LOCUS       pCDH-TRE-CMV-Puro        7800 bp    DNA     circular     30-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12861406|
COMMENT     VNTDBDATE|-14021826|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-TRE-CMV-Puro|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    4862..4993
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1798..1916
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    4557..4790
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(6336..7196)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RRE
     misc_feature    1309..1797
                     /vntifkey="21"
                     /label=env
     misc_feature    5518..6191
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    5002..5149
                     /vntifkey="21"
                     /label=SV40\Origin
     misc_feature    1920..2300
                     /vntifkey="21"
                     /label=TRE
     misc_feature    2331..2680
                     /vntifkey="21"
                     /label=CMV\Promoter
     CDS             2699..3295
                     /vntifkey="4"
                     /label=Puro
     misc_feature    3304..3894
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    3895..4485
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    4001..4022
                     /vntifkey="21"
                     /label=PWL-2570\Sequencing\Primer
     misc_feature    5370..5389
                     /vntifkey="21"
                     /label=AdxShuttle-MA-378-R
     misc_feature    complement(2476..2495)
                     /vntifkey="21"
                     /label=pCDH-TRE-2495R
BASE COUNT     1916 a      1960 c      2002 g      1922 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcgg 
     1921 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga 
     1981 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat 
     2041 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag 
     2101 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga 
     2161 acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac 
     2221 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac 
     2281 ttcctaccct cgtaggatcc ccgggctagc gcggccgcga attcgtcgac actagtatta 
     2341 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 
     2401 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 
     2461 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 
     2521 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 
     2581 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc 
     2641 ctggagacgc catccacgct gttttgacct ccatagaaga ttctagagct aggccaccat 
     2701 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 
     2761 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 
     2821 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 
     2881 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 
     2941 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 
     3001 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 
     3061 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 
     3121 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 
     3181 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 
     3241 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga 
     3301 attaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 
     3361 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 
     3421 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 
     3481 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 
     3541 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 
     3601 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 
     3661 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 
     3721 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 
     3781 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 
     3841 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctaatcaa 
     3901 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     3961 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     4021 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     4081 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     4141 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     4201 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     4261 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 
     4321 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     4381 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     4441 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc 
     4501 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 
     4561 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg 
     4621 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
     4681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
     4741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 
     4801 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 
     4861 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     4921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     4981 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg 
     5041 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     5101 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca 
     5161 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     5221 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     5281 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     5341 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     5401 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     5461 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     5521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     5581 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     5641 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     5701 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     5761 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     5821 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     5881 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     5941 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     6001 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     6061 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     6121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     6181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     6241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     6301 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     6361 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     6421 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     6481 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     6541 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     6601 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     6661 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     6721 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     6781 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     6841 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     6901 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     6961 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     7021 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     7081 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     7141 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     7201 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     7261 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     7321 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
     7381 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 
     7441 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 
     7501 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 
     7561 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 
     7621 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 
     7681 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 
     7741 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg 
//
