LOCUS       CK530_35_RUNX1          7960 bp ds-DNA     circular     01-JUL-2021
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted region 1..383
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     promoter         9..264
                      /label="TRE3GS Promoter"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     protein_bind     17..35
                      /label="tet operator (1)"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     53..71
                      /label="tet operator (2)"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     protein_bind     89..107
                      /label="tet operator (3)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     125..143
                      /label="tet operator (4)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind     161..179
                      /label="tet operator (5)"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     protein_bind     197..215
                      /label="tet operator (6)"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind     233..251
                      /label="tet operator (7)"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     promoter         265..364
                      /label="mCMV Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           322..342
                      /label="mCMV_seq"
                      /note="sequence: GCTGTTTTGACCTCCATAGAA"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer           348..367
                      /label="MPS324_mCMV_fwd2"
                      /note="sequence: CGGGACCGATCCAGCCTACC"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     protein_bind     368..383
                      /label="attR1"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     attR1            369..383
                      /label="attR1"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(373..376)
                      /label="attL1"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     misc_feature     373..392
                      /label="attB1_fragment"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature     387..398
                      /label="Kozak?"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     exon             399..456
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     CDS              399..1841
                      /label="RUNX1 CDS (runt-related transcription factor 1 isoform AML1c)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     CDS              399..1841
                      /label="Translation 399-1841"
     exon             457..495
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           complement(476..495)
                      /label="qMPS10R"
                      /note="sequence: cgtggacgtctctagaagga"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     exon             496..749
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     exon             750..906
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     exon             907..1011
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     exon             1012..1203
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     exon             1204..1365
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     primer           1355..1374
                      /label="RUNX1_3'"
                      /note="sequence: tcgactctcaacggcacccg"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     exon             1366..1841
                      /label="RUNX1 exon"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     misc_feature     1842..1863
                      /label="attB2_fragment"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     misc_feature     complement(1845..1863)
                      /label="attP"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     attR2            complement(1849..1865)
                      /label="attR2"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     protein_bind     complement(1849..1866)
                      /label="attR2"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     extracted region 1849..1929
                      /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1854..1863
                      /label="attL2"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              1868..1894
                      /label="HA Tag"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     primer           complement(1868..1894)
                      /label="MPS323_HA_rev"
                      /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     terminator       1895..1903
                      /label="STOPS"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer           1901..1929
                      /label="MPS311_BC_fwd2"
                      /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer           complement(1904..1929)
                      /label="MPS312-gBlock_attR2-DD_rev"
                      /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                      /ApEinfo_revcolor="#9eafd2"
                      /ApEinfo_fwdcolor="#9eafd2"
     primer_bind      1907..1929
                      /label="oAMP123-PCR1_NGS_BC_Fwd"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind      1907..1929
