LOCUS       pCK341_dSpRY            7368 bp ds-DNA     circular     29-SEP-2022
DEFINITION  .
KEYWORDS    "Parental Vector" "Origin" "Resistance" "Construction Method"
            "Promoter" "Terminator" "gRNA" "E.coli plasmids" "pCD"
COMMENT     Imported using the Genbank importer. File name: pCD442.ape New
            DNA(1) from 1 to 2574 New DNA from 1 to 2574
            /Users/Stanley/Desktop/pVKM112_PCR_SLQM20_SLQM21_BglII_XhoI.ape
            from 1 to 2574 ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature     6..111
                      /label="Phage lambda T0 terminator"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     misc_feature     complement(130..786)
                      /label="CmR (EcoRI-KO)"
                      /ApEinfo_revcolor="#000bff"
                      /ApEinfo_fwdcolor="#000bff"
     promoter         complement(853..884)
                      /label="pCat promoter (weak)"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer           complement(892..913)
                      /label="oCK249"
                      /note="sequence: GGGtgcgcccaatacgcacgatatctggcgaaaatgagac"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer           908..945
                      /label="oCD058"
                      /note="sequence: atatcgacgtcttaagTTACGAAATCATCCTGTGGAGC"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     promoter         924..1466
                      /label="endogenous promoter"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              1467..5573
                      /label="dSpRY-Cas9"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     CDS              1467..5573
                      /label="Translation 1467-5573"
     misc_feature     1494..1496
                      /label="D10A"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     primer           complement(1639..1682)
                      /label="oCK521"
                      /note="sequence: ATACCTTCTACGAGCTGTCCGTTTGAGACGAGTACGTTCCGCTG"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     misc_difference  1647..1649
                      /label="A61R"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     primer           complement(1659..1682)
                      /label="oCK492"
                      /note="sequence: ATACCTTCTACGAGCTGTCCGTTT"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     misc_difference  3984..3986
                      /label="H840A"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_difference  4797..4799
                      /label="L1111R"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     restriction site complement(4800..4813)
                      /label="AjuI"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     misc_difference  4869..4874
                      /label="D1135L/S1136W"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     misc_difference  5118..5123
                      /label="G1218K/E1219Q"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer           5387..5410
                      /label="oCK493"
                      /note="sequence: AAATATTATTCATTTATTTACGTT"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     misc_difference  5415..5417
                      /label="N1317R"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     misc_difference  5430..5432
