LOCUS       Exported                5505 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       D602_peAAV-CKIIa(0.4)-M 5609 bp ds-DNA     circular     
            17-DEC-2018

            DEFINITION  .

            KEYWORDS    ""creator:Tivan27"" ""marker:ER, AAV2 ITR, AAV2 ITR, 
            AmpR""
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="synthetic DNA construct"
                     /label=peAAV-CKIIa(0.4)-MosOPN3-ts-mScarlet-ER
     repeat_region   1..141
                     /label=AAV2 ITR
     promoter        156..518
                     /label=a-CaMKII(0.4)
     misc_feature    274..292
                     /label=IO-035_S_CamK-Cterm.F
     gene            531..1457
                     /label=phobos
     misc_feature    complement(681..702)
                     /label=Phobos-2nc
                     /note="Phobos-2nc"
     misc_feature    912..914
                     /label=C128A
     misc_feature    996..998
                     /label=D156C
                     /note="D156C"
     misc_feature    1005..1007
                     /label=T177G
     misc_feature    1017..1019
                     /label=G181A
     misc_feature    1347..1369
                     /label=Phobos-1c
     CDS             1470..1529
                     /label=ts
     misc_feature    1533..2249
                     /label=Citrine
     misc_feature    complement(1596..1617)
                     /label=Citrine-1nc
                     /note="Citrine-1nc"
     misc_feature    2116..2136
                     /label=Citrine-2c
     CDS             2250..2270
                     /label=ER
     misc_feature    2280..2683
                     /label=WPRE
     misc_feature    complement(2361..2381)
                     /label=WPRE-4nc
                     /note="WPRE-4nc"
     polyA_signal    2690..2738
     repeat_region   2768..2908
                     /label=AAV2 ITR
     rep_origin      2983..3438
                     /label=f1 ori
     promoter        3720..3824
                     /label=AmpR promoter
     CDS             3825..3893
                     /label=AmpR
     CDS             3894..4685
                     /label=AmpR
     rep_origin      4856..5444
                     /label=ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcttgt ggactaagtt tgttcgcatc
      181 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      241 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      301 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      361 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      421 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
      481 gcacgggcag gcgagtggcc cctagttctg ggggcagctc tagcgccacc atggactatg
      541 gcggcgcttt gtctgccgtc ggactcttcc aaacctcata cactcttgag aacaatggtt
      601 ctgttatttg catcccgaac aacggccagt gcttctgctt ggcttggctt aaatccaacg
      661 gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg gatttctttt gcgctttcag
      721 cgctctgcct gatgttctac ggctaccaga cctggaagtc tacttgcggc tgggagaaca
      781 tttacgtggc cacgatccag atgatcaagt tcatcatcga gtatttccat agctttgacg
      841 aacctgcggt gatctactca tccaacggca acaagacccg gtggcttcgt tacgcgagct
      901 ggctgctgac cgcccctgtc attcttatcc acctgagcaa ccttacgggt ctggcgaacg
      961 actataacaa gcgtaccatg ggtctgctgg tgtcatgcat cggcggcatc gtgtgggcca
     1021 ccacggccgc gctgtccaag ggatacgtcc gtgtcatttt cttcctgatg ggcctgtgct
     1081 acggcatcta cacattcttc aacgcagcca aggtctacat tgaggcgtac cacaccgtgc
     1141 cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg
     1201 gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg agccggtacg
     1261 gctccaacgt cggccacacc atcattgacc tgatgtcgaa gcagtgctgg ggtctgctcg
     1321 gccactacct gcgcgtgctg atccacagcc atatcctcat ccacggcgac attcgcaaga
     1381 ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg
     1441 ccgaggctgg cgcggtcgcg gcactcgaga agagcaggat caccagcgag ggcgagtaca
     1501 tccccctgga ccagatcgac atcaacgtgg tgatggtgag caagggcgag gagctgttca
     1561 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1621 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1681 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga
     1741 tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1801 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1861 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1921 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1981 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2041 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2101 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     2161 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2221 tcactctcgg catggacgag ctgtacaagt tctgctacga gaacgaggtg taaaagctta
     2281 atcaacctct ggattacaaa atttgtgaaa gattgactga tattcttaac tatgttgctc
     2341 cttttacgct gtgtggatat gctgctttaa tgcctctgta tcatgctatt gcttcccgta
     2401 tggctttcgt tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2461 ggcccgttgt ccgtcaacgt ggcgtggtgt gctctgtgtt tgctgacgca acccccactg
     2521 gctggggcat tgccaccacc tgtcaactcc tttctgggac tttcgctttc cccctcccga
     2581 tcgccacggc agaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctaggttgc
     2641 tgggcactga taattccgtg gtgttgtcgg ggaagctgac gtcagcgcta ataaaatcgc
     2701 tatccatcga agatggatgt gtgttggttt tttgtgtggg taacccacgt gcggaccgag
     2761 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     2821 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     2881 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     2941 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3001 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3061 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3181 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3301 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     3361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3421 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3481 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3601 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     3661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
