LOCUS pSems-leader-SNA 8186 bp DNA circular SYN 30-SEP-2022 DEFINITION pSems leader fSNAP mXFPe1 IFNAR1 rotated to position 6000. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8186) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 30, 2022 from SnapGene Viewer 6.1.1 https://www.snapgene.com COMMENT Vector molecule: backbone cut AscI BamHI Fragment ends: BamHI and AscI Fragment size: 7461 Insert molecule: PCR product cut AscI BamHI Fragment ends: AscI and BamHI Fragment size: 725 FEATURES Location/Qualifiers source 1..8186 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 804..866 /codon_start=1 /label=Ig k-chain leader sequence /translation="METDTLLLWVLLLWVPGSTGD" misc_feature 867..893 /label=Hemagglutinin A epitope CDS 903..1448 /codon_start=1 /label=SnapF /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA VKEWLLAHEGHRLGKPGLG" CDS 1470..2186 /codon_start=1 /label=mEGFP (N198D Y200F Y66F) /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTFGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDDHFLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2202..3791 /codon_start=1 /label=Ifnar1 /translation="KNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDN WIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPP EVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLLIWKNSSGVEERIENIYSRHKIY KLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWD YTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQ ASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPL IYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVC EKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQ PLKNLLLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDES ESKTSEELQQDFV" CDS 4759..5559 /codon_start=1 /label=Neomycin /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 61 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 121 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 301 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc 361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 721 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt 781 ggtaccgagc tcggatcgat atcatggaga cagacacact cctgctatgg gtactgctgc 841 tctgggttcc aggttccact ggtgactatc catatgatgt tccagattat gctgaattca 901 ccatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg ggcaagctgg 961 aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa ggaacatctg 1021 ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca gagccactga 1081 tgcaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc gaggagttcc 1141 ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc caggtgctgt 1201 ggaaactgct gaaagtggtg aagttcggag aggtcatcag ctacagccac ctggccgccc 1261 tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc cctgagcgga aatcccgtgc 1321 ccattctgat cccctgccac cgggtggtgc agggcgacct ggacgtgggg ggctacgagg 1381 gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg ggcaagcctg 1441 ggctgggtcc tgcaggtata ggcgcgccca tggtgagcaa gggcgaggag ctgttcaccg 1501 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 1561 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 1621 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgaccttt ggcgtgcagt 1681 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 1741 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 1801 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 1861 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 1921 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 1981 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 2041 acggccccgt gctgctgccc gacgatcact tcctgagcac ccagtccaaa ctgagcaaag 2101 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 2161 ctctcggcat ggacgagctg tacaagggat ccctcgagtc taaaaatcta aaatctcctc 2221 aaaaagtaga ggtcgacatc atagatgaca actttatcct gaggtggaac aggagcgatg 2281 agtctgtcgg gaatgtgact ttttcattcg attatcaaaa aactgggatg gataattgga 2341 taaaattgtc tgggtgtcag aatattacta gtaccaaatg caacttttct tcactcaagc 2401 tgaatgttta tgaagaaatt aaattgcgta taagagcaga aaaagaaaac acttcttcat 2461 ggtatgaggt tgactcattt acaccatttc gcaaagctca gattggtcct ccagaagtac 2521 atttagaagc tgaagataag gcaatagtga tacacatctc tcctggaaca aaagatagtg 2581 ttatgtgggc tttggatggt ttaagcttta catatagctt acttatctgg aaaaactctt 2641 caggtgtaga agaaaggatt gaaaatattt attccagaca taaaatttat aaactctcac 2701 cagagactac ttattgtcta aaagttaaag cagcactact tacgtcatgg aaaattggtg 2761 tctatagtcc agtacattgt ataaagacca cagttgaaaa tgaactacct ccaccagaaa 2821 atatagaagt cagtgtccaa aatcagaact atgttcttaa atgggattat acatatgcaa 2881 acatgacctt tcaagttcag tggctccacg cctttttaaa aaggaatcct ggaaaccatt 2941 tgtataaatg gaaacaaata cctgactgtg aaaatgtcaa aactacccag tgtgtctttc 3001 ctcaaaacgt tttccaaaaa ggaatttacc ttctccgcgt acaagcatct gatggaaata 3061 acacatcttt ttggtctgaa gagataaagt ttgatactga aatacaagct ttcctacttc 3121 ctccagtctt taacattaga tcccttagtg attcattcca tatctatatc ggtgctccaa 3181 aacagtctgg aaacacgcct gtgatccagg attatccact gatttatgaa attatttttt 3241 gggaaaacac ttcaaatgct gagagaaaaa ttatcgagaa aaaaactgat gttacagttc 3301 ctaatttgaa accactgact gtatattgtg tgaaagccag agcacacacc atggatgaaa 3361 agctgaataa aagcagtgtt tttagtgacg ctgtatgtga gaaaacaaaa ccaggaaata 3421 cctctaaaat ttggcttata gttggaattt gtattgcatt atttgctctc ccgtttgtca 3481 tttatgctgc gaaagtcttc ttgagatgca tcaattatgt cttctttcca tcacttaaac 3541 cttcttccag tatagatgag tatttctctg aacagccatt gaagaatctt ctgctttcaa 3601 cttctgagga acaaatcgaa aaatgtttca taattgaaaa tataagcaca attgctacag 3661 tagaagaaac taatcaaact gatgaagatc ataaaaaata cagttcccaa actagccaag 3721 attcaggaaa ttattctaat gaagatgaaa gcgaaagtaa aacaagtgaa gaactacagc 3781 aggactttgt atgagcggcc gcatagataa ctgatccagt gtgctggaat taattcgctg 3841 tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat gacttctgcg 3901 ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg 3961 cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt gtcaagcttg 4021 aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc cactttgcct 4081 ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca tctagggcgg 4141 ccaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata 4201 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 4261 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 4321 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 4381 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 4441 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 4501 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 4561 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 4621 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc 4681 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccggg 4741 ataattcctg cagccaatat gggatcggcc attgaacaag atggattgca cgcaggttct 4801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 4861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 4921 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 4981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 5041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 5101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 5161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 5221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 5281 gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc 5341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 5401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 5461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 5521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatca attctctaga 5581 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 5641 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 5701 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 5761 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc 5821 gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 5881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 5941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 6481 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 6541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 6601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 6661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 6721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 6781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 6841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 6961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 7141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 7201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 7261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 7321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 7381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 7501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 7561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 7621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 7681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 7741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 7801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 7861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 7921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 7981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 8101 atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 8161 tgcttgtgtg ttggaggtcg ctgagt //