LOCUS       pJLG039                11397 bp DNA     circular SYN 06-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Jen
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11397
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        37..67
                     /label=PLac
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             137..147
                     /codon_start=1
                     /label=lacZ-alpha
                     /translation="I*I"
     misc_feature    160
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     gene            complement(166..2817)
                     /pseudo
                     /gene="int"
                     /locus_tag="ECD_10001"
                     /label=int
                     /note="inactivated by the insertion of T7 RNA
                     polymerasegene; similar to lambdap33 (integration protein)"
     gene            166..2817
                     /gene="1"
                     /locus_tag="ECD_10004"
                     /label=1
     CDS             166..2817
                     /codon_start=1
                     /transl_table=11
                     /gene="1"
                     /locus_tag="ECD_10004"
                     /product="T7 DNA-directed RNA polymerase"
                     /label=1
                     /protein_id="ACT42586.1"
                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE
                     MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ
                     FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK
                     KNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIE
                     STGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGI
                     TGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANV
                     ITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLE
                     FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYY
                     WLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
                     YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAK
                     KVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM
                     TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE
                     AMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG
                     QFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD
                     SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN
                     LRDILESDFAFA"
     misc_feature    2823..2862
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(2876..3313)
                     /codon_start=1
                     /label=tetR_trun
                     /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV
                     QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQAPFAIQA
                     AQLLGRAIGAGLFAITPAGERGMCCKAIKLGNARVFPVTTL"
     CDS             3392..4591
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=tetA
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     promoter        4650..4727
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4728..5810
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             complement(7156..8304)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8576..8947)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            8980..9089
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     rep_origin      9242..9873
                     /label=IncP-oriV
                     /note="incP origin of replication"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgaattc atcgatatct agatctctcg agctcgcgaa agcttatgaa cacgattaac
      181 atcgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa cactctggct
      241 gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga gtcttacgag
      301 atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg
      361 gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat tgcacgcatc
      421 aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc cttccagttc
      481 ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac tctggcttgc
      541 ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt
      601 gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt caagaaaaac
      661 gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt tatgcaagtt
      721 gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc ttcgtggcat
      781 aaggaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattga gtcaaccgga
      841 atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga gactatcgaa
      901 ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc tggcatctct
      961 ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac tggtggtggc
     1021 tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa gaaagcactg
     1081 atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat tgcgcaaaac
     1141 accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac caagtggaag
     1201 cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat gaaaccggaa
     1261 gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc tgctgtgtac
     1321 cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg agttcatgct tgagcaagcc
     1381 aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg gcgcggtcgt
     1441 gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg actgcttacg
     1501 ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat ccacggtgca
     1561 aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat tgaggaaaac
     1621 cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg ggctgagcaa
     1681 gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca gcaccacggc
     1741 ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgg catccagcac
     1801 ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttaacttgct tcctagtgaa
     1861 accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct acaagcagac
     1921 gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac tggtgaaatc
     1981 tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc ttacggtgtt
     2041 actcgcagtg tgactaagcg ttcagtcatg acgctggctt acgggtccaa agagttcggc
     2101 ttccgtcaac aagtgctgga agataccatt cagccagcta ttgattccgg caagggtctg
     2161 atgttcactc agccgaatca ggctgctgga tacatggcta agctgatttg ggaatctgtg
     2221 agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc tgctaagctg
     2281 ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg ttgcgctgtg
     2341 cattgggtaa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc tattcagacg
     2401 cgcttgaacc tgatgttcct cggtcagttc cgcttacagc ctaccattaa caccaacaaa
     2461 gatagcgaga ttgatgcaca caaacaggag tctggtatcg ctcctaactt tgtacacagc
     2521 caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta cggaatcgaa
     2581 tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc gaacctgttc
     2641 aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact ggctgatttc
     2701 tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc agcacttccg
     2761 gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt cgcgtaactg
     2821 caggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca
     2881 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2941 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3001 cagcctgaat ggcgaatggc gcctgcctga agctgcgggc attcccgatc agaaatgagc
     3061 gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg
     3121 ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa
     3181 cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca
     3241 ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat
     3301 cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc
     3361 ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacatacccc tgatcgtaat
     3421 tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg
     3481 cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc
     3541 gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt
     3601 cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact acgccatcat
     3661 ggcgacagcg cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg
     3721 ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc
     3781 gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct
     3841 cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa
