LOCUS       CK530_22_NR5A1          7894 bp ds-DNA     circular     01-JUL-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..391
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     392..1776
                      /label="NR5A1"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     CDS              392..1837
                      /label="Translation 392-1837"
     primer           1482..1500
                      /label="qMPS31F"
                      /note="sequence: ACCGGCAGGAGTTTGTCTG"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature     complement(1779..1797)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1783..1799)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1783..1800)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1783..1863
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1788..1797
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1802..1828
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1829..1837
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      1841..1863
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      1841..1863
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1864..1933
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1868..1890
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1891..1910
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1911..1933)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1934..1937
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1938..1960)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(1938..1960)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 1938..1969
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1961..1985
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1970..2003
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1995..2129
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 2004..2159
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2160..4870
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2401..2435)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2447..2462)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2671..2690)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2792..2814
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2853..2871)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2939..3043
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              3044..3904
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3262..3281)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       4075..4663
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4564..4583
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4817..4834
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4871..4959
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4960..7828
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     5040..5061
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         5076..5106
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     5114..5130
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      5138..5154
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5180..5242
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5418..5499
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5526..5545
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5616..6215)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      6101..6120
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6216..6272)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6282..7025)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6423..6442)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6668..6687
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(7026..7049)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(7049..7058)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(7078..7289)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7290..7669)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7818..7837
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7829..7861
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7862..7894
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7871..7889)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGTTGG CATGGACTAT TCGTACGACG AGGACCTGGA
      421 CGAGCTGTGC CCCGTGTGCG GGGACAAGGT GTCCGGCTAC CACTACGGAC TGCTCACGTG
      481 TGAGAGCTGC AAGGGCTTCT TCAAGCGCAC GGTGCAGAAC AACAAGCACT ACACGTGCAC
      541 CGAGAGCCAG AGCTGCAAGA TCGACAAGAC GCAGCGCAAG CGCTGTCCCT TCTGCCGCTT
