LOCUS       CK530_32_NR4A1          8305 bp ds-DNA     circular     07-APR-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     392..2187
                      /label="NR4A1"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     CDS              392..2248
                      /label="Translation 392-2248"
     misc_feature     complement(2190..2208)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(2194..2210)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(2194..2211)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 2194..2274
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     2199..2208
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              2213..2239
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     terminator       2240..2248
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      2252..2274
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      2252..2274
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 2275..2344
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  2279..2301
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     2302..2321
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(2322..2344)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 2345..2348
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(2349..2371)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2349..2371)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 2349..2380
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            2372..2396
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 2381..2414
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     2406..2540
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 2415..2570
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2571..5281
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2812..2846)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2858..2873)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(3082..3101)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      3203..3225
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(3264..3282)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         3350..3454
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              3455..4315
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3673..3692)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       4486..5074
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4975..4994
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      5228..5245
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 5282..5370
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 5371..8239
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     5451..5472
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         5487..5517
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     5525..5541
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      5549..5565
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5591..5653
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5829..5910
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5937..5956
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(6027..6626)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      6512..6531
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6627..6683)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6693..7436)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6834..6853)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      7079..7098
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(7437..7460)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(7460..7469)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(7489..7700)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7701..8080)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      8229..8248
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 8240..8272
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 8273..8305
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(8282..8300)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGTTGG CATGCCCTGT ATCCAAGCCC AATATGGGAC
      421 ACCAGCACCG AGTCCGGGAC CCCGTGACCA CCTGGCAAGC GACCCCCTGA CCCCTGAGTT
      481 CATCAAGCCC ACCATGGACC TGGCCAGCCC CGAGGCAGCC CCCGCTGCCC CCACTGCCCT
      541 GCCCAGCTTC AGCACCTTCA TGGACGGCTA CACAGGAGAG TTTGACACCT TCCTCTACCA
      601 GCTGCCAGGA ACAGTCCAGC CATGCTCCTC AGCCTCCTCC TCGGCCTCCT CCACATCCTC
      661 GTCCTCAGCC ACCTCCCCTG CCTCTGCCTC CTTCAAGTTC GAGGACTTCC AGGTGTACGG
      721 CTGCTACCCC GGCCCCCTGA GCGGCCCAGT GGATGAGGCC CTGTCCTCCA GTGGCTCTGA
