LOCUS       CK530_18_ATF4           7564 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..391
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     392..1445
                      /label="ATF4"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              392..1507
                      /label="Translation 392-1507"
     misc_feature     complement(1449..1467)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1453..1469)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1453..1470)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1453..1533
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1458..1467
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1472..1498
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1499..1507
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      1511..1533
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      1511..1533
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 1534..1603
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1538..1560
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1561..1580
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1581..1603)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1604..1607
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1608..1630)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(1608..1630)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 1608..1639
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1631..1655
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1640..1673
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1665..1799
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1674..1829
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 1830..4540
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2071..2105)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2117..2132)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2341..2360)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2462..2484
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2523..2541)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2609..2713
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2714..3574
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2932..2951)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       3745..4333
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4234..4253
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4487..4504
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4541..4629
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4630..7498
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4710..4731
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4746..4776
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4784..4800
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4808..4824
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    4850..4912
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5088..5169
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5196..5215
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5286..5885)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5771..5790
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(5886..5942)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(5952..6695)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6093..6112)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6338..6357
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(6696..6719)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6719..6728)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6748..6959)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(6960..7339)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7488..7507
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7499..7531
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7532..7564
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7541..7559)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGTTGG CATGACCGAA ATGAGCTTCC TGAGCAGCGA
      421 GGTGTTGGTG GGGGACTTGA TGTCCCCCTT CGACCAGTCG GGTTTGGGGG CTGAAGAAAG
      481 CCTAGGTCTC TTAGATGATT ACCTGGAGGT GGCCAAGCAC TTCAAACCTC ATGGGTTCTC
      541 CAGCGACAAG GCTAAGGCGG GCTCCTCCGA ATGGCTGGCT GTGGATGGGT TGGTCAGTCC
