LOCUS       CK530_25_ELK1           7795 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     387..391
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature     392..1677
                      /label="ELK1"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     CDS              392..1738
                      /label="Translation 392-1738"
     misc_feature     complement(1680..1698)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1684..1700)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1684..1701)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1684..1764
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1689..1698
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1703..1729
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       1730..1738
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      1742..1764
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      1742..1764
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 1765..1834
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1769..1791
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1792..1811
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1812..1834)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 1835..1838
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1839..1861)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(1839..1861)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 1839..1870
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            1862..1886
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 1871..1904
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     1896..2030
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 1905..2060
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2061..4771
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2302..2336)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2348..2363)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2572..2591)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2693..2715
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2754..2772)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         2840..2944
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2945..3805
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3163..3182)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       3976..4564
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4465..4484
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4718..4735
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4772..4860
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 4861..7729
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     4941..4962
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         4977..5007
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     5015..5031
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      5039..5055
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5081..5143
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5319..5400
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5427..5446
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5517..6116)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      6002..6021
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6117..6173)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6183..6926)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6324..6343)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6569..6588
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(6927..6950)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(6950..6959)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(6979..7190)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7191..7570)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7719..7738
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7730..7762
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7763..7795
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7772..7790)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGTTGG CATGGACCCA TCTGTGACGC TGTGGCAGTT
      421 TCTGCTGCAG CTGCTGAGAG AGCAAGGCAA TGGCCACATC ATCTCCTGGA CTTCACGGGA
      481 TGGTGGTGAA TTCAAGCTGG TGGATGCAGA GGAGGTGGCC CGGCTGTGGG GGCTACGCAA
      541 GAACAAGACC AACATGAATT ACGACAAGCT CAGCCGGGCC TTGCGGTACT ACTATGACAA
