LOCUS       CK530_12_LHX1           7738 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..397
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     398..1617
                      /label="LHX1"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     CDS              398..1681
                      /label="Translation 398-1681"
     misc_feature     complement(1623..1641)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1627..1643)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1627..1644)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1627..1707
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1632..1641
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1646..1672
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1673..1681
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      1685..1707
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      1685..1707
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 1708..1777
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1712..1734
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1735..1754
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1755..1777)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1778..1781
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1782..1804)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(1782..1804)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 1782..1813
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1805..1829
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1814..1847
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1839..1973
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1848..2003
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2004..4714
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2245..2279)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2291..2306)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2515..2534)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2636..2658
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2697..2715)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2783..2887
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2888..3748
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3106..3125)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       3919..4507
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4408..4427
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4661..4678
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4715..4803
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4804..7672
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4884..4905
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4920..4950
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4958..4974
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4982..4998
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5024..5086
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5262..5343
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5370..5389
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5460..6059)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5945..5964
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6060..6116)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6126..6869)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6267..6286)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6512..6531
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(6870..6893)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6893..6902)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6922..7133)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7134..7513)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7662..7681
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7673..7705
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7706..7738
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7715..7733)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCATG GTTCACTGTG CCGGCTGCAA
      421 AAGGCCCATC CTGGACCGCT TTCTCTTGAA CGTGCTGGAC AGGGCCTGGC ACGTCAAGTG
      481 CGTCCAGTGC TGTGAATGTA AATGCAACCT GACCGAGAAG TGCTTCTCCA GGGAAGGCAA
      541 ACTCTACTGC AAGAACGACT TCTTCCGGTG TTTCGGTACC AAATGCGCAG GCTGCGCTCA
      601 GGGCATCTCC CCTAGCGACC TGGTGCGGAG AGCGCGGAGC AAAGTGTTTC ACCTGAACTG
