LOCUS       Exported                4902 bp DNA     circular SYN 21-SEP-2022
DEFINITION  Vector for fusing EGFP to the C-terminus of a partner protein. For 
            other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION   .
VERSION     .
KEYWORDS    Lifeact-Halo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Clontech
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2022 from SnapGene 6.0.6
            https://www.snapgene.com
COMMENT     VNTSEQSTYLE|629|693||0|null|null|null|null|128,0,255
                 VNTSEQSTYLE|707|1426||0|null|null|null|null|0,255,0
FEATURES             Location/Qualifiers
     source          1..4902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          676..1565
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        61..364
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
     CDS             603..653
                     /codon_start=1
                     /label=Lifeact
                     /note="/type=misc_feature"
                     /translation="MGVADLIKKFESISKEE"
     CDS             676..1565
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     polyA_signal    1690..1811
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1818..2273)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of 
                     replication;arrowindicates direction of (+) strand 
                     synthesis"
     promoter        2300..2404
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     promoter        2406..2763
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2614..2749
                     /label=SV40 ori
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2798..3592
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII)
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, 
                     andG418(Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3824..3871
                     /label=HSV TK poly(A) signal
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylationsignal"
     rep_origin      4200..4788
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     ofreplication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatgggcgt ggccgacttg atcaagaagt tcgagtccat ctccaaggag gagggggatc
      661 caccggtcgc caccatggca gaaatcggta ctggctttcc attcgacccc cattatgtgg
      721 aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc acccctgtgc
      781 tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc ccgcatgttg
      841 caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc gacaaaccag
      901 acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc gaagccctgg
      961 gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt ttccactggg
     1021 ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc cgccctatcc
     1081 cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc cgcaccaccg
     1141 acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg ctgccgatgg
     1201 gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc
     1261 ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc ggtgagccag
     1321 cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc cctgtcccga
     1381 agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg
     1441 ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtctgaat ctgctgcaag
     1501 aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtcgacg ctcgagattt
     1561 ccggctaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     1621 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     1681 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1801 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     1861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     1921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     1981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     2041 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2101 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2161 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2221 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     2281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2401 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     2461 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     2521 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2581 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2641 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     2701 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     2761 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     2821 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     2881 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     2941 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     3001 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3061 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3121 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3181 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3241 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3301 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     3361 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3421 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3481 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3541 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     3601 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     3661 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     3721 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     3781 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     3841 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     3901 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     3961 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4021 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     4081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4321 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4501 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4561 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4621 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4681 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4741 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4801 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4861 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
