LOCUS       CK530_24_MAFF           7003 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..391
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     392..884
                      /label="MAFF"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              392..946
                      /label="Translation 392-946"
     misc_feature     complement(888..906)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(892..908)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(892..909)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 892..972
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     897..906
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              911..937
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       938..946
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      950..972
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      950..972
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 973..1042
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  977..999
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1000..1019
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1020..1042)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1043..1046
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1047..1069)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1047..1069)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 1047..1078
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1070..1094
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1079..1112
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1104..1238
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1113..1268
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 1269..3979
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(1510..1544)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(1556..1571)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(1780..1799)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      1901..1923
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(1962..1980)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2048..2152
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2153..3013
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2371..2390)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       3184..3772
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      3673..3692
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      3926..3943
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 3980..4068
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4069..6937
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4149..4170
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4185..4215
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     4223..4239
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      4247..4263
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    4289..4351
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     4527..4608
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      4635..4654
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(4725..5324)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      5210..5229
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(5325..5381)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(5391..6134)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(5532..5551)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      5777..5796
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(6135..6158)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6158..6167)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6187..6398)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(6399..6778)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      6927..6946
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 6938..6970
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 6971..7003
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(6980..6998)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGTTGG CATGTCTGTG GATCCCCTAT CCAGCAAAGC
      421 TCTAAAGATC AAGCGAGAGC TGAGCGAGAA CACGCCGCAC CTGTCGGACG AGGCGCTGAT
      481 GGGGCTGTCG GTGCGCGAGC TGAACCGGCA TCTGCGCGGG CTCTCCGCCG AGGAGGTGAC
      541 ACGGCTCAAG CAGCGGCGCC GCACACTCAA AAACCGTGGC TACGCCGCCA GCTGCCGCGT
      601 GAAGCGCGTG TGCCAGAAGG AGGAGCTGCA GAAGCAGAAG TCGGAGCTGG AGCGCGAGGT
      661 GGACAAGCTG GCGCGCGAGA ACGCCGCCAT GCGCCTGGAG CTCGACGCGC TGCGCGGCAA
      721 GTGCGAGGCG CTGCAGGGCT TCGCGCGCTC CGTGGCCGCC GCCCGCGGGC CCGCCACGCT
      781 CGTGGCGCCG GCCAGCGTCA TCACCATCGT CAAGTCCACC CCGGGCTCGG GGTCTGGCCC
      841 CGCCCACGGC CCGGACCCCG CCCACGGCCC GGCCTCCTGC TCCTACCCAG CTTTCTTGTA
      901 CAAAGTGGTA TACCCATACG ATGTTCCAGA TTACGCTTAG TAATGAGAAA TACTCAGAAG
      961 ATGTCACTCA CCATAGTCCG CAGTGAAAGA TAAATGATCN NNNNNNNNNN NNNNNNNNNG
     1021 TTTTAGAGCT AGAAATAGCA AGTAGGCCAC TGAGCAAGAA CTATGAATGA GATCTTTAGA
     1081 CATATTGCGG CCGCTTTCTC CGCGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
     1141 AAACCAAAAC TAGAATGCAG TGAAAAAAAT GCCTTATTTG TGAAATTTGT GATGCTATTG
     1201 CCTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAT CTATAACAAG AAAATATATA
     1261 TATAATAAGT TATCACGTAA GTAGAACATG AAATAACAAT ATAATTATCG TATGAGTTAA
     1321 ATCTTAAAAG TCACGTAAAA GATAATCATG CGTCATTTTG ACTCACGCGG TCGTTATAGT
     1381 TCAAAATCAG TGACACTTAC CGCATTGACA AGCACGCCTC ACGGGAGCTC CAAGCGGCGA
     1441 CTGAGATGTC CTAAATGCAC AGCGACGGAT TCGCGCTATT TAGAAAGAGA GAGCAATATT
     1501 TCAAGAATGC ATGCGTCAAT TTTACGCAGA CTATCTTTCT AGGGTTAAGA ATTCACTGGC
     1561 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
     1621 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
     1681 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA
     1741 TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC
     1801 ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT
     1861 GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCATGTCT CCGGGAGCTG CATGTGTCAG
