LOCUS       Exported                4848 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5459 bp ds-DNA     circular SYN 
            16-JUN-2017

            DEFINITION  GFP expression in forebrain GABA-ergic interneurons 
            under the 

                        control of the mDlx enhancer element.

            ACCESSION   .

            VERSION     .

            KEYWORDS    pAAV-mDlx-GFP-Fishell-1

            SOURCE      synthetic DNA construct

              ORGANISM  synthetic DNA construct

            REFERENCE   1  (bases 1 to 5459)

              AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi 
            GA, Guo L, Xu 

                        Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, 
            Jacob AL, Smith 

                        GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, 
            Kotak VC, 

                        Mowery TM, Anderson SA, Callaway EM, Dasen JS, 
            Fitzpatrick D, 

                        Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, 
            Rudy B, Feng G,

                        Fishell G

              TITLE     A viral strategy for targeting and manipulating 
            interneurons across 

                        vertebrate species.

              JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.

              PUBMED    27798629

            REFERENCE   2  (bases 1 to 5459)

              AUTHORS   .

              TITLE     Direct Submission

              JOURNAL   Exported Friday, Jun 16, 2017 from SnapGene Console 
            4.0.0a8

                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="synthetic DNA construct"
                     /label=pAAV-PRSX8-GFP
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    165..186
                     /label=PRSX8-1c
     promoter        166..396
                     /label=PRSX8-Promoter
     misc_feature    180..202
                     /label=1st
     misc_feature    203..225
                     /label=2nd
     misc_feature    227..248
                     /label=3rd
     misc_feature    249..270
                     /label=4th
     misc_feature    295..317
                     /label=6th
     misc_feature    318..340
                     /label=7th
     misc_feature    341..362
                     /label=8th
     CDS             430..1149
                     /codon_start=1
                     /label=enhanced GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(475..496)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(736..755)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1083..1104
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1194..1782
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1247..1267)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     misc_feature    complement(1287..1308)
                     /label=WPRE-2nc
     CDS             complement(1665..1676)
                     /codon_start=1
                     /label=Factor Xa recognition and cleavage site
                     /translation="IEGR"
     polyA_signal    1951..2032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1988..2007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2042..2061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2111..2251
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2326..2781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2413..2432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2623..2644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2798..2817)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2917..2939
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2977..2995)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3063..3167
                     /label=bla
     CDS             3168..4028
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3386..3405)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4199..4787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4688..4707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taaaaacgcg tattagcttc
      181 cgctagacaa atgtgattac ccccgctaga caaatgtgat tacccgcgct agacaaatgt
      241 gattaccccg ctagacaaat gtgattaccc cccgctagac aaatgtgatt acccccgcta
      301 gacaaatgtg attacccgcg ctagacaaat gtgattaccc cgctagacaa atgtgattac
      361 ccccgaccag ggcataaatg gccaggtggg accagagata tcgctagcaa ggatccacta
      421 gtagccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      481 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      541 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      601 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      661 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      721 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      781 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      841 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      901 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      961 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1021 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1081 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1141 tacaagtaaa ggcgcgccac ccctgcaggg aattcgatat caagcttatc gataatcaac
     1201 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     1261 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     1321 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     1381 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     1441 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     1501 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     1561 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg
     1621 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     1681 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     1741 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc
     1801 tcgagagatc tgtgatagcg gccatcaagc tggccgcgac tctagatcat aatcagccat
     1861 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     1921 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     1981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2041 tgtggtttgt ccaaactcat caatgtatca gcttatcgat accgcatgca cgtgcggacc
     2101 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2161 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2221 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     2281 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     2341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2521 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2641 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     2701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2761 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     2821 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2941 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3001 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3061 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4321 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