      601 CCAGAAATGC CTGACGGTGG GGATGCGCCT GGAAGCCGTG CGCGCTGACC GTATGAGGGG
      661 TGGCCGGAAC AAGTTTGGGC CGATGTACAA GCGGGACCGG GCCCTGAAAC AGCAGAAGAA
      721 GGCACAGATT CGGGCCAATG GCTTCAAGCT GGAGACAGGG CCCCCGATGG GGGTGCCCCC
      781 GCCGCCCCCT CCCGCACCGG ACTACGTGCT GCCTCCCAGC CTGCATGGGC CTGAGCCCAA
      841 GGGCCTGGCC GCCGGTCCAC CTGCTGGGCC ACTGGGCGAC TTTGGGGCCC CAGCACTGCC
      901 CATGGCCGTG CCCGGTGCCC ACGGGCCACT GGCTGGCTAC CTCTACCCTG CCTTTCCTGG
      961 CCGTGCCATC AAGTCTGAGT ACCCGGAGCC TTATGCCAGC CCCCCACAGC CTGGGCTGCC
     1021 GTACGGCTAC CCAGAGCCCT TCTCTGGAGG GCCCAACGTG CCTGAGCTCA TCCTGCAGCT
     1081 GCTGCAGCTG GAGCCGGATG AGGACCAGGT GCGGGCCCGC ATCTTGGGCT GCCTGCAGGA
     1141 GCCCACCAAA AGCCGCCCCG ACCAGCCGGC GGCCTTCGGC CTCCTGTGCA GAATGGCCGA
     1201 CCAGACCTTC ATCTCCATCG TGGACTGGGC ACGCAGGTGC ATGGTCTTCA AGGAGCTGGA
     1261 GGTGGCCGAC CAGATGACGC TGCTGCAGAA CTGCTGGAGC GAGCTGCTGG TGTTCGACCA
     1321 CATCTACCGC CAGGTCCAGC ACGGCAAGGA GGGCAGCATC CTGCTGGTCA CCGGGCAGGA
     1381 GGTGGAGCTG ACCACAGTGG CCACCCAGGC GGGCTCGCTG CTGCACAGCC TGGTGTTGCG
     1441 GGCGCAGGAG CTGGTGCTGC AGCTGCTTGC GCTGCAGCTG GACCGGCAGG AGTTTGTCTG
     1501 CCTCAAGTTC ATCATCCTCT TCAGCCTGGA TTTGAAGTTC CTGAATAACC ACATCCTGGT
     1561 GAAAGACGCT CAGGAGAAGG CCAACGCCGC CCTGCTTGAC TACACCCTGT GCCACTACCC
     1621 GCACTGCGGG GACAAATTCC AGCAGCTGCT GCTGTGCCTG GTGGAGGTGC GGGCCCTGAG
     1681 CATGCAGGCC AAGGAGTACC TGTACCACAA GCACCTGGGC AACGAGATGC CCCGCAACAA
     1741 CCTGCTCATC GAAATGCTGC AAGCCAAGCA GACTTGCCCA GCTTTCTTGT ACAAAGTGGT
     1801 ATACCCATAC GATGTTCCAG ATTACGCTTA GTAATGAGAA ATACTCAGAA GATGTCACTC
     1861 ACCATAGTCC GCAGTGAAAG ATAAATGATC NNNNNNNNNN NNNNNNNNNN GTTTTAGAGC
     1921 TAGAAATAGC AAGTAGGCCA CTGAGCAAGA ACTATGAATG AGATCTTTAG ACATATTGCG
     1981 GCCGCTTTCT CCGCGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCAAAA
     2041 CTAGAATGCA GTGAAAAAAA TGCCTTATTT GTGAAATTTG TGATGCTATT GCCTTATTTG
     2101 TAACCATTAT AAGCTGCAAT AAACAAGTTA TCTATAACAA GAAAATATAT ATATAATAAG
     2161 TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA AATCTTAAAA
     2221 GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG GTCGTTATAG TTCAAAATCA
     2281 GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT CCAAGCGGCG ACTGAGATGT
     2341 CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG AGAGCAATAT TTCAAGAATG
     2401 CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAG AATTCACTGG CCGTCGTTTT
     2461 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
     2521 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT
     2581 GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
     2641 TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG
     2701 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC
     2761 ATCCGCTTAC AGACAAGCTG TGACCATGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA
     2821 CCGTCATCAC CGAAACACGC GAGTTCGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT
     2881 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG
     2941 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA
     3001 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT
     3061 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA
     3121 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA
     3181 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT
     3241 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA
     3301 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT
     3361 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC
     3421 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT
     3481 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA
     3541 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC
     3601 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT
     3661 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG
     3721 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGT TCACGCGGTA TCATTGCAGC
     3781 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC
     3841 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG
     3901 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
     3961 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
