LOCUS       Exported                7687 bp ds-DNA     circular SYN 13-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 13, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : Cytoplasm-Apollo-Venus.txt.xdna [6950  nt] : 
            (XhoI[3244] / AgeI[2511]) to PCR(mTurquoise2.txt.xdna) [729  nt] : 
            (AgeI[12] / XhoI[745])
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    819..836
                     /label=Decision Point
                     /note="color: #408080; direction: RIGHT"
     primer_bind     863..882
                     /label=T7
                     /note="color: #008b8b; direction: RIGHT"
     misc_feature    964..1080
                     /label=G6PD (delta PHKL) [*]
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
     misc_feature    1081
                     /label=S40A
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1082..1175
                     /label=G6PD (delta PHKL) [*] [*]
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
     misc_feature    1176
                     /label=G6PD (delta PHKL) [*] [*] [*]
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
     misc_feature    1176
                     /label=silent Mutation
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1178..1179
                     /label=R72Q
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1179
                     /label=G6PD (delta PHKL) [*] [*] [*] [5']
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
     misc_feature    1181..1563
                     /label=G6PD (delta PHKL) [*] [*] [*] [3'] [*]
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
     misc_feature    1564
                     /label=H201N
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1565..1576
                     /label=G6PD (delta PHKL) [*] [*] [*] [3'] [*]
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
     misc_feature    1577
                     /label=K205T
                     /note="a614c"
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1578..2508
                     /label=G6PD (delta PHKL) [*]
                     /note="note=""direct strand 
                     translation""translation=""MAEQVALSRTQVCGILREELFQGDAFHQSDTH
                     IFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
                     PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAV
                     TKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQ
                     "
                     /note="color: #804040; direction: RIGHT"
                     2516..3244
                     /note="note=""Fragment extracted from 
                     PCR(mTurquoise2.txt.xdna) [729  nt] : (AgeI[12] / 
                     XhoI[745])"
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    2522..3113
                     /label=G6PD [*]
                     /note="color: #800000; direction: RIGHT"
     misc_feature    2524..3243
                     /label=mTurquoise2
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    3114
                     /label=G6PD [*] [5']
                     /note="color: #800000; direction: RIGHT"
     misc_feature    3114
                     /label=R198P
                     /note="g593c"
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    3116..3259
                     /label=G6PD [*] [3']
                     /note="color: #800000; direction: RIGHT"
     terminator      3284..3511
                     /label=bGH PA
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    4179..4256
                     /label=SV40 ORI
                     /note="color: #4682b4; direction: RIGHT"
     CDS             4398..5189
                     /label=Kan/neoR
                     /note="color: #8fbc8f; direction: RIGHT"
     primer_bind     complement(5529..5549)
                     /label=M13-rev
                     /note="color: #008b8b; direction: LEFT"
     misc_binding    complement(5555..5577)
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     promoter        complement(5582..5611)
                     /label=lac
                     /note="color: #4682b4; direction: LEFT"
     CDS             complement(6694..7353)
                     /label=AmpR
                     /note="color: #8fbc8f; direction: LEFT"
     promoter        complement(7593..7621)
                     /label=Amp prom
                     /note="color: #4682b4; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggcag agcaggtggc cctgagccgg acccaggtgt gcgggatcct gcgggaagag
     1021 cttttccagg gcgatgcctt ccatcagtcg gatacacaca tattcatcat catgggtgca
     1081 gcgggtgacc tggccaagaa gaagatctac cccaccatct ggtggctgtt ccgggatggc
     1141 cttctgcccg aaaacacctt catcgtgggc tatgctcagt cccgcctcac agtggctgac
     1201 atccgcaaac agagtgagcc cttcttcaag gccaccccag aggagaagct caagctggag
     1261 gacttctttg cccgcaactc ctatgtggct ggccagtacg atgatgcagc ctcctaccag
     1321 cgcctcaaca gccacatgaa tgccctccac ctggggtcac aggccaaccg cctcttctac
     1381 ctggccttgc ccccgaccgt ctacgaggcc gtcaccaaga acattcacga gtcctgcatg
     1441 agccagatag gctggaaccg catcatcgtg gagaagccct tcgggaggga cctgcagagc
     1501 tctgaccggc tgtccaacca catctcctcc ctgttccgtg aggaccagat ctaccgcatc
     1561 gacaactacc tgggcacgga gatggtgcag aacctcatgg tgctgagatt tgccaacagg
     1621 atcttcggcc ccatctggaa ccgggacaac atcgcctgcg ttatcctcac cttcaaggag
     1681 ccctttggca ctgagggtcg cgggggctat ttcgatgaat ttgggatcat ccgggacgtg
     1741 atgcagaacc acctactgca gatgctgtgt ctggtggcca tggagaagcc cgcctccacc
     1801 aactcagatg acgtccgtga tgagaaggtc aaggtgttga aatgcatctc agaggtgcag
     1861 gccaacaatg tggtcctggg ccagtacgtg gggaaccccg atggagaggg cgaggccacc
     1921 aaagggtacc tggacgaccc cacggtgccc cgcgggtcca ccaccgccac ttttgcagcc
     1981 gtcgtcctct atgtggagaa tgagaggtgg gatggggtgc ccttcatcct gcgctgcggc
     2041 aaggccctga acgagcgcaa ggccgaggtg aggctgcagt tccatgatgt ggccggcgac
     2101 atcttccacc agcagtgcaa gcgcaacgag ctggtgatcc gcgtgcagcc caacgaggcc
     2161 gtgtacacca agatgatgac caagaagccg ggcatgttct tcaaccccga ggagtcggag
     2221 ctggacctga cctacggcaa cagatacaag aacgtgaagc tccctgacgc ctatgagcgc
     2281 ctcatcctgg acgtcttctg cgggagccag atgcacttcg tgcgcagcga cgagctccgt
     2341 gaggcctggc gtattttcac cccactgctg caccagattg agctggagaa gcccaagccc
     2401 atcccctata tttatggcag ccgaggcccc acggaggcag acgagctgat gaagagagtg
     2461 ggtttccagt atgagggcac ctacaagtgg gtgaaccccc acaagctcag accggtcgcc
     2521 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2581 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     2641 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2701 accctcgtga ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     2761 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2821 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2881 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2941 cacaagctgg agtacaacta ctttagcgac aacgtctata tcaccgccga caagcagaag
     3001 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     3061 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3121 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     3181 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3241 taactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3301 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3361 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3421 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3481 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3541 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3961 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4081 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4141 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4201 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4261 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4321 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4381 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4441 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4501 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4561 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4621 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4681 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4741 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4801 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4861 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4921 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4981 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5041 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5101 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5161 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5221 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5281 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5341 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5401 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5461 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5521 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5581 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5641 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6361 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     6481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7681 tgacgtc
//
