LOCUS       CK530_21_FOXL2          7648 bp ds-DNA     circular     25-JUN-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           322..342
                      /label="mCMV_seq"
                      /note="sequence: GCTGTTTTGACCTCCATAGAA"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..398
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     399..1528
                      /label="FOXL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer           401..420
                      /label="MPS127_FOXL2_5'_outer"
                      /note="sequence: GATGGCCAGCTACCCCGAGC"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer           1424..1442
                      /label="MPS176"
                      /note="sequence: CCTGCAGTTCGCTTGTGCC"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     complement(1533..1551)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1537..1553)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1537..1554)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1537..1617
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1542..1551
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1556..1582
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1583..1591
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer           1589..1617
                      /label="MPS311_BC_fwd2"
                      /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer           complement(1592..1617)
                      /label="MPS312-gBlock_attR2-DD_rev"
                      /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     primer_bind      1595..1617
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      1595..1617
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer           1595..1617
                      /label="MPS287-barcode-fwd"
                      /note="sequence: ATACTCAGAAGATGTCACTCACC"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 1618..1687
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1622..1644
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1645..1664
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1665..1687)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1688..1691
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1692..1714)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1692..1714)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer           complement(1692..1714)
                      /label="MPS288-barcode-rev"
                      /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 1692..1723
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1715..1739
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1724..1757
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           complement(1745..1766)
                      /label="MPS294_barcode_rev2"
                      /note="sequence: TCTTATCATGTCTGGATCGCGG"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal     1749..1883
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1758..1913
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 1914..4624
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2155..2189)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2201..2216)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2425..2444)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2546..2568
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2607..2625)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           2681..2700
                      /label="MPS112_AmpR_prom_fwd"
                      /note="sequence: cggggaaatgtgcgcggaac"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     promoter         2693..2797
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2798..3658
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3016..3035)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer           complement(3016..3035)
                      /label="MPS101_Amp_rev"
                      /note="sequence: ataataccgcgccacatagc"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     rep_origin       3829..4417
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4318..4337
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4571..4588
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4625..4713
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4714..7582
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4794..4815
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4830..4860
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4868..4884
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4892..4908
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    4934..4996
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5172..5253
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5280..5299
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           complement(5346..5369)
                      /label="MPS318_resistance_rev"
                      /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              complement(5370..5969)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5855..5874
