LOCUS       CK530_4_CEBPD           7330 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..397
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     398..1204
                      /label="CEBPD"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     CDS              398..1273
                      /label="Translation 398-1273"
     misc_feature     complement(1215..1233)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1219..1235)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1219..1236)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1219..1299
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1224..1233
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1238..1264
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1265..1273
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      1277..1299
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      1277..1299
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 1300..1369
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1304..1326
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1327..1346
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1347..1369)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1370..1373
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1374..1396)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(1374..1396)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 1374..1405
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1397..1421
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1406..1439
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1431..1565
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1440..1595
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 1596..4306
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(1837..1871)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(1883..1898)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2107..2126)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2228..2250
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2289..2307)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2375..2479
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2480..3340
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2698..2717)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       3511..4099
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4000..4019
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4253..4270
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4307..4395
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4396..7264
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4476..4497
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4512..4542
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4550..4566
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4574..4590
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    4616..4678
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     4854..4935
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      4962..4981
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5052..5651)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5537..5556
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(5652..5708)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(5718..6461)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(5859..5878)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6104..6123
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(6462..6485)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6485..6494)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6514..6725)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(6726..7105)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7254..7273
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7265..7297
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7298..7330
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7307..7325)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCATG AGCGCCGCGC TCTTCAGCCT
      421 GGACGGCCCG GCGCGCGGCG CGCCCTGGCC TGCGGAGCCT GCGCCCTTCT ACGAACCGGG
      481 CCGGGCGGGC AAGCCGGGCC GCGGGGCCGA GCCAGGGGCC CTAGGCGAGC CAGGCGCCGC
      541 CGCCCCCGCC ATGTACGACG ACGAGAGCGC CATCGACTTC AGCGCCTACA TCGACTCCAT
      601 GGCCGCCGTG CCCACCCTGG AGCTGTGCCA CGACGAGCTC TTCGCCGACC TCTTCAACAG
      661 CAATCACAAG GCGGGCGGCG CGGGGCCCCT GGAGCTTCTT CCCGGCGGCC CCGCGCGCCC
      721 CTTGGGCCCG GGCCCTGCCG CTCCCCGCCT GCTCAAGCGC GAGCCCGACT GGGGCGACGG
      781 CGACGCGCCC GGCTCGCTGT TGCCCGCGCA GGTGGCCGCG TGCGCACAGA CCGTGGTGAG
      841 CTTGGCGGCC GCAGGGCAGC CCACCCCGCC CACGTCGCCG GAGCCGCCGC GCAGCAGCCC
      901 CAGGCAGACC CCCGCGCCCG GCCCCGCCCG GGAGAAGAGC GCCGGCAAGA GGGGCCCGGA
      961 CCGCGGCAGC CCCGAGTACC GGCAGCGGCG CGAGCGCAAC AACATCGCCG TGCGCAAGAG
     1021 CCGCGACAAG GCCAAGCGGC GCAACCAGGA GATGCAGCAG AAGTTGGTGG AGCTGTCGGC
     1081 TGAGAACGAG AAGCTGCACC AGCGCGTGGA GCAGCTCACG CGGGACCTGG CCGGCCTCCG
     1141 GCAGTTCTTC AAGCAGCTGC CCAGCCCGCC CTTCCTGCCG GCCGCCGGGA CAGCAGACTG
     1201 CCGGTGAATC CACCCAGCTT TCTTGTACAA AGTGGTATAC CCATACGATG TTCCAGATTA
     1261 CGCTTAGTAA TGAGAAATAC TCAGAAGATG TCACTCACCA TAGTCCGCAG TGAAAGATAA
     1321 ATGATCNNNN NNNNNNNNNN NNNNNNGTTT TAGAGCTAGA AATAGCAAGT AGGCCACTGA
     1381 GCAAGAACTA TGAATGAGAT CTTTAGACAT ATTGCGGCCG CTTTCTCCGC GATCCAGACA
     1441 TGATAAGATA CATTGATGAG TTTGGACAAA CCAAAACTAG AATGCAGTGA AAAAAATGCC
     1501 TTATTTGTGA AATTTGTGAT GCTATTGCCT TATTTGTAAC CATTATAAGC TGCAATAAAC
     1561 AAGTTATCTA TAACAAGAAA ATATATATAT AATAAGTTAT CACGTAAGTA GAACATGAAA
     1621 TAACAATATA ATTATCGTAT GAGTTAAATC TTAAAAGTCA CGTAAAAGAT AATCATGCGT
     1681 CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC
     1741 ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG
     1801 CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA
     1861 TCTTTCTAGG GTTAAGAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
     1921 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
     1981 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG
     2041 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC
     2101 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC
