LOCUS       pMUT2-TcpP-mCherry      7311 bp ds-DNA     circular     04-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          36..55
                     /label="443_pHH seq primer"
                     /note="sequence: GGCGATTAAGTTGGGTAACG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          95..113
                     /label="444_pHH seq primer 2"
                     /note="sequence: AGTGAGCGCGACGTAATAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          95..118
                     /label="HH124"
                     /note="sequence: AGTGAGCGCGACGTAATACGACTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          155..198
                     /label="HH380"
                     /note="sequence: CGTGTCTTGTCCGCGGTACCTACTATAAACGCAGAAAGGCCCAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(158..177)
                     /label="HH378"
                     /note="sequence: GTAGGTACCGCGGACAAGAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(178..306)
                     /label="Terminator B_0015"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(178..306)
                     /label="Terminator B0015"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    178..1864
                     /label="gBlock "
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(307..717)
                     /label="TcpH"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(307..717)
                     /label="Translation 717-307"
     misc_feature    complement(718..745)
                     /label="Endogenous RBS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(746..781)
                     /label="P5 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="http://parts.igem.org/Part:BBa_K3702006 source. https://www.nature.com/articles/nmeth.2404#MOESM321"
     misc_feature    complement(782..894)
                     /label="T1 Terminator rnpB "
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     None            complement(895..1132)
                     /label="TcpP transmembrane domain and periplasmic domain"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(895..1132)
                     /label="Translation 1132-895"
     misc_feature    complement(926..937)
                     /label="Mutated ligand-binding domain"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1133..1597)
                     /label="CadC DNBD"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(1133..1597)
                     /label="Translation 1597-1133"
     misc_feature    complement(1598..1624)
                     /label="Endogenous RBS "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     None            complement(1625..1659)
                     /label="P9 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1632..1637)
                     /label="-10 region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1660..1725
                     /label="Non-interfering linker sequence "
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1726..1887
                     /label="pCadBA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1846..1887)
                     /label="HH379"
                     /note="sequence: CCAAACTGGCGATAAGATTACTCACGAAAAAAGGATTAATCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1870..1909
                     /label="HH410"
                     /note="sequence: ATCTTATCGCCAGTTTGGTCAGGAGAATTACAATGGTTTC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1878..1903)
                     /label="THV9"
                     /note="sequence: ATTGTAATTCTCCTGACCAAACTGGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1888..1896
                     /label="RBS (383062)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1902..2612
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1902..2612
                     /label="Translation 1902-2612"
     misc_feature    1916..1924
