /label=CTTCCTACTGTGAAACTTTGGG
     misc_feature    847..868
                     /label=mDlx-2c
                     /note="mDlx-2c"
     intron          860..992
                     /label=chimera between introns from human
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     975..994
                     /label=Synthetic intron, forward primer
                     /note="Synthetic intron, forward primer"
     misc_feature    1040..1966
                     /label=Aion
     misc_feature    complement(1190..1211)
                     /label=Phobos-2nc
     misc_feature    1421..1423
                     /label=C128A
     misc_feature    1505..1507
                     /label=D156C
                     /note="D156C"
     misc_feature    1514..1516
                     /label=T177G
     misc_feature    1526..1528
                     /label=G181A
     misc_feature    1979..2698
                     /label=Citrine
     misc_feature    complement(2042..2063)
                     /label=Citrine-1nc
     misc_feature    2562..2582
                     /label=Citrine-2c
     misc_feature    2743..3331
                     /label=woodchuck hepatitis virus
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2796..2816)
                     /label=WPRE, reverse primer
                     /note="WPRE, reverse primer"
     CDS             complement(3214..3225)
                     /codon_start=1
                     /label=Factor Xa recognition and cleavage site
                     /translation="IEGR"
     polyA_signal    3500..3581
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3537..3556)
                     /label=SV40 polyA, reverse primer
                     /note="SV40 polyA, reverse primer"
     primer_bind     3591..3610
                     /label=SV40 polyA terminator, reverse primer
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3660..3800
                     /label=inverted terminal repeat of
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(3706..3717)
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     primer_bind     3767..3778
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     rep_origin      3875..4330
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of replication
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3962..3981)
                     /label=F1 origin, reverse primer
                     /note="F1 origin, reverse primer"
     primer_bind     4172..4193
                     /label=F1 origin, forward primer
                     /note="F1 origin, forward primer"
     primer_bind     complement(4347..4366)
                     /label=pRS vectors
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4466..4488
                     /label=pGEX vectors, reverse primer
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4526..4544)
                     /label=pBR322 vectors
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4612..4716
                     /label=bla
     CDS             4717..5577
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4935..4954)
                     /label=Ampicillin resistance gene
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5748..6336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6237..6256
                     /label=pBR322 origin, forward primer
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taagaattct atacactcac
      181 agtggtttgg catatatttg gtgaaatttt ttaaggaaaa attagtgttg gtttcgatat
      241 atggtagctt tttctctaac ataatttgaa taattcagca aagccctact accagctgta
      301 cttctgcagc ctcttccatt ctttccagca ttataatttt ggttaatttt caattttagg
      361 tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt tgtttcgcct
      421 ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga agaaaattac
      481 aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa aaattactct
      541 aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa accttaaatt
      601 gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt tcagaattga
      661 tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcga attcctgcag
      721 cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctagcct
      781 gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct gcagaagttg
      841 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      901 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      961 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1021 ttaagaaact agcgccacca tggactatgg cggcgctttg tctgccgtcg gactcttcca
     1081 aacctcatac actcttgaga acaatggttc tgttatttgc atcccgaaca acggccagtg
     1141 cttctgcttg gcttggctta aatccaacgg aacaaatgcc gagaagttgg ctgccaacat
     1201 tctgcagtgg atttcttttg cgctttcagc gctctgcctg atgttctacg gctaccagac
     1261 ctggaagtct acttgcggct gggagaacat ttacgtggcc acgatccaga tgatcaagtt
     1321 catcatcgag tatttccata gctttgacga acctgcggtg atctactcat ccaacggcaa
     1381 caagacccgg tggcttcgtt acgcgagctg gctgctgacc gcccctgtca ttcttatcca
     1441 cctgagcaac cttacgggtc tggcgaacga ctataacaag cgtaccatgg gtctgctggt
     1501 gtcatgcatc ggcggcatcg tgtgggccac cacggccgcg ctgtccaagg gatacgtccg
     1561 tgtcattttc ttcctgatgg gcctgtgcta cggcatctac acattcttca acgcagccaa
     1621 ggtctacatt gaggcgtacc acaccgtgcc gaagggccgg tgtcgccagg tggtgactgg
     1681 catggcttgg ctcttcttcg tatcatgggg tatgttcccc atcctgttca tcctcggccc
     1741 cgagggcttc ggcgtcctga gccggtacgg ctccaacgtc ggccacacca tcattgacct
     1801 gatgtcgaag cagtgctggg gtctgctcgg ccactacctg cgcgtgctga tccacagcca
     1861 tatcctcatc cacggcgaca ttcgcaagac caccaaattg aacattggtg gcactgagat
     1921 tgaggtcgag acgctggtgg aggacgaggc cgaggctggc gcggtcgcgg cactcgagat
     1981 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2041 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2101 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2161 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     2221 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2281 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2341 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2401 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2461 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2521 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2581 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2641 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     2701 gcgcgccacc cctgcaggga attcgatatc aagcttatcg ataatcaacc tctggattac
     2761 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2821 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2881 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2941 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3001 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3061 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3121 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg
     3181 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3241 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3301 agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct
     3361 gtgatagcgg ccatcaagct ggccgcgact ctagatcata atcagccata ccacatttgt
     3421 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3481 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3601 caaactcatc aatgtatcag cttatcgata ccgcatgcac gtgcggaccg agcggccgca
     3661 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     3721 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     3781 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3841 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     3901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4081 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4201 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4321 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4381 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4441 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4501 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4561 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4921 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5881 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6361 cggttcctgg ccttttgctg gccttttgct cacatgt
//
