LOCUS       CK530_1_TCF21           7057 bp ds-DNA     circular     01-JUL-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           322..342
                      /label="mCMV_seq"
                      /note="sequence: GCTGTTTTGACCTCCATAGAA"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           348..367
                      /label="MPS324_mCMV_fwd2"
                      /note="sequence: CGGGACCGATCCAGCCTACC"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     373..392
                      /label="attB1_fragment"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature     387..397
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     398..934
                      /label="cDNA"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     CDS              398..1000
                      /label="Translation 398-1000"
     misc_feature     939..960
                      /label="attB2_fragment"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(942..960)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(946..962)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(946..963)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 946..1026
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     951..960
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              965..991
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     primer           complement(965..991)
                      /label="MPS323_HA_rev"
                      /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     terminator       992..1000
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer           998..1026
                      /label="MPS311_BC_fwd2"
                      /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer           complement(1001..1026)
                      /label="MPS312-gBlock_attR2-DD_rev"
                      /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     primer_bind      1004..1026
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      1004..1026
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer           1004..1026
                      /label="MPS287-barcode-fwd"
                      /note="sequence: ATACTCAGAAGATGTCACTCACC"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 1027..1096
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1031..1053
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1054..1073
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1074..1096)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1097..1100
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1101..1123)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(1101..1123)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer           complement(1101..1123)
                      /label="MPS288-barcode-rev"
                      /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 1101..1132
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1124..1148
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1133..1166
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           complement(1154..1175)
                      /label="MPS294_barcode_rev2"
                      /note="sequence: TCTTATCATGTCTGGATCGCGG"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal     1158..1292
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1167..1322
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 1323..4033
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(1564..1598)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(1610..1625)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(1834..1853)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      1955..1977
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2016..2034)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           2090..2109
                      /label="MPS112_AmpR_prom_fwd"
                      /note="sequence: cggggaaatgtgcgcggaac"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     promoter         2102..2206
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2207..3067
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2425..2444)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer           complement(2425..2444)
                      /label="MPS101_Amp_rev"
                      /note="sequence: ataataccgcgccacatagc"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     rep_origin       3238..3826
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      3727..3746
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      3980..3997
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4034..4122
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4123..6991
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4203..4224
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4239..4269
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4277..4293
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4301..4317