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     primer           5970..5987
                      /label="MPS315_P2A-rev"
                      /note="sequence: GGGGCCGGGGTTCTCCTC"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(5970..6026)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6036..6779)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6177..6196)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer           6380..6399
                      /label="qMPS015F"
                      /note="sequence: GAGCGTACAGTGCGTTCTCC"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     primer_bind      6422..6441
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer           complement(6476..6495)
                      /label="qMPS015R"
                      /note="sequence: CGGGGCTAAAGTGCATCTCG"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     CDS              complement(6780..6803)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6803..6812)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6832..7043)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7044..7423)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7572..7591
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7583..7615
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7616..7648
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7625..7643)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTGCCACCAT GATGGCCAGC TACCCCGAGC
      421 CCGAGGACGC GGCGGGGGCC CTGCTGGCCC CAGAGACCGG TCGCACAGTC AAGGAGCCAG
      481 AAGGGCCGCC GCCGAGCCCA GGCAAGGGCG GTGGGGGTGG CGGCGGGACA GCCCCGGAGA
      541 AGCCGGACCC GGCGCAGAAG CCCCCGTACT CGTACGTGGC GCTCATCGCC ATGGCGATCC
      601 GCGAGAGCGC GGAGAAGAGG CTCACGCTGT CCGGCATCTA CCAGTACATC ATCGCGAAGT
      661 TCCCGTTCTA CGAGAAGAAT AAGAAGGGCT GGCAAAATAG CATCCGCCAC AACCTCAGCC
      721 TCAACGAGTG CTTCATCAAG GTGCCGCGCG AGGGCGGCGG CGAGCGCAAG GGCAACTACT
      781 GGACGCTGGA CCCGGCCTGC GAAGACATGT TCGAGAAGGG CAACTACCGG CGCCGCCGCC
      841 GCATGAAGAG GCCCTTCCGG CCGCCGCCCG CGCACTTCCA GCCCGGCAAG GGGCTCTTCG
      901 GGGCCGGAGG CGCCGCAGGC GGGTGCGGCG TGGCGGGCGC CGGGGCCGAC GGCTACGGCT
      961 ACCTGGCGCC CCCCAAGTAC CTGCAGTCTG GCTTCCTCAA CAACTCGTGG CCGCTACCGC
     1021 AGCCTCCCTC ACCCATGCCC TATGCCTCCT GCCAGATGGC GGCAGCCGCA GCGGCTGCAG
     1081 CAGCTGCGGC TGCAGCCGCG GGCCCCGGTA GCCCTGGCGC GGCCGCTGTG GTCAAGGGGC
     1141 TGGCGGGCCC GGCCGCCTCG TACGGGCCGT ACACACGCGT GCAGAGCATG GCGCTGCCCC
     1201 CCGGCGTAGT GAACTCGTAC AATGGCCTGG GAGGCCCGCC GGCCGCACCC CCGCCTCCGC
     1261 CGCACCCCCA CCCGCATCCG CACGCACACC ATCTGCACGC GGCCGCCGCA CCGCCGCCTG
     1321 CCCCACCGCA CCACGGGGCC GCCGCGCCGC CGCCGGGCCA GCTCAGCCCT GCCAGCCCAG
     1381 CCACCGCCGC GCCCCCGGCG CCCGCGCCCA CCAGTGCGCC GGGCCTGCAG TTCGCTTGTG
     1441 CCCGGCAGCC CGAGCTCGCC ATGATGCATT GCTCTTACTG GGACCACGAC AGCAAGACCG
     1501 GCGCGCTGCA TTCGCGCCTC GATCTCTGAA CCCAGCTTTC TTGTACAAAG TGGTATACCC
     1561 ATACGATGTT CCAGATTACG CTTAGTAATG AGAAATACTC AGAAGATGTC ACTCACCATA
     1621 GTCCGCAGTG AAAGATAAAT GATCNNNNNN NNNNNNNNNN NNNNGTTTTA GAGCTAGAAA
     1681 TAGCAAGTAG GCCACTGAGC AAGAACTATG AATGAGATCT TTAGACATAT TGCGGCCGCT
     1741 TTCTCCGCGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC AAAACTAGAA
     1801 TGCAGTGAAA AAAATGCCTT ATTTGTGAAA TTTGTGATGC TATTGCCTTA TTTGTAACCA
     1861 TTATAAGCTG CAATAAACAA GTTATCTATA ACAAGAAAAT ATATATATAA TAAGTTATCA
     1921 CGTAAGTAGA ACATGAAATA ACAATATAAT TATCGTATGA GTTAAATCTT AAAAGTCACG
     1981 TAAAAGATAA TCATGCGTCA TTTTGACTCA CGCGGTCGTT ATAGTTCAAA ATCAGTGACA
     2041 CTTACCGCAT TGACAAGCAC GCCTCACGGG AGCTCCAAGC GGCGACTGAG ATGTCCTAAA
     2101 TGCACAGCGA CGGATTCGCG CTATTTAGAA AGAGAGAGCA ATATTTCAAG AATGCATGCG
     2161 TCAATTTTAC GCAGACTATC TTTCTAGGGT TAAGAATTCA CTGGCCGTCG TTTTACAACG
     2221 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
     2281 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
     2341 CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
     2401 ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC
     2461 CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC
     2521 TTACAGACAA GCTGTGACCA TGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA
     2581 TCACCGAAAC ACGCGAGTTC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT
     2641 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
     2701 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
     2761 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
     2821 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
     2881 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
     2941 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
     3001 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
     3061 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
     3121 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
     3181 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
     3241 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
     3301 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT
     3361 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
     3421 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