     3901 cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg
     3961 gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac
     4021 cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc
     4081 cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat
     4141 ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt
     4201 agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg
     4261 ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct
     4321 tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga
     4381 ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt
     4441 cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc
     4501 atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg
     4561 gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa cgataggttg caattcgcgc
     4621 gcgaaggcga agcggcatgc atttacgttg acaccatcga atggtgcaaa acctttcgcg
     4681 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
     4741 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     4801 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     4861 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     4921 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     4981 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     5041 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     5101 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     5161 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
     5221 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     5281 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     5341 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     5401 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     5461 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     5521 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
     5581 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     5641 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     5701 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5761 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5821 attaatgtaa gttagcgcga attgcaacct atgccatgcg ggtcaaggcg acttccggca
     5881 agctatacgc gccctaggag tgcggttgga acgttggccc agccagatac tcccgatcac
     5941 gagcaggacg ccgatgattt gaagcgcact cagcgtctga tccaagaaca accatcctag
     6001 caacacggcg gtccccgggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg
     6061 cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc
     6121 caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac
     6181 tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac
     6241 gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag
     6301 gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac
     6361 gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag
     6421 agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc
     6481 gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc
     6541 gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac
     6601 gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc ggcctcgctg
     6661 ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg ggccgtcctc
     6721 ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccgc tcctgtttga
     6781 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
     6841 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
     6901 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
     6961 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
     7021 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
     7081 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
     7141 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     7201 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     7261 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     7321 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     7381 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     7441 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     7501 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     7561 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     7621 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     7681 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     7741 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     7801 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     7861 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     7921 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     7981 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     8041 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
     8101 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     8161 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     8221 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     8281 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     8341 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
     8401 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
     8461 ccgaatcttg ccctgcacga ataccagctc cgcgaagtcg ctcttcttga tggagcgcat
     8521 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
     8581 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
     8641 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
     8701 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
     8761 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
     8821 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
     8881 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
     8941 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     9001 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     9061 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     9121 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
     9181 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgaccctttc
     9241 cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa
     9301 acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat
     9361 acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg
     9421 ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt
     9481 ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga
     9541 tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta
     9601 ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg
     9661 cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt
     9721 tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta
     9781 taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg
     9841 gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg
     9901 ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg caggacaact ggtgagtctg
     9961 gatctgaagt tcattgttgt catggatgat ctggaccgac tggagccatc ccaggtggcg
    10021 gaagtgttca ggcttgtgcg tgcagtagcc gatctgcccc gctttaccca tattctctgt
    10081 tatgacaggc agattatcac tcatgccgtt gaacatgcgc tgaatatcga agatggcagc
    10141 cgttatctcc agaaaatcat tcagcttagt tttaaattac cccgacctga agcctttgat
    10201 ttacgtaatg aatttcgcca gcgggctgag gctctatatc agcaaattaa taatcaaccg
    10261 ccagactctg gaatggtaag ggatctcatc gcggtgactg atacctatgg tgccgcactt
    10321 tcgacgccac gggaaatcca tcaggccatt aattccctga tttttcttta tccggggatg
    10381 cgggattttg tttatttccc tgatttgtgc ctgcttcagc ttatacgggt gacaaacccg
    10441 gctctgtatg actggacaga gcattacctg acagaacggt ccgtgattga aaccggtcag
    10501 ggtatgcttt ctgacggaga gaaagcagac ttccgggagg ggcttatcag atgtatgaag
    10561 acgttcaggg catcaaatgc agactcgttt ctgacacttg cagactggat ctatctcatc
    10621 tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac
    10681 gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag
    10741 aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa
    10801 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
    10861 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
    10921 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
    10981 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
    11041 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
    11101 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
    11161 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
    11221 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
    11281 ggtaggcgag cagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11341 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaat
//
