LOCUS       pAAV-mPGK-Chst3    6054 bp      DNA     circular   12/10/2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label=AAV ITR
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     Promoter        176..684
                     /label=PGK promoter
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    700..1190
                     /label=beta-globin intron
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     CDS             1256..2671
                     /label=Chst3
                     /SerialCloner_Color=&h803F02
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             2690..2719
                     /label=myc-tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             2738..2761
                     /label=3xFLAG
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     polyA_signal    2800..3280
                     /label=hGH poly(A) signal
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    3317..3457
                     /label=AAV ITR
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    3532..3987
                     /label=f1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4269..4373
                     /label=AmpR promoter
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4572..5231
                     /label=AmpR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5383..6011
                     /label=ColE1 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 
      121 AGGGGTTCCT GCGGCCGCAC GCGCGATCAC GAGACTAGCC TCGACGATGG TCGAGTACCG 
      181 GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG 
      241 CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG 
      301 CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG 
      361 GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG 
      421 TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG 
      481 GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG 
      541 TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC 
      601 CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC 
      661 CTCATCTCCG GGCCTTTCGA CCTCTAGCGG GATCGGATTC GAATCCCGGC CGGGAACGGT 
      721 GCATTGGAAC GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA 
      781 GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC 
      841 TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC 
      901 ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA 
      961 AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA 
     1021 CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT 
     1081 CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG 
     1141 GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTGGG ATTCGAACAT 
     1201 CGATTGAATT CGTCGACTGG ATCCGGTACC GAGGAGATCT GCCGCCGCGA TCGCCATGGA 
     1261 GAAAGGACTC GCTTTGCCTC AGGATTTCCG GGACCTTGTA CACAGCCTAA AGATTCGAGG 
     1321 CAGATACGTC TTGTTCCTGG CATTTGTGGT CATAGTTTTT ATCTTCATTG AAAAGGAAAA 
     1381 TAAAATCATA TCCAGGGTCT CCGACAAGCT GAAGCAGATC CCTCATTTTG TGGCAGATGC 
     1441 CAACAGCACT GACCCAGCCC TGCTCTTATC GGAGAATGCA TCTCTCTTGT CCCTGAGCGA 
     1501 GTTGGATTCC ACCTTTTCCC ATCTGCGGAG CCGCCTGCAC AACCTGAGCC TGCAGCTGGG 
     1561 CGTGGAGCCA GCAATGGAGA GCCAGGAGGC TGGGGCAGAG AAGCCATCCC AGCAGGCTGG 
     1621 AGCAGGGACC CGGCGCCACG TGCTTCTCAT GGCCACCACC CGCACGGGTT CCTCGTTCGT 
     1681 GGGCGAGTTC TTCAACCAGC AGGGCAATAT CTTCTACCTC TTCGAGCCAC TGTGGCACAT 
     1741 CGAGCGCACC GTGTTCTTCC AGCAGCGAGG CGCCAGCGCG GCTGGTTCAG CCTTGGTCTA 
     1801 CCGTGATGTC CTCAAGCAGT TGTTGCTATG CGACCTGTAT GTGCTGGAGC CCTTCATCAG 
     1861 CCCTCCGCCC GAGGACCACT TGACTCAGTT CCTGTTCCGC CGGGGATCCA GCCGTTCACT 
     1921 CTGCGAGGAT CCGGTGTGCA CACCCTTCGT CAAGAAGGTC TTTGAGAAGT ACCACTGCAG 
     1981 GAACCGTCGC TGCGGGCCAC TCAACGTGAC CTTGGCGGGC GAGGCCTGCC GCCGCAAGGA 
     2041 CCACGTGGCC CTCAAGGCTG TGCGCATCCG TCAGCTGGAG TTCCTGCAGC CGCTAGTTGA 
     2101 GGACCCGAGG TTGGATCTAC GAGTCATTCA GCTGGTGCGC GACCCCCGGG CCGTGCTGGC 
     2161 TTCACGCATA GTGGCCTTTG CGGGCAAGTA TGAGAACTGG AAGAAGTGGC TGTCCGAGGG 
     2221 GCAGGACCAG CTGAGCGAGG ATGAGGTGCA GCGATTGCGG GGCAACTGTG AGAGCATCCG 
     2281 CCTGTCTGCA GAGCTGGGCT TGCGGCAGCC AGCCTGGCTG CGCGGTCGTT ACATGCTGGT 
     2341 GCGCTATGAG GATGTGGCAC GCAGGCCACT GCAGAAGGCC CGAGAGATGT ACAGCTTTGC 
     2401 GGGCATCCCC TTGACCCCGC AGGTGGAGGA CTGGATCCAG AAGAACACGC AGGCGACACG 
     2461 CGACAGCAGC GATGTCTACT CCACTCAGAA AAACTCTTCT GAGCAGTTTG AGAAGTGGCG 
     2521 CTTCAGCATG CCTTTCAAGC TGGCACAGGT GGTACAGGCT GCCTGTGGCC CGACCATGCA 
     2581 CCTCTTTGGC TACAAGTTGG CCAGGGATGC CGCCTCACTC ACCAACCGCT CCATCAGCCT 
     2641 GCTGGAGGAG CGGGGCACCT TCTGGGTCAC GACGCGTACG CGGCCGCTCG AGCAGAAACT 
     2701 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     2761 GGTTTAAACG GCCGGCCAGC GCGCTGCTCG AGAGATCTAC GGGTGGCATC CCTGTGACCC 
     2821 CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT 
     2881 AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG 
     2941 GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT 
     3001 CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC 
     3061 CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT 
     3121 GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG 
     3181 GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG 
     3241 ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTGTAGG TAACCACGTG 
     3301 CGGACCGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT 
     3361 CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG 
     3421 GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG GTATTTTCTC 
     3481 CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT 
     3541 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     3601 CCAGCGCCTT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     3661 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     3721 GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     3781 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     3841 TCCAAACTGG AACAACACTC AACTCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT 
     3901 TGCCGATTTC GGTCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     3961 TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 
     4021 CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT 
     4081 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC 
     4141 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT 
     4201 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 
     4261 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 
     4321 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 
     4381 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 
     4441 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 
     4501 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 
     4561 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 
     4621 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 
     4681 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 
     4741 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 
     4801 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 
     4861 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 
     4921 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 
     4981 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 
     5041 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 
     5101 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 
     5161 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 
     5221 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 
     5281 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 
     5341 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 
     5401 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 
     5461 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 
     5521 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC 
     5581 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 
     5641 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 
     5701 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 
     5761 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 
     5821 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 
     5881 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 
     5941 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 
     6001 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGT
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