LOCUS       pSJ304_-_pLenti:_mCMV_ 12408 bp ds-DNA     circular     06-OCT-2022
DEFINITION  .
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(174..833)
                     /label="AmpR"
     misc_feature    1418..1981
                     /label="CMV PROMOTER"
     misc_feature    1999..2179
                     /label="HIV LTR 1"
     misc_feature    3612..3627
                     /label="cPPT"
     misc_feature    3641..3662
                     /label="pSicoR shRNA sequencing oligo"
     misc_recomb     3796..3815
                     /label="AttR1"
     promoter        3826..4361
                     /label="mCMV promoter"
     primer          complement(4343..4374)
                     /label="t803"
                     /note="sequence: catggtggAtcACtctgcgttctacggtggtc"
     primer          complement(4348..4371)
                     /label="oSJ353"
                     /note="sequence: gtccccggtggAtcACtctgcgttctacgg"
     primer          complement(4349..4381)
                     /label="oSJ328"
                     /note="sequence: gctctggcatggtggAtcACtctgcgttctacg"
     primer          4372..4388
                     /label="oSJ354"
                     /note="sequence: agaGTgaTccaccggggacgcgaggactgt"
     misc_feature    4372..4636
                     /label="5' UTR"
     primer          4540..4559
                     /label="oSJ103"
                     /note="sequence: ttgtctctttctccgcgcgc"
     primer          complement(4623..4640)
                     /label="oSJ355"
                     /note="sequence: cgccggcgccctccatggcgaatcggtgcc"
     primer          4627..4647
                     /label="t747"
                     /note="sequence: gtgaTCCACCatggagggcgc"
     primer          4637..4652
                     /label="oSJ215"
                     /note="sequence: atggagggcgccggcg"
     gene            4637..7870
                     /label="Hif1a gene"
     CDS             4637..9229
                     /label="Translation 4637-9229"
     primer          complement(4719..4737)
                     /label="t365"
                     /note="sequence: gactctttgcttcgccgag"
     primer          5409..5428
                     /label="oSJ104"
                     /note="sequence: gaattactgagttgatgggt"
     primer          complement(5571..5602)
                     /label="t636"
                     /note="sequence: agttgcTTCagtttcaacccagacatatccac"
     misc_feature    5594..5596
                     /label="Q320E"
     misc_feature    5642..5644
                     /label="V336E"
     misc_feature    5654..5656
                     /label="Y340T"
     primer          5824..5844
                     /label="oSJ221"
                     /note="sequence: tctcactctgctggctGCAgc"
     primer          complement(5824..5844)
                     /label="oSJ223"
                     /note="sequence: gcTGCagccagcagagtgaga"
     primer          5875..5893
                     /label="t727"
                     /note="sequence: tggcagcgatgacacagaa"
     misc_feature    5894..5902
                     /label="Linker"
     misc_feature    5903..6610
                     /label="mNeonGreen"
     primer          6409..6428
                     /label="oSJ364"
                     /note="sequence: CACCACTGGAAATGGCAAGC"
     primer          complement(6589..6610)
                     /label="oSJ351"
                     /note="sequence: ttcagtTCCTGAGCCCTTGTACAGCTCGTCCATGCCC"
     primer          6610..6637
                     /label="oSJ352"
                     /note="sequence: GCTGTACAAGGGCTCAGGAactgaagatcaacaactt"
     misc_feature    6611..6619
                     /label="Linker"
     primer          complement(6919..6941)
                     /label="oSJ359"
                     /note="sequence: TAGGCTGGGAAAAGTTAGGAGTG"
     misc_feature    7748..7870
                     /label="CTAD"
     misc_feature    7775..7777
                     /label="L805A"
     primer          complement(7841..7870)
                     /label="oSJ331"
                     /note="sequence: cgttaggggggggggTTAgttaacttgatccaaagctctgagtaattc"
     primer          7865..7884
                     /label="oSJ418"
                     /note="sequence: ctgctggggcaggttaacGGTGGCGGTGGCAG"
     primer          complement(7870..7885)
                     /label="oSJ395"
                     /note="sequence: TCCGCTTCCGCTGCCACCGCCACCg"
     misc_feature    7871..7885
                     /label="Linker"
     primer          7871..7886
                     /label="oSJ412"
                     /note="sequence: gctggggcagGGTGGCGGTGGCAGCG"
     primer          7886..7905
                     /label="oSJ35"
                     /note="sequence: GGAAGCGGAGCTACTAACTT"
     primer          7886..7909
                     /label="oSJ396"
                     /note="sequence: GGCGGTGGCAGCGGAAGCGGAGCTACTAACTTCAGC"
     primer          7886..7910
                     /label="oSJ54"
                     /note="sequence: GGAAGCGGAGCTACTAACTTCAGCC"
     misc_feature    7886..7951
                     /label="P2A"
     primer          7895..7922
                     /label="t410"
                     /note="sequence: GCTACTAACTTCAGCCTGCTGAAGCAGG"
     primer          7952..7971
                     /label="oSJ333"