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    4343..4405
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     4581..4662
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      4689..4708
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           complement(4755..4778)
                      /label="MPS318_resistance_rev"
                      /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              complement(4779..5378)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5264..5283
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     primer           5379..5396
                      /label="MPS315_P2A-rev"
                      /note="sequence: GGGGCCGGGGTTCTCCTC"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(5379..5435)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(5445..6188)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(5586..5605)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer           5789..5808
                      /label="qMPS015F"
                      /note="sequence: GAGCGTACAGTGCGTTCTCC"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     primer_bind      5831..5850
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer           complement(5885..5904)
                      /label="qMPS015R"
                      /note="sequence: CGGGGCTAAAGTGCATCTCG"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     CDS              complement(6189..6212)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6212..6221)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6241..6452)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(6453..6832)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      6981..7000
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 6992..7024
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7025..7057
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7034..7052)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCATG TCCACCGGCT CCCTCAGCGA
      421 TGTGGAGGAC CTTCAAGAGG TGGAGATGTT GGAATGTGAC GGGTTGAAAA TGGATTCGAA
      481 CAAGGAATTT GTGACTTCCA ACGAGAGCAC CGAGGAGAGC TCCAACTGCG AGAATGGGTC
      541 TCCCCAGAAG GGCCGCGGCG GCCTGGGCAA GAGGAGGAAG GCGCCCACCA AGAAGAGCCC
      601 CCTGAGCGGG GTCAGCCAGG AGGGGAAGCA GGTCCAGCGC AACGCCGCCA ACGCGCGAGA
      661 GCGGGCCCGC ATGCGAGTGC TGAGCAAGGC CTTCTCCAGA CTCAAGACCA CCCTGCCCTG
      721 GGTGCCCCCC GACACCAAGC TCTCCAAGCT GGACACGCTC AGGCTGGCGT CCAGCTACAT
      781 CGCCCACTTG AGGCAGATCC TGGCTAACGA CAAATACGAG AACGGGTACA TTCACCCGGT
      841 CAACCTGACG TGGCCCTTTA TGGTGGCCGG GAAACCCGAG AGTGACCTGA AAGAAGTGGT
      901 GACCGCGAGC CGCTTATGTG GAACCACCGC GTCCTTGGAC CCAGCTTTCT TGTACAAAGT
      961 GGTATACCCA TACGATGTTC CAGATTACGC TTAGTAATGA GAAATACTCA GAAGATGTCA
     1021 CTCACCATAG TCCGCAGTGA AAGATAAATG ATCNNNNNNN NNNNNNNNNN NNNGTTTTAG
     1081 AGCTAGAAAT AGCAAGTAGG CCACTGAGCA AGAACTATGA ATGAGATCTT TAGACATATT
     1141 GCGGCCGCTT TCTCCGCGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA
     1201 AAACTAGAAT GCAGTGAAAA AAATGCCTTA TTTGTGAAAT TTGTGATGCT ATTGCCTTAT
     1261 TTGTAACCAT TATAAGCTGC AATAAACAAG TTATCTATAA CAAGAAAATA TATATATAAT
     1321 AAGTTATCAC GTAAGTAGAA CATGAAATAA CAATATAATT ATCGTATGAG TTAAATCTTA
     1381 AAAGTCACGT AAAAGATAAT CATGCGTCAT TTTGACTCAC GCGGTCGTTA TAGTTCAAAA
     1441 TCAGTGACAC TTACCGCATT GACAAGCACG CCTCACGGGA GCTCCAAGCG GCGACTGAGA
     1501 TGTCCTAAAT GCACAGCGAC GGATTCGCGC TATTTAGAAA GAGAGAGCAA TATTTCAAGA
     1561 ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT AAGAATTCAC TGGCCGTCGT
     1621 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA
     1681 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA
     1741 GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG
     1801 CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT
     1861 AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC
     1921 GGCATCCGCT TACAGACAAG CTGTGACCAT GTCTCCGGGA GCTGCATGTG TCAGAGGTTT
     1981 TCACCGTCAT CACCGAAACA CGCGAGTTCG AAAGGGCCTC GTGATACGCC TATTTTTATA
     2041 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT
     2101 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
     2161 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA
     2221 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
     2281 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT
     2341 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC
     2401 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG
     2461 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC
     2521 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
     2581 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA
     2641 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC
     2701 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
     2761 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT
     2821 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
     2881 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGTTCACGCG GTATCATTGC
