LOCUS       pCK411_RR1              2268 bp ds-DNA     circular     29-SEP-2022
DEFINITION  .
ACCESSION   AY599232
VERSION     AY599232.2
KEYWORDS    "Parental Vector" "Origin" "Resistance" "Construction Method"
            "Promoter" "Terminator" "gRNA" "P.putida plasmids" "pCDP"
SOURCE      Cloning vector pUC18T-mini-Tn7T-Gm
  ORGANISM  Cloning vector pUC18T-mini-Tn7T-Gm other sequences; artificial
            sequences; vectors.
FEATURES             Location/Qualifiers
     misc_feature    complement(411..439)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          454..473
                     /label="oCDB021"
                     /note="sequence: TAAACAAATAGGGGTTCCGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          542..562
                     /label="oCD044"
                     /note="sequence: aataggcgtatcacgaggcag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          625..646
                     /label="oJF153"
                     /note="sequence: ATAAGTTGTTACTAGATTGACAGCTAGCTCAGTCCTAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     promoter        625..659
                     /label="J23119 (SpeI) promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(629..659)
                     /label="oBT116_J23119R"
                     /note="sequence: ACTAGTATTATACCtaggactgagctagctg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          635..659
                     /label="oCK079"
                     /note="sequence: GCTCAGTCCTAGGTATAATACTAGT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          644..702
                     /label="oCK684"
                     /note="sequence: TAGGTATAATACTAGTaactttcagtttagcggtctGTTTTAGAGCTAGAAATAGCAAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          644..702
                     /label="oCK1298"
                     /note="sequence: TAGGTATAATACTAGTAATTCgctagcATTATACCTGTTTTAGAGCTAGAAATAGCAAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          644..702
                     /label="oCK1297"
                     /note="sequence: TAGGTATAATACTAGTAATTCgctagcactatacctGTTTTAGAGCTAGAAATAGCAAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          644..703
                     /label="oCD091"
                     /note="sequence: TAGGTATAATACTAGTTGGAACCGTACTGGAACTGCGTTTTAGAGCTAGAAATAGCAAGT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    660..679
                     /label="Qi_RR1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          680..703
                     /label="oCK380"
                     /note="sequence: TAGGTATAATACTAGTtaagctttggaaccgtactgGTTTTAGAGCTAGAAATAGCAAGT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          680..703
                     /label="oCK379"
                     /note="sequence: TAGGTATAATACTAGTctttggaaccgtactggaacGTTTTAGAGCTAGAAATAGCAAGT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    680..721
                     /label="JAD designed chimera RNA 3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    726..762
                     /label="Streptococcus pyogenes tracrRNA terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(741..777)
                     /label="oBT185_sgRNA_R"
                     /note="sequence: GCTTTctcatgcgttAAAAAAAGCACCGACTCGGTGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          763..786
                     /label="oBT127_SynthT_F"
                     /note="sequence: aacgcatgagAAAGCCCCCGGAAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      763..815
                     /label="ECK120033736 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(791..815)
                     /label="oCK434"
                     /note="sequence: gtctGGTCTCgtgctAGTTGCATCGTACGTCGGTGgcgcaataaaAAAGCCCCCGGAAGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(791..835)
                     /label="oCK457"
                     /note="sequence: CTAGACTCGAGTAAGGATCCgcgcaataaaAAAGCCCCCGGAAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(791..841)
                     /label="oCK547"
                     /note="sequence: gggaacaaaagctggTCTAGCTTAAGgcgcaataaaAAAGCCCCCGGAAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(800..825)
                     /label="oBT148_Term_ins_R 143int"
                     /note="sequence: caggtggcacttttcggGTAAGGATCCgcgcaataaaAAAGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          817..863
                     /label="oCK683"
                     /note="sequence: TTAAGCTAGAccagcttttgttcccgcttcctcgctcactgactcgc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(983..999)
                     /label="oJF152"
                     /note="sequence: GAATCCAAGCACTAGTTTTTTACGGTTCCTGGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(1000..1682)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1016..1035)
                     /label="oCK216"
                     /note="sequence: gggcggagcctatggaaaaa"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1610..1633)
                     /label="oCK476"
                     /note="sequence: ccccgtagaaaagatcaaaggatc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(1780..171)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
       61 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      481 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      541 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
      601 tgatttctgg AATTCTAAAG ATCTTTGACA GCTAGCTCAG TCCTAGGTAT AATACTAGTa
      661 actttcagtt tagcggtctG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
      721 GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTaacgcatg agAAAGCCCC
      781 CGGAAGATCA CCTTCCGGGG GCTTTtttat tgcgcCTTAA GCTAGAccag cttttgttcc
      841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1441 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggc
//