                      /label="BC_Fwd"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     extracted region 1930..1999
                      /label="Extracted region from Barcode Fragment"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     misc_difference  1934..1956
                      /label="Barcode linker 5'"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     misc_feature     1957..1976
                      /label="20mer Barcode"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference  complement(1977..1999)
                      /label="Barcode linker 3'"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 2000..2003
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind      complement(2004..2026)
                      /label="oAMP124-PCR1_NGS_BC_Rev"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(2004..2026)
                      /label="BC_Rev"
                      /ApEinfo_revcolor="#ff9ccd"
                      /ApEinfo_fwdcolor="#ff9ccd"
     extracted region 2004..2035
                      /label="Extracted region from Rev Primer"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     motif            2027..2051
                      /label="FP NotI_BgIII cloning"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     extracted region 2036..2069
                      /label="Extracted region from ATTR2-Right-FP modular"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal     2061..2195
                      /label="SV40 poly(A) signal"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     extracted region 2070..2225
                      /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     extracted region 2226..4936
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     repeat_region    complement(2467..2501)
                      /label="piggyBac left (5') inverted repeat"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     primer_bind      complement(2513..2528)
                      /label="M13F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      complement(2737..2756)
                      /label="pRS-marker"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      2858..2880
                      /label="pGEX 3'"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      complement(2919..2937)
                      /label="pBRforEco"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     promoter         3005..3109
                      /label="AmpR promoter"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     CDS              3110..3970
                      /label="AmpR"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      complement(3328..3347)
                      /label="Amp-R"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     rep_origin       4141..4729
                      /label="ori"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     primer_bind      4630..4649
                      /label="pBR322ori-F"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      4883..4900
                      /label="L4440"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     extracted region 4937..5025
                      /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                      /ApEinfo_revcolor="#b7e6d7"
                      /ApEinfo_fwdcolor="#b7e6d7"
     extracted region 5026..7894
                      /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind     5106..5127
                      /label="CAP binding site"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     promoter         5142..5172
                      /label="lac promoter"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind     5180..5196
                      /label="lac operator"
                      /ApEinfo_revcolor="#75c6a9"
                      /ApEinfo_fwdcolor="#75c6a9"
     primer_bind      5204..5220
                      /label="M13R"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     repeat_region    5246..5308
                      /label="piggyBac right (3') inverted repeat"
                      /ApEinfo_revcolor="#f58a5e"
                      /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal     5484..5565
                      /label="SV40 poly(A) signal(1)"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind      5592..5611
                      /label="Puro_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#c7b0e3"
                      /ApEinfo_fwdcolor="#c7b0e3"
     CDS              complement(5682..6281)
                      /label="Puromycin"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     primer_bind      6167..6186
                      /label="Tet-ON_Fwd"
                      /ApEinfo_revcolor="#84b0dc"
                      /ApEinfo_fwdcolor="#84b0dc"
     CDS              complement(6282..6338)
                      /label="P2A"
                      /ApEinfo_revcolor="#c6c9d1"
                      /ApEinfo_fwdcolor="#c6c9d1"
     CDS              complement(6348..7091)
                      /label="Tet-On (3G)"
                      /ApEinfo_revcolor="#d6b295"
                      /ApEinfo_fwdcolor="#d6b295"
     primer_bind      complement(6489..6508)
                      /label="Puro_Rev_Seq Primer"
                      /ApEinfo_revcolor="#b4abac"
                      /ApEinfo_fwdcolor="#b4abac"
     primer_bind      6734..6753