                      /label="A1322R"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     misc_difference  5463..5465
                      /label="R1333P"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     misc_difference  5469..5471
                      /label="R1335Q"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  5475..5477
                      /label="T1337R"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer           complement(5551..5570)
                      /label="oCD228"
                      /note="sequence: GTCACCTCCTAGCTGACTCA"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     primer           complement(5575..5594)
                      /label="oCK219"
                      /note="sequence: GCCTGGagatccttactcga"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     terminator       5589..5656
                      /label="sglTerm"
                      /ApEinfo_revcolor="#800040"
                      /ApEinfo_fwdcolor="#800040"
     terminator       5589..5717
                      /label="dblTerm"
                      /ApEinfo_revcolor="#800080"
                      /ApEinfo_fwdcolor="#800080"
     promoter         5764..5798
                      /label="J23107 promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     misc_feature     5799..5825
                      /label="Bujard RBS (strong)"
                      /ApEinfo_revcolor="#db7aff"
                      /ApEinfo_fwdcolor="#db7aff"
     RBS              5808..5819
                      /label="RBS (strong)"
                      /ApEinfo_revcolor="#996633"
                      /ApEinfo_fwdcolor="#996633"
     primer           5819..5859
                      /label="oCD070"
                      /note="sequence: AGGTACCatggggcccgcttctaactttactcagttcgttc"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     misc_feature     5835..6182
                      /label="MCPdelFG"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer           6183..6197
                      /label="oCK088"
                      /note="sequence: GGTGGCGGAGGTAGC"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           complement(6183..6197)
                      /label="oCD444"
                      /note="sequence: GCTACCTCCGCCACC"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     primer           6197..6225
                      /label="oCK386"
                      /note="sequence: TTAAAGAGGAGAAAGGTACCATGTCCCATCAGAAAATTATTCAGGATC"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_feature     6198..6518
                      /label="SoxS_dmut"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              6198..6518
                      /label="Translation 6198-6518"
     misc_feature     6474..6476
                      /label="R93A"
                      /ApEinfo_revcolor="#ff0080"
                      /ApEinfo_fwdcolor="#ff0080"
     misc_feature     6498..6500
                      /label="S101A"
                      /ApEinfo_revcolor="#ff0080"
                      /ApEinfo_fwdcolor="#ff0080"
     primer           complement(6501..6518)
                      /label="oCD429"
                      /note="sequence: GCTACCTCCGCCACCCAGGCGGTGGCGATAATC"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer           complement(6501..6522)
                      /label="oCK385"
