LOCUS       Exported                6884 bp ds-DNA    circular SYN 12-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pML2003
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 13, 2022 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    170..220
                     /note="P6"
     misc_feature    235..309
                     /note="riboj53"
     gene            344..1930
                     /note="prpR"
     terminator      1934..1978
                     /note="L3S3P41"
     rep_origin      1997..2821
                     /note="more stringent p15A"
     CDS             complement(2893..3708)
                     /codon_start=1
                     /note="Kanamycin phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    3809..3861
                     /label="L3S3P21"
                     /note="L3S3P21"
     misc_feature    3866..4074
                     /note="Pprp"
     misc_feature    4079..4153
                     /label="RiboJ"
                     /note="RiboJ"
     misc_feature    4154..4161
                     /label="SCAR"
                     /note="SCAR"
     misc_feature    4162..4173
                     /label="B0064"
                     /note="B0064"
     misc_feature    4174..4179
                     /label="SCAR(2)"
                     /note="SCAR"
     misc_feature    4180..4899
                     /label="YFP"
                     /note="YFP"
     misc_feature    4900..4960
                     /label="L3S2P21 Terminator"
                     /note="L3S2P21 Terminator"
     misc_feature    4970..5051
                     /label="Constitutive Promoter + RBS (DNA2.0 Vector)"
                     /note="Constitutive Promoter + RBS (DNA2.0 Vector)"
     misc_feature    5052..6134
                     /label="LacI(1)"
                     /note="LacI(1)"
     CDS             6153..6776
                     /codon_start=1
                     /label="TetR"
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    6777..147
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
ORIGIN
        1 caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
       61 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      121 cgggctcatg agcaaatatt ttatctgagg tgcttcctcg ctcacagagg ataagtccct
      181 aactttgaca atgcctagat caccgtatat aatgctagca ctgacgagac tacgagcggt
      241 caacgcatgt gctttgcgtt ctgatgagac agtgatgtcg aaaccgcctc tacaaataat
      301 tttgtttaac ccaatattcg gtttcttaac tttgcggtgc gctatggcac atccaccacg
      361 gcttaatgac gacaaaccgg ttatctggac ggtatctgta acgcgcctgt tcgagctgtt
      421 tcgcgatatc agcctcgagt ttgatcacct ggcgaacatt acccctatcc agcttggctt
      481 tgaaaaagcg gtgacctaca tccacaagaa actggcaaac gaacgctgtg acgccatcat
      541 cgccgctgga tctaacggcg cgtacctgaa aagccgcctg tcagtgccag ttattttgat
      601 taaaccgagc ggctacgatg tgttacaggc actggcaaaa gccggaaaac tcacctcttc
      661 tatcggcgtt gtcacttatc aggaaactat tccggcactg gtggcgtttc aaaaaacctt
      721 taatttgcgc ctcgatcaac gtagctacat taccgaagaa gatgcacgcg ggcagattaa
      781 cgagctaaaa gctaacggca ccgaagcggt ggtcggcgcg gggctgatta ccgatctggc
      841 agaagaagcc ggaatgaccg gaatttttat ctattccgcc gccaccgtgc gccaggcgtt
      901 cagcgatgcg ctggatatga cgcgcatgtc gttacgccat aacactcacg atgccacccg
      961 caacgccctg agaactcgtt acgtgctggg cgatatgctc ggtcaatcac cacagatgga
     1021 acaagtacgg cagactattt tgctgtatgc ccgctccagt gcagcggtgt tgattgaggg
     1081 ggaaacgggg acgggcaaag agctggcggc ccaggcgatt catcgggaat attttgcccg
     1141 ccacgatgcg cgacagggca aaaagtcgca tccgtttgtt gcagtcaact gcggggcgat
     1201 tgccgaatcg ctgctggaag cagaactgtt tggctatgag gaaggggcgt ttaccggctc
     1261 gcgacgcggc ggtcgcgccg ggctgtttga aatcgcccac ggaggtacgc tgtttctcga
     1321 tgagattggc gaaatgccgc tgccgttgca gacccggctg ctgcgggtgc tggaagaaaa
     1381 agaggtcacc cgcgtcggcg ggcatcagcc tgttccggtg gatgtgcggg tcattagcgc
     1441 cactcactgc aatctggaag aagatatgcg gcaagggcag tttcgccgtg acctgtttta
     1501 tcggctgagt attttgcgtc tgcaattgcc accactgcgc gagcgggtgg cggatattct
     1561 gccactggcg gaaagctttt tgaaagtgtc tctggcggcg ctctccaccc cgttttctgc
     1621 cgcattacgc cagggattac aggcaagcga aaccgtgctg gtgcactacg actggccggg
     1681 caatattcgt gaactgcgca atatgatgga gcgactggcg ctatttttaa gtgtggaacc
     1741 gacgccggat ttaacgccgc aatttttgca gctgctactg ccggaactgg cgcgcgagtc
     1801 ggcgaaaact cccgctccac gcttactgac accacaacag gcactggaga aatttaatgg
     1861 cgataaaaca gcagcggcga attatttagg catcagccgg acgacgttct ggcggcggct
     1921 gaaaagctga gtgaaaaaaa aaacacccta acgggtgttt tttttttttt ggtctcccat
     1981 cggcagacct cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     2041 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     2101 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     2161 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     2221 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     2281 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     2341 aagataccag gcgtttcccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     2401 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     2461 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     2521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     2581 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     2641 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     2701 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     2761 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     2821 aggggtctga cgctcagtgg aacgaaaaat caatctaaag tatatatgag taaacttggt
     2881 ctgacagtta ccttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     2941 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     3001 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     3061 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     3121 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     3181 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     3241 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     3301 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     3361 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     3421 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     3481 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     3541 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     3601 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     3661 atttaatcgc ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     3721 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     3781 aatgtacatc agagattttg agacacaacc aattattgaa ggcctcccta acggggggcc
     3841 tttttttgtt tctggtctcc cgcttgtctt gtttcataat tgttgcaatg aaacgcggtg
     3901 aaacattgcc tgaaacgtta actgaaacgc atatttgcgg attagttcat gactttatct
     3961 ctaacaaatt gaaattaaac atttaatttt attaaggcaa ttgtggcaca ccccttgctt
     4021 tgtctttatc aacgcaaata acaagttgat aacaagctag caggaggaat tcacctgaag
     4081 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa
     4141 taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa gggcgaggag
     4201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4321 atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     4381 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc
     4441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4741 ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta ccagtccgcc
     4801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4861 gccgggatca ctctcggcat ggacgagctg tacaagtaac tcggtaccaa attccagaaa
     4921 agaggcctcc cgaaaggggg gccttttttc gttttggtcc aaatgaatgg cggcgcgcca
     4981 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
     5041 gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     5101 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     5161 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     5221 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     5281 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     5341 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     5401 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     5461 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     5521 acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat
     5581 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     5641 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     5701 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     5761 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     5821 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     5881 aagatagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     5941 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     6001 agctgttgcc agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     6061 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6121 aaagcgggca gtgacaatcc aggaggaaaa aaatgtccag attagataaa agtaaagtga
     6181 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     6241 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     6301 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     6361 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     6421 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     6481 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     6541 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     6601 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     6661 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     6721 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcctaataat
     6781 tggtaacgaa tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg
     6841 cttcgtccat ttgacaggca cattatgcat cgatgataag ctgt
//
