LOCUS       Exported                9948 bp DNA     circular SYN 27-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1415..1648
                     /label=RRE
     misc_feature    2164..2281
                     /label=cPPT
     promoter        2409..3587
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_feature    3604..3609
                     /label=SpeI
     CDS             3613..4548
                     /codon_start=1
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     misc_feature    4552..4557
                     /label=SalI
     CDS             4558..4728
                     /codon_start=1
                     /label=CreN59 (3-59)
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLN"
     misc_feature    4729..4734
                     /label=KpnI
     CDS             4735..5184
                     /codon_start=1
                     /label=nMag
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     misc_feature    4779..5646
                     /label=seq conf MEA342
                     /note="4-28-18"
     misc_feature    4779..5623
                     /label=seq conf MEA342 col3
                     /note="4-17-18"
     misc_feature    5200..5205
                     /label=BamHI
     CDS             5209..5265
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5226..6007
                     /label=seq conf MEA103 col3
                     /note="4-17-18"
     misc_feature    5262..6026
                     /label=seq conf MEA103 maxiprep
                     /note="04-28-18"
     misc_feature    5266..5271
                     /label=MluI
     CDS             5272..5979
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    5983..5988
                     /label=EcoRI
     misc_feature    5999..6004
                     /label=XhoI
     misc_feature    6032..6037
                     /label=BamHI
     misc_feature    6064..6637
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6670..7269
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     misc_feature    7283..7288
                     /label=XhoI
     misc_feature    7401..7406
                     /label=KpnI
     LTR             7472..7705
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7837..7842
                     /label=XbaI
     polyA_signal    7862..8086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8123..8128
                     /label=XhoI
     promoter        8182..8286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8287..9147
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9318..9906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggtcg ataagctttg caaagatgga
     2341 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     2401 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     2761 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     2821 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     2881 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     2941 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     3001 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     3061 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     3121 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     3181 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3241 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3301 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3361 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3421 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3481 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     3541 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attgatcctt
     3601 cgaactagta tggctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc
     3661 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc
     3721 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt
     3781 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc
     3841 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt
     3901 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac
     3961 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta
     4021 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg
     4081 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg
     4141 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc
     4201 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag
     4261 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt
     4321 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac
     4381 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca
     4441 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc
     4501 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc tgtcgacaat
     4561 ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt
     4621 cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa
     4681 atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaatgg tacccatact
     4741 ctttatgccc ccggtggata tgacattatg ggatatctgg accagatcgg caaccggcca
     4801 aacccgcagg tggaactggg ccccgtggat acatcctgcg ccttgattct ttgtgacctg
     4861 aaacagaaag acaccccgat agtttacgcg agtgaagcct tcctctacat gacaggttac
     4921 agcaacgcag aggtgctggg ccggaattgc cggtttctgc aaagccctga cggcatggtg
     4981 aagcccaaga gcacccggaa gtacgtggat agtaacacaa tcaatactat gcgcaaggca
     5041 atcgacagga atgccgaggt gcaggttgaa gtagtcaatt ttaaaaagaa tggacagcga
     5101 tttgttaatt tcctgactat gatacctgtt agggacgaaa caggcgagta tcgatactct
     5161 atgggattcc agtgcgaaac agaaggaggg tctggagggg gatccggcgc caccaacttc
     5221 agcctgctga agcaggccgg cgacgtggag gagaaccccg gccccacgcg tgtgagcaag
     5281 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     5341 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     5401 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5461 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     5521 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     5581 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     5641 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     5701 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     5761 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     5821 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     5881 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     5941 cgccactcca ccggcggcat ggacgagctg tacaagtaat aggaattcga agcttgagct
     6001 cgagatctga gtccggtagc gctagttcga aggatccgca tgcatctagg gcggccaatt
     6061 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     6121 gtgtgcgttt gtctatatgt gattttccac catattgccg tcttttggca atgtgagggc
     6181 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     6241 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     6301 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     6361 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     6421 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     6481 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     6541 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     6601 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga
     6661 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6721 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6781 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6841 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6901 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6961 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7021 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7081 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7141 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7201 gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc
     7261 ggtgcctgat aataggcggc cgctcgagac ctagaaaaac atggagcaat cacaagtagc
     7321 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7381 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7441 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7501 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7561 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7621 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7681 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7741 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattataa tagatttagc
     7801 aggaattgaa ctaggagtgg agcacacagg caaagttcta gagctcgctg atcagcctcg
     7861 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7921 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7981 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     8041 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8101 agaaccagct gggggcgcgc ccctcgaggc cgccatggtc atagctgttt gacgtcaggt
     8161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     9721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgc
//