                      /label="CMV-Enhancer_Fwd_Seq Primer"
                      /ApEinfo_revcolor="#faac61"
                      /ApEinfo_fwdcolor="#faac61"
     CDS              complement(7092..7115)
                      /label="FLAG"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     regulatory       complement(7115..7124)
                      /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
     promoter         complement(7144..7355)
                      /label="EF-1-alpha core promoter"
                      /ApEinfo_revcolor="#d59687"
                      /ApEinfo_fwdcolor="#d59687"
     regulatory       complement(7356..7735)
                      /label="CMV Enhancer"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     primer_bind      7884..7903
                      /label="pTet_Fwd"
                      /ApEinfo_revcolor="#ffef86"
                      /ApEinfo_fwdcolor="#ffef86"
     extracted region 7895..7927
                      /label="Extracted region from Base Vector- PB-Tet-Puro"
                      /ApEinfo_revcolor="#b1ff67"
                      /ApEinfo_fwdcolor="#b1ff67"
     extracted region 7928..7960
                      /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                      /ApEinfo_revcolor="#f8d3a9"
                      /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind      complement(7937..7955)
                      /label="CMV-Enhancer_Rev_Seq Primer"
                      /ApEinfo_revcolor="#85dae9"
                      /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTGCCACCat ggcttcagac agcatatttg
      421 agtcatttcc ttcgtaccca cagtgcttca tgagagaatg catacttgga atgaatcctt
      481 ctagagacgt ccacgatgcc agcacgagcc gccgcttcac gccgccttcc accgcgctga
      541 gcccaggcaa gatgagcgag gcgttgccgc tgggcgcccc ggacgccggc gctgccctgg
      601 ccggcaagct gaggagcggc gaccgcagca tggtggaggt gctggccgac cacccgggcg
      661 agctggtgcg caccgacagc cccaacttcc tctgctccgt gctgcctacg cactggcgct
      721 gcaacaagac cctgcccatc gctttcaagg tggtggccct aggggatgtt ccagatggca
      781 ctctggtcac tgtgatggct ggcaatgatg aaaactactc ggctgagctg agaaatgcta
      841 ccgcagccat gaagaaccag gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg
      901 gaagagggaa aagcttcact ctgaccatca ctgtcttcac aaacccaccg caagtcgcca
      961 cctaccacag agccatcaaa atcacagtgg atgggccccg agaacctcga agacatcggc
     1021 agaaactaga tgatcagacc aagcccggga gcttgtcctt ttccgagcgg ctcagtgaac
     1081 tggagcagct gcggcgcaca gccatgaggg tcagcccaca ccacccagcc cccacgccca
     1141 accctcgtgc ctccctgaac cactccactg cctttaaccc tcagcctcag agtcagatgc
     1201 aggatacaag gcagatccaa ccatccccac cgtggtccta cgatcagtcc taccaatacc
     1261 tgggatccat tgcctctcct tctgtgcacc cagcaacgcc catttcacct ggacgtgcca
     1321 gcggcatgac aaccctctct gcagaacttt ccagtcgact ctcaacggca cccgacctga
     1381 cagcgttcag cgacccgcgc cagttccccg cgctgccctc catctccgac ccccgcatgc
     1441 actatccagg cgccttcacc tactccccga cgccggtcac ctcgggcatc ggcatcggca
     1501 tgtcggccat gggctcggcc acgcgctacc acacctacct gccgccgccc taccccggct
     1561 cgtcgcaagc gcagggaggc ccgttccaag ccagctcgcc ctcctaccac ctgtactacg
     1621 gcgcctcggc cggctcctac cagttctcca tggtgggcgg cgagcgctcg ccgccgcgca
     1681 tcctgccgcc ctgcaccaac gcctccaccg gctccgcgct gctcaacccc agcctcccga
     1741 accagagcga cgtggtggag gccgagggca gccacagcaa ctcccccacc aacatggcgc
     1801 cctccgcgcg cctggaggag gccgtgtgga ggccctactg aACCCAGCTT TCTTGTACAA
     1861 AGTGGTATAC CCATACGATG TTCCAGATTA CGCTTAGTAA TGAGAAATAC TCAGAAGATG
     1921 TCACTCACCA TAGTCCGCAG TGAAAGATAA ATGATCNNNN NNNNNNNNNN NNNNNNGTTT
     1981 TAGAGCTAGA AATAGCAAGT AGGCCACTGA GCAAGAACTA TGAATGAGAT CTTTAGACAT
     2041 ATTGCGGCCG CTTTCTCCGC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
     2101 CCAAAACTAG AATGCAGTGA AAAAAATGCC TTATTTGTGA AATTTGTGAT GCTATTGCCT
     2161 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTATCTA TAACAAGAAA ATATATATAT
     2221 AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC
     2281 TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA
     2341 AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG
     2401 AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA
     2461 AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAAGAATT CACTGGCCGT
     2521 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
     2581 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
     2641 ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT
     2701 GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
     2761 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT
     2821 CCCGGCATCC GCTTACAGAC AAGCTGTGAC CATGTCTCCG GGAGCTGCAT GTGTCAGAGG
     2881 TTTTCACCGT CATCACCGAA ACACGCGAGT TCGAAAGGGC CTCGTGATAC GCCTATTTTT
     2941 ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA
     3001 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     3061 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA
     3121 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA
     3181 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA
     3241 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT
     3301 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC
     3361 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC
     3421 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC
     3481 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA
     3541 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA
     3601 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT
     3661 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA
     3721 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC
     3781 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGTTCAC GCGGTATCAT
     3841 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG
     3901 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
     3961 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     4021 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     4081 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     4141 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     4201 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     4261 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     4321 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     4381 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     4441 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     4501 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
     4561 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     4621 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
     4681 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
     4741 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
     4801 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
     4861 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGATG AGCGCCCAAT
     4921 ACGCAAACCG CCTCTCACCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
     4981 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
     5041 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
     5101 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
     5161 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
     5221 CATGATTACG CCAAGGTCGA CTTAACCCTA GAAAGATAAT CATATTGTGA CGTACGTTAA
     5281 AGATAATCAT GCGTAAAATT GACGCATGTG TTTTATCGGT CTGTATATCG AGGTTTATTT
     5341 ATTAATTTGA ATAGATATTA AGTTTTATTA TATTTACACT TACATACTAA TAATAAATTC
     5401 AACAAACAAT TTATTTATGT TTATTTATTT ATTAAAAAAA AACAAAAACT CAAAATTTCT
     5461 TCTATAAAGT AACAAAACTT TTAGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT
     5521 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC
     5581 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC
     5641 CTCTACAAAT GTGGTATGGC TGATTATGAT CCTCTGGAGA TTCATGCGCC AGGTTTTCTG
     5701 GTCATGCACC ACGTTCGTGG GCCCTCAGGA ACCTCCACGT CAGCGGTAAC TGTGAAGCCC
     5761 AGTCGCTCGT AAAAAGGAAG ATTCCGGGGA GCACTGGTTT CGAGGAACGC CGGCACGCCA
     5821 GCCCGTTCCG CGGCCTCAAC ACCAGGAAGA ACGACAGCGC TTCCCAAACC TTTACCTTGG
     5881 TGATCTGGGC TGACACCGAC AGTGGCAAGA AACCACGCAG GCTCCTTTGG TCTATGAGGA
     5941 GCCAGCAAGC CCTCCATTTG CTGTTGTGCC GCCAACCTAC TCCCTGACAG CTCGGCCATC
     6001 CGCGGACCTA TTTCCGCGAA CACTGCGCCG GCTTCGACGC TTTCCGGAGT AGTCCAGACC
     6061 GCAACTGCGG CACCATCGTC CGCAACCCAA ACTTTACCAA TGTCCAGACC CACGCGCGTA
     6121 AGAAACAGCT CTTGCAACTC AGTTACCCTC TCTATGTGTC TGTCCGGATC TACGGTATGG
     6181 CGGGTCGCTG GATAATCTGC AAATGCTGCG GCAAGTGTTC GGACAGCCCG TGGTACATCG
     6241 TCACGGGTAG CGAGCCTAAC AGTGGGCTTA TATTCCGTCA TGGGGCCGGG GTTCTCCTCC
     6301 ACGTCGCCGG CCTGCTTCAG CAGGCTGAAG TTGGTGGCGC CGCTGCCCCC GGGGAGCATG
     6361 TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA GTCGTCAAGG
     6421 GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT
     6481 TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA AAGGAAGGCA
     6541 GGTTCGGCTC CCTGCCGGTC GAACAGCTCA ATTGCTTGTT TCAGAAGTGG GGGCATAGAA
     6601 TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGTTC CTCCAATACG
     6661 CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC CAGGGAGAAG
     6721 CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT CTCTGTTGGG
     6781 CGGGTGCCGA GATGCACTTT AGCCCCGTCA CGATGTGAGA GGAGAGCACA GCGGTATGAC
     6841 TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA GGAGTGGGTA
     6901 TGATGCCTGT CCAGCATCTC GATTGGCAGG GCATCGAGCA GGGCCCGCTT GTTCTTCACG
     6961 TGCCAGTACA GGGTAGGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG
     7021 CCTTCGATAC CGACTCCATT GAGTAATTCC AGAGCAGAGT TTATGACTTT GCTCTTGTCC
     7081 AGTCTAGACA TCTTATCGTC ATCGTCTTTG TAATCCATGG TGGCGGATCC CGCGTCACGA
     7141 CACCTGTGTT CTGGCGGCAA ACCCGTTGCG AAAAAGAACG TTCACGGCGA CTACTGCACT
     7201 TATATACGGT TCTCCCCCAC CCTCGGGAAA AAGGCGGAGC CAGTACACGA CATCACTTTC
     7261 CCAGTTTACC CCGCGCCACC TTCTCTAGGC ACCGGTTCAA TTGCCGACCC CTCCCCCCAA
     7321 CTTCTCGGGC ACTGTGGGCC ATGTGCGCTC TGCCCCATGG TAATAGCCAT GACTAATACG
     7381 TAGATGTACT GCCAAGTAGG AAAGTGCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG
     7441 GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT
     7501 ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTGCCT
     7561 ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC
     7621 GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA
     7681 TGAACTAATG ACCCCGTAAT TGATTACTAT TAATAACTAG TCAATAATCA ATGTCATAAG
     7741 CTCTAATCGC ATAATCGAGC TAAGAGCGAT TAATATGCTT AGCTAGCTTA GCTAAGCTAG
     7801 ATTTCTCTAT TCTCCCACAA TATATAGCAG CTATTAGCTT AGCAGTCCGA ATACGATCGT
     7861 CCACGATCCA TAAGCGTAAC ACATTTGAGT AGCGACTGAC GGGCACCGGA GCCACTCGAG
     7921 TGGAATTACT GACGGGCACC GGAGCCACTC GAGTGGAATT
//