                     /note="sequence: gataatatggccacaatgccagagccagcgaagtc"
     primer          7952..7973
                     /label="oSJ195"
                     /note="sequence: atgccagagccagcgaagtctg"
     Protein         7952..8329
                     /label="H2B"
     Linker          8330..8383
                     /label="Linker"
     misc_feature    8384..9100
                     /label="mCerulean"
     primer          complement(8451..8473)
                     /label="oSJ113"
                     /note="sequence: cacgctgaacttgtggccgttta"
     primer          complement(8522..8536)
                     /label="oSJ440"
                     /note="sequence: tgggccagggcacgggcagcttgccggtggtgcagatgaa"
     primer          8989..9013
                     /label="oSJ441"
                     /note="sequence: cgacaaccactacctgagcacccagtccaagctgagcaaa"
     primer          complement(9079..9100)
                     /label="oSJ338"
                     /note="sequence: CTCGAGTGCGGCCGCttActtgtacagctcgtccatgccg"
     primer          9101..9120
                     /label="oSJ375"
                     /note="sequence: ggatggacgagctgtacaagAAGCTTAGCCATGGCTTCCC"
     misc_feature    9101..9226
                     /label="PEST (d2 from mouse odc1)"
     primer          complement(9204..9226)
                     /label="oSJ377"
                     /note="sequence: TCGAGTGCGGCCGCttACACATTGATCCTAGCAGAAGCAC"
     primer          9227..9244
                     /label="oSJ378"
                     /note="sequence: GCTAGGATCAATGTGTaaGCGGCCGCACTCGAG"
     misc_recomb     complement(9256..9277)
                     /label="AttR2"
     misc_feature    9307..9882
                     /label="WPRE"
     primer          9823..9840
                     /label="oSJ200"
                     /note="sequence: tctgcggcctcttccgcg"
     promoter        10035..10548
                     /label="pgk"
     primer          10477..10497
                     /label="KL354"
                     /note="sequence: CATTCTGCACGCTTCAAAAGC"
     misc_feature    10588..11187
                     /label="puro resistance gene"
     misc_feature    11241..11256
                     /label="cPPT"
     misc_feature    11578..11758
                     /label="HIV LTR 1"
     rep_origin      complement(11802..76)
                     /label="ColE1 origin"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT
     1201 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT
     1261 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT
     1321 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG
     1381 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA
     1441 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
     1501 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
     1561 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
     1621 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
     1681 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA
     1741 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT
     1801 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
     1861 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA
     1921 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
     1981 GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT
     2041 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA
     2101 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA
     2161 GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG
     2221 AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC
     2281 GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT
     2341 GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA
     2401 GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG
     2461 AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA CAAATACTGG
     2521 GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA TATAATACAG
     2581 TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG GAAGCTTTAG
     2641 ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGCG GCCGGCCGCT
     2701 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
     2761 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT
     2821 GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC
     2881 AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGCCAGAC AATTATTGTC
     2941 TGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT GAGGCGCAAC AGCATCTGTT
     3001 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA
     3061 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC
     3121 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC
     3181 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT
     3241 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG
     3301 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT
     3361 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA
     3421 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG
     3481 ACCCGACAGG CCCGAAGGAA TAGAAGAAGA AGGTGGAGAG AGAGACAGAG ACAGATCCAT
     3541 TCGATTAGTG AACGGATCGG CACTGCGTGC GCCAATTCTG CAGACAAATG GCAGTATTCA
     3601 TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG
     3661 ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA
     3721 ATTTTCGGGT TTATTACAGG GACAGCAGAG ATCCAGTTTG GTTAGTACCG GGCCGGTGGA