      781 CTACTATGGC AGCCCCTGCT CGGCCCCGTC GCCCTCCACG CCCAGCTTCC AGCCGCCCCA
      841 GCTCTCTCCC TGGGATGGCT CCTTCGGCCA CTTCTCGCCC AGCCAGACTT ACGAAGGCCT
      901 GCGGGCATGG ACAGAGCAGC TGCCCAAAGC CTCTGGGCCC CCACAGCCTC CAGCCTTCTT
      961 TTCCTTCAGT CCTCCCACCG GCCCCAGCCC CAGCCTGGCC CAGAGCCCCC TGAAGTTGTT
     1021 CCCCTCACAG GCCACCCACC AGCTGGGGGA GGGAGAGAGC TATTCCATGC CTACGGCCTT
     1081 CCCAGGTTTG GCACCCACTT CTCCACACCT TGAGGGCTCG GGGATACTGG ATACACCCGT
     1141 GACCTCAACC AAGGCCCGGA GCGGGGCCCC AGGTGGAAGT GAAGGCCGCT GTGCTGTGTG
     1201 TGGGGACAAC GCTTCATGCC AGCATTATGG TGTCCGCACA TGTGAGGGCT GCAAGGGCTT
     1261 CTTCAAGCGC ACAGTGCAGA AAAACGCCAA GTACATCTGC CTGGCTAACA AGGACTGCCC
     1321 TGTGGACAAG AGGCGGCGAA ACCGCTGCCA GTTCTGCCGC TTCCAGAAGT GCCTGGCGGT
     1381 GGGCATGGTG AAGGAAGTTG TCCGAACAGA CAGCCTGAAG GGGCGGCGGG GCCGGCTACC
     1441 TTCAAAACCC AAGCAGCCCC CAGATGCCTC CCCTGCCAAT CTCCTCACTT CCCTGGTCCG
     1501 TGCACACCTG GACTCAGGGC CCAGCACTGC CAAACTGGAC TACTCCAAGT TCCAGGAGCT
     1561 GGTGCTGCCC CACTTTGGGA AGGAAGATGC TGGGGATGTA CAGCAGTTCT ACGACCTGCT
     1621 CTCCGGTTCT CTGGAGGTCA TCCGCAAGTG GGCGGAGAAG ATCCCTGGCT TTGCTGAGCT
     1681 GTCACCGGCT GACCAGGACC TGTTGCTGGA GTCGGCCTTC CTGGAGCTCT TCATCCTCCG
     1741 CCTGGCGTAC AGGTCTAAGC CAGGCGAGGG CAAGCTCATC TTCTGCTCAG GCCTGGTGCT
     1801 ACACCGGCTG CAGTGTGCCC GTGGCTTCGG GGACTGGATT GACAGTATCC TGGCCTTCTC
     1861 AAGGTCCCTG CACAGCTTGC TTGTCGATGT CCCTGCCTTC GCCTGCCTCT CTGCCCTTGT
     1921 CCTCATCACC GACCGGCATG GGCTGCAGGA GCCGCGGCGG GTGGAGGAGC TGCAGAACCG
     1981 CATCGCCAGC TGCCTGAAGG AGCACGTGGC AGCTGTGGCG GGCGAGCCCC AGCCAGCCAG
     2041 CTGCCTGTCA CGTCTGTTGG GCAAACTGCC CGAGCTGCGG ACCCTGTGCA CCCAGGGCCT
     2101 GCAGCGCATC TTCTACCTCA AGCTGGAGGA CTTGGTGCCC CCTCCACCCA TCATTGACAA
     2161 GATCTTCATG GACACGCTGC CCTTCTGCCC AGCTTTCTTG TACAAAGTGG TATACCCATA
     2221 CGATGTTCCA GATTACGCTT AGTAATGAGA AATACTCAGA AGATGTCACT CACCATAGTC
     2281 CGCAGTGAAA GATAAATGAT CNNNNNNNNN NNNNNNNNNN NGTTTTAGAG CTAGAAATAG
     2341 CAAGTAGGCC ACTGAGCAAG AACTATGAAT GAGATCTTTA GACATATTGC GGCCGCTTTC
     2401 TCCGCGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCAAA ACTAGAATGC
     2461 AGTGAAAAAA ATGCCTTATT TGTGAAATTT GTGATGCTAT TGCCTTATTT GTAACCATTA
     2521 TAAGCTGCAA TAAACAAGTT ATCTATAACA AGAAAATATA TATATAATAA GTTATCACGT
     2581 AAGTAGAACA TGAAATAACA ATATAATTAT CGTATGAGTT AAATCTTAAA AGTCACGTAA
     2641 AAGATAATCA TGCGTCATTT TGACTCACGC GGTCGTTATA GTTCAAAATC AGTGACACTT
     2701 ACCGCATTGA CAAGCACGCC TCACGGGAGC TCCAAGCGGC GACTGAGATG TCCTAAATGC
     2761 ACAGCGACGG ATTCGCGCTA TTTAGAAAGA GAGAGCAATA TTTCAAGAAT GCATGCGTCA
     2821 ATTTTACGCA GACTATCTTT CTAGGGTTAA GAATTCACTG GCCGTCGTTT TACAACGTCG
     2881 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
     2941 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT
     3001 GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA
     3061 CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG
     3121 ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA
     3181 CAGACAAGCT GTGACCATGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
     3241 CCGAAACACG CGAGTTCGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT
     3301 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     3361 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     3421 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
     3481 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
     3541 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
     3601 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
     3661 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT
     3721 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
     3781 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
     3841 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
     3901 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
     3961 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG
     4021 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
     4081 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
     4141 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG TTCACGCGGT ATCATTGCAG CACTGGGGCC
     4201 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
     4261 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
     4321 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
     4381 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
     4441 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
     4501 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
