LOCUS       pShuttle-PR.Cre         4918 bp ds-DNA     circular     05-OCT-2022
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            F152-shuttle-p53-Rb1-Crev2.gb ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      268..295
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnBgene"
                     /ApEinfo_revcolor="pink"
     terminator      387..473
                     /label="Escherichia coli rrnB"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="rrnB T1 terminator"
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnBgene"
                     /ApEinfo_revcolor="pink"
     primer_bind     537..553
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similarvariants"
                     /ApEinfo_revcolor="pink"
     protein_bind    569..668
                     /label="attL"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="attL1"
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /ApEinfo_revcolor="pink"
     misc_feature    680..920
                     /label="U6 Promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="U6 Promoter(1)"
                     /ApEinfo_revcolor="green"
     misc_feature    925..949
                     /label="sgTrp53"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#80ff00"
     misc_feature    950..1025
                     /label="gDNA scaffold"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="gDNA scaffold(1)"
                     /ApEinfo_revcolor="green"
     misc_feature    1032..1272
                     /label="U6 Promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="green"
     misc_feature    1277..1300
                     /label="sgRb1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    1301..1376
                     /label="gDNA scaffold"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="green"
     misc_feature    1386..1689
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1690..1893
                     /label="CMV Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1988..3028
                     /label="nls-Cre"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     protein_bind    complement(3043..3142)
                     /label="attL"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="attL2"
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="mutant version of attL(1)"
                     /ApEinfo_revcolor="pink"
     promoter        complement(3160..3178)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /ApEinfo_revcolor="#dddddd"
     primer_bind     complement(3183..3199)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similarvariants"
                     /ApEinfo_revcolor="pink"
     CDS             3312..4121
                     /label="KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="KanR"
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin in bacteria or G418(Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /ApEinfo_revcolor="#0000ff"
     rep_origin      4268..4856
                     /label="ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ori"
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"
                     /ApEinfo_revcolor="#ff0000"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcacc atggaGGCTG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG
      721 ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA AGATATTAGT
      781 ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG
      841 TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
      901 ATATATCTTG TGGAAAGGAC GAAACACCGC ATAAGGTACC ACCACGCTGG TTTTAGAGCT
      961 AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC
     1021 GGTGCTTTTT TGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG
     1081 CTGTTAGAGA GATAATTGGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA
     1141 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA
     1201 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT
     1261 TGTGGAAAGG ACGAAACACC GAACAGATTT GTCCTTCCCG GTTTTAGAGC TAGAAATAGC
     1321 AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT
     1381 TTccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1441 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1501 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1561 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1621 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1681 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
     1741 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1801 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1861 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1921 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag gatctaccat
     1981 gcccaagaag aagaggaagg tgtccaattt actgaccgta caccaaaatt tgcctgcatt
     2041 accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg
     2101 ccaggcgttt tctgagcata cctggaaaat gcttcagtcc gtttgccggt cgtgggcggc
     2161 atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta
     2221 tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca
     2281 gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc
     2341 actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc
     2401 tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg
     2461 ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat
     2521 agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt
     2581 aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag
     2641 cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc
     2701 gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag
     2761 ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg
     2821 cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg
     2881 agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg
     2941 ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc
     3001 aatggtgcgc ctgctggaag atggcgatTA GGGAATTCTA GAacccagct ttcttgtaca
     3061 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3121 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3181 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3241 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     3301 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     3361 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     3421 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     3481 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     3541 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     3601 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     3661 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     3721 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     3781 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     3841 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3901 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3961 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     4021 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     4081 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     4141 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     4201 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4621 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     4681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//