LOCUS       CK530_20_EGR1           8140 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..391
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     392..2021
                      /label="EGR1"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     CDS              392..2083
                      /label="Translation 392-2083"
     misc_feature     complement(2025..2043)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(2029..2045)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(2029..2046)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 2029..2109
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     2034..2043
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2048..2074
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       2075..2083
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      2087..2109
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      2087..2109
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 2110..2179
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  2114..2136
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     2137..2156
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(2157..2179)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 2180..2183
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(2184..2206)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2184..2206)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 2184..2215
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            2207..2231
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 2216..2249
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     2241..2375
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 2250..2405
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2406..5116
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2647..2681)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2693..2708)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2917..2936)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      3038..3060
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(3099..3117)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         3185..3289
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              3290..4150
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3508..3527)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       4321..4909
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4810..4829
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      5063..5080
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 5117..5205
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 5206..8074
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     5286..5307
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         5322..5352
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     5360..5376
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      5384..5400
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5426..5488
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5664..5745
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5772..5791
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5862..6461)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      6347..6366
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6462..6518)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6528..7271)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6669..6688)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6914..6933
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(7272..7295)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(7295..7304)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(7324..7535)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7536..7915)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      8064..8083
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 8075..8107
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 8108..8140
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(8117..8135)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGTTGG CATGGCCGCG GCCAAGGCCG AGATGCAGCT
      421 GATGTCCCCG CTGCAGATCT CTGACCCGTT CGGATCCTTT CCTCACTCGC CCACCATGGA
      481 CAACTACCCT AAGCTGGAGG AGATGATGCT GCTGAGCAAC GGGGCTCCCC AGTTCCTCGG
      541 CGCCGCCGGG GCCCCAGAGG GCAGCGGCAG CAACAGCAGC AGCAGCAGCA GCGGGGGCGG
      601 TGGAGGCGGC GGGGGCGGCA GCAACAGCAG CAGCAGCAGC AGCACCTTCA ACCCTCAGGC
