LOCUS       Exported                7907 bp DNA     circular SYN 07-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   UNIL
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             576..1092
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1156..1497
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1564..1980
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1993..1998
                     /label=Kozak seq
     misc_feature    1999..3684
                     /label=ASNS
     primer_bind     2478..2497
                     /label=ASNS-seq1-F
     primer_bind     3059..3079
                     /label=ASNS-seq2-F
     primer_bind     complement(3654..3693)
                     /label=ASNS-OE-R
     misc_feature    3732..4285
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4286..4821
                     /label=Thy1.1
     LTR             4877..5391
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5553..5569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5577..5607)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5622..5643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5931..6519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6690..7550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7551..7655)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
       61 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
      121 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
      181 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg
      241 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
      301 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
      361 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
      421 cgtccggcgt agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct
      481 ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa
      541 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
      601 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag
      661 agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc
      721 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
      781 ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
      841 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
      901 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
      961 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
     1021 aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg
     1081 gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc
     1141 cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt
     1201 gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt
     1261 atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga
     1321 cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg
     1381 aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
     1441 cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag
     1501 cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1561 tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
     1621 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     1681 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     1741 atcacccagg ttaagatcaa ggtgttttca cctggcccgc atggacaccc agaccaggtc
     1801 ccctacatcg tgaccgggaa agccttggct tttgaccccc ctccctgggt caagcccttt
     1861 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
     1921 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
     1981 ggaattagat ctgccaccat gtgtggcatc tgggccctct tcggcagcga tgactgcctt
     2041 tccgtgcagt gtctgagtgc gatgaagatc gcgcacaggg ggccagatgc atttcgcttt
     2101 gagaatgtca atggatacac caactgctgc tttggctttc accgcttggc tgtggttgac
     2161 ccgctgtttg gaatgcagcc gataagagtg aggaaatacc cttatttgtg gctctgttac
     2221 aatggtgaaa tctacaacca caaggcgcta cagcaacgtt ttgaatttga atatcagacc
     2281 aatgtggatg gtgagattat cctccacctc tatgacaaag gaggcatcga gaaaaccatc
     2341 tgtatgctgg acggggtgtt tgcattcatc ttactggaca ctgccaataa gaaagtattt
     2401 ctgggcagag acacctatgg agtcaggccc ttgtttaaag ccatgacaga agatgggttt
     2461 ctggctgtgt gttcagaagc taaaggcctt gtttccttaa aacactccac cactcccttc
     2521 ttaaaagtgg agcccttcct tcctggacac tatgaagttt tggatttaaa accaaatggc
     2581 aaagttgcgt ctgtggaaat ggtcaaatac catcactgta cggatgaacc attgcatgcc
     2641 atctatgaca gcgtggagaa actcttccca ggctttgacc tagagaccgt gaagaacaat
     2701 ctgcgtatcc tttttgacaa cgctatcaag aaacgcttga tgacagaccg gaggattggc
     2761 tgccttttat cagggggcct ggactcgagc ttggttgctg cctctctgct gaagcaactc
     2821 aaggaggccc aagttcagta tcctctccag acatttgcta ttggcatgga ggacagcccc
     2881 gatctcctgg ccgctagaaa ggtggcaaat tatattggaa gcgagcatca tgaagtcctt
     2941 tttaactctg aagaaggcat tcaggccctg gatgaagtca tattttcctt ggaaacttat
     3001 gatattacga cagttcgggc atctgtgggc atgtatttaa tttccaagta tattcggaag
     3061 aacacagaca gcgtggtgat cttctccgga gaggggtcag atgaacttac acagggctat
     3121 atatatttcc acaaggctcc ttcccctgag aaggccgagg aggagagtga gagactgctg
     3181 aaggaactct acctgtttga tgttctccgg gccgaccgca ctactgctgc acacggtctc
     3241 gaactgagag tcccctttct ggatcatcgg ttttcttcct attacctgtc tctgccgcca
     3301 gatatgagaa ttccaaaaaa tggcatagaa aaacatctcc tgagagagac ttttgaggac
     3361 tgcaacctgc tacccaaaga gattctctgg cgacccaaag aagccttcag tgatgggatc
     3421 acctcagtca agaactcctg gttcaagatt ttgcaggact atgttgaaca tcaggttgat
     3481 gatgagatga tgtctgcagc ctcccagaag tttcccttca atactcccaa aactaaggaa
     3541 ggctacttct accgtcagat ctttgaacgc cattacccag gccgggctga ttggctgact
     3601 cattattgga tgcctaagtg gatcaatgct actgaccctt ctgcccgcac tctgacccat
     3661 tataagtcag ctgccaaagc ttaggtcgac ggtatcgata agcttgatat cgaattccgc
     3721 ccccccccct aacgttactg gccgaagccg cttggaataa ggcacggtgt gcgtttgtct
     3781 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     3841 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     3901 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     3961 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     4021 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4081 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     4141 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     4201 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     4261 tcctttgaaa aacacgatga taatatggcc acaaccatgg gtcttttctg cagtcaccgt
     4321 cctcgaggca ccatgaaccc agccatcagc gtcgctctcc tgctctcagt cttgcaggtg
     4381 tcccgagggc agaaggtgac cagcctgaca gcctgcctgg tgaaccaaaa ccttcgcctg
     4441 gactgccgcc atgagaataa caccaaggat aactccatcc agcatgagtt cagcctgacc
     4501 cgagagaaga ggaagcacgt gctctcaggc acccttggga tacccgagca cacgtaccgc
     4561 tcccgcgtca ccctctccaa ccagccctat atcaaggtcc ttaccctagc caacttcacc
     4621 accaaggatg agggcgacta cttttgtgag cttcgcgtct cgggcgcgaa tcccatgagc
     4681 tccaataaaa gtatcagtgt gtatagagac aagctggtca agtgtggcgg cataagcctg
     4741 ctggttcaga acacatcctg gatgctgctg ctgctgcttt ccctctccct cctccaagcc
     4801 ctggacttca tttctctgtg acgcgtagaa ttcgataaaa taaaagattt tatttagtct
     4861 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     4921 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta
     4981 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     5041 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     5101 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     5161 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     5221 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg
     5281 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5341 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag
     5401 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac
     5461 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga
     5521 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac
     5581 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5761 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5821 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6361 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6421 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6481 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6541 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6601 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6661 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7561 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7681 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7741 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     7801 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     7861 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatc
//