                     /label="Second RBS (1168899)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2536..2541)
                     /label="-10 region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2613..2710
                     /label="Backbone fragment"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      complement(2613..2710)
                     /label="terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Site            complement(2613..2710)
                     /label="annotated as hok/sok locus"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            complement(2714..2872)
                     /label="hok gene"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(2714..2872)
                     /label="hok CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2718..2930)
                     /label="mok CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            complement(2718..2930)
                     /label="mok gene"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2723..2748)
                     /label="HH125"
                     /note="sequence: TGGCGGCTTTCATGGCTTACGAATCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2730..2748)
                     /label=419
                     /note="sequence: TGGCGGCTTTCATGGCTTA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            2916..3140
                     /label="sok gene"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             2916..3140
                     /label="sok CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            complement(3152..3180)
                     /label="psiA gene"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(3152..3180)
                     /label="psiA CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3181..3203
                     /label=105
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3186..3203)
                     /label="532_hok removal"
                     /note="sequence: GGATAACAGGGCACGGCTGCATTGAGTGTCTCTTGTG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3192..3215)
                     /label=104
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3216..3233
                     /label=209
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3216..3239)
                     /label=212
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            3216..3248
                     /label="nikA / mobA / copG (plasmid mobility)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3232..3251)
                     /label="485_pVTSR rev dsbC"
                     /note="sequence: TAGTAGTGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAAGCTAATCTGCCATAAGCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3286..3307
                     /label="486_pVTSR fw dsbA"
                     /note="sequence: GAAATATCTGTCCGAGAAAAAAtaaCACCGTAAGCTCCCAAAGTG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(3288..3307)
                     /label=294
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(3638..4066)
                     /label="putative HTH domain protein (MarR-like)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(3749..4066)
                     /label="putative HTH domain protein"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3787..3792
                     /label="-35 region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     -10_signal      complement(4095..4103)
                     /label="potential -10 site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4102..4107)
                     /label="-10 region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Site            4111..4124
                     /label="Oswald 2006 entered here"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     -10_signal      complement(4114..4119)
                     /label="potential -35 site"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4223..4393