      601 GAACATCATC CGCAAGGTGA GCGGCCAGAA GTTCGTCTAC AAGTTTGTGT CCTACCCTGA
      661 GGTCGCAGGG TGCTCCACTG AGGACTGCCC GCCCCAGCCA GAGGTGTCTG TTACCTCCAC
      721 CATGCCAAAT GTGGCCCCTG CTGCTATACA TGCCGCCCCA GGGGACACTG TCTCTGGAAA
      781 GCCAGGCACA CCCAAGGGTG CAGGAATGGC AGGCCCAGGC GGTTTGGCAC GCAGCAGCCG
      841 GAACGAGTAC ATGCGCTCGG GCCTCTATTC CACCTTCACC ATCCAGTCTC TGCAGCCGCA
      901 GCCACCCCCT CATCCTCGGC CTGCTGTGGT GCTCCCCAGT GCAGCTCCTG CAGGGGCAGC
      961 AGCGCCCCCC TCGGGGAGCA GGAGCACCAG TCCAAGCCCC TTGGAGGCCT GTCTGGAGGC
     1021 TGAAGAGGCC GGCTTGCCTC TGCAGGTCAT CCTGACCCCG CCCGAGGCCC CAAACCTGAA
     1081 ATCGGAAGAG CTTAATGTGG AGCCGGGTTT GGGCCGGGCT TTGCCCCCAG AAGTGAAAGT
     1141 AGAAGGGCCC AAGGAAGAGT TGGAAGTTGC GGGGGAGAGA GGGTTTGTGC CAGAAACCAC
     1201 CAAGGCCGAG CCAGAAGTCC CTCCACAGGA GGGCGTGCCA GCCCGGCTGC CCGCGGTTGT
     1261 TATGGACACC GCAGGGCAGG CGGGCGGCCA TGCGGCTTCC AGCCCTGAGA TCTCCCAGCC
     1321 GCAGAAGGGC CGGAAGCCCC GGGACCTAGA GCTTCCACTC AGCCCGAGCC TGCTAGGTGG
     1381 GCCGGGACCC GAACGGACCC CAGGATCGGG AAGTGGCTCC GGCCTCCAGG CTCCGGGGCC
     1441 GGCGCTGACC CCATCCCTGC TTCCTACGCA TACATTGACC CCGGTGCTGC TGACACCCAG
     1501 CTCGCTGCCT CCTAGCATTC ACTTCTGGAG CACCCTGAGT CCCATTGCGC CCCGTAGCCC
     1561 GGCCAAGCTC TCCTTCCAGT TTCCATCCAG TGGCAGCGCC CAGGTGCACA TCCCTTCTAT
     1621 CAGCGTGGAT GGCCTCTCGA CCCCCGTGGT GCTCTCCCCA GGGCCCCAGA AGCCATGCCC
     1681 AGCTTTCTTG TACAAAGTGG TATACCCATA CGATGTTCCA GATTACGCTT AGTAATGAGA
     1741 AATACTCAGA AGATGTCACT CACCATAGTC CGCAGTGAAA GATAAATGAT CNNNNNNNNN
     1801 NNNNNNNNNN NGTTTTAGAG CTAGAAATAG CAAGTAGGCC ACTGAGCAAG AACTATGAAT
     1861 GAGATCTTTA GACATATTGC GGCCGCTTTC TCCGCGATCC AGACATGATA AGATACATTG
     1921 ATGAGTTTGG ACAAACCAAA ACTAGAATGC AGTGAAAAAA ATGCCTTATT TGTGAAATTT
     1981 GTGATGCTAT TGCCTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT ATCTATAACA
     2041 AGAAAATATA TATATAATAA GTTATCACGT AAGTAGAACA TGAAATAACA ATATAATTAT
     2101 CGTATGAGTT AAATCTTAAA AGTCACGTAA AAGATAATCA TGCGTCATTT TGACTCACGC
     2161 GGTCGTTATA GTTCAAAATC AGTGACACTT ACCGCATTGA CAAGCACGCC TCACGGGAGC
     2221 TCCAAGCGGC GACTGAGATG TCCTAAATGC ACAGCGACGG ATTCGCGCTA TTTAGAAAGA
     2281 GAGAGCAATA TTTCAAGAAT GCATGCGTCA ATTTTACGCA GACTATCTTT CTAGGGTTAA
     2341 GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
     2401 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
     2461 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT
     2521 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG
     2581 CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG
     2641 ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCATGT CTCCGGGAGC
     2701 TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACACG CGAGTTCGAA AGGGCCTCGT
     2761 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG
     2821 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
     2881 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
     2941 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
     3001 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
     3061 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
     3121 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
     3181 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
     3241 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
     3301 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
     3361 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
     3421 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
     3481 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
     3541 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
     3601 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
     3661 TTCACGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
     3721 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
     3781 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
     3841 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
     3901 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA
     3961 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA
     4021 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC
     4081 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA
     4141 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
     4201 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG
     4261 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG
     4321 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC
     4381 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG
     4441 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT
     4501 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG
     4561 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA
     4621 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG
     4681 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
     4741 GGATGAGCGC CCAATACGCA AACCGCCTCT CACCGCGCGT TGGCCGATTC ATTAATGCAG
     4801 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA ACCGCCTCTC
     4861 CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG
     4921 GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA
     4981 CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA
     5041 GGAAACAGCT ATGACCATGA TTACGCCAAG GTCGACTTAA CCCTAGAAAG ATAATCATAT
     5101 TGTGACGTAC GTTAAAGATA ATCATGCGTA AAATTGACGC ATGTGTTTTA TCGGTCTGTA
     5161 TATCGAGGTT TATTTATTAA TTTGAATAGA TATTAAGTTT TATTATATTT ACACTTACAT
     5221 ACTAATAATA AATTCAACAA ACAATTTATT TATGTTTATT TATTTATTAA AAAAAAACAA
     5281 AAACTCAAAA TTTCTTCTAT AAAGTAACAA AACTTTTAGC AGTGAAAAAA ATGCTTTATT
     5341 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT
     5401 AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT
     5461 TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCTCT GGAGATTCAT
     5521 GCGCCAGGTT TTCTGGTCAT GCACCACGTT CGTGGGCCCT CAGGAACCTC CACGTCAGCG
     5581 GTAACTGTGA AGCCCAGTCG CTCGTAAAAA GGAAGATTCC GGGGAGCACT GGTTTCGAGG
     5641 AACGCCGGCA CGCCAGCCCG TTCCGCGGCC TCAACACCAG GAAGAACGAC AGCGCTTCCC
     5701 AAACCTTTAC CTTGGTGATC TGGGCTGACA CCGACAGTGG CAAGAAACCA CGCAGGCTCC
     5761 TTTGGTCTAT GAGGAGCCAG CAAGCCCTCC ATTTGCTGTT GTGCCGCCAA CCTACTCCCT
     5821 GACAGCTCGG CCATCCGCGG ACCTATTTCC GCGAACACTG CGCCGGCTTC GACGCTTTCC
     5881 GGAGTAGTCC AGACCGCAAC TGCGGCACCA TCGTCCGCAA CCCAAACTTT ACCAATGTCC
     5941 AGACCCACGC GCGTAAGAAA CAGCTCTTGC AACTCAGTTA CCCTCTCTAT GTGTCTGTCC
     6001 GGATCTACGG TATGGCGGGT CGCTGGATAA TCTGCAAATG CTGCGGCAAG TGTTCGGACA
     6061 GCCCGTGGTA CATCGTCACG GGTAGCGAGC CTAACAGTGG GCTTATATTC CGTCATGGGG
     6121 CCGGGGTTCT CCTCCACGTC GCCGGCCTGC TTCAGCAGGC TGAAGTTGGT GGCGCCGCTG
     6181 CCCCCGGGGA GCATGTCAAG GTCAAAATCG TCAAGAGCGT CAGCAGGCAG CATATCAAGG
     6241 TCAAAGTCGT CAAGGGCATC GGCTGGGAGC ATGTCTAAGT CAAAATCGTC AAGGGCGTCG
     6301 GTCGGCCCGC CGCTTTCGCA CTTTAGCTGT TTCTCCAGGC CACATATGAT TAGTTCCAGG
     6361 CCGAAAAGGA AGGCAGGTTC GGCTCCCTGC CGGTCGAACA GCTCAATTGC TTGTTTCAGA
     6421 AGTGGGGGCA TAGAATCGGT GGTAGGTGTC TCTCTTTCCT CTTTTGCTAC TTGATGCTCC
     6481 TGTTCCTCCA ATACGCAGCC CAGTGTAAAG TGGCCCACGG CGGACAGAGC GTACAGTGCG
     6541 TTCTCCAGGG AGAAGCCTTG CTGACACAGG AACGCGAGCT GATTTTCCAG GGTTTCGTAC
     6601 TGTTTCTCTG TTGGGCGGGT GCCGAGATGC ACTTTAGCCC CGTCACGATG TGAGAGGAGA
     6661 GCACAGCGGT ATGACTTGGC GTTGTTCCGC AGAAAGTCTT GCCATGACTC GCCTTCCAGG
     6721 GGGCAGGAGT GGGTATGATG CCTGTCCAGC ATCTCGATTG GCAGGGCATC GAGCAGGGCC
     6781 CGCTTGTTCT TCACGTGCCA GTACAGGGTA GGCTGCTCAA CTCCCAGCTT TTGAGCGAGT
     6841 TTCCTTGTCG TCAGGCCTTC GATACCGACT CCATTGAGTA ATTCCAGAGC AGAGTTTATG
     6901 ACTTTGCTCT TGTCCAGTCT AGACATCTTA TCGTCATCGT CTTTGTAATC CATGGTGGCG
     6961 GATCCCGCGT CACGACACCT GTGTTCTGGC GGCAAACCCG TTGCGAAAAA GAACGTTCAC
     7021 GGCGACTACT GCACTTATAT ACGGTTCTCC CCCACCCTCG GGAAAAAGGC GGAGCCAGTA
     7081 CACGACATCA CTTTCCCAGT TTACCCCGCG CCACCTTCTC TAGGCACCGG TTCAATTGCC
     7141 GACCCCTCCC CCCAACTTCT CGGGCACTGT GGGCCATGTG CGCTCTGCCC CATGGTAATA
     7201 GCCATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT GCCATAAGGT CATGTACTGG
     7261 GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA TAGGGGGCGT ACTTGGCATA
     7321 TGATACACTT GATGTACTGC CAAGTGGGCA GTTTACCGTA AATACTCCAC CCATTGACGT
     7381 CAATGGAAAG TGCCTATTGG CGTTACTATG GGAACATACG TCATTATTGA CGTCAATGGG
     7441 CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG TAAGTTATGT AACGCGGAAC
     7501 TCCATATATG GGCTATGAAC TAATGACCCC GTAATTGATT ACTATTAATA ACTAGTCAAT
     7561 AATCAATGTC ATAAGCTCTA ATCGCATAAT CGAGCTAAGA GCGATTAATA TGCTTAGCTA
     7621 GCTTAGCTAA GCTAGATTTC TCTATTCTCC CACAATATAT AGCAGCTATT AGCTTAGCAG
     7681 TCCGAATACG ATCGTCCACG ATCCATAAGC GTAACACATT TGAGTAGCGA CTGACGGGCA
     7741 CCGGAGCCAC TCGAGTGGAA TTACTGACGG GCACCGGAGC CACTCGAGTG GAATT
//