     3481 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGTTCACGC GGTATCATTG CAGCACTGGG
     3541 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
     3601 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT
     3661 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
     3721 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
     3781 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     3841 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     3901 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     3961 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     4021 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     4081 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     4141 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     4201 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     4261 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     4321 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     4381 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     4441 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
     4501 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
     4561 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGATGAG CGCCCAATAC GCAAACCGCC
     4621 TCTCACCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA
     4681 AGCGGGCAGT GAGCGCAACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC
     4741 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG
     4801 AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG
     4861 TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC
     4921 AAGGTCGACT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC
     4981 GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT
     5041 AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT
     5101 ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA
     5161 CAAAACTTTT AGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
     5221 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
     5281 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
     5341 GGTATGGCTG ATTATGATCC TCTGGAGATT CATGCGCCAG GTTTTCTGGT CATGCACCAC
     5401 GTTCGTGGGC CCTCAGGAAC CTCCACGTCA GCGGTAACTG TGAAGCCCAG TCGCTCGTAA
     5461 AAAGGAAGAT TCCGGGGAGC ACTGGTTTCG AGGAACGCCG GCACGCCAGC CCGTTCCGCG
     5521 GCCTCAACAC CAGGAAGAAC GACAGCGCTT CCCAAACCTT TACCTTGGTG ATCTGGGCTG
     5581 ACACCGACAG TGGCAAGAAA CCACGCAGGC TCCTTTGGTC TATGAGGAGC CAGCAAGCCC
     5641 TCCATTTGCT GTTGTGCCGC CAACCTACTC CCTGACAGCT CGGCCATCCG CGGACCTATT
     5701 TCCGCGAACA CTGCGCCGGC TTCGACGCTT TCCGGAGTAG TCCAGACCGC AACTGCGGCA
     5761 CCATCGTCCG CAACCCAAAC TTTACCAATG TCCAGACCCA CGCGCGTAAG AAACAGCTCT
     5821 TGCAACTCAG TTACCCTCTC TATGTGTCTG TCCGGATCTA CGGTATGGCG GGTCGCTGGA
     5881 TAATCTGCAA ATGCTGCGGC AAGTGTTCGG ACAGCCCGTG GTACATCGTC ACGGGTAGCG
     5941 AGCCTAACAG TGGGCTTATA TTCCGTCATG GGGCCGGGGT TCTCCTCCAC GTCGCCGGCC
     6001 TGCTTCAGCA GGCTGAAGTT GGTGGCGCCG CTGCCCCCGG GGAGCATGTC AAGGTCAAAA
     6061 TCGTCAAGAG CGTCAGCAGG CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG
     6121 AGCATGTCTA AGTCAAAATC GTCAAGGGCG TCGGTCGGCC CGCCGCTTTC GCACTTTAGC
     6181 TGTTTCTCCA GGCCACATAT GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC
     6241 TGCCGGTCGA ACAGCTCAAT TGCTTGTTTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT
     6301 GTCTCTCTTT CCTCTTTTGC TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA
     6361 AAGTGGCCCA CGGCGGACAG AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC
     6421 AGGAACGCGA GCTGATTTTC CAGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA
     6481 TGCACTTTAG CCCCGTCACG ATGTGAGAGG AGAGCACAGC GGTATGACTT GGCGTTGTTC
     6541 CGCAGAAAGT CTTGCCATGA CTCGCCTTCC AGGGGGCAGG AGTGGGTATG ATGCCTGTCC
     6601 AGCATCTCGA TTGGCAGGGC ATCGAGCAGG GCCCGCTTGT TCTTCACGTG CCAGTACAGG
     6661 GTAGGCTGCT CAACTCCCAG CTTTTGAGCG AGTTTCCTTG TCGTCAGGCC TTCGATACCG
     6721 ACTCCATTGA GTAATTCCAG AGCAGAGTTT ATGACTTTGC TCTTGTCCAG TCTAGACATC
     6781 TTATCGTCAT CGTCTTTGTA ATCCATGGTG GCGGATCCCG CGTCACGACA CCTGTGTTCT
     6841 GGCGGCAAAC CCGTTGCGAA AAAGAACGTT CACGGCGACT ACTGCACTTA TATACGGTTC
     6901 TCCCCCACCC TCGGGAAAAA GGCGGAGCCA GTACACGACA TCACTTTCCC AGTTTACCCC
     6961 GCGCCACCTT CTCTAGGCAC CGGTTCAATT GCCGACCCCT CCCCCCAACT TCTCGGGCAC
     7021 TGTGGGCCAT GTGCGCTCTG CCCCATGGTA ATAGCCATGA CTAATACGTA GATGTACTGC
     7081 CAAGTAGGAA AGTGCCATAA GGTCATGTAC TGGGCATAAT GCCAGGCGGG CCATTTACCG
     7141 TCATTGACGT CAATAGGGGG CGTACTTGGC ATATGATACA CTTGATGTAC TGCCAAGTGG
     7201 GCAGTTTACC GTAAATACTC CACCCATTGA CGTCAATGGA AAGTGCCTAT TGGCGTTACT
     7261 ATGGGAACAT ACGTCATTAT TGACGTCAAT GGGCGGGGGT CGTTGGGCGG TCAGCCAGGC
     7321 GGGCCATTTA CCGTAAGTTA TGTAACGCGG AACTCCATAT ATGGGCTATG AACTAATGAC
     7381 CCCGTAATTG ATTACTATTA ATAACTAGTC AATAATCAAT GTCATAAGCT CTAATCGCAT
     7441 AATCGAGCTA AGAGCGATTA ATATGCTTAG CTAGCTTAGC TAAGCTAGAT TTCTCTATTC
     7501 TCCCACAATA TATAGCAGCT ATTAGCTTAG CAGTCCGAAT ACGATCGTCC ACGATCCATA
     7561 AGCGTAACAC ATTTGAGTAG CGACTGACGG GCACCGGAGC CACTCGAGTG GAATTACTGA
     7621 CGGGCACCGG AGCCACTCGA GTGGAATT
//