LOCUS       Exported                6980 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       D602_peAAV-CKIIa(0.4)-M 5609 bp ds-DNA     circular     
            17-DEC-2018

            DEFINITION  .

            KEYWORDS    ""creator:Tivan27"" ""marker:ER, AAV2 ITR, AAV2 ITR, 
            AmpR""
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="synthetic DNA construct"
                     /label=D602_peAAV-CKIIa(0.4)-M
     repeat_region   1..141
                     /label=AAV2 ITR
     promoter        156..518
                     /label=a-CaMKII(0.4)
     misc_feature    274..292
                     /label=IO-035
                     /note="IO-035"
     gene            539..1411
                     /label=GtACR2
                     /note="GtACR2"
     gene            1427..1486
                     /label=TS
                     /note="TS"
     gene            1496..2206
                     /label=mCerulean3
                     /note="mCerulean3"
     misc_feature    2076..2096
                     /label=Citrine-2c
     gene            2213..2524
                     /label=beta subunit HK-ATPase
                     /note="beta subunit HK-ATPase"
     misc_feature    2519..2539
                     /label=beta-1c
                     /note="beta-1c"
     CDS             2567..3538
                     /codon_start=1
                     /label=ChroME2f
                     /translation="ETAATMTHAFISAVPSAEATIRGLLSAAAVVTPAADAHGETSNAT
                     TAGADHGCFPHINHGTELQHKIAVGLQWFTVIVAIVQLIFYGWHSFKATTGWEEVYVCV
                     IELVKCFIELFHDVDSPATVYQTNGGAVAWLRYSEWLLTCPVILIHLSNLTGLHEEYSK
                     RTMTILVTDIGNIVWGITAAFTKGPLKILFFMIGLFYGVTCFFQIAKVYIESYHTLPKG
                     VCRKICKIMAYVFFCSWLMFPVMFIAGHEGLGLITPYTSGIGHLILDLIAKNTWGFLGH
                     HLRVKIHEHILIHGDIRKTTTINVAGENMEIETFVDEEEEGGV"
     misc_feature    2915..2917
                     /label=Untitled
     misc_feature    2963..2965
                     /label=Untitled
     misc_feature    complement(3032..3053)
                     /label=ChroME2-1nc
                     /note="ChroME2-1nc"
     misc_feature    3545..3739
                     /label=stKv2.1 GCopt
                     /note="stKv2.1 GCopt"
     misc_feature    3755..4158
                     /label=WPRE
     misc_feature    complement(3836..3856)
                     /label=WPRE-4nc
                     /note="WPRE-4nc"
     polyA_signal    4165..4213
     repeat_region   4243..4383
                     /label=AAV2 ITR
     rep_origin      4458..4913
                     /direction=RIGHT
                     /label=f1 ori
     misc_feature    complement(4517..4537)
                     /label=f1-ori-3nc
     promoter        5195..5299
                     /label=AmpR promoter
     CDS             5300..5368
                     /label=AmpR
     CDS             5369..6160
                     /label=AmpR
     rep_origin      6331..6919
                     /direction=RIGHT
                     /label=ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcttgt ggactaagtt tgttcgcatc
      181 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      241 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      301 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      361 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      421 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
      481 gcacgggcag gcgagtggcc cctagttctg ggggcagctc tagagcggta ccggatccat
      541 ggcatcacag gtcgtctatg gggaatgggc atcaactcac accgagtgct acaatatgtc
      601 cagaatcgac tccaccttcg tctcactgct gcagctggtg tgggccgtgg tctcaggctg
      661 ccagacaatc ttcatgatta gcagggctcc caaggtccct tgggagtccg tgtacctgcc
      721 ctttgtcgaa tctatcactt atgcactggc cagtactggc aacggaaccc tgcagatgag
      781 ggacggcaga ttctttcctt ggagccgaat ggcctcctgg ctgtgcacct gtccaatcat
      841 gctgggacag atttctaaca tggctctggt gaagtacaaa agtatcccac tgaatcccat
      901 tgcacaggcc gcttccatca ttagagtggt catgggaatc accgctacaa tttctcccgc
      961 agagtatatg aagtggctgt tctttttctt tggggccacc tgcctggtgt tcgagtacag
     1021 tgtggtcttc acaatctttc aagtggggct gtatggcttt gaatctgtcg ggacacctct
     1081 ggcacagaag gtggtggtga ggatcaagat gctgcgcctg atcttcttta ttgcttggac
     1141 tatgttccca atcgtgtggc tgatttcacc caccggagtc tgcgtgatcc acgagaacgt
     1201 gagcgccatt ctgtacctgc tggctgacgg cctgtgcaaa aatacatatg gagtgatcct
     1261 gtggagcact gcctggggag tgctggaagg gaagtgggac cccgcatgtc tgccagggca
     1321 ggagaaaccc gaagccgacg atccttttgg cctgaatcac gagaagaatg cccctcctaa
     1381 cgacgaagtc aatatcagaa tgtttggaag gggacgtacg gcggccaaga gcaggatcac
     1441 cagcgagggc gagtacatcc ccctggacca gatcgacatc aacgtggcgg tacccgtgag
     1501 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1561 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1621 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1681 caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1741 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1801 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1861 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1921 gtacaacgcc atccacggca acgtctatat caccgccgac aagcagaaga acggcatcaa