                     /label="uncharacterized protein"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             4357..4729
                     /label="hypothetical protein"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4466..4471
                     /label="-35 region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4661..4680
                     /label=237
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5079..5098)
                     /label=51
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5287..5307
                     /label=236
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_RNA        5306..5326
                     /label="gRNA target"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5564..5571
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(5620..5814)
                     /label="putative Replication RNAi related"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5620..5814)
                     /label="putative rom (RNA I inhibition modulator protein)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(5856..5861)
                     /label="-10 region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     oriT            5862..6047
                     /label="putative oriT"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     gene            6001..6047
                     /label="nikA / mobA / copG (plasmid mobility)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          6024..6068
                     /label=211
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(6029..6047)
                     /label=210
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            complement(6167..6857)
                     /label="KanR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6740..6745
                     /label="AgeI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(6858..6971)
                     /label="KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     regulatory      complement(6972..7041)
                     /label="Pbla; upstream region of the bla ORF includingpromoter and RBS; derived from pUC19"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          7005..7025
                     /label=92
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          7042..7074
                     /label="THV7"
                     /note="sequence: TGTATTTAGAAAAATAAACAAATAGGGGTTCCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG
       61 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGACGTAA TACGACTCAC
      121 TATAGGGCGA ATTGAAGGAA GGCCGTCAAG GCCACGTGTC TTGTCCGCGG TACCTACTAT
      181 AAACGCAGAA AGGCCCACCC GAAGGTGAGC CAGTGTGACT CTAGTAGAGA GCGTTCACCG
      241 ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT
      301 GCCTGGTTAG AAATCGCTCT GACACGAGAA CCACATCTTT TCCGCCGTCA GCAATTTGTA
      361 AGGAAAAGCC AGAAAGCACC CTTTGCCAAG TTGATAGATC GACAATACAT TGTAATCACG
      421 AGAAGCATTC GATTGGGAGT AATCGGGGAT CAGATTCAGC TTTTGTGATG AAGGGTCAGG
      481 CAATGTTTGG TAGCGTGTAG TCCATGATTT AACAACATTA CCTTGAGGGT CCGTCTGAGT
      541 AAGCCACACA TTAGACAGAT TGCCCTGGTA GGTGCTAAAG ATTTGAGTAA CGGTTCCATC
      601 AATAATCATG GTCCCGTGGC TATTTTTCTG GCGCATAGGA AGAGCAATAA TTGTTACCCC
      661 CAGGGCGACC ACAAACAGAC CAGTGGCCCC TCCCCAAGCC TTTAACTTCT TGTGCATTAC
      721 TTTACATTTT CTTAATAATA ACTACtccac ataaattacg agccggatga ttaattgtca
      781 atttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
      841 aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccggTTTATT
      901 AGTTCTTAGT ACATTCCAAC GTCTTATCAG AAAAAACACA TTGATGCTGG TCGATAGCAA
      961 AGGTCAACTT CTGCTCGCTA AGTTTCTGGA TTGTGATTCC AGGAAGACGG GCTAAGTCAC
     1021 GTAAATCGTG AGTAATCCCG GCCAATTCGT GTTGATACCA TTGCCCGTAT GACCACCAAA
     1081 TGACAGGTAA TAAGGCAACA TATAACGCTG AAAACACCAG ATACGGCACC ACTCGTTTGC
     1141 TTTTAACTGG GGATTGTTCA GGTGGCGTTG CGGTGTTTTG AATGTTAAGA CTGTGGGAGG
