LOCUS       Exported                6982 bp ds-DNA     circular SYN 06-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pS238D1::sptse5-CT.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=Geneious name: source synthetic DNA construct
                     /note="Geneious name: source synthetic DNA construct"
     CDS             complement(19..984)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in
                     the presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /note="Geneious name: xylS CDS"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        1884..1964
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
                     /note="Geneious name: Pm promoter"
     CDS             2017..3099
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Geneious name: lacI CDS"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    3121^3122
                     /label=Geneious type: Editing History Deletion
                     /note="Geneious type: Editing History Deletion"
                     /note="Geneious name: 
                     GTGGTGATGATAATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGC
                     TGCCCAGCCGGCGATGGCCATGGGA"
     CDS             3125..3574
                     /codon_start=1
                     /label=Tse5-CT
                     /note="Geneious name: Tse5-CT"
                     /translation="IIPLVVIGAFAARAAIGAALGAGIELGMQTGKQVLGQMKDNWDSD
                     RDLTDIKWKCIDINWKHVGASAAIGTVAPGMLSTGKTVVQSAKAIRTLSGQAANTANRA
                     AKLAARKAAHADTIKKAVATQAAWQTGKQIVKCPLKDEEEECPPQ"
     CDS             3581..3598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="Geneious name: 6xHis affinity tag CDS"
                     /translation="HHHHHH"
     terminator      complement(3715..3742)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="Geneious name: T7Te terminator"
     terminator      complement(3758..3828)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /note="Geneious name: rrnB T1 terminator"
     terminator      4014..4108
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
                     /note="Geneious name: lambda t0 terminator"
     CDS             4240..5055
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="Geneious name: aminoglycoside phosphotransferase
                     CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    5222..5330
                     /label=Geneious type: oriT
                     /note="Geneious type: oriT"
                     /note="incP origin of transfer"
                     /note="Geneious name: incP origin of transfer oriT"
     CDS             complement(5341..6003)
                     /codon_start=1
                     /product="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /note="Geneious name: replication protein for the 
                     broad-host-range  plasmid pBBR1 from Bordetella 
                     bronchiseptica CDS"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      6004..6775
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
                     /note="Geneious name: pBBR1 oriV"
     terminator      complement(6890..6976)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /note="Geneious name: Escherichia coli rrnB terminator"
ORIGIN
        1 ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg tgtcggaagg
       61 caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta agaagccgta
      121 gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca agcaggcgcg
      181 gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc
      241 gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc gctcaaggct
      301 gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag acgggttacc
      361 tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct tggaagcgat
      421 gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg cttcgtcaca
      481 aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc gcgcggcgaa
      541 acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat caaggaccac
      601 tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt cggcttggtc
      661 atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc catggccacg
      721 ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta atccaggcga
      781 gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc cgaagattct
      841 gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac
      901 atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga tctgactttt
      961 ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt gggctgcttg
     1021 gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg cgtatacggg
     1081 ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt ttccaagcaa
     1141 aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc agaagttcgt
     1201 gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga tattacggcc
     1261 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1321 gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg
     1381 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1441 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1501 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1561 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1621 gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct gaccaacgac
     1681 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac
     1741 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc
     1801 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaagtg
     1861 cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca ggagtgcaaa
     1921 aaatggctat ctctagtaag gcctacccct taggctttat gcaacagaaa caataataat
     1981 ggagtcatga ccatgcctag gaggaaaaaa catatggtga aaccagtaac gttatacgat
     2041 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     2101 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
