LOCUS       pCK760                  5540 bp ds-DNA     circular     30-JAN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(1..2229)
                     /label="pSC101** ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      2230..2335
                     /label="T0"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2365..3194)
                     /label="Amp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(3195..3220)
                     /label="Amp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3296..3321
                     /label="rbsR homology fw"
                     /note="sequence: TTAGAAAAATAAACAAATAGGGGTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3296..3326
                     /label="oCK1051"
                     /note="sequence: TTAGAAAAATAAACAAATAGGGGTTCCGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3324..3362)
                     /label="oBT24_new oBT22R"
                     /note="sequence: CGGAATTGCCAcaggtggcacttttcggggaaatgtgcg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3348..3387
                     /label="oCD584"
                     /note="sequence: cctgTGGCAATTCCGACGTCAGCATTTGCGATCATTCACG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3368..3391
                     /label="oCK384"
                     /note="sequence: cagcgcatcacgcgcATGCATAGCATTTGCGATCATTCACGCAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3471..3506)
                     /label="oJF366"
                     /note="sequence: GCCTTTGGTAACCGCAGGAGAAGTGAGGAGACGAGC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3492..3511)
                     /label="J106"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3492..3529
                     /label="oJF365"
                     /note="sequence: GCGGTTACCAAAGGCGTCCTCGTCGTCTTGAAGTTGCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3538..3572
                     /label="BBa_J23110"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3538..3572
                     /label="oBT107_J23110F"
                     /note="sequence: tttacggctagctcagtcctaggtacaatgctagc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3538..3572)
                     /label="oBT108_J23110R"
                     /note="sequence: gctagcattgtacctaggactgagctagccgtaaa"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          3551..3590
                     /label="oBT070_J23114F"
                     /note="sequence: CAGTCCTAGGTACAATGCTAGCGAATTCATTAAAGAGGAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3573..3599
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     primer          3573..3599
                     /label="oCD292"
                     /note="sequence: GAATTCATTAAAGAGGAGAAAGGTACC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3573..3599)
                     /label="oCDP004"
                     /note="sequence: GGTACCTTTCTCCTCTTTAATGAATTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     RBS             3582..3593
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     primer          3600..3624
                     /label="oCD282"
                     /note="sequence: ATGAGCAAAGGAGAAGAACTTTTCA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3600..4316
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3638..3657)
                     /label="jfgfp1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3654..3675)
                     /label="oCD032"
                     /note="sequence: TGTGCCCATTAACATCACCATC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4265..4284
                     /label="oCDP016"
                     /note="sequence: GTTTGTAACTGCTGCTGGGA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4294..4316)
                     /label="oJF258"
                     /note="sequence: TTATTTGTAGAGCTCATCCATGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4294..4316)
                     /label="oJF160"
                     /note="sequence: TTATTTGTAGAGCTCATCCATGC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      4341..4469
                     /label="dblTerm"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     primer          complement(4446..4475)
                     /label="oCK374"
                     /note="sequence: cgggggaatacaccttAGTACTtataaacgcagaaaggcccacccg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(4446..4491)
                     /label="oCK383"
                     /note="sequence: ATGATCGCAAATGCTgAGTACTtataaacgcagaaaggcccacccg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4470..4475
                     /label="ScaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          4475..4495
                     /label="oJF390"
                     /note="sequence: AAAACATATGGAATTCAGCATTTGCGATCATTCAC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4477..4500
                     /label="oCK1005"
                     /note="sequence: AGCATTTGCGATCATTCACGCAGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4477..4646
                     /label="J3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4582..4601
                     /label="J309"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(4591..4610)
                     /label="J308"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4592..4611
                     /label="J307"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(4601..4620)
                     /label="J306"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        4647..4681
                     /label="BBa_J23117"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4662..4681)
                     /label="oJF175"
                     /note="sequence: gctagcacaatccctaggac"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4682..4708
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     primer          complement(4684..4708)
                     /label="oJF158cd"
                     /note="sequence: GGTACCTTTCTCCTCTTTAATGAAT"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     RBS             4691..4702
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     misc_feature    4712..5386
