LOCUS       pSems-leader-SNA        8186 bp DNA     circular SYN 30-SEP-2022
DEFINITION  pSems leader fSNAP mXFPe1 IFNAR1 rotated to position 6000.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2022 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
COMMENT     Vector molecule:  backbone cut AscI BamHI
               Fragment ends: BamHI and AscI
               Fragment size: 7461
            Insert molecule:  PCR product cut AscI BamHI
               Fragment ends: AscI and BamHI
               Fragment size: 725
FEATURES             Location/Qualifiers
     source          1..8186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             804..866
                     /codon_start=1
                     /label=Ig k-chain leader sequence
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    867..893
                     /label=Hemagglutinin A epitope
     CDS             903..1448
                     /codon_start=1
                     /label=SnapF
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             1470..2186
                     /codon_start=1
                     /label=mEGFP (N198D Y200F Y66F)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTFGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDDHFLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2202..3791
                     /codon_start=1
                     /label=Ifnar1
                     /translation="KNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDN
                     WIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPP
                     EVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLLIWKNSSGVEERIENIYSRHKIY
                     KLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWD
                     YTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQ
                     ASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPL
                     IYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVC
                     EKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQ
                     PLKNLLLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDES
                     ESKTSEELQQDFV"
     CDS             4759..5559
                     /codon_start=1
                     /label=Neomycin
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
       61 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      121 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      301 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      721 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      781 ggtaccgagc tcggatcgat atcatggaga cagacacact cctgctatgg gtactgctgc
      841 tctgggttcc aggttccact ggtgactatc catatgatgt tccagattat gctgaattca
      901 ccatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg ggcaagctgg
      961 aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa ggaacatctg
     1021 ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca gagccactga
     1081 tgcaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc gaggagttcc
     1141 ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc caggtgctgt
     1201 ggaaactgct gaaagtggtg aagttcggag aggtcatcag ctacagccac ctggccgccc
     1261 tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc cctgagcgga aatcccgtgc
     1321 ccattctgat cccctgccac cgggtggtgc agggcgacct ggacgtgggg ggctacgagg
     1381 gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg ggcaagcctg
     1441 ggctgggtcc tgcaggtata ggcgcgccca tggtgagcaa gggcgaggag ctgttcaccg
     1501 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1561 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1621 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgaccttt ggcgtgcagt
     1681 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1741 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1801 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1861 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1921 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1981 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2041 acggccccgt gctgctgccc gacgatcact tcctgagcac ccagtccaaa ctgagcaaag
     2101 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     2161 ctctcggcat ggacgagctg tacaagggat ccctcgagtc taaaaatcta aaatctcctc
     2221 aaaaagtaga ggtcgacatc atagatgaca actttatcct gaggtggaac aggagcgatg
     2281 agtctgtcgg gaatgtgact ttttcattcg attatcaaaa aactgggatg gataattgga
     2341 taaaattgtc tgggtgtcag aatattacta gtaccaaatg caacttttct tcactcaagc
     2401 tgaatgttta tgaagaaatt aaattgcgta taagagcaga aaaagaaaac acttcttcat
     2461 ggtatgaggt tgactcattt acaccatttc gcaaagctca gattggtcct ccagaagtac
     2521 atttagaagc tgaagataag gcaatagtga tacacatctc tcctggaaca aaagatagtg
     2581 ttatgtgggc tttggatggt ttaagcttta catatagctt acttatctgg aaaaactctt
     2641 caggtgtaga agaaaggatt gaaaatattt attccagaca taaaatttat aaactctcac
     2701 cagagactac ttattgtcta aaagttaaag cagcactact tacgtcatgg aaaattggtg
     2761 tctatagtcc agtacattgt ataaagacca cagttgaaaa tgaactacct ccaccagaaa
     2821 atatagaagt cagtgtccaa aatcagaact atgttcttaa atgggattat acatatgcaa
     2881 acatgacctt tcaagttcag tggctccacg cctttttaaa aaggaatcct ggaaaccatt
     2941 tgtataaatg gaaacaaata cctgactgtg aaaatgtcaa aactacccag tgtgtctttc
     3001 ctcaaaacgt tttccaaaaa ggaatttacc ttctccgcgt acaagcatct gatggaaata
     3061 acacatcttt ttggtctgaa gagataaagt ttgatactga aatacaagct ttcctacttc
     3121 ctccagtctt taacattaga tcccttagtg attcattcca tatctatatc ggtgctccaa
     3181 aacagtctgg aaacacgcct gtgatccagg attatccact gatttatgaa attatttttt
     3241 gggaaaacac ttcaaatgct gagagaaaaa ttatcgagaa aaaaactgat gttacagttc
     3301 ctaatttgaa accactgact gtatattgtg tgaaagccag agcacacacc atggatgaaa
     3361 agctgaataa aagcagtgtt tttagtgacg ctgtatgtga gaaaacaaaa ccaggaaata
     3421 cctctaaaat ttggcttata gttggaattt gtattgcatt atttgctctc ccgtttgtca
     3481 tttatgctgc gaaagtcttc ttgagatgca tcaattatgt cttctttcca tcacttaaac
     3541 cttcttccag tatagatgag tatttctctg aacagccatt gaagaatctt ctgctttcaa
     3601 cttctgagga acaaatcgaa aaatgtttca taattgaaaa tataagcaca attgctacag
     3661 tagaagaaac taatcaaact gatgaagatc ataaaaaata cagttcccaa actagccaag
     3721 attcaggaaa ttattctaat gaagatgaaa gcgaaagtaa aacaagtgaa gaactacagc
     3781 aggactttgt atgagcggcc gcatagataa ctgatccagt gtgctggaat taattcgctg
     3841 tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat gacttctgcg
     3901 ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg
     3961 cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt gtcaagcttg
     4021 aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc cactttgcct
     4081 ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca tctagggcgg
     4141 ccaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4201 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4261 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4321 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4381 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4441 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4501 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4561 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4621 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4681 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccggg
     4741 ataattcctg cagccaatat gggatcggcc attgaacaag atggattgca cgcaggttct
     4801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4921 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5281 gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc
     5341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatca attctctaga
     5581 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     5641 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     5701 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5761 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc
     5821 gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     5881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6481 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     6541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8101 atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8161 tgcttgtgtg ttggaggtcg ctgagt
//