     1921 AGGTTTTCAC CGTCATCACC GAAACACGCG AGTTCGAAAG GGCCTCGTGA TACGCCTATT
     1981 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
     2041 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     2101 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     2161 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     2221 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     2281 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     2341 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     2401 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     2461 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     2521 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     2581 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     2641 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     2701 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     2761 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     2821 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGTT CACGCGGTAT
     2881 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     2941 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     3001 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     3061 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     3121 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     3181 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
     3241 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
     3301 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
     3361 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
     3421 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
     3481 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
     3541 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
     3601 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
     3661 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
     3721 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
     3781 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
     3841 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
     3901 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG ATGAGCGCCC
     3961 AATACGCAAA CCGCCTCTCA CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
     4021 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAAAC CGCCTCTCCC CGCGCGTTGG
     4081 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
     4141 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
     4201 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
     4261 GACCATGATT ACGCCAAGGT CGACTTAACC CTAGAAAGAT AATCATATTG TGACGTACGT
     4321 TAAAGATAAT CATGCGTAAA ATTGACGCAT GTGTTTTATC GGTCTGTATA TCGAGGTTTA
     4381 TTTATTAATT TGAATAGATA TTAAGTTTTA TTATATTTAC ACTTACATAC TAATAATAAA
     4441 TTCAACAAAC AATTTATTTA TGTTTATTTA TTTATTAAAA AAAAACAAAA ACTCAAAATT
     4501 TCTTCTATAA AGTAACAAAA CTTTTAGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     4561 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     4621 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     4681 AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTGG AGATTCATGC GCCAGGTTTT
     4741 CTGGTCATGC ACCACGTTCG TGGGCCCTCA GGAACCTCCA CGTCAGCGGT AACTGTGAAG
     4801 CCCAGTCGCT CGTAAAAAGG AAGATTCCGG GGAGCACTGG TTTCGAGGAA CGCCGGCACG
     4861 CCAGCCCGTT CCGCGGCCTC AACACCAGGA AGAACGACAG CGCTTCCCAA ACCTTTACCT
     4921 TGGTGATCTG GGCTGACACC GACAGTGGCA AGAAACCACG CAGGCTCCTT TGGTCTATGA
     4981 GGAGCCAGCA AGCCCTCCAT TTGCTGTTGT GCCGCCAACC TACTCCCTGA CAGCTCGGCC
     5041 ATCCGCGGAC CTATTTCCGC GAACACTGCG CCGGCTTCGA CGCTTTCCGG AGTAGTCCAG
     5101 ACCGCAACTG CGGCACCATC GTCCGCAACC CAAACTTTAC CAATGTCCAG ACCCACGCGC
     5161 GTAAGAAACA GCTCTTGCAA CTCAGTTACC CTCTCTATGT GTCTGTCCGG ATCTACGGTA
     5221 TGGCGGGTCG CTGGATAATC TGCAAATGCT GCGGCAAGTG TTCGGACAGC CCGTGGTACA
     5281 TCGTCACGGG TAGCGAGCCT AACAGTGGGC TTATATTCCG TCATGGGGCC GGGGTTCTCC
     5341 TCCACGTCGC CGGCCTGCTT CAGCAGGCTG AAGTTGGTGG CGCCGCTGCC CCCGGGGAGC
     5401 ATGTCAAGGT CAAAATCGTC AAGAGCGTCA GCAGGCAGCA TATCAAGGTC AAAGTCGTCA
     5461 AGGGCATCGG CTGGGAGCAT GTCTAAGTCA AAATCGTCAA GGGCGTCGGT CGGCCCGCCG
     5521 CTTTCGCACT TTAGCTGTTT CTCCAGGCCA CATATGATTA GTTCCAGGCC GAAAAGGAAG
     5581 GCAGGTTCGG CTCCCTGCCG GTCGAACAGC TCAATTGCTT GTTTCAGAAG TGGGGGCATA
     5641 GAATCGGTGG TAGGTGTCTC TCTTTCCTCT TTTGCTACTT GATGCTCCTG TTCCTCCAAT
     5701 ACGCAGCCCA GTGTAAAGTG GCCCACGGCG GACAGAGCGT ACAGTGCGTT CTCCAGGGAG
     5761 AAGCCTTGCT GACACAGGAA CGCGAGCTGA TTTTCCAGGG TTTCGTACTG TTTCTCTGTT
     5821 GGGCGGGTGC CGAGATGCAC TTTAGCCCCG TCACGATGTG AGAGGAGAGC ACAGCGGTAT
     5881 GACTTGGCGT TGTTCCGCAG AAAGTCTTGC CATGACTCGC CTTCCAGGGG GCAGGAGTGG
     5941 GTATGATGCC TGTCCAGCAT CTCGATTGGC AGGGCATCGA GCAGGGCCCG CTTGTTCTTC
     6001 ACGTGCCAGT ACAGGGTAGG CTGCTCAACT CCCAGCTTTT GAGCGAGTTT CCTTGTCGTC
     6061 AGGCCTTCGA TACCGACTCC ATTGAGTAAT TCCAGAGCAG AGTTTATGAC TTTGCTCTTG
     6121 TCCAGTCTAG ACATCTTATC GTCATCGTCT TTGTAATCCA TGGTGGCGGA TCCCGCGTCA
     6181 CGACACCTGT GTTCTGGCGG CAAACCCGTT GCGAAAAAGA ACGTTCACGG CGACTACTGC
     6241 ACTTATATAC GGTTCTCCCC CACCCTCGGG AAAAAGGCGG AGCCAGTACA CGACATCACT
     6301 TTCCCAGTTT ACCCCGCGCC ACCTTCTCTA GGCACCGGTT CAATTGCCGA CCCCTCCCCC
     6361 CAACTTCTCG GGCACTGTGG GCCATGTGCG CTCTGCCCCA TGGTAATAGC CATGACTAAT
     6421 ACGTAGATGT ACTGCCAAGT AGGAAAGTGC CATAAGGTCA TGTACTGGGC ATAATGCCAG
     6481 GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA
     6541 TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTG
     6601 CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG
     6661 GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG
     6721 CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAGTCAATAA TCAATGTCAT
     6781 AAGCTCTAAT CGCATAATCG AGCTAAGAGC GATTAATATG CTTAGCTAGC TTAGCTAAGC
     6841 TAGATTTCTC TATTCTCCCA CAATATATAG CAGCTATTAG CTTAGCAGTC CGAATACGAT
     6901 CGTCCACGAT CCATAAGCGT AACACATTTG AGTAGCGACT GACGGGCACC GGAGCCACTC
     6961 GAGTGGAATT ACTGACGGGC ACCGGAGCCA CTCGAGTGGA ATT
//