      661 GGACACGGGC GAGCAGCCCT ACGAGCACCT GACCGCAGAG TCTTTTCCTG ACATCTCTCT
      721 GAACAACGAG AAGGTGCTGG TGGAGACCAG TTACCCCAGC CAAACCACTC GACTGCCCCC
      781 CATCACCTAT ACTGGCCGCT TTTCCCTGGA GCCTGCACCC AACAGTGGCA ACACCTTGTG
      841 GCCCGAGCCC CTCTTCAGCT TGGTCAGTGG CCTAGTGAGC ATGACCAACC CACCGGCCTC
      901 CTCGTCCTCA GCACCATCTC CAGCGGCCTC CTCCGCCTCC GCCTCCCAGA GCCCACCCCT
      961 GAGCTGCGCA GTGCCATCCA ACGACAGCAG TCCCATTTAC TCAGCGGCAC CCACCTTCCC
     1021 CACGCCGAAC ACTGACATTT TCCCTGAGCC ACAAAGCCAG GCCTTCCCGG GCTCGGCAGG
     1081 GACAGCGCTC CAGTACCCGC CTCCTGCCTA CCCTGCCGCC AAGGGTGGCT TCCAGGTTCC
     1141 CATGATCCCC GACTACCTGT TTCCACAGCA GCAGGGGGAT CTGGGCCTGG GCACCCCAGA
     1201 CCAGAAGCCC TTCCAGGGCC TGGAGAGCCG CACCCAGCAG CCTTCGCTAA CCCCTCTGTC
     1261 TACTATTAAG GCCTTTGCCA CTCAGTCGGG CTCCCAGGAC CTGAAGGCCC TCAATACCAG
     1321 CTACCAGTCC CAGCTCATCA AACCCAGCCG CATGCGCAAG TACCCCAACC GGCCCAGCAA
     1381 GACGCCCCCC CACGAACGCC CTTACGCTTG CCCAGTGGAG TCCTGTGATC GCCGCTTCTC
     1441 CCGCTCCGAC GAGCTCACCC GCCACATCCG CATCCACACA GGCCAGAAGC CCTTCCAGTG
     1501 CCGCATCTGC ATGCGCAACT TCAGCCGCAG CGACCACCTC ACCACCCACA TCCGCACCCA
     1561 CACAGGCGAA AAGCCCTTCG CCTGCGACAT CTGTGGAAGA AAGTTTGCCA GGAGCGATGA
     1621 ACGCAAGAGG CATACCAAGA TCCACTTGCG GCAGAAGGAC AAGAAAGCAG ACAAAAGTGT
     1681 TGTGGCCTCT TCGGCCACCT CCTCTCTCTC TTCCTACCCG TCCCCGGTTG CTACCTCTTA
     1741 CCCGTCCCCG GTTACTACCT CTTATCCATC CCCGGCCACC ACCTCATACC CATCCCCTGT
     1801 GCCCACCTCC TTCTCCTCTC CCGGCTCCTC GACCTACCCA TCCCCTGTGC ACAGTGGCTT
     1861 CCCCTCCCCG TCGGTGGCCA CCACGTACTC CTCTGTTCCC CCTGCTTTCC CGGCCCAGGT
     1921 CAGCAGCTTC CCTTCCTCAG CTGTCACCAA CTCCTTCAGC GCCTCCACAG GGCTTTCGGA
     1981 CATGACAGCA ACCTTTTCTC CCAGGACAAT TGAAATTTGC TACCCAGCTT TCTTGTACAA
     2041 AGTGGTATAC CCATACGATG TTCCAGATTA CGCTTAGTAA TGAGAAATAC TCAGAAGATG
     2101 TCACTCACCA TAGTCCGCAG TGAAAGATAA ATGATCNNNN NNNNNNNNNN NNNNNNGTTT
     2161 TAGAGCTAGA AATAGCAAGT AGGCCACTGA GCAAGAACTA TGAATGAGAT CTTTAGACAT
     2221 ATTGCGGCCG CTTTCTCCGC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
     2281 CCAAAACTAG AATGCAGTGA AAAAAATGCC TTATTTGTGA AATTTGTGAT GCTATTGCCT
     2341 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTATCTA TAACAAGAAA ATATATATAT
     2401 AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC
     2461 TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA
     2521 AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG
     2581 AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA
     2641 AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAAGAATT CACTGGCCGT
     2701 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
     2761 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
     2821 ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT
     2881 GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
     2941 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT
     3001 CCCGGCATCC GCTTACAGAC AAGCTGTGAC CATGTCTCCG GGAGCTGCAT GTGTCAGAGG
     3061 TTTTCACCGT CATCACCGAA ACACGCGAGT TCGAAAGGGC CTCGTGATAC GCCTATTTTT
     3121 ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA
     3181 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     3241 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA
     3301 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA
     3361 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA
     3421 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT
     3481 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC
     3541 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC
     3601 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC
     3661 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA
     3721 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA
     3781 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT
     3841 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA
     3901 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC
     3961 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGTTCAC GCGGTATCAT
     4021 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG
     4081 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
     4141 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     4201 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     4261 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     4321 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     4381 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     4441 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     4501 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     4561 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     4621 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     4681 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