                      /note="sequence: gggttttttgcgtgGGCGCGCCTTACAGGCGGTGGCGATAATC"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     primer           complement(6532..6551)
                      /label="oCD276"
                      /note="sequence: ccgaagcggggttttttgcg"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     terminator       6532..6565
                      /label="BBa_B1002 terminator"
                      /ApEinfo_revcolor="#af14ff"
                      /ApEinfo_fwdcolor="#af14ff"
     primer           complement(6549..6565)
                      /label="oCK078"
                      /note="sequence: attacaacagtttttagcgaaaaaaccccgccg"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     primer           complement(6549..6566)
                      /label="oCDP058"
                      /note="sequence: CAAGGCCTTCGCGAGGCGAAAAAACCCCGCCG"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     6566..6571
                      /label="AvrII"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer           6567..6590
                      /label="oCK250"
                      /note="sequence: taaaaactgttgtaatctagggatatattccgcttcctcg"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     misc_feature     6719..7281
                      /label="p15A origin"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
       61 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      121 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      181 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      241 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      301 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
      361 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      421 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      481 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      541 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
      601 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      661 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      721 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      781 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      841 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
      901 tcgccagata tcgacgtctt aagTTACGAA ATCATCCTGT GGAGCTTAGT AGGTTTAGCA
      961 AGATGGCAGC GCCTAAATGT AGAATGATAA AAGGATTAAG AGATTAATTT CCCTAAAAAT
     1021 GATAAAACAA GCGTTTTGAA AGCGCTTGTT TTTTTGGTTT GCAGTCAGAG TAGAATAGAA
     1081 GTATCAAAAA AAGCACCGAC TCGGTGCCAC TTTTTCAAGT TGATAACGGA CTAGCCTTAT
     1141 TTTAACTTGC TATGCTGTTT TGAATGGTTC CAACAAGATT ATTTTATAAC TTTTATAACA
     1201 AATAATCAAG GAGAAATTCA AAGAAATTTA TCAGCCATAA AACAATACTT AATACTATAG
     1261 AATGATAACA AAATAAACTA CTTTTTAAAA GAATTTTGTG TTATAATCTA TTTATTATTA
     1321 AGTATTGGGT AATATTTTTT GAAGAGATAT TTTGAAAAAG AAAAATTAAA GCATATTAAA
     1381 CTAATTTCGG AGGTCATTAA AACTATTATT GAAATCATCA AACTCATTAT GGATTTAATT
     1441 TAAACTTTTT ATTTTAGGAG GCAAAAATGG ATAAGAAATA CTCAATAGGC TTAGcTATCG
     1501 GCACAAATAG CGTCGGATGG GCGGTGATCA CTGATGAATA TAAGGTTCCG TCTAAAAAGT
     1561 TCAAGGTTCT GGGAAATACA GACCGCCACA GTATCAAAAA AAATCTTATA GGGGCTCTTT
     1621 TATTTGACAG TGGAGAGACA GCGGAACGTA CTCGTCTCAA ACGGACAGCT CGTAGAAGGT
     1681 ATACACGTCG GAAGAATCGT ATTTGTTATC TACAGGAGAT TTTTTCAAAT GAGATGGCGA
     1741 AAGTAGATGA TAGTTTCTTT CATCGACTTG AAGAGTCTTT TTTGGTGGAA GAAGACAAGA
     1801 AGCATGAACG TCATCCTATT TTTGGAAATA TAGTAGATGA AGTTGCTTAT CATGAGAAAT