      601 CTCCAACAAC AGCAAGGAGG ATGCCTTCTC CGGGACAGAT TGGATGTTGG AGAAAATGGA
      661 TTTGAAGGAG TTCGACTTGG ATGCCCTGTT GGGTATAGAT GACCTGGAAA CCATGCCAGA
      721 TGACCTTCTG ACCACGTTGG ATGACACTTG TGATCTCTTT GCCCCCCTAG TCCAGGAGAC
      781 TAATAAGCAG CCCCCCCAGA CGGTGAACCC AATTGGCCAT CTCCCAGAAA GTTTAACAAA
      841 ACCCGACCAG GTTGCCCCCT TCACCTTCTT ACAACCTCTT CCCCTTTCCC CAGGGGTCCT
      901 GTCCTCCACT CCAGATCATT CCTTTAGTTT AGAGCTGGGC AGTGAAGTGG ATATCACTGA
      961 AGGAGATAGG AAGCCAGACT ACACTGCTTA CGTTGCCATG ATCCCTCAGT GCATAAAGGA
     1021 GGAAGACACC CCTTCAGATA ATGATAGTGG CATCTGTATG AGCCCAGAGT CCTATCTGGG
     1081 GTCTCCTCAG CACAGCCCCT CTACCAGGGG CTCTCCAAAT AGGAGCCTCC CATCTCCAGG
     1141 TGTTCTCTGT GGGTCTGCCC GTCCCAAACC TTACGATCCT CCTGGAGAGA AGATGGTAGC
     1201 AGCAAAAGTA AAGGGTGAGA AACTGGATAA GAAGCTGAAA AAAATGGAGC AAAACAAGAC
     1261 AGCAGCCACT AGGTACCGCC AGAAGAAGAG GGCGGAGCAG GAGGCTCTTA CTGGTGAGTG
     1321 CAAAGAGCTG GAAAAGAAGA ACGAGGCTCT AAAAGAGAGG GCGGATTCCC TGGCCAAGGA
     1381 GATCCAGTAC CTGAAAGATT TGATAGAAGA GGTCCGCAAG GCAAGGGGGA AGAAAAGGGT
     1441 CCCCTACCCA GCTTTCTTGT ACAAAGTGGT ATACCCATAC GATGTTCCAG ATTACGCTTA
     1501 GTAATGAGAA ATACTCAGAA GATGTCACTC ACCATAGTCC GCAGTGAAAG ATAAATGATC
     1561 NNNNNNNNNN NNNNNNNNNN GTTTTAGAGC TAGAAATAGC AAGTAGGCCA CTGAGCAAGA
     1621 ACTATGAATG AGATCTTTAG ACATATTGCG GCCGCTTTCT CCGCGATCCA GACATGATAA
     1681 GATACATTGA TGAGTTTGGA CAAACCAAAA CTAGAATGCA GTGAAAAAAA TGCCTTATTT
     1741 GTGAAATTTG TGATGCTATT GCCTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
     1801 TCTATAACAA GAAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA
     1861 TATAATTATC GTATGAGTTA AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT
     1921 GACTCACGCG GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT
     1981 CACGGGAGCT CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT
     2041 TTAGAAAGAG AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC
     2101 TAGGGTTAAG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT
     2161 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA
     2221 GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT
     2281 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG
     2341 TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA
     2401 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCATGTC
     2461 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACACGC GAGTTCGAAA
     2521 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC
     2581 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
     2641 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
     2701 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
     2761 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
     2821 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
     2881 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
     2941 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
     3001 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
     3061 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     3121 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     3181 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     3241 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     3301 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     3361 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     3421 TGAGCGTGGT TCACGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     3481 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     3541 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
     3601 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
     3661 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
     3721 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     3781 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     3841 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     3901 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     3961 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     4021 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     4081 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     4141 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     4201 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     4261 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     4321 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     4381 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
