LOCUS       Exported                6147 bp ds-DNA     circular SYN 08-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pOTTC1473 was constructed by Chris Richie at GE
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=ITR (AAV)
     promoter        156..1456
                     /label=a-CaMKII(1.3) promoter
     CDS             1500..2231
                     /label=KDELR2-Myc-DDK
     CDS             1500..2135
                     /label=hKDELR2
     protein_bind    2154..2183
                     /label=Myc epitope [EQKLISEEDL]
     protein_bind    2202..2225
                     /label=FLAG epitope / EK cleavage site [DYKDDDDK]
     misc_RNA        2264..2872
                     /label=WPRE
     polyA_signal    2893..3373
                     /label=hGH polyA
     LTR             complement(3410..3550)
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     rep_origin      3625..4080
                     /label=M13 origin
     rep_origin      3642..3948
                     /label=f1 origin
     promoter        4368..4466
                     /label=Bla promoter (prokaryotic)
     CDS             4467..5324
                     /label=AmpR
     rep_origin      5422..6104
                     /label=ColE1 origin
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccggatccga agttatcgct agctgccgcc gcgatcgcca
     1501 tgaacatttt ccggctgact ggggacctgt cccacctggc ggccatcgtc atcctgctgc
     1561 tgaagatctg gaagacgcgc tcctgcgccg gtatttctgg gaaaagccag cttctgtttg
     1621 cactggtctt cacaactcgt tacctggatc tttttacttc atttatttca ttgtataaca
     1681 catctatgaa ggttatctac cttgcctgct cctatgccac agtgtacctg atctacctga
     1741 aatttaaggc aacctacgat ggaaatcatg ataccttccg agtggagttt ctggtggtcc
     1801 ctgtgggagg cctctcattt ttagttaatc acgatttctc tcctcttgag atcctctgga
     1861 ccttctccat ctacctggag tccgtggcta tccttccgca gctgtttatg atcagcaaga
     1921 ctggggaggc cgagaccatc accacccact acctgttctt cctgggcctc tatcgtgctt
     1981 tgtatcttgt caactggatc tggcgcttct actttgaggg cttctttgac ctcattgctg
     2041 tggtggccgg cgtagtccag accatcctat actgtgactt cttctacttg tacattacaa
     2101 aagtactcaa gggaaagaag ctcagtttgc cagcaacgcg tacgcggccg ctcgagcaga
     2161 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     2221 ataaggttta aacggcgcgc cataacttcg aattcgatat caagcttatc gataatcaac
     2281 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2341 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2401 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2461 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2521 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2581 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2641 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2701 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2761 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2821 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc
     2881 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
     2941 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
     3001 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
     3061 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
     3121 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
     3181 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
     3241 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
     3301 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
     3361 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta
     3421 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3481 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     3541 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3601 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3841 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3961 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4021 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4081 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4261 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4321 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5641 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6121 ccttttgctg gccttttgct cacatgt
//