     4741 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     4801 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
     4861 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
     4921 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
     4981 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
     5041 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGATG AGCGCCCAAT
     5101 ACGCAAACCG CCTCTCACCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
     5161 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
     5221 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
     5281 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
     5341 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
     5401 CATGATTACG CCAAGGTCGA CTTAACCCTA GAAAGATAAT CATATTGTGA CGTACGTTAA
     5461 AGATAATCAT GCGTAAAATT GACGCATGTG TTTTATCGGT CTGTATATCG AGGTTTATTT
     5521 ATTAATTTGA ATAGATATTA AGTTTTATTA TATTTACACT TACATACTAA TAATAAATTC
     5581 AACAAACAAT TTATTTATGT TTATTTATTT ATTAAAAAAA AACAAAAACT CAAAATTTCT
     5641 TCTATAAAGT AACAAAACTT TTAGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT
     5701 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC
     5761 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC
     5821 CTCTACAAAT GTGGTATGGC TGATTATGAT CCTCTGGAGA TTCATGCGCC AGGTTTTCTG
     5881 GTCATGCACC ACGTTCGTGG GCCCTCAGGA ACCTCCACGT CAGCGGTAAC TGTGAAGCCC
     5941 AGTCGCTCGT AAAAAGGAAG ATTCCGGGGA GCACTGGTTT CGAGGAACGC CGGCACGCCA
     6001 GCCCGTTCCG CGGCCTCAAC ACCAGGAAGA ACGACAGCGC TTCCCAAACC TTTACCTTGG
     6061 TGATCTGGGC TGACACCGAC AGTGGCAAGA AACCACGCAG GCTCCTTTGG TCTATGAGGA
     6121 GCCAGCAAGC CCTCCATTTG CTGTTGTGCC GCCAACCTAC TCCCTGACAG CTCGGCCATC
     6181 CGCGGACCTA TTTCCGCGAA CACTGCGCCG GCTTCGACGC TTTCCGGAGT AGTCCAGACC
     6241 GCAACTGCGG CACCATCGTC CGCAACCCAA ACTTTACCAA TGTCCAGACC CACGCGCGTA
     6301 AGAAACAGCT CTTGCAACTC AGTTACCCTC TCTATGTGTC TGTCCGGATC TACGGTATGG
     6361 CGGGTCGCTG GATAATCTGC AAATGCTGCG GCAAGTGTTC GGACAGCCCG TGGTACATCG
     6421 TCACGGGTAG CGAGCCTAAC AGTGGGCTTA TATTCCGTCA TGGGGCCGGG GTTCTCCTCC
     6481 ACGTCGCCGG CCTGCTTCAG CAGGCTGAAG TTGGTGGCGC CGCTGCCCCC GGGGAGCATG
     6541 TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA GTCGTCAAGG
     6601 GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT
     6661 TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA AAGGAAGGCA
     6721 GGTTCGGCTC CCTGCCGGTC GAACAGCTCA ATTGCTTGTT TCAGAAGTGG GGGCATAGAA
     6781 TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGTTC CTCCAATACG
     6841 CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC CAGGGAGAAG
     6901 CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT CTCTGTTGGG
     6961 CGGGTGCCGA GATGCACTTT AGCCCCGTCA CGATGTGAGA GGAGAGCACA GCGGTATGAC
     7021 TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA GGAGTGGGTA
     7081 TGATGCCTGT CCAGCATCTC GATTGGCAGG GCATCGAGCA GGGCCCGCTT GTTCTTCACG
     7141 TGCCAGTACA GGGTAGGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG
     7201 CCTTCGATAC CGACTCCATT GAGTAATTCC AGAGCAGAGT TTATGACTTT GCTCTTGTCC
     7261 AGTCTAGACA TCTTATCGTC ATCGTCTTTG TAATCCATGG TGGCGGATCC CGCGTCACGA
     7321 CACCTGTGTT CTGGCGGCAA ACCCGTTGCG AAAAAGAACG TTCACGGCGA CTACTGCACT
     7381 TATATACGGT TCTCCCCCAC CCTCGGGAAA AAGGCGGAGC CAGTACACGA CATCACTTTC
     7441 CCAGTTTACC CCGCGCCACC TTCTCTAGGC ACCGGTTCAA TTGCCGACCC CTCCCCCCAA
     7501 CTTCTCGGGC ACTGTGGGCC ATGTGCGCTC TGCCCCATGG TAATAGCCAT GACTAATACG
     7561 TAGATGTACT GCCAAGTAGG AAAGTGCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG
     7621 GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT
     7681 ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTGCCT
     7741 ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC
     7801 GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA
     7861 TGAACTAATG ACCCCGTAAT TGATTACTAT TAATAACTAG TCAATAATCA ATGTCATAAG
     7921 CTCTAATCGC ATAATCGAGC TAAGAGCGAT TAATATGCTT AGCTAGCTTA GCTAAGCTAG
     7981 ATTTCTCTAT TCTCCCACAA TATATAGCAG CTATTAGCTT AGCAGTCCGA ATACGATCGT
     8041 CCACGATCCA TAAGCGTAAC ACATTTGAGT AGCGACTGAC GGGCACCGGA GCCACTCGAG
     8101 TGGAATTACT GACGGGCACC GGAGCCACTC GAGTGGAATT
//