      661 CTTCACCTGC ATGATGTGTA ACAAGCAGCT CTCCACTGGC GAGGAACTCT ACATCATCGA
      721 CGAGAATAAG TTCGTCTGCA AAGAGGATTA CCTAAGTAAC AGCAGTGTTG CCAAAGAGAA
      781 CAGCCTTCAC TCGGCCACCA CGGGCAGTGA CCCCAGTTTG TCTCCGGATT CCCAAGACCC
      841 GTCGCAGGAC GACGCCAAGG ACTCGGAGAG CGCCAACGTG TCGGACAAGG AAGCGGGTAG
      901 CAACGAGAAT GACGACCAGA ACCTGGGCGC CAAGCGGCGG GGACCGCGCA CCACCATCAA
      961 AGCCAAGCAG CTGGAGACGC TGAAGGCCGC CTTCGCTGCT ACACCCAAGC CCACCCGCCA
     1021 CATCCGCGAG CAGCTGGCGC AGGAGACCGG CCTCAACATG CGCGTCATTC AGGTCTGGTT
     1081 CCAGAACCGG CGCTCCAAGG AGCGGAGGAT GAAGCAGCTG AGCGCCCTGG GCGCCCGGCG
     1141 CCACGCCTTC TTCCGCAGTC CGCGCCGGAT GCGGCCGCTG GTGGACCGCC TGGAGCCGGG
     1201 CGAGCTCATC CCCAATGGTC CCTTCTCCTT CTACGGAGAT TACCAGAGCG AGTACTACGG
     1261 GCCCGGGGGC AACTACGACT TCTTCCCGCA AGGCCCCCCG TCCTCGCAGG CCCAGACACC
     1321 AGTGGACCTA CCCTTCGTGC CGTCATCTGG GCCGTCCGGG ACGCCCCTGG GTGGCCTGGA
     1381 GCACCCGCTG CCGGGCCACC ACCCGTCGAG CGAGGCGCAG CGGTTTACCG ACATCCTGGC
     1441 GCACCCACCC GGGGACTCGC CCAGCCCCGA GCCCAGCCTG CCCGGGCCTC TGCACTCCAT
     1501 GTCGGCCGAG GTCTTCGGAC CCAGCCCGCC CTTCTCGTCG CTGTCGGTCA ACGGTGGGGC
     1561 GAGCTACGGA AACCACCTGT CCCACCCCCC CGAAATGAAC GAGGCGGCCG TGTGGTTGGA
     1621 CCCAGCTTTC TTGTACAAAG TGGTATACCC ATACGATGTT CCAGATTACG CTTAGTAATG
     1681 AGAAATACTC AGAAGATGTC ACTCACCATA GTCCGCAGTG AAAGATAAAT GATCNNNNNN
     1741 NNNNNNNNNN NNNNGTTTTA GAGCTAGAAA TAGCAAGTAG GCCACTGAGC AAGAACTATG
     1801 AATGAGATCT TTAGACATAT TGCGGCCGCT TTCTCCGCGA TCCAGACATG ATAAGATACA
     1861 TTGATGAGTT TGGACAAACC AAAACTAGAA TGCAGTGAAA AAAATGCCTT ATTTGTGAAA
     1921 TTTGTGATGC TATTGCCTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTATCTATA
     1981 ACAAGAAAAT ATATATATAA TAAGTTATCA CGTAAGTAGA ACATGAAATA ACAATATAAT
     2041 TATCGTATGA GTTAAATCTT AAAAGTCACG TAAAAGATAA TCATGCGTCA TTTTGACTCA
     2101 CGCGGTCGTT ATAGTTCAAA ATCAGTGACA CTTACCGCAT TGACAAGCAC GCCTCACGGG
     2161 AGCTCCAAGC GGCGACTGAG ATGTCCTAAA TGCACAGCGA CGGATTCGCG CTATTTAGAA
     2221 AGAGAGAGCA ATATTTCAAG AATGCATGCG TCAATTTTAC GCAGACTATC TTTCTAGGGT
     2281 TAAGAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA
     2341 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG
     2401 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA
     2461 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT
     2521 CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC
     2581 CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCA TGTCTCCGGG
     2641 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC ACGCGAGTTC GAAAGGGCCT
     2701 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG
     2761 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC
     2821 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
     2881 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG
     2941 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT
     3001 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
     3061 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT
     3121 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA
     3181 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG
     3241 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC
     3301 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC
     3361 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC
     3421 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC
     3481 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT
     3541 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG
     3601 TGGTTCACGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT
     3661 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT
     3721 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA
     3781 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA
     3841 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA
     3901 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC
     3961 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
     4021 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC
     4081 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT
     4141 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG
     4201 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