     2941 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA
     3001 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA
     3061 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT
     3121 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA
     3181 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
     3241 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC
     3301 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
     3361 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA
     3421 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC
     3481 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
     3541 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA
     3601 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG
     3661 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT
     3721 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA
     3781 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
     3841 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT
     3901 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
     3961 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGATGAGC GCCCAATACG
     4021 CAAACCGCCT CTCACCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
     4081 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT
     4141 CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA
     4201 ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
     4261 CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT
     4321 GATTACGCCA AGGTCGACTT AACCCTAGAA AGATAATCAT ATTGTGACGT ACGTTAAAGA
     4381 TAATCATGCG TAAAATTGAC GCATGTGTTT TATCGGTCTG TATATCGAGG TTTATTTATT
     4441 AATTTGAATA GATATTAAGT TTTATTATAT TTACACTTAC ATACTAATAA TAAATTCAAC
     4501 AAACAATTTA TTTATGTTTA TTTATTTATT AAAAAAAAAC AAAAACTCAA AATTTCTTCT
     4561 ATAAAGTAAC AAAACTTTTA GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT
     4621 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT
     4681 CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC
     4741 TACAAATGTG GTATGGCTGA TTATGATCCT CTGGAGATTC ATGCGCCAGG TTTTCTGGTC
     4801 ATGCACCACG TTCGTGGGCC CTCAGGAACC TCCACGTCAG CGGTAACTGT GAAGCCCAGT
     4861 CGCTCGTAAA AAGGAAGATT CCGGGGAGCA CTGGTTTCGA GGAACGCCGG CACGCCAGCC
     4921 CGTTCCGCGG CCTCAACACC AGGAAGAACG ACAGCGCTTC CCAAACCTTT ACCTTGGTGA
     4981 TCTGGGCTGA CACCGACAGT GGCAAGAAAC CACGCAGGCT CCTTTGGTCT ATGAGGAGCC
     5041 AGCAAGCCCT CCATTTGCTG TTGTGCCGCC AACCTACTCC CTGACAGCTC GGCCATCCGC
     5101 GGACCTATTT CCGCGAACAC TGCGCCGGCT TCGACGCTTT CCGGAGTAGT CCAGACCGCA
     5161 ACTGCGGCAC CATCGTCCGC AACCCAAACT TTACCAATGT CCAGACCCAC GCGCGTAAGA
     5221 AACAGCTCTT GCAACTCAGT TACCCTCTCT ATGTGTCTGT CCGGATCTAC GGTATGGCGG
     5281 GTCGCTGGAT AATCTGCAAA TGCTGCGGCA AGTGTTCGGA CAGCCCGTGG TACATCGTCA
     5341 CGGGTAGCGA GCCTAACAGT GGGCTTATAT TCCGTCATGG GGCCGGGGTT CTCCTCCACG
     5401 TCGCCGGCCT GCTTCAGCAG GCTGAAGTTG GTGGCGCCGC TGCCCCCGGG GAGCATGTCA
     5461 AGGTCAAAAT CGTCAAGAGC GTCAGCAGGC AGCATATCAA GGTCAAAGTC GTCAAGGGCA
     5521 TCGGCTGGGA GCATGTCTAA GTCAAAATCG TCAAGGGCGT CGGTCGGCCC GCCGCTTTCG
     5581 CACTTTAGCT GTTTCTCCAG GCCACATATG ATTAGTTCCA GGCCGAAAAG GAAGGCAGGT
     5641 TCGGCTCCCT GCCGGTCGAA CAGCTCAATT GCTTGTTTCA GAAGTGGGGG CATAGAATCG
     5701 GTGGTAGGTG TCTCTCTTTC CTCTTTTGCT ACTTGATGCT CCTGTTCCTC CAATACGCAG
     5761 CCCAGTGTAA AGTGGCCCAC GGCGGACAGA GCGTACAGTG CGTTCTCCAG GGAGAAGCCT
     5821 TGCTGACACA GGAACGCGAG CTGATTTTCC AGGGTTTCGT ACTGTTTCTC TGTTGGGCGG
     5881 GTGCCGAGAT GCACTTTAGC CCCGTCACGA TGTGAGAGGA GAGCACAGCG GTATGACTTG
     5941 GCGTTGTTCC GCAGAAAGTC TTGCCATGAC TCGCCTTCCA GGGGGCAGGA GTGGGTATGA
     6001 TGCCTGTCCA GCATCTCGAT TGGCAGGGCA TCGAGCAGGG CCCGCTTGTT CTTCACGTGC
     6061 CAGTACAGGG TAGGCTGCTC AACTCCCAGC TTTTGAGCGA GTTTCCTTGT CGTCAGGCCT
     6121 TCGATACCGA CTCCATTGAG TAATTCCAGA GCAGAGTTTA TGACTTTGCT CTTGTCCAGT
     6181 CTAGACATCT TATCGTCATC GTCTTTGTAA TCCATGGTGG CGGATCCCGC GTCACGACAC
     6241 CTGTGTTCTG GCGGCAAACC CGTTGCGAAA AAGAACGTTC ACGGCGACTA CTGCACTTAT
     6301 ATACGGTTCT CCCCCACCCT CGGGAAAAAG GCGGAGCCAG TACACGACAT CACTTTCCCA
     6361 GTTTACCCCG CGCCACCTTC TCTAGGCACC GGTTCAATTG CCGACCCCTC CCCCCAACTT
     6421 CTCGGGCACT GTGGGCCATG TGCGCTCTGC CCCATGGTAA TAGCCATGAC TAATACGTAG
     6481 ATGTACTGCC AAGTAGGAAA GTGCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC
     6541 CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT
     6601 GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA AGTGCCTATT
     6661 GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT
     6721 CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA
     6781 ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAGTCA ATAATCAATG TCATAAGCTC
     6841 TAATCGCATA ATCGAGCTAA GAGCGATTAA TATGCTTAGC TAGCTTAGCT AAGCTAGATT
     6901 TCTCTATTCT CCCACAATAT ATAGCAGCTA TTAGCTTAGC AGTCCGAATA CGATCGTCCA
     6961 CGATCCATAA GCGTAACACA TTTGAGTAGC GACTGACGGG CACCGGAGCC ACTCGAGTGG
     7021 AATTACTGAC GGGCACCGGA GCCACTCGAG TGGAATT
//