LOCUS       CK530_10_ZBTB16         8539 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..397
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     398..2416
                      /label="ZBTB16"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              398..2482
                      /label="Translation 398-2482"
     misc_feature     complement(2424..2442)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(2428..2444)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(2428..2445)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 2428..2508
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     2433..2442
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2447..2473
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       2474..2482
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      2486..2508
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      2486..2508
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 2509..2578
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  2513..2535
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     2536..2555
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(2556..2578)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 2579..2582
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(2583..2605)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(2583..2605)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 2583..2614
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            2606..2630
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 2615..2648
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     2640..2774
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 2649..2804
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2805..5515
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(3046..3080)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(3092..3107)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(3316..3335)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      3437..3459
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(3498..3516)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         3584..3688
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              3689..4549
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3907..3926)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       4720..5308
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      5209..5228
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      5462..5479
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 5516..5604
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 5605..8473
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     5685..5706
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         5721..5751
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     5759..5775
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      5783..5799
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5825..5887
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     6063..6144
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      6171..6190
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(6261..6860)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      6746..6765
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6861..6917)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6927..7670)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(7068..7087)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      7313..7332
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(7671..7694)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(7694..7703)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(7723..7934)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7935..8314)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      8463..8482
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 8474..8506
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 8507..8539
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(8516..8534)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCATG GATCTGACAA AAATGGGCAT
      421 GATCCAGCTG CAGAACCCTA GCCACCCCAC GGGGCTACTG TGCAAGGCCA ACCAGATGCG
      481 GCTGGCCGGG ACTTTGTGCG ATGTGGTCAT CATGGTGGAC AGCCAGGAGT TCCACGCCCA
      541 CCGGACGGTG CTGGCCTGCA CCAGCAAGAT GTTTGAGATC CTCTTCCACC GCAATAGTCA
      601 ACACTATACT TTGGACTTCC TCTCGCCAAA GACCTTCCAG CAGATTCTGG AGTATGCATA
      661 TACAGCCACG CTGCAAGCCA AGGCGGAGGA CCTGGATGAC CTGCTGTATG CGGCCGAGAT
      721 CCTGGAGATC GAGTACCTGG AGGAACAGTG CCTGAAGATG CTGGAGACCA TCCAGGCCTC
      781 AGACGACAAT GACACGGAGG CCACCATGGC CGATGGCGGG GCCGAGGAAG AAGAGGACCG