     4441 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
     4501 CGAGGAAGCG GATGAGCGCC CAATACGCAA ACCGCCTCTC ACCGCGCGTT GGCCGATTCA
     4561 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAA
     4621 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
     4681 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC
     4741 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA
     4801 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGG TCGACTTAAC CCTAGAAAGA
     4861 TAATCATATT GTGACGTACG TTAAAGATAA TCATGCGTAA AATTGACGCA TGTGTTTTAT
     4921 CGGTCTGTAT ATCGAGGTTT ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA
     4981 CACTTACATA CTAATAATAA ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA
     5041 AAAAAACAAA AACTCAAAAT TTCTTCTATA AAGTAACAAA ACTTTTAGCA GTGAAAAAAA
     5101 TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT
     5161 AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG
     5221 GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCCTCTG
     5281 GAGATTCATG CGCCAGGTTT TCTGGTCATG CACCACGTTC GTGGGCCCTC AGGAACCTCC
     5341 ACGTCAGCGG TAACTGTGAA GCCCAGTCGC TCGTAAAAAG GAAGATTCCG GGGAGCACTG
     5401 GTTTCGAGGA ACGCCGGCAC GCCAGCCCGT TCCGCGGCCT CAACACCAGG AAGAACGACA
     5461 GCGCTTCCCA AACCTTTACC TTGGTGATCT GGGCTGACAC CGACAGTGGC AAGAAACCAC
     5521 GCAGGCTCCT TTGGTCTATG AGGAGCCAGC AAGCCCTCCA TTTGCTGTTG TGCCGCCAAC
     5581 CTACTCCCTG ACAGCTCGGC CATCCGCGGA CCTATTTCCG CGAACACTGC GCCGGCTTCG
     5641 ACGCTTTCCG GAGTAGTCCA GACCGCAACT GCGGCACCAT CGTCCGCAAC CCAAACTTTA
     5701 CCAATGTCCA GACCCACGCG CGTAAGAAAC AGCTCTTGCA ACTCAGTTAC CCTCTCTATG
     5761 TGTCTGTCCG GATCTACGGT ATGGCGGGTC GCTGGATAAT CTGCAAATGC TGCGGCAAGT
     5821 GTTCGGACAG CCCGTGGTAC ATCGTCACGG GTAGCGAGCC TAACAGTGGG CTTATATTCC
     5881 GTCATGGGGC CGGGGTTCTC CTCCACGTCG CCGGCCTGCT TCAGCAGGCT GAAGTTGGTG
     5941 GCGCCGCTGC CCCCGGGGAG CATGTCAAGG TCAAAATCGT CAAGAGCGTC AGCAGGCAGC
     6001 ATATCAAGGT CAAAGTCGTC AAGGGCATCG GCTGGGAGCA TGTCTAAGTC AAAATCGTCA
     6061 AGGGCGTCGG TCGGCCCGCC GCTTTCGCAC TTTAGCTGTT TCTCCAGGCC ACATATGATT
     6121 AGTTCCAGGC CGAAAAGGAA GGCAGGTTCG GCTCCCTGCC GGTCGAACAG CTCAATTGCT
     6181 TGTTTCAGAA GTGGGGGCAT AGAATCGGTG GTAGGTGTCT CTCTTTCCTC TTTTGCTACT
     6241 TGATGCTCCT GTTCCTCCAA TACGCAGCCC AGTGTAAAGT GGCCCACGGC GGACAGAGCG
     6301 TACAGTGCGT TCTCCAGGGA GAAGCCTTGC TGACACAGGA ACGCGAGCTG ATTTTCCAGG
     6361 GTTTCGTACT GTTTCTCTGT TGGGCGGGTG CCGAGATGCA CTTTAGCCCC GTCACGATGT
     6421 GAGAGGAGAG CACAGCGGTA TGACTTGGCG TTGTTCCGCA GAAAGTCTTG CCATGACTCG
     6481 CCTTCCAGGG GGCAGGAGTG GGTATGATGC CTGTCCAGCA TCTCGATTGG CAGGGCATCG
     6541 AGCAGGGCCC GCTTGTTCTT CACGTGCCAG TACAGGGTAG GCTGCTCAAC TCCCAGCTTT
     6601 TGAGCGAGTT TCCTTGTCGT CAGGCCTTCG ATACCGACTC CATTGAGTAA TTCCAGAGCA
     6661 GAGTTTATGA CTTTGCTCTT GTCCAGTCTA GACATCTTAT CGTCATCGTC TTTGTAATCC
     6721 ATGGTGGCGG ATCCCGCGTC ACGACACCTG TGTTCTGGCG GCAAACCCGT TGCGAAAAAG
     6781 AACGTTCACG GCGACTACTG CACTTATATA CGGTTCTCCC CCACCCTCGG GAAAAAGGCG
     6841 GAGCCAGTAC ACGACATCAC TTTCCCAGTT TACCCCGCGC CACCTTCTCT AGGCACCGGT
     6901 TCAATTGCCG ACCCCTCCCC CCAACTTCTC GGGCACTGTG GGCCATGTGC GCTCTGCCCC
     6961 ATGGTAATAG CCATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTG CCATAAGGTC
     7021 ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA
     7081 CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC
     7141 CATTGACGTC AATGGAAAGT GCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC
     7201 GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA
     7261 ACGCGGAACT CCATATATGG GCTATGAACT AATGACCCCG TAATTGATTA CTATTAATAA
     7321 CTAGTCAATA ATCAATGTCA TAAGCTCTAA TCGCATAATC GAGCTAAGAG CGATTAATAT
     7381 GCTTAGCTAG CTTAGCTAAG CTAGATTTCT CTATTCTCCC ACAATATATA GCAGCTATTA
     7441 GCTTAGCAGT CCGAATACGA TCGTCCACGA TCCATAAGCG TAACACATTT GAGTAGCGAC
     7501 TGACGGGCAC CGGAGCCACT CGAGTGGAAT TACTGACGGG CACCGGAGCC ACTCGAGTGG
     7561 AATT
//