LOCUS       Exported                6212 bp ds-DNA     circular SYN 26-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5459 bp ds-DNA     circular SYN 
            16-JUN-2017

            DEFINITION  GFP expression in forebrain GABA-ergic interneurons 
            under the 

                        control of the mDlx enhancer element.

            ACCESSION   .

            VERSION     .

            KEYWORDS    pAAV-mDlx-GFP-Fishell-1

            SOURCE      synthetic DNA construct

              ORGANISM  synthetic DNA construct

            REFERENCE   1  (bases 1 to 5459)

              AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi 
            GA, Guo L, Xu 

                        Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, 
            Jacob AL, Smith 

                        GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, 
            Kotak VC, 

                        Mowery TM, Anderson SA, Callaway EM, Dasen JS, 
            Fitzpatrick D, 

                        Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, 
            Rudy B, Feng G,

                        Fishell G

              TITLE     A viral strategy for targeting and manipulating 
            interneurons across 

                        vertebrate species.

              JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.

              PUBMED    27798629

            REFERENCE   2  (bases 1 to 5459)

              AUTHORS   .

              TITLE     Direct Submission

              JOURNAL   Exported Friday, Jun 16, 2017 from SnapGene Console 
            4.0.0a8

                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="synthetic DNA construct"
                     /label=synthetic DNA construct
     repeat_region   1..141
                     /label=inverted terminal repeat of
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(23..34)
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     primer_bind     84..95
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     misc_feature    165..186
                     /label=PRSX8-1c
                     /note="PRSX8-1c"
     promoter        166..396
                     /label=PRSX8-Promoter
     misc_feature    180..202
                     /label=1st
     misc_feature    203..225
                     /label=2nd
     misc_feature    227..248
                     /label=3rd
     misc_feature    249..270
                     /label=4th
     misc_feature    295..317
                     /label=6th
     misc_feature    318..340
                     /label=7th
     misc_feature    341..362
                     /label=8th
     regulatory      403..412
                     /label=other
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    409..2538
                     /label=Untitled
     CDS             409..1758
                     /codon_start=1
                     /label=GCaMP7b
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNPPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMYARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      505..514
                     /label=other
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     795..816
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(922..943)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    1759..1767
                     /label=GSG Linker
     misc_feature    1768..1824
                     /label=P2A
     misc_feature    1825..2538
                     /label=mRuby3
     misc_feature    2558..3146
                     /label=woodchuck hepatitis virus
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2611..2631)
                     /label=WPRE, reverse primer
                     /note="WPRE, reverse primer"
     misc_feature    complement(2651..2672)
                     /label=WPRE-2nc
                     /note="WPRE-2nc"
     CDS             complement(3029..3040)
                     /codon_start=1
                     /label=Factor Xa recognition and cleavage site
                     /translation="IEGR"
     polyA_signal    3315..3396
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3352..3371)
                     /label=SV40 polyA, reverse primer
                     /note="SV40 polyA, reverse primer"
     primer_bind     3406..3425
                     /label=SV40 polyA terminator, reverse primer
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3475..3615
                     /label=inverted terminal repeat of
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(3521..3532)
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     primer_bind     3582..3593
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     rep_origin      3690..4145
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of replication
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3777..3796)
                     /label=F1 origin, reverse primer
                     /note="F1 origin, reverse primer"
     primer_bind     3987..4008
                     /label=F1 origin, forward primer
                     /note="F1 origin, forward primer"
     primer_bind     complement(4162..4181)
                     /label=pRS vectors
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4281..4303
                     /label=pGEX vectors, reverse primer
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4341..4359)
                     /label=pBR322 vectors
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4427..4531
                     /label=bla
     CDS             4532..5392
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4750..4769)
                     /label=Ampicillin resistance gene
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5563..6151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6052..6071
                     /label=pBR322 origin, forward primer
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taaaaacgcg tattagcttc
      181 cgctagacaa atgtgattac ccccgctaga caaatgtgat tacccgcgct agacaaatgt
      241 gattaccccg ctagacaaat gtgattaccc cccgctagac aaatgtgatt acccccgcta
      301 gacaaatgtg attacccgcg ctagacaaat gtgattaccc cgctagacaa atgtgattac
      361 ccccgaccag ggcataaatg gccaggtggg accagagatc tggccaccat gggttctcat
      421 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      481 tacgacgatg acgataagga tctcgccacc atggtcgact catcacgtcg taagtggaat
      541 aagacaggtc acgcagtcag agctataggt cggctgagct cactcgagaa cgtctatatc
      601 aaggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
      661 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
      721 gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac
      781 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      841 atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg
      901 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      961 agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1021 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1081 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1141 atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1201 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gcttaagggc
     1261 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cccacctgac
     1321 caactgactg aagagcagat cgcagaattt aaagagcttt tctccctatt tgacaaggac
     1381 ggggatggga caataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac
     1441 cccacagaag cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca
     1501 atcgacttcc ctgagttcct gacaatgtac gcaagaaaaa tgaaatacag ggacacggaa
     1561 gaagaaatta gagaagcgtt cggtgtgttt gataaggatg gcaatggcta catcagtgca
     1621 gcagagcttc gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat
     1681 gaaatgatca gggaagcaga catcgatggg gatggtcagg taaactacga agagtttgta
     1741 caaatgatga cagcgaaggg atccggagcc acgaacttct ctctgttaaa gcaagcagga
     1801 gacgtggaag aaaaccccgg tcctatggtg tctaagggcg aagagctgat caaggaaaat
     1861 atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt
     1921 gaaggagaag gcagaccgta cgagggaact caaaccatga ggatcaaagt catcgaggga
     1981 ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtact
     2041 tttatcaagt acccggccga catccctgat ttctttaaac agtcctttcc tgagggtttt
     2101 acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcac gcaggacacc
     2161 agccttgagg atggcgagct cgtctacaac gtcaaggtca gaggggtaaa ctttccctcc
     2221 aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat
     2281 ccagcagatg gtggtctgag aggatacact gacatcgcac tgaaagttga tggtggtggc
     2341 catctgcact gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg gaacatcaag
     2401 atgcccggtg tccatgccgt tgatcaccgc ctggaaagga tcgaggagag tgacaatgaa
     2461 acctacgtag tgcaacgcga agtggcagtt gccaaataca gcaaccttgg tggtggcatg
     2521 gacgagctgt acaagtgagt ttatcaagct tatcgataat caacctctgg attacaaaat
     2581 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2641 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2701 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2761 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     2821 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     2881 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     2941 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     3001 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3061 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3121 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctgtgat
     3181 agcggccatc aagctggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     3241 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3301 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3361 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3421 tcatcaatgt atcagcttat cgataccgca tgcacgtgcg gaccgagcgg ccgcaggaac
     3481 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3541 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3601 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3661 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3841 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3901 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4021 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4081 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4141 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4201 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4261 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4321 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4381 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4501 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4561 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4621 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4681 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4741 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4801 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4861 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4921 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4981 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5041 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5101 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5161 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5221 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5281 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5341 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5701 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6181 cctggccttt tgctggcctt ttgctcacat gt
//
