LOCUS       CK530_5_EMX2            7267 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..391
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     392..1154
                      /label="EMX2"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     CDS              392..1210
                      /label="Translation 392-1210"
     misc_feature     complement(1155..1170)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1156..1172)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1156..1173)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1156..1236
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1161..1170
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1175..1201
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1202..1210
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      1214..1236
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      1214..1236
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 1237..1306
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1241..1263
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1264..1283
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1284..1306)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1307..1310
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1311..1333)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(1311..1333)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 1311..1342
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1334..1358
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1343..1376
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1368..1502
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1377..1532
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 1533..4243
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(1774..1808)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(1820..1835)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2044..2063)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2165..2187
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2226..2244)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2312..2416
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2417..3277
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2635..2654)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       3448..4036
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      3937..3956
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4190..4207
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4244..4332
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4333..7201
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4413..4434
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4449..4479
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4487..4503
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4511..4527
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    4553..4615
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     4791..4872
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      4899..4918
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(4989..5588)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5474..5493
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(5589..5645)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(5655..6398)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(5796..5815)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6041..6060
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(6399..6422)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6422..6431)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6451..6662)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(6663..7042)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7191..7210
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7202..7234
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7235..7267
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7244..7262)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGttgg CATGTTCCAG CCAGCACCCA AGCGCTGCTT
      421 CACTATAGAG TCGCTGGTGG CAAAAGACAG TCCCCTGCCA GCATCGCGCT CCGAGGACCC
      481 CATTCGTCCA GCAGCACTCA GCTACGCTAA CTCCAGTCCC ATAAATCCGT TCCTCAACGG
      541 CTTCCACTCA GCAGCCGCTG CAGCCGCCGG TAGGGGCGTA TACTCAAACC CGGATTTAGT
      601 GTTCGCCGAA GCGGTTTCGC ATCCTCCAAA CCCCGCAGTA CCAGTACACC CAGTGCCTCC
      661 GCCTCATGCT CTTGCCGCTC ATCCCCTACC CTCATCACAC TCGCCACATC CCCTATTCGC
      721 CTCACAGCAG CGGGATCCGT CTACCTTCTA CCCCTGGCTC ATCCACCGCT ACCGATATCT
      781 GGGTCATCGT TTCCAAGGGA ACGACACTAG CCCCGAGAGT TTCCTTTTGC ACAACGCGCT
      841 GGCCCGAAAG CCCAAGCGGA TCCGAACCGC CTTCTCCCCG TCCCAGCTTC TAAGGCTGGA
      901 ACACGCCTTT GAGAAGAATC ACTACGTGGT GGGCGCCGAA AGGAAGCAGC TGGCACACAG
      961 CCTCAGCCTC ACGGAAACTC AGGTAAAAGT ATGGTTTCAG AACCGAAGAA CAAAGTTCAA
     1021 AAGGCAGAAG CTGGAGGAAG AAGGCTCAGA TTCGCAACAA AAGAAAAAAG GGACGCACCA
     1081 TATTAACCGG TGGAGAATCG CCACCAAGCA GGCGAGTCCG GAGGAAATAG ACGTGACCTC
     1141 AGATGATTAC CCAACTTTCT TGTACAAAGT GGTATACCCA TACGATGTTC CAGATTACGC
     1201 TTAGTAATGA GAAATACTCA GAAGATGTCA CTCACCATAG TCCGCAGTGA AAGATAAATG
     1261 ATCNNNNNNN NNNNNNNNNN NNNGTTTTAG AGCTAGAAAT AGCAAGTAGG CCACTGAGCA
     1321 AGAACTATGA ATGAGATCTT TAGACATATT GCGGCCGCTT TCTCCGCGAT CCAGACATGA
     1381 TAAGATACAT TGATGAGTTT GGACAAACCA AAACTAGAAT GCAGTGAAAA AAATGCCTTA
     1441 TTTGTGAAAT TTGTGATGCT ATTGCCTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG
     1501 TTATCTATAA CAAGAAAATA TATATATAAT AAGTTATCAC GTAAGTAGAA CATGAAATAA
     1561 CAATATAATT ATCGTATGAG TTAAATCTTA AAAGTCACGT AAAAGATAAT CATGCGTCAT
     1621 TTTGACTCAC GCGGTCGTTA TAGTTCAAAA TCAGTGACAC TTACCGCATT GACAAGCACG
     1681 CCTCACGGGA GCTCCAAGCG GCGACTGAGA TGTCCTAAAT GCACAGCGAC GGATTCGCGC
     1741 TATTTAGAAA GAGAGAGCAA TATTTCAAGA ATGCATGCGT CAATTTTACG CAGACTATCT
     1801 TTCTAGGGTT AAGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
     1861 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
     1921 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT
     1981 GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT
     2041 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC
     2101 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCAT
     2161 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACA CGCGAGTTCG
     2221 AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA
     2281 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
     2341 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA
     2401 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC
     2461 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
     2521 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT
     2581 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
     2641 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA
     2701 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT
     2761 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
     2821 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA
     2881 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
     2941 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA
     3001 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC
     3061 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC
     3121 CGGTGAGCGT GGTTCACGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG
     3181 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
     3241 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA
     3301 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT
     3361 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA
     3421 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     3481 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     3541 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     3601 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     3661 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     3721 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     3781 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     3841 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     3901 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     3961 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     4021 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     4081 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
     4141 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
     4201 GAGCGAGGAA GCGGATGAGC GCCCAATACG CAAACCGCCT CTCACCGCGC GTTGGCCGAT
     4261 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC
     4321 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
     4381 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
     4441 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
     4501 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGGTCGACTT AACCCTAGAA
     4561 AGATAATCAT ATTGTGACGT ACGTTAAAGA TAATCATGCG TAAAATTGAC GCATGTGTTT
     4621 TATCGGTCTG TATATCGAGG TTTATTTATT AATTTGAATA GATATTAAGT TTTATTATAT
     4681 TTACACTTAC ATACTAATAA TAAATTCAAC AAACAATTTA TTTATGTTTA TTTATTTATT
     4741 AAAAAAAAAC AAAAACTCAA AATTTCTTCT ATAAAGTAAC AAAACTTTTA GCAGTGAAAA
     4801 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC
     4861 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG
     4921 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCCT
     4981 CTGGAGATTC ATGCGCCAGG TTTTCTGGTC ATGCACCACG TTCGTGGGCC CTCAGGAACC
     5041 TCCACGTCAG CGGTAACTGT GAAGCCCAGT CGCTCGTAAA AAGGAAGATT CCGGGGAGCA
     5101 CTGGTTTCGA GGAACGCCGG CACGCCAGCC CGTTCCGCGG CCTCAACACC AGGAAGAACG
     5161 ACAGCGCTTC CCAAACCTTT ACCTTGGTGA TCTGGGCTGA CACCGACAGT GGCAAGAAAC
     5221 CACGCAGGCT CCTTTGGTCT ATGAGGAGCC AGCAAGCCCT CCATTTGCTG TTGTGCCGCC
     5281 AACCTACTCC CTGACAGCTC GGCCATCCGC GGACCTATTT CCGCGAACAC TGCGCCGGCT
     5341 TCGACGCTTT CCGGAGTAGT CCAGACCGCA ACTGCGGCAC CATCGTCCGC AACCCAAACT
     5401 TTACCAATGT CCAGACCCAC GCGCGTAAGA AACAGCTCTT GCAACTCAGT TACCCTCTCT
     5461 ATGTGTCTGT CCGGATCTAC GGTATGGCGG GTCGCTGGAT AATCTGCAAA TGCTGCGGCA
     5521 AGTGTTCGGA CAGCCCGTGG TACATCGTCA CGGGTAGCGA GCCTAACAGT GGGCTTATAT
     5581 TCCGTCATGG GGCCGGGGTT CTCCTCCACG TCGCCGGCCT GCTTCAGCAG GCTGAAGTTG
     5641 GTGGCGCCGC TGCCCCCGGG GAGCATGTCA AGGTCAAAAT CGTCAAGAGC GTCAGCAGGC
     5701 AGCATATCAA GGTCAAAGTC GTCAAGGGCA TCGGCTGGGA GCATGTCTAA GTCAAAATCG
     5761 TCAAGGGCGT CGGTCGGCCC GCCGCTTTCG CACTTTAGCT GTTTCTCCAG GCCACATATG
     5821 ATTAGTTCCA GGCCGAAAAG GAAGGCAGGT TCGGCTCCCT GCCGGTCGAA CAGCTCAATT
     5881 GCTTGTTTCA GAAGTGGGGG CATAGAATCG GTGGTAGGTG TCTCTCTTTC CTCTTTTGCT
     5941 ACTTGATGCT CCTGTTCCTC CAATACGCAG CCCAGTGTAA AGTGGCCCAC GGCGGACAGA
     6001 GCGTACAGTG CGTTCTCCAG GGAGAAGCCT TGCTGACACA GGAACGCGAG CTGATTTTCC
     6061 AGGGTTTCGT ACTGTTTCTC TGTTGGGCGG GTGCCGAGAT GCACTTTAGC CCCGTCACGA
     6121 TGTGAGAGGA GAGCACAGCG GTATGACTTG GCGTTGTTCC GCAGAAAGTC TTGCCATGAC
     6181 TCGCCTTCCA GGGGGCAGGA GTGGGTATGA TGCCTGTCCA GCATCTCGAT TGGCAGGGCA
     6241 TCGAGCAGGG CCCGCTTGTT CTTCACGTGC CAGTACAGGG TAGGCTGCTC AACTCCCAGC
     6301 TTTTGAGCGA GTTTCCTTGT CGTCAGGCCT TCGATACCGA CTCCATTGAG TAATTCCAGA
     6361 GCAGAGTTTA TGACTTTGCT CTTGTCCAGT CTAGACATCT TATCGTCATC GTCTTTGTAA
     6421 TCCATGGTGG CGGATCCCGC GTCACGACAC CTGTGTTCTG GCGGCAAACC CGTTGCGAAA
     6481 AAGAACGTTC ACGGCGACTA CTGCACTTAT ATACGGTTCT CCCCCACCCT CGGGAAAAAG
     6541 GCGGAGCCAG TACACGACAT CACTTTCCCA GTTTACCCCG CGCCACCTTC TCTAGGCACC
     6601 GGTTCAATTG CCGACCCCTC CCCCCAACTT CTCGGGCACT GTGGGCCATG TGCGCTCTGC
     6661 CCCATGGTAA TAGCCATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA GTGCCATAAG
     6721 GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC
     6781 GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC
     6841 ACCCATTGAC GTCAATGGAA AGTGCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT
     6901 GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC CGTAAGTTAT
     6961 GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA TTACTATTAA
     7021 TAACTAGTCA ATAATCAATG TCATAAGCTC TAATCGCATA ATCGAGCTAA GAGCGATTAA
     7081 TATGCTTAGC TAGCTTAGCT AAGCTAGATT TCTCTATTCT CCCACAATAT ATAGCAGCTA
     7141 TTAGCTTAGC AGTCCGAATA CGATCGTCCA CGATCCATAA GCGTAACACA TTTGAGTAGC
     7201 GACTGACGGG CACCGGAGCC ACTCGAGTGG AATTACTGAC GGGCACCGGA GCCACTCGAG
     7261 TGGAATT
//