LOCUS       Exported                5964 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5459 bp ds-DNA     circular SYN 
            16-JUN-2017

            DEFINITION  GFP expression in forebrain GABA-ergic interneurons 
            under the 

                        control of the mDlx enhancer element.

            ACCESSION   .

            VERSION     .

            KEYWORDS    pAAV-mDlx-GFP-Fishell-1

            SOURCE      synthetic DNA construct

              ORGANISM  synthetic DNA construct

            REFERENCE   1  (bases 1 to 5459)

              AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi 
            GA, Guo L, Xu 

                        Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, 
            Jacob AL, Smith 

                        GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, 
            Kotak VC, 

                        Mowery TM, Anderson SA, Callaway EM, Dasen JS, 
            Fitzpatrick D, 

                        Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, 
            Rudy B, Feng G,

                        Fishell G

              TITLE     A viral strategy for targeting and manipulating 
            interneurons across 

                        vertebrate species.

              JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.

              PUBMED    27798629

            REFERENCE   2  (bases 1 to 5459)

              AUTHORS   .

              TITLE     Direct Submission

              JOURNAL   Exported Friday, Jun 16, 2017 from SnapGene Console 
            4.0.0a8

                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="synthetic DNA construct"
                     /label=Untitled
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    165..186
                     /label=PRSX8-1c
     promoter        166..396
                     /label=PRSX8-Promoter
     misc_feature    180..202
                     /label=1st
     misc_feature    203..225
                     /label=2nd
     misc_feature    227..248
                     /label=3rd
     misc_feature    249..270
                     /label=4th
     misc_feature    295..317
                     /label=6th
     misc_feature    318..340
                     /label=7th
     misc_feature    341..362
                     /label=8th
     gene            417..1289
                     /label=GtACR2
                     /note="GtACR2"
     misc_feature    1256..1276
                     /label=GtACR2-2c
                     /note="GtACR2-2c"
     gene            1305..1364
                     /label=TS
                     /note="TS"
     CDS             1374..2087
                     /codon_start=1
                     /label=enhanced GFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    complement(1416..1437)
                     /label=EGFP-nc
                     /note=" EGFP-nc"
     misc_feature    2024..2045
                     /label=EGFP-c
                     /note="EGFP-c"
     misc_feature    2094..2288
                     /label=stKv2.1 GCopt
                     /note="stKv2.1 GCopt"
     misc_feature    2310..2898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    complement(2403..2424)
                     /label=WPRE-2nc
     CDS             complement(2781..2792)
                     /codon_start=1
                     /label=Factor Xa recognition and cleavage site
                     /translation="IEGR"
     polyA_signal    3067..3148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3104..3123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3158..3177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3227..3367
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3442..3897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3529..3548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3739..3760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3914..3933)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4033..4055
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4093..4111)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4179..4283
                     /label=bla
     CDS             4284..5144
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4502..4521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5315..5903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5804..5823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taaaaacgcg tattagcttc
      181 cgctagacaa atgtgattac ccccgctaga caaatgtgat tacccgcgct agacaaatgt
      241 gattaccccg ctagacaaat gtgattaccc cccgctagac aaatgtgatt acccccgcta
      301 gacaaatgtg attacccgcg ctagacaaat gtgattaccc cgctagacaa atgtgattac
      361 ccccgaccag ggcataaatg gccaggtggg accagagata tcgctagcaa ggatccatgg
      421 catcacaggt cgtctatggg gaatgggcat caactcacac cgagtgctac aatatgtcca
      481 gaatcgactc caccttcgtc tcactgctgc agctggtgtg ggccgtggtc tcaggctgcc
      541 agacaatctt catgattagc agggctccca aggtcccttg ggagtccgtg tacctgccct
      601 ttgtcgaatc tatcacttat gcactggcca gtactggcaa cggaaccctg cagatgaggg
      661 acggcagatt ctttccttgg agccgaatgg cctcctggct gtgcacctgt ccaatcatgc
      721 tgggacagat ttctaacatg gctctggtga agtacaaaag tatcccactg aatcccattg
      781 cacaggccgc ttccatcatt agagtggtca tgggaatcac cgctacaatt tctcccgcag
      841 agtatatgaa gtggctgttc tttttctttg gggccacctg cctggtgttc gagtacagtg
      901 tggtcttcac aatctttcaa gtggggctgt atggctttga atctgtcggg acacctctgg
      961 cacagaaggt ggtggtgagg atcaagatgc tgcgcctgat cttctttatt gcttggacta
     1021 tgttcccaat cgtgtggctg atttcaccca ccggagtctg cgtgatccac gagaacgtga
     1081 gcgccattct gtacctgctg gctgacggcc tgtgcaaaaa tacatatgga gtgatcctgt
     1141 ggagcactgc ctggggagtg ctggaaggga agtgggaccc cgcatgtctg ccagggcagg
     1201 agaaacccga agccgacgat ccttttggcc tgaatcacga gaagaatgcc cctcctaacg
     1261 acgaagtcaa tatcagaatg tttggaaggg gacgtacggc ggccaagagc aggatcacca
     1321 gcgagggcga gtacatcccc ctggaccaga tcgacatcaa cgtggcggta cccgtgagca
     1381 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1441 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1501 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1561 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1621 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1681 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1741 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1801 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1861 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1921 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1981 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2041 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagacc ggtcagagcc
     2101 agcccatcct gaacaccaag gagatggccc cccagagcaa gccccccgag gagctggaga
     2161 tgagcagcat gcccagcccc gtggcccccc tgcccgcccg caccgagggc gtgatcgaca
     2221 tgcgcagcat gagcagcatc gacagcttca tcagctgcgc caccgacttc cccgaggcca
     2281 cccgcttctg atctagaaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     2341 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2401 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2461 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2521 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2581 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2641 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2701 ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga
     2761 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2821 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2881 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctgtg atagcggcca
     2941 tcaagctggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     3001 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3061 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3181 gtatcagctt atcgataccg catgcacgtg cggaccgagc ggccgcagga acccctagtg
     3241 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3301 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     3361 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3421 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     3661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3781 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3841 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     3901 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3961 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4021 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4081 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4141 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4201 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4261 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4321 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4381 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4441 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4501 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4561 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4621 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4681 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4741 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4801 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4861 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4921 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4981 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5041 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5101 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5461 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5701 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5941 tttgctggcc ttttgctcac atgt
//