     3781 ATTCTGCAGA TATCAACAAG TTTGTACAAA AAAGCAGGCT tcgagtggca cgtatactga
     3841 gtcattaggg actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa
     3901 caggaaagtc ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg
     3961 cccagtacaa aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac
     4021 ataaggtcaa taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt
     4081 actttcccac cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg
     4141 tactttccca tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg
     4201 aagtgaaagg gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg
     4261 gcacgcattc tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg
     4321 gtaccgtcgc agtcttcggt ctgaccaccg tagaacgcag aGTgaTccac cggggacgcg
     4381 aggactgtcc tcgccgccgt cgcgggcagt gtctagccag gccttgacaa gctagccgga
     4441 ggagcgccta ggaacccgag ccggagctca gcgagcgcag cctgcagctc ccgcctcgcc
     4501 gtcccggggg gcgtcccgcc tcccaccccg cctctggact tgtctctttc tccgcgcgcg
     4561 cggacacagc cggcgcttag gcccgagcga gcccgggggc cgccggccgg gaagacaacg
     4621 cgggcaccga ttcgccatgg agggcgccgg cggcgagaac gagaagaaaa agatgagttc
     4681 tgaacgtcga aaagaaaagt ctagagatgc agcaagatct cggcgaagca aagagtctga
     4741 agttttttat gagcttgctc atcagttgcc acttccccac aatgtgagct cacatcttga
     4801 taaagcttct gttatgaggc tcaccatcag ttatttacgt gtgagaaaac ttctggatgc
     4861 cggtggtcta gacagtgaag atgagatgaa ggcacagatg gactgttttt atctgaaagc
     4921 cctagatggc tttgtgatgg tgctaacaga tgacggcgac atggtttaca tttctgataa
     4981 cgtgaacaaa tacatggggt taactcagtt tgaactaact ggacacagtg tgtttgattt
     5041 tactcatcca tgtgaccatg aggaaatgag agaaatgctt acacacagaa atggcccagt
     5101 gagaaaaggg aaagaactaa acacacagcg gagctttttt ctcagaatga agtgcaccct
     5161 aacaagccgg gggaggacga tgaacatcaa gtcagcaacg tggaaggtgc ttcactgcac
     5221 gggccatatt catgtctatg ataccaacag taaccaacct cagtgtgggt acaagaaacc
     5281 acccatgacg tgcttggtgc tgatttgtga acccattcct catccgtcaa atattgaaat
     5341 tcctttaGAC agcaagacat ttctcagtcg acacagcctc gatatgaaat tttcttactg
     5401 tgatgaaaga attactgagt tgatgggtta tgagccggaa gaacttttgg gccgctcaat
     5461 ttatgaatat tatcatgctt tggattctga tcatctgacc aaaactcacc atgatatgtt
     5521 tactaaagga caagtcacca caggacagta caggatgctt gccaaaagag gtggatatgt
     5581 ctgggttgaa actGAAgcaa ctgtcatata taatacgaag aactcccagc cacagtgcat
     5641 tGAAtgtgtg aatACTgttg taagtggtat tattcagcac gacttgattt tctcccttca
     5701 acaaacagaa tctgtgctca aaccagttga atcttcagat aagaagatga ctcagctgtt
     5761 caccaaagtt gaatcagagg ctacaagctg cctttttgat aagcttaaga aggagcctga
     5821 tgctctcact ctgctggctc cagctgccgg cgacaccatc atctctctgg attttggcag
     5881 cgatgacaca gaaGGTTCCG GCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCTCTCT
     5941 CCCAGCGACA CATGAGTTAC ACATCTTTGG CTCCATCAAC GGTGTGGACT TTGACATGGT
     6001 GGGTCAGGGC ACCGGCAATC CAAATGATGG TTATGAGGAG TTAAACCTGA AGTCCACCAA
     6061 GGGTGACCTC CAGTTCTCCC CCTGGATTCT GGTCCCTCAT ATCGGGTATG GCTTCCATCA
     6121 GTACCTGCCC TACCCTGACG GGATGTCGCC TTTCCAGGCC GCCATGGTAG ATGGCTCCGG
     6181 CTACCAAGTC CATCGCACAA TGCAGTTTGA AGATGGTGCC TCCCTTACTG TTAACTACCG
     6241 CTACACCTAC GAGGGAAGCC ACATCAAAGG AGAGGCCCAG GTGAAGGGGA CTGGTTTCCC
     6301 TGCTGACGGT CCTGTGATGA CCAACTCGCT GACCGCTGCG GACTGGTGCA GGTCGAAGAA
     6361 GACTTACCCC AACGACAAAA CCATCATCAG TACCTTTAAG TGGAGTTACA CCACTGGAAA
     6421 TGGCAAGCGC TACCGGAGCA CTGCGCGGAC CACCTACACC TTTGCCAAGC CAATGGCGGC
     6481 TAACTATCTG AAGAACCAGC CGATGTACGT GTTCCGTAAG ACGGAGCTCA AGCACTCCAA
     6541 GACCGAGCTC AACTTCAAGG AGTGGCAAAA GGCCTTTACC GATGTGATGG GCATGGACGA
     6601 GCTGTACAAG GGCTCAGGAa ctgaagatca acaacttgaa gatgttccat tatataatga
     6661 tgtaatgttt ccctcttcta atgaaaaatt aaatataaac ctggcaatgt ctcctttacc
     6721 ttcatcggaa actccaaagc cacttcgaag tagtgctgat cctgcaCTTa atcaagaggt
     6781 tgcattaaaa ttagaatcaa gtccagagtc actgggactt tcttttacca tgccccagat
     6841 tcaagatcag ccagcaagtc cttctgatgg aagcactaga caaagttcac ctgagagact
     6901 tcttcaggaa aacgtaaaca ctcctaactt ttcccagcct aacagtccca gtgaatattg
     6961 ctttgatgtg gatagcgata tggtcaatgt attcaagttg gaactggtgg aaaaactgtt
     7021 tgctgaagac acagaggcaa agaatccatt ttcaactcag gacactgatt tagacttgga
     7081 gatgctggct ccctatatcc caatggatga tgatttccag ttacgttcct ttgatcagtt
     7141 gtcaccatta gagagcaatt ctccaagccc tccaagtatg agcacagtta ctgggttcca
     7201 gcagacccag ttacagaaac ctaccatcac tgccactgcc accacaactg ccaccactga
     7261 tgaatcaaaa acagagacga aggacaataa agaagatatt aaaatactga ttgcatctcc
     7321 atcttctacc caagtacctc aagaaacgac cactgctaag gcatcagcat acagtggcac
     7381 tcacagtcgg acagcctcac cagacagagc aggaaagaga gtcatagaac agacagacaa
     7441 agctcatcca aggagcctta acctgtctgc cactttgaat caaagaaata ctgttcctga
     7501 ggaagaatta aacccaaaga caatagcttc gcagaatgct cagaggaagc gaaaaatgga
     7561 acatgatggc tccctttttc aagcagcagg aattggaaca ttattgcagc aaccgggtga
     7621 ctgtgcacct actatgtcac tttcctggaa acgagtgaaa ggattcatat ctagtgaaca
     7681 gaatggaacg gagcaaaaga ctattatttt aataccctcc gatttagcat gcagactgct
     7741 ggggcagtca atggatgaga gtggattacc acagGCAacc agttacgatt gtgaagttaa
     7801 tgctcccata caaggcagca gaaacctact gcagggtgaa gaattactca gagctttgga
     7861 tcaagttaac GGTGGCGGTG GCAGCGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA
     7921 GGCTGGAGAC GTGGAGGAGA ACCCTGGACC Tatgccagag ccagcgaagt ctgctcccgc
     7981 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     8041 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     8101 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     8161 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     8221 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     8281 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaaga acatggggca
     8341 tggcaccggc agcaccggca gcggcagctc atccggaact agtatggtga gcaagggcga
     8401 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     8461 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     8521 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgAC
     8581 Ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     8641 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     8701 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     8761 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc
     8821 catcAGCGAT aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     8881 cAAAATTAGA CACaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     8941 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     9001 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     9061 cgccgccggg atcactctcg gcatggacga gctgtacaag AAGCTTAGCC ATGGCTTCCC
     9121 GCCGGAGGTG GAGGAGCAGG ATGATGGCAC GCTGCCCATG TCTTGTGCCC AGGAGAGCGG
     9181 GATGGACCGT CACCCTGCAG CCTGTGCTTC TGCTAGGATC AATGTGTaaG CGGCCGCACT
     9241 CGAGATATCT AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCCAGCAC AGTGGCGGCC
     9301 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT
     9361 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC
     9421 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA
     9481 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC
     9541 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT
     9601 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG
     9661 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT
     9721 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC
     9781 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG
     9841 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA
     9901 CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG
     9961 CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG
    10021 TCACACTCAG GTACAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC
    10081 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC
    10141 ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC
    10201 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCCGC CCCGCAGCTC GCGTCGTGCA
    10261 GGACGTGACA AATGGAAGTA GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG
    10321 AGCAATGGAA GCGGGTAGGC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT
    10381 TCTGGGCTCA GAGGCTGGGA AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG
    10441 GCGGGGCGGG CGCCCGAAGG TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA
    10501 CGTCTGCCGC GCTGTTCTCC TCTTCCTCAT CTCCGGGCCT TTCGACCTGC ATCCATCTAG
    10561 ATCTCGATCG AGCAGCTGAA GCTTACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC
    10621 ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC
    10681 GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA
    10741 CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC
    10801 GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC
    10861 GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC
    10921 CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG
    10981 CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC
    11041 GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC
    11101 GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG
    11161 TGCATGACCC GCAAGCCCGG TGCCTGAGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT
    11221 GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA
    11281 CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG
    11341 CAGAACTACA CACCAGGGCC AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG
    11401 CTAGTACCAG TTGAGCAAGA GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG
    11461 TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG
    11521 TTTGACAGCC GCCTAGCATT TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG
    11581 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG
    11641 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT
    11701 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC
    11761 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
    11821 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
    11881 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
    11941 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
    12001 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
    12061 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
    12121 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
    12181 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
    12241 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
    12301 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
    12361 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAA
//