      841 CAAGGCTCGG TACCTCAAGA ACATCTTCAT CTCGAAGCAT TCCAGCGAGG AGAGTGGGTA
      901 TGCCAGTGTG GCTGGACAGA GCCTCCCTGG GCCCATGGTG GACCAGAGCC CTTCAGTCTC
      961 CACTTCATTT GGTCTTTCAG CCATGAGTCC CACCAAGGCT GCAGTGGACA GTTTGATGAC
     1021 CATAGGACAG TCTCTCCTGC AGGGAACTCT TCAGCCACCT GCAGGGCCCG AGGAGCCAAC
     1081 TCTGGCTGGG GGTGGGCGGC ACCCTGGGGT GGCTGAGGTG AAGACGGAGA TGATGCAGGT
     1141 GGATGAGGTG CCCAGCCAGG ACAGCCCTGG GGCAGCCGAG TCCAGCATCT CAGGAGGGAT
     1201 GGGGGACAAG GTTGAGGAAA GAGGCAAAGA GGGGCCTGGG ACCCCGACTC GAAGCAGCGT
     1261 CATCACCAGT GCTAGGGAGC TACACTATGG GCGAGAGGAG AGTGCCGAGC AGGTGCCACC
     1321 CCCAGCTGAG GCTGGCCAGG CCCCCACTGG CCGACCTGAG CACCCAGCAC CCCCGCCTGA
     1381 GAAGCATCTG GGCATCTACT CCGTGTTGCC CAACCACAAG GCTGACGCTG TATTGAGCAT
     1441 GCCGTCTTCC GTGACCTCTG GCCTCCACGT GCAGCCTGCC CTGGCTGTCT CCATGGACTT
     1501 CAGCACCTAT GGGGGGCTGC TGCCCCAGGG CTTCATCCAG AGGGAGCTGT TCAGCAAGCT
     1561 GGGGGAGCTG GCTGTGGGCA TGAAGTCAGA GAGCCGGACC ATCGGAGAGC AGTGCAGCGT
     1621 GTGTGGGGTC GAGCTTCCTG ATAACGAGGC TGTGGAGCAG CACAGGAAGC TGCACAGTGG
     1681 GATGAAGACG TACGGGTGCG AGCTCTGCGG GAAGCGGTTC CTGGATAGTT TGCGGCTGAG
     1741 AATGCACTTA CTGGCTCATT CAGCGGGTGC CAAAGCCTTT GTCTGTGATC AGTGCGGTGC
     1801 ACAGTTTTCG AAGGAGGATG CCCTGGAGAC ACACAGGCAG ACCCATACTG GCACTGACAT
     1861 GGCCGTCTTC TGTCTGCTGT GTGGGAAGCG CTTCCAGGCG CAGAGCGCAC TGCAGCAGCA
     1921 CATGGAGGTC CACGCGGGCG TGCGCAGCTA CATCTGCAGT GAGTGCAACC GCACCTTCCC
     1981 CAGCCACACG GCTCTCAAAC GCCACCTGCG CTCACATACA GGCGACCACC CCTACGAGTG
     2041 TGAGTTCTGT GGCAGCTGCT TCCGGGATGA GAGCACACTC AAGAGCCACA AACGCATCCA
     2101 CACGGGTGAG AAACCCTACG AGTGCAATGG CTGTGGCAAG AAGTTCAGCC TCAAGCATCA
     2161 GCTGGAGACG CACTATAGGG TGCACACAGG TGAGAAGCCC TTTGAGTGTA AGCTCTGCCA
     2221 CCAGCGCTCC CGGGACTACT CGGCCATGAT CAAGCACCTG AGAACGCACA ACGGCGCCTC
     2281 GCCCTACCAG TGCACCATCT GCACAGAGTA CTGCCCCAGC CTCTCCTCCA TGCAGAAGCA
     2341 CATGAAGGGC CACAAGCCCG AGGAGATCCC GCCCGACTGG AGGATAGAGA AGACGTACCT
     2401 CTACCTGTGC TATGTGTTGG ACCCAGCTTT CTTGTACAAA GTGGTATACC CATACGATGT
     2461 TCCAGATTAC GCTTAGTAAT GAGAAATACT CAGAAGATGT CACTCACCAT AGTCCGCAGT
     2521 GAAAGATAAA TGATCNNNNN NNNNNNNNNN NNNNNGTTTT AGAGCTAGAA ATAGCAAGTA
     2581 GGCCACTGAG CAAGAACTAT GAATGAGATC TTTAGACATA TTGCGGCCGC TTTCTCCGCG
     2641 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CAAAACTAGA ATGCAGTGAA
     2701 AAAAATGCCT TATTTGTGAA ATTTGTGATG CTATTGCCTT ATTTGTAACC ATTATAAGCT
     2761 GCAATAAACA AGTTATCTAT AACAAGAAAA TATATATATA ATAAGTTATC ACGTAAGTAG
     2821 AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC GTAAAAGATA
     2881 ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC ACTTACCGCA
     2941 TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA ATGCACAGCG
     3001 ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC GTCAATTTTA
     3061 CGCAGACTAT CTTTCTAGGG TTAAGAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG
     3121 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG
     3181 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
     3241 CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT
     3301 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     3361 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     3421 AGCTGTGACC ATGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA
     3481 CACGCGAGTT CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT
     3541 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
     3601 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
     3661 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
     3721 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
     3781 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
     3841 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
     3901 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
     3961 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
     4021 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
     4081 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
     4141 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
     4201 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
     4261 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
     4321 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
     4381 TAAATCTGGA GCCGGTGAGC GTGGTTCACG CGGTATCATT GCAGCACTGG GGCCAGATGG
     4441 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
     4501 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
     4561 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
     4621 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