     1981 ggccaacttc ggcctcaact gcaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2041 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2101 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2161 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtataccg gtgcagccct
     2221 gcaggagaag aagtcatgca gccagcgcat ggccgaattc cggcaatact gttggaaccc
     2281 ggacactggg cagatgctgg gccgcacccc agcccggtgg gtgtggatca gcctgtacta
     2341 tgcagctttc tacgtggtca tgactgggct ctttgccttg tgcatctatg tgctgatgca
     2401 gaccattgat ccctacaccc ccgactacca ggaccagtta aagtcaccgg gggtaacctt
     2461 gagaccggat gtgtatgggg aaagagggct gcagatttcc tacaacatct ctgaaaacag
     2521 ctctagacag gcccagatca ccggacgtcc ggagggagct gtacaagaaa cagccgccac
     2581 aatgacccac gcctttatct cagccgtgcc tagcgccgaa gccacaatta gaggcctgct
     2641 gagcgccgca gcagtggtga caccagcagc agacgctcac ggagaaacct ctaacgccac
     2701 aacagccgga gccgatcacg gttgcttccc ccacatcaac cacggaaccg agctgcagca
     2761 caagatcgca gtgggactcc agtggttcac cgtgatcgtg gctatcgtgc agctcatctt
     2821 ctacggttgg cacagcttca aggccacaac cggctgggag gaggtctacg tctgcgtgat
     2881 cgagctcgtc aagtgcttca tcgagctgtt ccacgatgtc gacagcccag ccacagtgta
     2941 ccagaccaac ggaggagccg tggcttggct gcggtacagc gagtggctcc tgacttgccc
     3001 cgtgatcctg atccacctga gcaacctgac cggactgcac gaagagtaca gcaagcggac
     3061 catgaccatc ctggtgaccg acatcggcaa catcgtgtgg gggatcacag ccgcctttac
     3121 aaagggcccc ctgaagatcc tgttcttcat gatcggcctg ttctacggcg tgacttgctt
     3181 cttccagatc gccaaggtgt atatcgagag ctaccacacc ctgcccaaag gcgtctgccg
     3241 gaagatttgc aagatcatgg cctacgtctt cttctgctct tggctgatgt tccccgtgat
     3301 gttcatcgcc ggacacgagg gactgggcct gatcacacct tacaccagcg gaatcggcca
     3361 cctgatcctg gatctgatcg ccaagaacac ttggggcttc ctgggccacc acctgagagt
     3421 gaagatccac gagcacatcc tgatccacgg cgacatccgg aagacaacca ccatcaacgt
     3481 ggccggcgag aacatggaga tcgagacctt cgtcgacgag gaggaggagg gaggagtgct
     3541 cgagcagagc cagcccatcc tgaacaccaa ggagatggcc ccccagagca agccccccga
     3601 ggagctggag atgagcagca tgcccagccc cgtggccccc ctgcccgccc gcaccgaggg
     3661 cgtgatcgac atgcgcagca tgagcagcat cgacagcttc atcagctgcg ccaccgactt
     3721 ccccgaggcc acccgcttct gatctagaaa gcttaatcaa cctctggatt acaaaatttg
     3781 tgaaagattg actgatattc ttaactatgt tgctcctttt acgctgtgtg gatatgctgc
     3841 tttaatgcct ctgtatcatg ctattgcttc ccgtatggct ttcgttttct cctccttgta
     3901 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtccgtc aacgtggcgt
     3961 ggtgtgctct gtgtttgctg acgcaacccc cactggctgg ggcattgcca ccacctgtca
     4021 actcctttct gggactttcg ctttccccct cccgatcgcc acggcagaac tcatcgccgc
     4081 ctgccttgcc cgctgctgga caggggctag gttgctgggc actgataatt ccgtggtgtt
     4141 gtcggggaag ctgacgtcag cgctaataaa atcgctatcc atcgaagatg gatgtgtgtt
     4201 ggttttttgt gtgggtaacc cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     4261 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4321 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4381 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4441 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4741 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     4801 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4861 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4921 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4981 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5041 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5101 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5161 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5221 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5281 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5341 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5401 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5461 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5521 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5581 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5641 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5701 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5761 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5821 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5881 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5941 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6001 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6061 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6121 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6181 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6241 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6301 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6361 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6421 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6481 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6541 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6601 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6661 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6721 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6781 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6841 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6901 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6961 ctggcctttt gctcacatgt
//