     4561 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
     4621 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
     4681 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
     4741 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
     4801 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
     4861 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
     4921 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
     4981 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
     5041 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
     5101 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
     5161 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
     5221 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGATGAGCGC CCAATACGCA AACCGCCTCT
     5281 CACCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC
     5341 GGGCAGTGAG CGCAACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC
     5401 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT
     5461 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT
     5521 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG
     5581 GTCGACTTAA CCCTAGAAAG ATAATCATAT TGTGACGTAC GTTAAAGATA ATCATGCGTA
     5641 AAATTGACGC ATGTGTTTTA TCGGTCTGTA TATCGAGGTT TATTTATTAA TTTGAATAGA
     5701 TATTAAGTTT TATTATATTT ACACTTACAT ACTAATAATA AATTCAACAA ACAATTTATT
     5761 TATGTTTATT TATTTATTAA AAAAAAACAA AAACTCAAAA TTTCTTCTAT AAAGTAACAA
     5821 AACTTTTAGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
     5881 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
     5941 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
     6001 ATGGCTGATT ATGATCCTCT GGAGATTCAT GCGCCAGGTT TTCTGGTCAT GCACCACGTT
     6061 CGTGGGCCCT CAGGAACCTC CACGTCAGCG GTAACTGTGA AGCCCAGTCG CTCGTAAAAA
     6121 GGAAGATTCC GGGGAGCACT GGTTTCGAGG AACGCCGGCA CGCCAGCCCG TTCCGCGGCC
     6181 TCAACACCAG GAAGAACGAC AGCGCTTCCC AAACCTTTAC CTTGGTGATC TGGGCTGACA
     6241 CCGACAGTGG CAAGAAACCA CGCAGGCTCC TTTGGTCTAT GAGGAGCCAG CAAGCCCTCC
     6301 ATTTGCTGTT GTGCCGCCAA CCTACTCCCT GACAGCTCGG CCATCCGCGG ACCTATTTCC
     6361 GCGAACACTG CGCCGGCTTC GACGCTTTCC GGAGTAGTCC AGACCGCAAC TGCGGCACCA
     6421 TCGTCCGCAA CCCAAACTTT ACCAATGTCC AGACCCACGC GCGTAAGAAA CAGCTCTTGC
     6481 AACTCAGTTA CCCTCTCTAT GTGTCTGTCC GGATCTACGG TATGGCGGGT CGCTGGATAA
     6541 TCTGCAAATG CTGCGGCAAG TGTTCGGACA GCCCGTGGTA CATCGTCACG GGTAGCGAGC
     6601 CTAACAGTGG GCTTATATTC CGTCATGGGG CCGGGGTTCT CCTCCACGTC GCCGGCCTGC
     6661 TTCAGCAGGC TGAAGTTGGT GGCGCCGCTG CCCCCGGGGA GCATGTCAAG GTCAAAATCG
     6721 TCAAGAGCGT CAGCAGGCAG CATATCAAGG TCAAAGTCGT CAAGGGCATC GGCTGGGAGC
     6781 ATGTCTAAGT CAAAATCGTC AAGGGCGTCG GTCGGCCCGC CGCTTTCGCA CTTTAGCTGT
     6841 TTCTCCAGGC CACATATGAT TAGTTCCAGG CCGAAAAGGA AGGCAGGTTC GGCTCCCTGC
     6901 CGGTCGAACA GCTCAATTGC TTGTTTCAGA AGTGGGGGCA TAGAATCGGT GGTAGGTGTC
     6961 TCTCTTTCCT CTTTTGCTAC TTGATGCTCC TGTTCCTCCA ATACGCAGCC CAGTGTAAAG
     7021 TGGCCCACGG CGGACAGAGC GTACAGTGCG TTCTCCAGGG AGAAGCCTTG CTGACACAGG
     7081 AACGCGAGCT GATTTTCCAG GGTTTCGTAC TGTTTCTCTG TTGGGCGGGT GCCGAGATGC
     7141 ACTTTAGCCC CGTCACGATG TGAGAGGAGA GCACAGCGGT ATGACTTGGC GTTGTTCCGC
     7201 AGAAAGTCTT GCCATGACTC GCCTTCCAGG GGGCAGGAGT GGGTATGATG CCTGTCCAGC
     7261 ATCTCGATTG GCAGGGCATC GAGCAGGGCC CGCTTGTTCT TCACGTGCCA GTACAGGGTA
     7321 GGCTGCTCAA CTCCCAGCTT TTGAGCGAGT TTCCTTGTCG TCAGGCCTTC GATACCGACT
     7381 CCATTGAGTA ATTCCAGAGC AGAGTTTATG ACTTTGCTCT TGTCCAGTCT AGACATCTTA
     7441 TCGTCATCGT CTTTGTAATC CATGGTGGCG GATCCCGCGT CACGACACCT GTGTTCTGGC
     7501 GGCAAACCCG TTGCGAAAAA GAACGTTCAC GGCGACTACT GCACTTATAT ACGGTTCTCC
     7561 CCCACCCTCG GGAAAAAGGC GGAGCCAGTA CACGACATCA CTTTCCCAGT TTACCCCGCG
     7621 CCACCTTCTC TAGGCACCGG TTCAATTGCC GACCCCTCCC CCCAACTTCT CGGGCACTGT
     7681 GGGCCATGTG CGCTCTGCCC CATGGTAATA GCCATGACTA ATACGTAGAT GTACTGCCAA
     7741 GTAGGAAAGT GCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA
     7801 TTGACGTCAA TAGGGGGCGT ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA
     7861 GTTTACCGTA AATACTCCAC CCATTGACGT CAATGGAAAG TGCCTATTGG CGTTACTATG
     7921 GGAACATACG TCATTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG
     7981 CCATTTACCG TAAGTTATGT AACGCGGAAC TCCATATATG GGCTATGAAC TAATGACCCC
     8041 GTAATTGATT ACTATTAATA ACTAGTCAAT AATCAATGTC ATAAGCTCTA ATCGCATAAT
     8101 CGAGCTAAGA GCGATTAATA TGCTTAGCTA GCTTAGCTAA GCTAGATTTC TCTATTCTCC
     8161 CACAATATAT AGCAGCTATT AGCTTAGCAG TCCGAATACG ATCGTCCACG ATCCATAAGC
     8221 GTAACACATT TGAGTAGCGA CTGACGGGCA CCGGAGCCAC TCGAGTGGAA TTACTGACGG
     8281 GCACCGGAGC CACTCGAGTG GAATT
//