     1201 GAGAATCTGT GGCAGGAACC GCCTCTGGTA TAGGGGGAGG CGAAGATAGC ATTATTTCCT
     1261 CTCCCTCTTC TTCGCTGTAC CAGATAACCG GCACCATTAA TTTATAGCCG CGCTTTGGTA
     1321 CAGTAGCGAT ATAGACAGGA CTATCTTCAT CATTATCTTT TAATGACTTA CGTAGTTCTG
     1381 AGATACTCTG CGTCACAACG TGATTGGTGA CAATACTTCT CTTCCAGACA TTATCGATAA
     1441 GTTCATCCCT GCTAAGTACT TCGCCACTGT GTTGAGCAAA GAAAACCAGA AGATCGATTA
     1501 ATCTCGGCTC AAGGGTAAGT TGACGCCCAT TGCGGCTAAT TTGGTTTATG GACGGAGTAA
     1561 CAAGCCATTC GCCAACGCGA ACTACAGGTT GTTGCATATG TCTTCTGTTC ATAATTCACG
     1621 GAACTCCACA CATTATACGA GCCGATGATT AAGAGGCAAG AAATAAGATC ACTACCGGGC
     1681 GTATTTTTTG AGTTATCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATCCA TTGTAAACAT
     1741 TAAATGTTTA TCTTTTCATG ATATCAACTT GCGATCCTGA TGTGTTAATA AAAAACCTCA
     1801 AGTTCTCACT TACAGAAACT TTTGTGTTAT TTCACCTAAT CTTTAGGATT AATCCTTTTT
     1861 TCGTGAGTAA TCTTATCGCC AGTTTGGTCA GGAGAATTAC AATGGTTTCC AAGGGCGAGG
     1921 AGGATAACAT GGCTATCATT AAAGAGTTCA TGCGCTTCAA AGTTCACATG GAGGGTTCTG
     1981 TTAACGGTCA CGAGTTCGAG ATCGAAGGCG AAGGCGAGGG CCGTCCGTAT GAAGGCACCC
     2041 AGACCGCCAA ACTGAAAGTG ACTAAAGGCG GCCCGCTGCC TTTTGCGTGG GACATCCTGA
     2101 GCCCGCAATT TATGTACGGT TCTAAAGCGT ATGTTAAACA CCCAGCGGAT ATCCCGGACT
     2161 ATCTGAAGCT GTCTTTTCCG GAAGGTTTCA AGTGGGAACG CGTAATGAAT TTTGAAGATG
     2221 GTGGTGTCGT GACCGTCACT CAGGACTCCT CCCTGCAGGA TGGCGAGTTC ATCTATAAAG
     2281 TTAAACTGCG TGGTACTAAT TTTCCATCTG ATGGCCCGGT GATGCAGAAA AAGACGATGG
     2341 GTTGGGAGGC GTCTAGCGAA CGCATGTATC CGGAAGATGG TGCGCTGAAA GGCGAAATTA
     2401 AACAGCGCCT GAAACTGAAA GATGGCGGCC ATTATGACGC TGAAGTGAAA ACCACGTACA
     2461 AAGCCAAGAA ACCTGTGCAG CTGCCTGGCG CGTACAATGT GAATATTAAA CTGGACATCA
     2521 CCTCTCATAA TGAAGATTAT ACGATCGTAG AGCAATATGA GCGCGCGGAG GGTCGTCATT
     2581 CTACCGGTGG CATGGATGAG CTGTACAAAT AAAAAGGGGC TACCGGCGAA CCAGCAGCCC
     2641 CTTTATAAAG GCGCTTCAGT AGTCAGACCA GCATCAGTCC TGAAAAGGCG GGCCTGCGCC
     2701 CGCCTCCAGG TTGCTACTTA CCGGATTCGT AAGCCATGAA AGCCGCCACC TCCCTGTGTC
     2761 CGTCTCTGTA ACGAATCTCG CACAGCGATT TTCGTGTCAG ATAAGTGAAT ATCAACAGTG
     2821 TGAGACACAC GATCAACACA CACCAGACAA GGGAACTTCG TGGTAGTTTC ATGGCCTTCT
     2881 TCTCCTTGCG CAAAGCGCGG TAAGAGGCTA TCCTGATGTG GACTAGACAT AGGGATGCCT
     2941 CGTGGTGGTT AATGAAAATT AACTTACTAC GGGGCTATCT TCTTTCTGCC ACACAACACG
     3001 GCAACAAACC ACCTTCACGT CATGAGGCAG AAAGCCTCAA GCGCCGGGCA CATCATAGCC
     3061 CATATACCTG CACGCTGACC ACACTCACTT TCCCTGAAAA TAATCCGCTC ATTCAGACCG
     3121 TTCACGGGAA ATCCGTGTGA TTGTTGCCGC ATCACGCTGC CTCCCGGAGT TTGTTCGGCA
     3181 TAAGTCACAA GAGACACTCA ATGGCAAAGG AAGACCGTGC CCTGTTATCC AGGCTTATGG
     3241 CAGATTAGCT TCCCGGAGAG AAAACTGTCG AAAACTGACG GTATGAACAC CGTAAGCTCC
     3301 CAAAGTGATC ACCATTCGCT TTCATGCATA GCTATGCAGC GAGCTGAAAC GATCCTGACG
     3361 CATCCTTCCT GTTTTTCCGG GGTAAAACAT CTCTTTTTGC GGTGTCTCGC GTCAGAATCG
     3421 CGTTCAGCGC GTTTCAGTGG TGCGTACAAT TAAGGGATTA TGGTAAATAT ATGAGCTATG
     3481 CGATAACTTT AACTGTGAAG CGATGAACCC ATTACAGGCA AAGCCAATTA CTCCTGACAG
     3541 TGGTTTAGCC AGAAGCAGGG CTACCAAGAC CAATGCAATA AGTAATATAT CGTTTTGCTA
     3601 TCGTGCCATC CGTCGCGCTC AGTTCCATTG TGCTTTTTTA AGCTGTCGTT TTTCTTACGG
     3661 TATATACCGG TTTTTTATGG CGTGGTTTCT TAACTTGTTC AGCTACTGAT GGACCCATGT
     3721 ATCTAGGTAG TCAACTAGCT TTGTTAGATC ATAAAATATT GCGACCACCA TATCGGCGAT
     3781 CACTCTTCGA TGTTGGTTTC TTGTCCAAGA GATTAGCTTT TTCAAGATCA TTGATAGCTC
     3841 TCTGAACAGT CCGTACAGAA ACCCCCATAC GTATGGCTAG ACTTTCCATT GACGGATGCG
     3901 GCCACTCTTG CAAACTCCAC CAGTGAACGA TCAGGTTAAG TAGTGTGTTA AAGGCCACTG
     3961 AAGTTAGCTT TTTCTCGTTT TGTATAAAAA ACAATACGGT AGGCACTGCT GTCCAGCCAA
     4021 GAGACAAACC GCCAGCTTTC CATTTATTCT TAACGGAGTA AGTCATTGAT TTTCCTAAGC
     4081 CCCAAAATAT TTAAAGTATA TATTATATGT ATATTCATAT GAATAGGGTG ACACTGGCGC
     4141 CATTATTGTG CAACCAAAAA AGACTACTCT GAAAACGAGG AAAAGATTTT TTCCTGCCTG
     4201 AATTAGATAC GGAGTTAGCG ATATGAAAAC CGAACAACGT CATGATCTTG TTAAAGATAT
