LOCUS       Exported                7470 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       #097\pAAV-Ef-1a-DIO\iChloC\2A\RFP        7293 bp    DNA 
               circular     11-JAN-2016

            SOURCE      

              ORGANISM  

            COMMENT     This file is created by Vector NTI

                        http://www.invitrogen.com/

            COMMENT     ORIGDB|GenBank

            COMMENT     VNTDATE|-11058445|

            COMMENT     VNTDBDATE|-11058445|

            COMMENT     LSOWNER|

            COMMENT     VNTNAME|#097 pAAV-Ef-1a-DIO iChloC 2A RFP|

            COMMENT     VNTAUTHORNAME|Iris Ohmert|

            COMMENT     VNTAUTHORTEL|004940741055085|

            COMMENT     VNTAUTHORFAX|004940741058364|

            COMMENT     VNTAUTHOREML|iohmert@zmnh.uni-hamburg.de|

            COMMENT     VNTAUTHORAD1|Zentrum fuer Molekulare Neurobiologie 
            Hamburg|

            COMMENT     VNTAUTHORAD2|Institut fuer Synaptische Physiologie|

            COMMENT     VNTAUTHORAD3|Falkenried 94/Martinistr.85|

            COMMENT     VNTAUTHORAD4|D-20251 Hamburg|

            COMMENT     VNTOAUTHORNAME|ISP|

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            COMMENT                          ""Created: 01/11/16 09:40"" 1) 

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            COMMENT                            1 5 ""TCTAGA"" ""AGATCT"") 

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FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        249..1419
                     /label=EF1a Promoter
     misc_feature    1288..1308
                     /label=EF1a-1c
                     /note="EF1a-1c"
     misc_feature    1450..1483
                     /label=LoxP 2722
     misc_feature    1534..1567
                     /label=loxP
     misc_feature    complement(1588..1782)
                     /label=stKv2.1 GCopt
                     /note="stKv2.1 GCopt"
     misc_feature    complement(1783..2499)
                     /label=Citrine-GCopt
                     /note="Citrine-GCopt"
     misc_feature    complement(1896..1916)
                     /label=Citrine-2c
                     /note="Citrine-2c"
     misc_feature    1974..1994
                     /label=Citrine-GCopt-1nc
     misc_feature    complement(2512..3438)
                     /label=phobos
     misc_feature    complement(2600..2622)
                     /label=Phobos-1c
                     /note="Phobos-1c"
     misc_feature    complement(2950..2952)
                     /label=G181A
     misc_feature    complement(2962..2964)
                     /label=T177G
     misc_feature    complement(2971..2973)
                     /label=D156C
                     /note="D156C"
     misc_feature    complement(3055..3057)
                     /label=C128A
     misc_feature    3455..3488
                     /label=loxP 2722
     misc_feature    complement(3539..3572)
                     /label=loxP
     misc_feature    3593..4188
                     /label=WRE
     misc_feature    complement(3690..3711)
                     /label=WPRE-2nc
                     /note="WPRE-2nc"
     misc_feature    4215..4692
                     /label=hGH PolyA
     misc_feature    4733..4872
                     /label=right ITR
     rep_origin      4965..5270
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5790..6643
                     /label=AmpR
     rep_origin      6798..7464
                     /direction=RIGHT
                     /label=pUC Origin
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg
     1561 aagttatggc gcgagctttc tagatcagaa gcgggtggcc tcggggaagt cggtggcgca
     1621 gctgatgaag ctgtcgatgc tgctcatgct gcgcatgtcg atcacgccct cggtgcgggc
     1681 gggcaggggg gccacggggc tgggcatgct gctcatctcc agctcctcgg ggggcttgct
     1741 ctggggggcc atctccttgg tgttcaggat gggctggctc tgcttgtaca gctcgtccat
     1801 gcccagggtg atgccggcgg cggtcacgaa ctccagcagc accatgtggt cgcgcttctc
     1861 gttggggtcc ttgctcaggg cgctctggta gctcaggtag tggttgtcgg gcagcagcac
     1921 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gccagctgca cgctgccgtc
     1981 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     2041 gatgtacacg ttgtggctgt tgtagttgta ctccagcttg tggcccagga tgttgccgtc
     2101 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     2161 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctgcac
     2221 gtagccctcg ggcatggcgc tcttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     2281 ggcgaagcac atcaggccgt agccgaaggt ggtcaccagg gtgggccagg gcacgggcag
     2341 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcgt cgccctcgcc
     2401 ctcgccgctc acgctgaact tgtggccgtt cacgtcgccg tccagctcca ccaggatggg
     2461 caccacgccg gtgaacagct cctcgccctt gctcaccacc tcgagtgccg cgaccgcgcc
     2521 agcctcggcc tcgtcctcca ccagcgtctc gacctcaatc tcagtgccac caatgttcaa
     2581 tttggtggtc ttgcgaatgt cgccgtggat gaggatatgg ctgtggatca gcacgcgcag
     2641 gtagtggccg agcagacccc agcactgctt cgacatcagg tcaatgatgg tgtggccgac
     2701 gttggagccg taccggctca ggacgccgaa gccctcgggg ccgaggatga acaggatggg
     2761 gaacataccc catgatacga agaagagcca agccatgcca gtcaccacct ggcgacaccg
     2821 gcccttcggc acggtgtggt acgcctcaat gtagaccttg gctgcgttga agaatgtgta
     2881 gatgccgtag cacaggccca tcaggaagaa aatgacacgg acgtatccct tggacagcgc
     2941 ggccgtggtg gcccacacga tgccgccgat gcatgacacc agcagaccca tggtacgctt
     3001 gttatagtcg ttcgccagac ccgtaaggtt gctcaggtgg ataagaatga caggggcggt
     3061 cagcagccag ctcgcgtaac gaagccaccg ggtcttgttg ccgttggatg agtagatcac
     3121 cgcaggttcg tcaaagctat ggaaatactc gatgatgaac ttgatcatct ggatcgtggc
     3181 cacgtaaatg ttctcccagc cgcaagtaga cttccaggtc tggtagccgt agaacatcag
     3241 gcagagcgct gaaagcgcaa aagaaatcca ctgcagaatg ttggcagcca acttctcggc
     3301 atttgttccg ttggatttaa gccaagccaa gcagaagcac tggccgttgt tcgggatgca
     3361 aataacagaa ccattgttct caagagtgta tgaggtttgg aagagtccga cggcagacaa
     3421 agcgccgcca tagtccatgg tggcgctagc tagcataact tcgtataaag tatcctatac
     3481 gaagttattt gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagaat
     3541 aacttcgtat aatgtatgct atacgaagtt atgaattcga tatcaagctt atcgataatc
     3601 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3661 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3721 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3781 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3841 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3901 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3961 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4021 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4081 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4141 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     4201 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct
     4261 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt
     4321 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg
     4381 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc
     4441 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc
     4501 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt
     4561 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca
     4621 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
     4681 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     4741 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4801 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4861 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4921 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5161 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5281 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5401 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5581 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5641 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6961 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7441 tggccttttg ctggcctttt gctcacatgt
//