     2161 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
     2221 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
     2281 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     2341 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     2401 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     2461 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     2521 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     2581 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     2641 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     2701 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     2761 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     2821 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     2881 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     2941 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     3001 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3061 ctggcacgac aggtttcccg actggaaagc gggcagtaac atcaccacca tcattaggat
     3121 gctgatcatc ccgctggtgg tgatcggcgc cttcgccgcc cgcgccgcga tcggagcggc
     3181 gctgggcgcc ggcatcgagc tggggatgca gaccggcaag caggtgctgg ggcagatgaa
     3241 ggacaactgg gacagcgatc gcgacctcac cgatatcaag tggaagtgca tcgacatcaa
     3301 ctggaaacac gtcggcgcct cggcggccat cggcaccgtg gcgccgggca tgctcagcac
     3361 cggcaagacc gtggtgcagt ccgccaaggc gatcaggacc ctttccggac aggcggccaa
     3421 caccgccaac cgcgcggcca agctggccgc gcgcaaggcc gcccatgccg ataccatcaa
     3481 gaaggccgtg gccacccagg ccgcctggca gacgggcaag cagatcgtca aatgcccact
     3541 gaaggacgaa gaagaggagt gtccgccgca atgactcgag caccaccacc accaccactg
     3601 aggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttgcgg ccgcgtcgtg
     3661 actgggaaaa ccctggcgca aagcccgccg aaaggcgggc ttttctgtta taaacgcaga
     3721 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaaaaca
     3781 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggctcg
     3841 agctcgagaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     3901 agtggcaatg caatggccca acagaaaatg gtgaaaccag taacgttata ctgaatctaa
     3961 gtatcaatgt gagcgctcac aagtcaacac tctttttgat aaaactagtc ttggactcct
     4021 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     4081 ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg atttaaattt
     4141 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     4201 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcagcgtgaa
     4261 acgagctgta gccgtccgcg tctgaacagc aacatggatg cggatctgta tggctataaa
     4321 tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt atcgtctgta tggcaaaccg
     4381 gatgcgccgg aactgtttct gaaacatggc aaaggcagcg tggcgaacga tgtgaccgat
     4441 gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc tgccgaccat taaacatttt
     4501 attcgcaccc cggatgatgc gtggctgctg accaccgcga ttccgggcaa aaccgcgttt
     4561 caggtgctgg aagaatatcc ggatagcggc gaaaacattg tggatgcgct ggccgtgttt
     4621 ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt ttaacagcga tcgtgtgttt
     4681 cgtctggccc aggcgcagag ccgtatgaac aacggcctgg tggatgcgag cgattttgat
     4741 gatgaacgta acggctggcc ggtggaacag gtgtggaaag aaatgcataa actgctgccg
     4801 tttagcccgg atagcgtggt gacccacggc gattttagcc tggataacct gattttcgat
     4861 gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg gcattgcgga tcgttatcag
     4921 gatctggcca ttctgtggaa ctgcctgggc gaatttagcc cgagcctgca aaaacgtctg
     4981 tttcagaaat atggcattga taatccggat atgaacaaac tgcaatttca tctgatgctg
     5041 gatgaatttt tctaataatt aattggaccg cggtccgcgc gttgtccttt tccgctgcat
     5101 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     5161 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     5221 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     5281 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg taggccggcc
     5341 ctaccggcgc ggcagcgtta cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa
     5401 acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc
     5461 ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc
     5521 gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc
     5581 gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa
     5641 caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta
     5701 ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa
     5761 gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt
     5821 ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat
     5881 tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt
     5941 ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc
     6001 catgcttatc tccatgcggt aggggtgccg cacggttgcg gcaccatgcg caatcagctg
     6061 caacttttcg gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga
     6121 ttttctttaa cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc
     6181 cccctttttt aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt
     6241 ggtgcccaaa gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc
     6301 cggcaaaaag tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca
     6361 aggtggcgct gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg
     6421 ggcgggcttc gcccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc
     6481 aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca
     6541 accggcaagc gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa
     6601 aattgatggg gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc
     6661 tgggtagccg gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca
     6721 gcttgtccag cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg
     6781 gccatcgtcc acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc
     6841 cggagagcaa gcccgtaggg ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     6901 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     6961 cctttcgttt tatttgatgc ct
//