     4681 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
     4741 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
     4801 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
     4861 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
     4921 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
     4981 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
     5041 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
     5101 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
     5161 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
     5221 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
     5281 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
     5341 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
     5401 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
     5461 CAGCGAGTCA GTGAGCGAGG AAGCGGATGA GCGCCCAATA CGCAAACCGC CTCTCACCGC
     5521 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
     5581 TGAGCGCAAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC
     5641 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC
     5701 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT
     5761 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGGTCGAC
     5821 TTAACCCTAG AAAGATAATC ATATTGTGAC GTACGTTAAA GATAATCATG CGTAAAATTG
     5881 ACGCATGTGT TTTATCGGTC TGTATATCGA GGTTTATTTA TTAATTTGAA TAGATATTAA
     5941 GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT TATTTATGTT
     6001 TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA ACAAAACTTT
     6061 TAGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
     6121 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
     6181 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
     6241 GATTATGATC CTCTGGAGAT TCATGCGCCA GGTTTTCTGG TCATGCACCA CGTTCGTGGG
     6301 CCCTCAGGAA CCTCCACGTC AGCGGTAACT GTGAAGCCCA GTCGCTCGTA AAAAGGAAGA
     6361 TTCCGGGGAG CACTGGTTTC GAGGAACGCC GGCACGCCAG CCCGTTCCGC GGCCTCAACA
     6421 CCAGGAAGAA CGACAGCGCT TCCCAAACCT TTACCTTGGT GATCTGGGCT GACACCGACA
     6481 GTGGCAAGAA ACCACGCAGG CTCCTTTGGT CTATGAGGAG CCAGCAAGCC CTCCATTTGC
     6541 TGTTGTGCCG CCAACCTACT CCCTGACAGC TCGGCCATCC GCGGACCTAT TTCCGCGAAC
     6601 ACTGCGCCGG CTTCGACGCT TTCCGGAGTA GTCCAGACCG CAACTGCGGC ACCATCGTCC
     6661 GCAACCCAAA CTTTACCAAT GTCCAGACCC ACGCGCGTAA GAAACAGCTC TTGCAACTCA
     6721 GTTACCCTCT CTATGTGTCT GTCCGGATCT ACGGTATGGC GGGTCGCTGG ATAATCTGCA
     6781 AATGCTGCGG CAAGTGTTCG GACAGCCCGT GGTACATCGT CACGGGTAGC GAGCCTAACA
     6841 GTGGGCTTAT ATTCCGTCAT GGGGCCGGGG TTCTCCTCCA CGTCGCCGGC CTGCTTCAGC
     6901 AGGCTGAAGT TGGTGGCGCC GCTGCCCCCG GGGAGCATGT CAAGGTCAAA ATCGTCAAGA
     6961 GCGTCAGCAG GCAGCATATC AAGGTCAAAG TCGTCAAGGG CATCGGCTGG GAGCATGTCT
     7021 AAGTCAAAAT CGTCAAGGGC GTCGGTCGGC CCGCCGCTTT CGCACTTTAG CTGTTTCTCC
     7081 AGGCCACATA TGATTAGTTC CAGGCCGAAA AGGAAGGCAG GTTCGGCTCC CTGCCGGTCG
     7141 AACAGCTCAA TTGCTTGTTT CAGAAGTGGG GGCATAGAAT CGGTGGTAGG TGTCTCTCTT
     7201 TCCTCTTTTG CTACTTGATG CTCCTGTTCC TCCAATACGC AGCCCAGTGT AAAGTGGCCC
     7261 ACGGCGGACA GAGCGTACAG TGCGTTCTCC AGGGAGAAGC CTTGCTGACA CAGGAACGCG
     7321 AGCTGATTTT CCAGGGTTTC GTACTGTTTC TCTGTTGGGC GGGTGCCGAG ATGCACTTTA
     7381 GCCCCGTCAC GATGTGAGAG GAGAGCACAG CGGTATGACT TGGCGTTGTT CCGCAGAAAG
     7441 TCTTGCCATG ACTCGCCTTC CAGGGGGCAG GAGTGGGTAT GATGCCTGTC CAGCATCTCG
     7501 ATTGGCAGGG CATCGAGCAG GGCCCGCTTG TTCTTCACGT GCCAGTACAG GGTAGGCTGC
     7561 TCAACTCCCA GCTTTTGAGC GAGTTTCCTT GTCGTCAGGC CTTCGATACC GACTCCATTG
     7621 AGTAATTCCA GAGCAGAGTT TATGACTTTG CTCTTGTCCA GTCTAGACAT CTTATCGTCA
     7681 TCGTCTTTGT AATCCATGGT GGCGGATCCC GCGTCACGAC ACCTGTGTTC TGGCGGCAAA
     7741 CCCGTTGCGA AAAAGAACGT TCACGGCGAC TACTGCACTT ATATACGGTT CTCCCCCACC
     7801 CTCGGGAAAA AGGCGGAGCC AGTACACGAC ATCACTTTCC CAGTTTACCC CGCGCCACCT
     7861 TCTCTAGGCA CCGGTTCAAT TGCCGACCCC TCCCCCCAAC TTCTCGGGCA CTGTGGGCCA
     7921 TGTGCGCTCT GCCCCATGGT AATAGCCATG ACTAATACGT AGATGTACTG CCAAGTAGGA
     7981 AAGTGCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG
     8041 TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC
     8101 CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTGCCTA TTGGCGTTAC TATGGGAACA
     8161 TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT
     8221 ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA CCCCGTAATT
     8281 GATTACTATT AATAACTAGT CAATAATCAA TGTCATAAGC TCTAATCGCA TAATCGAGCT
     8341 AAGAGCGATT AATATGCTTA GCTAGCTTAG CTAAGCTAGA TTTCTCTATT CTCCCACAAT
     8401 ATATAGCAGC TATTAGCTTA GCAGTCCGAA TACGATCGTC CACGATCCAT AAGCGTAACA
     8461 CATTTGAGTA GCGACTGACG GGCACCGGAG CCACTCGAGT GGAATTACTG ACGGGCACCG
     8521 GAGCCACTCG AGTGGAATT
//