     1861 ATCCAACTAT CTATCATCTG CGAAAAAAAT TGGTAGATTC TACTGATAAA GCGGATTTGC
     1921 GCTTAATCTA TTTGGCCTTA GCGCATATGA TTAAGTTTCG TGGTCATTTT TTGATTGAGG
     1981 GAGATTTAAA TCCTGATAAT AGTGATGTGG ACAAACTATT TATCCAGTTG GTACAAACCT
     2041 ACAATCAATT ATTTGAAGAA AACCCTATTA ACGCAAGTGG AGTAGATGCT AAAGCGATTC
     2101 TTTCTGCACG ATTGAGTAAA TCAAGACGAT TAGAAAATCT CATTGCTCAG CTCCCCGGTG
     2161 AGAAGAAAAA TGGCTTATTT GGGAATCTCA TTGCTTTGTC ATTGGGTTTG ACCCCTAATT
     2221 TTAAATCAAA TTTTGATTTG GCAGAAGATG CTAAATTACA GCTTTCAAAA GATACTTACG
     2281 ATGATGATTT AGATAATTTA TTGGCGCAAA TTGGAGATCA ATATGCTGAT TTGTTTTTGG
     2341 CAGCTAAGAA TTTATCAGAT GCTATTTTAC TTTCAGATAT CCTAAGAGTA AATACTGAAA
     2401 TAACTAAGGC TCCCCTATCA GCTTCAATGA TTAAACGCTA CGATGAACAT CATCAAGACT
     2461 TGACTCTTTT AAAAGCTTTA GTTCGACAAC AACTTCCAGA AAAGTATAAA GAAATCTTTT
     2521 TTGATCAATC AAAAAACGGA TATGCAGGTT ATATTGATGG GGGAGCTAGC CAAGAAGAAT
     2581 TTTATAAATT TATCAAACCA ATTTTAGAAA AAATGGATGG TACTGAGGAA TTATTGGTGA
     2641 AACTAAATCG TGAAGATTTG CTGCGCAAGC AACGGACCTT TGACAACGGC TCTATTCCCC
     2701 ATCAAATTCA CTTGGGTGAG CTGCATGCTA TTTTGAGAAG ACAAGAAGAC TTTTATCCAT
     2761 TTTTAAAAGA CAATCGTGAG AAGATTGAAA AAATCTTGAC TTTTCGAATT CCTTATTATG
     2821 TTGGTCCATT GGCGCGTGGC AATAGTCGTT TTGCATGGAT GACTCGGAAG TCTGAAGAAA
     2881 CAATTACCCC ATGGAATTTT GAAGAAGTTG TCGATAAAGG TGCTTCAGCT CAATCATTTA
     2941 TTGAACGCAT GACAAACTTT GATAAAAATC TTCCAAATGA AAAAGTACTA CCAAAACATA
     3001 GTTTGCTTTA TGAGTATTTT ACGGTTTATA ACGAATTGAC AAAGGTCAAA TATGTTACTG
     3061 AAGGAATGCG AAAACCAGCA TTTCTTTCAG GTGAACAGAA GAAAGCCATT GTTGATTTAC
     3121 TCTTCAAAAC AAATCGAAAA GTAACCGTTA AGCAATTAAA AGAAGATTAT TTCAAAAAAA
     3181 TAGAATGTTT TGATAGTGTT GAAATTTCAG GAGTTGAAGA TAGATTTAAT GCTTCATTAG
     3241 GTACCTACCA TGATTTGCTA AAAATTATTA AAGATAAAGA TTTTTTGGAT AATGAAGAAA
     3301 ATGAAGATAT CTTAGAGGAT ATTGTTTTAA CATTGACCTT ATTTGAAGAT AGGGAGATGA
     3361 TTGAGGAAAG ACTTAAAACA TATGCTCACC TCTTTGATGA TAAGGTGATG AAACAGCTTA
     3421 AACGTCGCCG TTATACTGGT TGGGGACGTT TGTCTCGAAA ATTGATTAAT GGTATTAGGG
     3481 ATAAGCAATC TGGCAAAACA ATATTAGATT TTTTGAAATC AGATGGTTTT GCCAATCGCA
     3541 ATTTTATGCA GCTGATCCAT GATGATAGTT TGACATTTAA AGAAGACATT CAAAAAGCAC
     3601 AAGTGTCTGG ACAAGGCGAT AGTTTACATG AACATATTGC AAATTTAGCT GGTAGCCCTG
     3661 CTATTAAAAA AGGTATTTTA CAGACTGTAA AAGTTGTTGA TGAATTGGTC AAAGTAATGG
     3721 GGCGGCATAA GCCAGAAAAT ATCGTTATTG AAATGGCACG TGAAAATCAG ACAACTCAAA
     3781 AGGGCCAGAA AAATTCGCGA GAGCGTATGA AACGAATCGA AGAAGGTATC AAAGAATTAG
     3841 GAAGTCAGAT TCTTAAAGAG CATCCTGTTG AAAATACTCA ATTGCAAAAT GAAAAGCTCT
     3901 ATCTCTATTA TCTCCAAAAT GGAAGAGACA TGTATGTGGA CCAAGAATTA GATATTAATC
     3961 GTTTAAGTGA TTATGATGTC GATGCGATTG TTCCACAAAG TTTCCTTAAA GACGATTCAA
     4021 TAGACAATAA GGTCTTAACG CGTTCTGATA AAAATCGTGG TAAATCGGAT AACGTTCCAA
     4081 GTGAAGAAGT AGTCAAAAAG ATGAAAAACT ATTGGAGACA ACTTCTAAAC GCCAAGTTAA
     4141 TCACTCAACG TAAGTTTGAT AATTTAACGA AAGCTGAACG TGGAGGTTTG AGTGAACTTG
     4201 ATAAAGCTGG TTTTATCAAA CGCCAATTGG TTGAAACTCG CCAAATCACT AAGCATGTGG
     4261 CACAAATTTT GGATAGTCGC ATGAATACTA AATACGATGA AAATGATAAA CTTATTCGAG
     4321 AGGTTAAAGT GATTACCTTA AAATCTAAAT TAGTTTCTGA CTTCCGAAAA GATTTCCAAT
     4381 TCTATAAAGT ACGTGAGATT AACAATTACC ATCATGCCCA TGATGCGTAT CTAAATGCCG
     4441 TCGTTGGAAC TGCTTTGATT AAGAAATATC CAAAACTTGA ATCGGAGTTT GTCTATGGTG
     4501 ATTATAAAGT TTATGATGTT CGTAAAATGA TTGCTAAGTC TGAGCAAGAA ATAGGCAAAG