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff3737"
                     /ApEinfo_fwdcolor="#ff3737"
     misc_feature    complement(4833..4852)
                     /label="RR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(4897..4916)
                     /label="RR2"
                     /ApEinfo_revcolor="#e0413b"
                     /ApEinfo_fwdcolor="#e0413b"
     primer          5411..5435
                     /label="oCD018"
                     /note="sequence: CCAGGCATCAAATAAAACGAAAGGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5411..5539
                     /label="dbl term"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(5521..5540)
                     /label="oJF391"
                     /note="sequence: CGCACTGCAGGAATTGTATAAACGCAGAAAGGCCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca
       61 gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct tccagaattg
      121 ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc
      181 gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg
      241 tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt
      301 aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa
      361 tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt
      421 gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt
      481 tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt
      541 tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat
      601 tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa
      661 tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg
      721 atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg
      781 ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc
      841 ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt
      901 tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga
      961 acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt
     1021 cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt
     1081 ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa
     1141 aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa
     1201 agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt
     1261 tagctaatac accataagca ttttccctac tgatgttcat catctgagcg tattggttat
     1321 aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg
     1381 ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta
     1441 gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt
     1501 aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt ttcccgggtg
     1561 atctgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac
     1621 cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa
     1681 tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata
     1741 actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc
     1801 ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca
     1861 aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt
     1921 gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg
     1981 cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc
     2041 ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag
     2101 cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt
     2161 cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact
     2221 gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2281 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2341 cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gTGGCAATTC
     3361 CGacgtcAGC ATTTGCGATC ATTCACGCAG CGCTTATTCA GTTGCTCACT GCGATGTCAT
     3421 AATCATCGCT ACGAGCTGTG AAAGATGCAT AAAGCTCGTA CGACGCGTTC GCTCGTCTCC
     3481 TCACTTCTCC TGCGGTTACC AAAGGCGTCC TCGTCGTCTT GAAGTTGCGA TTATAGAttt
     3541 acggctagct cagtcctagg tacaatgcta gcGAATTCAT TAAAGAGGAG AAAGGTACCA
     3601 TGAGCAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG
     3661 ATGTTAATGG GCACAAATTT TCTGTCCGTG GAGAGGGTGA AGGTGATGCT ACAAACGGAA
     3721 AACTCACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG CCAACACTTG
     3781 TCACTACTCT GACCTATGGT GTTCAATGCT TTTCCCGTTA TCCGGATCAC ATGAAACGGC
     3841 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAACGCACT ATATCTTTCA
     3901 AAGATGACGG GACCTACAAG ACGCGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA
     3961 ATCGTATCGA GTTAAAGGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAC
     4021 TCGAGTACAA CTTTAACTCA CACAATGTAT ACATCACGGC AGACAAACAA AAGAATGGAA
     4081 TCAAAGCTAA CTTCAAAATT CGCCACAACG TTGAAGATGG TTCCGTTCAA CTAGCAGACC
     4141 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC
     4201 TGTCGACACA ATCTGTCCTT TCGAAAGATC CCAACGAAAA GCGTGACCAC ATGGTCCTTC
     4261 TTGAGTTTGT AACTGCTGCT GGGATTACAC ATGGCATGGA TGAGCTCTAC AAAtaaggat
     4321 ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
     4381 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     4441 accttcgggt gggcctttct gcgtttataA GTACTcAGCA TTTGCGATCA TTCACGCAGC
     4501 GCTTATTCAG TTGCTCACTG CGATGTCATA ATCATCGCTA CGAGCTGTGA AAGATGCATA
     4561 AAGCTCGTAC GACGCGTTCG CTCGTCTCCT CACTTCTCCT ACGGAGCGTT CTGGACACAA
     4621 CGTCGTCTTG AAGTTGCGAT TATAGAttga cagctagctc agtcctaggg attgtgctag
     4681 cGAATTCATT AAAGAGGAGA AAGGTACCAT GGCGAGTAGC GAAGACGTTA TCAAAGAGTT
     4741 CATgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg
     4801 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg
     4861 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc
     4921 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt
     4981 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc
     5041 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc
     5101 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta
     5161 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg
     5221 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg
     5281 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
     5341 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaaggat ccaaactcga
     5401 gtaaggatct CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT
     5461 TTTATCTGTT GTTTGTCGGT GAACGCTCTC TACTAGAGTC ACACTGGCTC ACCTTCGGGT
     5521 GGGCCTTTCT GCGTTTATAC
//