LOCUS       AV0.1-CWS-Cre-tdT        6987 bp    DNA     circular      3-DEC-2019
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13106555|
COMMENT     VNTDBDATE|-13106555|
COMMENT     LSOWNER|
COMMENT     VNTNAME|AV0.1-CWS-Cre-tdT|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Kazuhiro Oka|
COMMENT     VNTREPLTYPE|Plasmid
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COMMENT                                   "{\\i Sma}I (1721)" "@N (@S)" 5 15.0446 
COMMENT                                   -10.454 0 -10 4.64561 0.932452 #328#) 
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COMMENT                                   "{\\i Sma}I (4338)" "@N (@S)" 5 -14.0204 
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FEATURES             Location/Qualifiers
     misc_feature    12..141
                     /vntifkey="21"
                     /label=5'ITR
     misc_feature    4250..4379
                     /vntifkey="21"
                     /label=3'ITR
     misc_feature    complement(4482..4788)
                     /vntifkey="21"
                     /label=f1\Origin
     misc_feature    complement(5307..6167)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    complement(6312..6985)
                     /vntifkey="21"
                     /label=pUC/ORI
     misc_feature    148..285
                     /vntifkey="21"
                     /label=5IEE
     misc_feature    293..888
                     /vntifkey="21"
                     /label=CMV\promoter
     misc_feature    4102..4229
                     /vntifkey="21"
                     /label=SV40\polyA
     misc_feature    3501..4101
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    922..1974
                     /vntifkey="21"
                     /label=iCre
     misc_feature    1981..2046
                     /vntifkey="21"
                     /label=P2A\Peptide
     misc_feature    2047..3477
                     /vntifkey="21"
                     /label=tdTomato
BASE COUNT     1566 a      1942 c      1884 g      1595 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgtg gagtcgtgac gtgaattacg tcatagggtt 
      181 agggaggtcc tgtattagag gtcacgtgag tgttttgcga cattttgcga caccatgtgg 
      241 tcacgctggg tatttaagcc cgagtgagca cgcagggtct ccattacgcg cctagttatt 
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa 
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa 
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc 
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg 
      841 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcaa gcttggatcc 
      901 ttcgaaaccg gtgctagcac catggtgccc aagaagaaga ggaaagtctc caacctgctg 
      961 actgtgcacc aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag 
     1021 aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc 
     1081 ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct 
     1141 gctgaacctg aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg 
     1201 aagaccatcc aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct 
     1261 cgcccttctg actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg 
     1321 gatgctgggg agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc 
     1381 agatccctga tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc 
     1441 attgcctaca acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc 
     1501 tcccgcaccg atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc 
     1561 acagctggtg tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc 
     1621 tctgtgtctg gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat 
     1681 ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt 
     1741 gaggccaccc accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc 
     1801 tggtctggcc actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc 
     1861 atccctgaaa tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc 
     1921 agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaggatgg ggacgttaac 
     1981 ggaagcggag ccactaactt ctccctgttg aaacaagcag gggatgtcga agagaatccc 
     2041 gggccaatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc 
     2101 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 
     2161 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc 
     2221 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc 
     2281 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 
     2341 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg 
     2401 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag 
     2461 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg 
     2521 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc 
     2581 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc 
     2641 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc 
     2701 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc 
     2761 ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag 
     2821 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc 
     2881 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 
     2941 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac 
     3001 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc 
     3061 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac 
     3121 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta 
     3181 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 
     3241 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg 
     3301 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg 
     3361 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 
     3421 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaaggc 
     3481 gcgcccctgc agggaattct atcgataatc aacctctgga ttacaaaatt tgtgaaagat 
     3541 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     3601 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     3661 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
     3721 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
     3781 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
     3841 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
     3901 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 
     3961 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
     4021 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
     4081 gggccgcctc cccgcatcga taagtaagtg actagaactt gtttattgca gcttataatg 
     4141 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     4201 ctagttgtgg tttgtccaaa ctcatctgca cgcgtggcgc gtcggccgca ggaaccccta 
     4261 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 
     4321 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 
     4381 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 
     4441 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     4501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     4561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     4621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     4681 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     4741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     4801 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     4861 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 
     4921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 
     4981 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     5041 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     5101 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
     5161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     5221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     5281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     5341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     5401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     5461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     5521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     5581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     5641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     5701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     5761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     5821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     5881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     5941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     6001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     6061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     6121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     6181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     6241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     6481 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     6601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     6661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     6721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     6781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     6841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     6901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     6961 ccttttgctg gccttttgct cacatgt 
//