     4561 CAACCGCAAA ATATTTCTTT TACTCTAATA TCATGAACTT CTTCAAAACA GAAATTACAC
     4621 TTGCAAATGG AGAGATTCGC AAACGCCCTC TAATCGAAAC TAATGGGGAA ACTGGAGAAA
     4681 TTGTCTGGGA TAAAGGGCGA GATTTTGCCA CAGTGCGCAA AGTATTGTCC ATGCCCCAAG
     4741 TCAATATTGT CAAGAAAACA GAAGTACAGA CAGGCGGATT CTCCAAGGAG TCAATTCGTC
     4801 CAAAAAGAAA TTCGGACAAG CTTATTGCTC GTAAAAAAGA CTGGGATCCA AAAAAATATG
     4861 GTGGTTTTCT GTGGCCAACG GTAGCTTATT CAGTCCTAGT GGTTGCTAAG GTGGAAAAAG
     4921 GGAAATCGAA GAAGTTAAAA TCCGTTAAAG AGTTACTAGG GATCACAATT ATGGAAAGAA
     4981 GTTCCTTTGA AAAAAATCCG ATTGACTTTT TAGAAGCTAA AGGATATAAG GAAGTTAAAA
     5041 AAGACTTAAT CATTAAACTA CCTAAATATA GTCTTTTTGA GTTAGAAAAC GGTCGTAAAC
     5101 GGATGCTGGC TAGTGCCAAA CAGTTACAAA AAGGAAATGA GCTGGCTCTG CCAAGCAAAT
     5161 ATGTGAATTT TTTATATTTA GCTAGTCATT ATGAAAAGTT GAAGGGTAGT CCAGAAGATA
     5221 ACGAACAAAA ACAATTGTTT GTGGAGCAGC ATAAGCATTA TTTAGATGAG ATTATTGAGC
     5281 AAATCAGTGA ATTTTCTAAG CGTGTTATTT TAGCAGATGC CAATTTAGAT AAAGTTCTTA
     5341 GTGCATATAA CAAACATAGA GACAAACCAA TACGTGAACA AGCAGAAAAT ATTATTCATT
     5401 TATTTACGTT GACGCGTCTT GGAGCTCCCC GTGCTTTTAA ATATTTTGAT ACAACAATTG
     5461 ATCCGAAACA GTATCGTTCT ACAAAAGAAG TTTTAGATGC CACTCTTATC CATCAATCCA
     5521 TCACTGGTCT TTATGAAACA CGCATTGATT TGAGTCAGCT AGGAGGTGAC TAACtcgagt
     5581 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     5641 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     5701 gcctttctgc gtttataCCT AGagcgcccg gaagagagtc aattcagggt ggtgaatctg
     5761 cagtttacgg ctagctcagc cctaggtatt atgctagcGA ATTCATTAAA GAGGAGAAAG
     5821 GTACCatggg gcccgcttct aactttactc agttcgttct cgtcgacaat ggcggaactg
     5881 gcgacgtgac tgtcgcccca agcaacttcg ctaacgggat cgctgaatgg atcagctcta
     5941 actcgcgttc acaggcttac aaagtaacct gtagcgttcg tcagagctct gcgcagaatc
     6001 gcaaatacac catcaaagtc gaggtgccta aaggcgcctg gcgttcgtac ttaaatatgg
     6061 aactaaccat tccaattttc gccacgaatt ccgactgcga gcttattgtt aaggcaatgc
     6121 aaggtctcct aaaagatgga aacccgattc cctcagcaat cgcagcaaac tccggcatct
     6181 acGGTGGCGG AGGTAGCATG TCCCATCAGA AAATTATTCA GGATCTTATC GCATGGATTG
     6241 ACGAGCATAT TGACCAGCCG CTTAACATTG ATGTAGTCGC AAAAAAATCA GGCTATTCAA
     6301 AGTGGTACTT GCAACGAATG TTCCGCACGG TGACGCATCA GACGCTTGGC GATTACATTC
     6361 GCCAACGCCG CCTGTTACTG GCCGCCGTTG AGTTGCGCAC CACCGAGCGT CCGATTTTTG
     6421 ATATCGCAAT GGACCTGGGT TATGTCTCGC AGCAGACCTT CTCCCGCGTT TTCGCGCGGC
     6481 AGTTTGATCG CACTCCCGCG GATTATCGCC ACCGCCTGTA AGCGGCCGCc acgcaaaaaa
     6541 ccccgcttcg gcggggtttt ttcgccctag ggatatattc cgcttcctcg ctcactgact
     6601 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     6661 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     6721 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     6781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
     6841 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
     6901 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     6961 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7021 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
     7081 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
     7141 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
     7201 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
     7261 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
     7321 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtta
//