     4261 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
     4321 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC
     4381 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG
     4441 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT
     4501 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
     4561 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA
     4621 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     4681 AGCGGATGAG CGCCCAATAC GCAAACCGCC TCTCACCGCG CGTTGGCCGA TTCATTAATG
     4741 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAAACCGCCT
     4801 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
     4861 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT
     4921 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC
     4981 ACAGGAAACA GCTATGACCA TGATTACGCC AAGGTCGACT TAACCCTAGA AAGATAATCA
     5041 TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT
     5101 GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA
     5161 CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA
     5221 CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT AGCAGTGAAA AAAATGCTTT
     5281 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
     5341 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT
     5401 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCC TCTGGAGATT
     5461 CATGCGCCAG GTTTTCTGGT CATGCACCAC GTTCGTGGGC CCTCAGGAAC CTCCACGTCA
     5521 GCGGTAACTG TGAAGCCCAG TCGCTCGTAA AAAGGAAGAT TCCGGGGAGC ACTGGTTTCG
     5581 AGGAACGCCG GCACGCCAGC CCGTTCCGCG GCCTCAACAC CAGGAAGAAC GACAGCGCTT
     5641 CCCAAACCTT TACCTTGGTG ATCTGGGCTG ACACCGACAG TGGCAAGAAA CCACGCAGGC
     5701 TCCTTTGGTC TATGAGGAGC CAGCAAGCCC TCCATTTGCT GTTGTGCCGC CAACCTACTC
     5761 CCTGACAGCT CGGCCATCCG CGGACCTATT TCCGCGAACA CTGCGCCGGC TTCGACGCTT
     5821 TCCGGAGTAG TCCAGACCGC AACTGCGGCA CCATCGTCCG CAACCCAAAC TTTACCAATG
     5881 TCCAGACCCA CGCGCGTAAG AAACAGCTCT TGCAACTCAG TTACCCTCTC TATGTGTCTG
     5941 TCCGGATCTA CGGTATGGCG GGTCGCTGGA TAATCTGCAA ATGCTGCGGC AAGTGTTCGG
     6001 ACAGCCCGTG GTACATCGTC ACGGGTAGCG AGCCTAACAG TGGGCTTATA TTCCGTCATG
     6061 GGGCCGGGGT TCTCCTCCAC GTCGCCGGCC TGCTTCAGCA GGCTGAAGTT GGTGGCGCCG
     6121 CTGCCCCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG CAGCATATCA
     6181 AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC GTCAAGGGCG
     6241 TCGGTCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT GATTAGTTCC
     6301 AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT TGCTTGTTTC
     6361 AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC TACTTGATGC
     6421 TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG AGCGTACAGT
     6481 GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC CAGGGTTTCG
     6541 TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCACG ATGTGAGAGG
     6601 AGAGCACAGC GGTATGACTT GGCGTTGTTC CGCAGAAAGT CTTGCCATGA CTCGCCTTCC
     6661 AGGGGGCAGG AGTGGGTATG ATGCCTGTCC AGCATCTCGA TTGGCAGGGC ATCGAGCAGG
     6721 GCCCGCTTGT TCTTCACGTG CCAGTACAGG GTAGGCTGCT CAACTCCCAG CTTTTGAGCG
     6781 AGTTTCCTTG TCGTCAGGCC TTCGATACCG ACTCCATTGA GTAATTCCAG AGCAGAGTTT
     6841 ATGACTTTGC TCTTGTCCAG TCTAGACATC TTATCGTCAT CGTCTTTGTA ATCCATGGTG
     6901 GCGGATCCCG CGTCACGACA CCTGTGTTCT GGCGGCAAAC CCGTTGCGAA AAAGAACGTT
     6961 CACGGCGACT ACTGCACTTA TATACGGTTC TCCCCCACCC TCGGGAAAAA GGCGGAGCCA
     7021 GTACACGACA TCACTTTCCC AGTTTACCCC GCGCCACCTT CTCTAGGCAC CGGTTCAATT
     7081 GCCGACCCCT CCCCCCAACT TCTCGGGCAC TGTGGGCCAT GTGCGCTCTG CCCCATGGTA
     7141 ATAGCCATGA CTAATACGTA GATGTACTGC CAAGTAGGAA AGTGCCATAA GGTCATGTAC
     7201 TGGGCATAAT GCCAGGCGGG CCATTTACCG TCATTGACGT CAATAGGGGG CGTACTTGGC
     7261 ATATGATACA CTTGATGTAC TGCCAAGTGG GCAGTTTACC GTAAATACTC CACCCATTGA
     7321 CGTCAATGGA AAGTGCCTAT TGGCGTTACT ATGGGAACAT ACGTCATTAT TGACGTCAAT
     7381 GGGCGGGGGT CGTTGGGCGG TCAGCCAGGC GGGCCATTTA CCGTAAGTTA TGTAACGCGG
     7441 AACTCCATAT ATGGGCTATG AACTAATGAC CCCGTAATTG ATTACTATTA ATAACTAGTC
     7501 AATAATCAAT GTCATAAGCT CTAATCGCAT AATCGAGCTA AGAGCGATTA ATATGCTTAG
     7561 CTAGCTTAGC TAAGCTAGAT TTCTCTATTC TCCCACAATA TATAGCAGCT ATTAGCTTAG
     7621 CAGTCCGAAT ACGATCGTCC ACGATCCATA AGCGTAACAC ATTTGAGTAG CGACTGACGG
     7681 GCACCGGAGC CACTCGAGTG GAATTACTGA CGGGCACCGG AGCCACTCGA GTGGAATT
//