     4261 TGAGGTTTTT GGCGTATCCT TGTCTCTGTT GATTTCCAGA GCGAATGAGA AGTCTGTTAC
     4321 AATGCCATCT GGTCTAAGTC GGGAGCAGAG AAGAGCATGG GCAGCGGAGC AGGCGCGCAA
     4381 AATCCACAAT TGAATATTGT CTCATTCTCT GAGACCTTCA ACCTTTATTA CACATCCAGA
     4441 TATTCTGCAA AAACACTCGA TAAAATCGAT GATTTCATTG AGCATTTTGA AAAATACAAT
     4501 CTCTTTGGCG ATCCTTTAAA AGGATATCCA GCTTGGACTG GCAAAGTATC GCCATCGTGG
     4561 AAAGTGCCTG ATCATTACGA AAACAAAGAA GCTATTGAGA AGTATGCTAG AGCTAACAAA
     4621 TTATGGCATG CTCATTTAGG CGATCCGGTT TTTAAAGATA CGTTTCATGG GAAATACAAG
     4681 GTTTCTGACT GGGTTATTCA TTTCCAGCGG CTGACACCGA ACCATATAAA GCTTTTAGAG
     4741 CTTGGATACC ATGACCCAAT GAAGCTACCT CAAAACTTTG AATGATCGAG CGGCAGGCTA
     4801 AATGAAATCT TGAGATTCAT TCAGTCTCGT CGTAATCTCA CTATTGTAAA AACGAAAAAA
     4861 CCACCCTGGC AGGTGGTTTT TCGAAGGTTA GTTAATCCTG GCAGATTCTC TAACCGTGGT
     4921 AACAGTCTTG TGCGAGACAT GTCACCAAAT ACTGTCCTTT CAGTGTAGCC TCAGTTAGGC
     4981 CGCCACTTCA AGAACTCTCG TTACATCTCT CGCACATCCT GCTTACCAGT GGCCGTTGCC
     5041 AGTGGCGTTA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     5101 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     5161 ACCGAACCGA GATACCAACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     5221 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     5281 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     5341 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CTCCCGCGCG
     5401 CCCTTCTTCT GGGTCTTTGT CTTTTGCTCA CATGTTCTTT CCGGTTTTAT CCCCCGATTC
     5461 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGACACC GCTCGCCGCA GTCGAACGAC
     5521 CGAGCGTAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTTATGTGAC ATTTTCTCCT
     5581 TACGCTCCTC TATGCCGTTC TGCATCCTGT CCGGATGCGT TATATCCCGG TAAGATTTTC
     5641 CGCTTCAAAG CGTGTCTGTA TGCTGTTCTG GAGTTCTTCT GCGAGTTCGT GCAGTTTCTC
     5701 ACACATGGCG GCCTGTTCGT CGGCATTGAG TGCGTCCAGT TTTTCGAGCA GCGTCAGGCT
     5761 CTGACTTTTT ATGAATCCCG CCATGTTGAG TACGGCTTGC TGCTGCTTAT TCATCTTTTC
     5821 GTTTTCTCCG TTCTGTCTGT CATCTGCGTT GTGTGATTAT ATCGCGTACC ACTTTTCGAC
     5881 TGTTTTGCTG CCGCTATTCT GCCGCTTGGC TTTTTGACGG GCATTTCTGT CAGACAACAC
     5941 TGTCACTGCC AAAAAACTGC CGTGCCTTTG TCGGTAATTC GAGCTTGCTG ACAGGACAGG
     6001 ATGTGCAATT GTTATACCGC GCATACATGC ACGCTATTAC AATTGCCCGT TAAGGGATTT
     6061 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
     6121 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTATTAG AAAAATTCAT
     6181 CCAGCAGACG ATAAAACGCA ATACGCTGGC TATCCGGTGC CGCAATGCCA TACAGCACCA
     6241 GAAAACGATC CGCCCATTCG CCGCCCAGTT CTTCCGCAAT ATCACGGGTG GCCAGCGCAA
     6301 TATCCTGATA ACGATCCGCC ACGCCCAGAC GGCCGCAATC AATAAAGCCG CTAAAACGGC
     6361 CATTTTCCAC CATAATGTTC GGCAGGCACG CATCACCATG GGTCACCACC AGATCTTCGC
     6421 CATCCGGCAT GCTCGCTTTC AGACGCGCAA ACAGCTCTGC CGGTGCCAGG CCCTGATGTT
     6481 CTTCATCCAG ATCATCCTGA TCCACCAGGC CCGCTTCCAT ACGGGTACGC GCACGTTCAA
     6541 TACGATGTTT CGCCTGATGA TCAAACGGAC AGGTCGCCGG GTCCAGGGTA TGCAGACGAC
     6601 GCATGGCATC CGCCATAATG CTCACTTTTT CTGCCGGCGC CAGATGGCTA GACAGCAGAT
     6661 CCTGACCCGG CACTTCGCCC AGCAGCAGCC AATCACGGCC CGCTTCGGTC ACCACATCCA
     6721 GCACCGCCGC ACACGGAACA CCGGTGGTGG CCAGagcact actaCCAGCT CAGACGCGCC
     6781 GCTTCATCCT GCAGCTCGTT CAGCGCACCG CTCAGATCGG TTTTCACAAA CAGCACCGGA
     6841 CGACCCTGCG CGCTCAGACG AAACACCGCC GCATCAGAGC AGCCAATGGT CTGCTGCGCC
     6901 CAATCATAGC CAAACAGACG TTCCACCCAC GCTGCCGGGC TACCCGCATG CAGGCCATCC
     6961 TGTTCAATCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     7021 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     7081 TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA
     7141 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA
     7201 AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGGCCGCTA CAGGGCGCTC CCATTCGCCA
     7261 TTCAGGCTGC GCAACTGTTG GGAAGGGCGT TTCGGTGCGG GCCTCTTCGC T
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