     2161 CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC
     2221 CATGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACACGCGAGT
     2281 TCGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
     2341 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
     2401 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
     2461 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
     2521 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
     2581 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
     2641 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
     2701 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
     2761 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
     2821 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
     2881 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
     2941 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
     3001 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
     3061 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
     3121 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
     3181 AGCCGGTGAG CGTGGTTCAC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
     3241 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
     3301 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
     3361 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
     3421 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
     3481 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
     3541 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
     3601 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
     3661 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
     3721 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
     3781 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
     3841 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
     3901 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
     3961 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
     4021 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
     4081 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
     4141 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT
     4201 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC
     4261 AGTGAGCGAG GAAGCGGATG AGCGCCCAAT ACGCAAACCG CCTCTCACCG CGCGTTGGCC
     4321 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA
     4381 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
     4441 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG
     4501 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
     4561 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGGTCGA CTTAACCCTA
     4621 GAAAGATAAT CATATTGTGA CGTACGTTAA AGATAATCAT GCGTAAAATT GACGCATGTG
     4681 TTTTATCGGT CTGTATATCG AGGTTTATTT ATTAATTTGA ATAGATATTA AGTTTTATTA
     4741 TATTTACACT TACATACTAA TAATAAATTC AACAAACAAT TTATTTATGT TTATTTATTT
     4801 ATTAAAAAAA AACAAAAACT CAAAATTTCT TCTATAAAGT AACAAAACTT TTAGCAGTGA
     4861 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
     4921 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
     4981 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
     5041 CCTCTGGAGA TTCATGCGCC AGGTTTTCTG GTCATGCACC ACGTTCGTGG GCCCTCAGGA
     5101 ACCTCCACGT CAGCGGTAAC TGTGAAGCCC AGTCGCTCGT AAAAAGGAAG ATTCCGGGGA
     5161 GCACTGGTTT CGAGGAACGC CGGCACGCCA GCCCGTTCCG CGGCCTCAAC ACCAGGAAGA
     5221 ACGACAGCGC TTCCCAAACC TTTACCTTGG TGATCTGGGC TGACACCGAC AGTGGCAAGA
     5281 AACCACGCAG GCTCCTTTGG TCTATGAGGA GCCAGCAAGC CCTCCATTTG CTGTTGTGCC
     5341 GCCAACCTAC TCCCTGACAG CTCGGCCATC CGCGGACCTA TTTCCGCGAA CACTGCGCCG
     5401 GCTTCGACGC TTTCCGGAGT AGTCCAGACC GCAACTGCGG CACCATCGTC CGCAACCCAA
     5461 ACTTTACCAA TGTCCAGACC CACGCGCGTA AGAAACAGCT CTTGCAACTC AGTTACCCTC
     5521 TCTATGTGTC TGTCCGGATC TACGGTATGG CGGGTCGCTG GATAATCTGC AAATGCTGCG
     5581 GCAAGTGTTC GGACAGCCCG TGGTACATCG TCACGGGTAG CGAGCCTAAC AGTGGGCTTA
     5641 TATTCCGTCA TGGGGCCGGG GTTCTCCTCC ACGTCGCCGG CCTGCTTCAG CAGGCTGAAG
     5701 TTGGTGGCGC CGCTGCCCCC GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA
     5761 GGCAGCATAT CAAGGTCAAA GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA
     5821 TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT
     5881 ATGATTAGTT CCAGGCCGAA AAGGAAGGCA GGTTCGGCTC CCTGCCGGTC GAACAGCTCA
     5941 ATTGCTTGTT TCAGAAGTGG GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT
     6001 GCTACTTGAT GCTCCTGTTC CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC
     6061 AGAGCGTACA GTGCGTTCTC CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT
     6121 TCCAGGGTTT CGTACTGTTT CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCA
     6181 CGATGTGAGA GGAGAGCACA GCGGTATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT
     6241 GACTCGCCTT CCAGGGGGCA GGAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGGCAGG
     6301 GCATCGAGCA GGGCCCGCTT GTTCTTCACG TGCCAGTACA GGGTAGGCTG CTCAACTCCC
     6361 AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG CCTTCGATAC CGACTCCATT GAGTAATTCC
     6421 AGAGCAGAGT TTATGACTTT GCTCTTGTCC AGTCTAGACA TCTTATCGTC ATCGTCTTTG
     6481 TAATCCATGG TGGCGGATCC CGCGTCACGA CACCTGTGTT CTGGCGGCAA ACCCGTTGCG
     6541 AAAAAGAACG TTCACGGCGA CTACTGCACT TATATACGGT TCTCCCCCAC CCTCGGGAAA
     6601 AAGGCGGAGC CAGTACACGA CATCACTTTC CCAGTTTACC CCGCGCCACC TTCTCTAGGC
     6661 ACCGGTTCAA TTGCCGACCC CTCCCCCCAA CTTCTCGGGC ACTGTGGGCC ATGTGCGCTC
     6721 TGCCCCATGG TAATAGCCAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTGCCAT
     6781 AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG
     6841 GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC
     6901 TCCACCCATT GACGTCAATG GAAAGTGCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT
     6961 ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT
     7021 TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT
     7081 TAATAACTAG TCAATAATCA ATGTCATAAG CTCTAATCGC ATAATCGAGC TAAGAGCGAT
     7141 TAATATGCTT AGCTAGCTTA GCTAAGCTAG ATTTCTCTAT TCTCCCACAA TATATAGCAG
     7201 CTATTAGCTT AGCAGTCCGA ATACGATCGT CCACGATCCA TAAGCGTAAC ACATTTGAGT
     7261 AGCGACTGAC GGGCACCGGA GCCACTCGAG TGGAATTACT GACGGGCACC GGAGCCACTC
     7321 GAGTGGAATT
//