     4021 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     4081 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     4141 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     4201 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     4261 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     4321 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     4381 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     4441 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     4501 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     4561 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     4621 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     4681 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC
     4741 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG
     4801 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GATGAGCGCC CAATACGCAA
     4861 ACCGCCTCTC ACCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
     4921 CTGGAAAGCG GGCAGTGAGC GCAACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
     4981 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT
     5041 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT
     5101 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT
     5161 TACGCCAAGG TCGACTTAAC CCTAGAAAGA TAATCATATT GTGACGTACG TTAAAGATAA
     5221 TCATGCGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT ATTTATTAAT
     5281 TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA ATTCAACAAA
     5341 CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAAACAAA AACTCAAAAT TTCTTCTATA
     5401 AAGTAACAAA ACTTTTAGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT
     5461 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT
     5521 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC
     5581 AAATGTGGTA TGGCTGATTA TGATCCTCTG GAGATTCATG CGCCAGGTTT TCTGGTCATG
     5641 CACCACGTTC GTGGGCCCTC AGGAACCTCC ACGTCAGCGG TAACTGTGAA GCCCAGTCGC
     5701 TCGTAAAAAG GAAGATTCCG GGGAGCACTG GTTTCGAGGA ACGCCGGCAC GCCAGCCCGT
     5761 TCCGCGGCCT CAACACCAGG AAGAACGACA GCGCTTCCCA AACCTTTACC TTGGTGATCT
     5821 GGGCTGACAC CGACAGTGGC AAGAAACCAC GCAGGCTCCT TTGGTCTATG AGGAGCCAGC
     5881 AAGCCCTCCA TTTGCTGTTG TGCCGCCAAC CTACTCCCTG ACAGCTCGGC CATCCGCGGA
     5941 CCTATTTCCG CGAACACTGC GCCGGCTTCG ACGCTTTCCG GAGTAGTCCA GACCGCAACT
     6001 GCGGCACCAT CGTCCGCAAC CCAAACTTTA CCAATGTCCA GACCCACGCG CGTAAGAAAC
     6061 AGCTCTTGCA ACTCAGTTAC CCTCTCTATG TGTCTGTCCG GATCTACGGT ATGGCGGGTC
     6121 GCTGGATAAT CTGCAAATGC TGCGGCAAGT GTTCGGACAG CCCGTGGTAC ATCGTCACGG
     6181 GTAGCGAGCC TAACAGTGGG CTTATATTCC GTCATGGGGC CGGGGTTCTC CTCCACGTCG
     6241 CCGGCCTGCT TCAGCAGGCT GAAGTTGGTG GCGCCGCTGC CCCCGGGGAG CATGTCAAGG
     6301 TCAAAATCGT CAAGAGCGTC AGCAGGCAGC ATATCAAGGT CAAAGTCGTC AAGGGCATCG
     6361 GCTGGGAGCA TGTCTAAGTC AAAATCGTCA AGGGCGTCGG TCGGCCCGCC GCTTTCGCAC
     6421 TTTAGCTGTT TCTCCAGGCC ACATATGATT AGTTCCAGGC CGAAAAGGAA GGCAGGTTCG
     6481 GCTCCCTGCC GGTCGAACAG CTCAATTGCT TGTTTCAGAA GTGGGGGCAT AGAATCGGTG
     6541 GTAGGTGTCT CTCTTTCCTC TTTTGCTACT TGATGCTCCT GTTCCTCCAA TACGCAGCCC
     6601 AGTGTAAAGT GGCCCACGGC GGACAGAGCG TACAGTGCGT TCTCCAGGGA GAAGCCTTGC
     6661 TGACACAGGA ACGCGAGCTG ATTTTCCAGG GTTTCGTACT GTTTCTCTGT TGGGCGGGTG
     6721 CCGAGATGCA CTTTAGCCCC GTCACGATGT GAGAGGAGAG CACAGCGGTA TGACTTGGCG
     6781 TTGTTCCGCA GAAAGTCTTG CCATGACTCG CCTTCCAGGG GGCAGGAGTG GGTATGATGC
     6841 CTGTCCAGCA TCTCGATTGG CAGGGCATCG AGCAGGGCCC GCTTGTTCTT CACGTGCCAG
     6901 TACAGGGTAG GCTGCTCAAC TCCCAGCTTT TGAGCGAGTT TCCTTGTCGT CAGGCCTTCG
     6961 ATACCGACTC CATTGAGTAA TTCCAGAGCA GAGTTTATGA CTTTGCTCTT GTCCAGTCTA
     7021 GACATCTTAT CGTCATCGTC TTTGTAATCC ATGGTGGCGG ATCCCGCGTC ACGACACCTG
     7081 TGTTCTGGCG GCAAACCCGT TGCGAAAAAG AACGTTCACG GCGACTACTG CACTTATATA
     7141 CGGTTCTCCC CCACCCTCGG GAAAAAGGCG GAGCCAGTAC ACGACATCAC TTTCCCAGTT
     7201 TACCCCGCGC CACCTTCTCT AGGCACCGGT TCAATTGCCG ACCCCTCCCC CCAACTTCTC
     7261 GGGCACTGTG GGCCATGTGC GCTCTGCCCC ATGGTAATAG CCATGACTAA TACGTAGATG
     7321 TACTGCCAAG TAGGAAAGTG CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT
     7381 TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC
     7441 AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT GCCTATTGGC
     7501 GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG
     7561 CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT
     7621 AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA ATCAATGTCA TAAGCTCTAA
     7681 TCGCATAATC GAGCTAAGAG CGATTAATAT GCTTAGCTAG CTTAGCTAAG CTAGATTTCT
     7741 CTATTCTCCC ACAATATATA GCAGCTATTA GCTTAGCAGT CCGAATACGA TCGTCCACGA
     7801 TCCATAAGCG TAACACATTT GAGTAGCGAC TGACGGGCAC CGGAGCCACT CGAGTGGAAT
     7861 TACTGACGGG CACCGGAGCC ACTCGAGTGG AATT
//