LOCUS pHAGE2_TetOminiC 11320 bp ds-DNA circular 12-DEC-2017 SOURCE ORGANISM COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers LTR 1..636 /gene="LTR" /vntifkey="19" /note="Long Terminal Repeat" /label=5'\LTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(3939..4118,4122..4196) /label=HMG-Sox2 /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 637..1155 /gene="psi" /product="pbs-gag" /vntifkey="21" /note="PSI Packaging Sequence" /label=PSI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4119..4121 /label=HMG-Sox17 /ApEinfo_fwdcolor=#800080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1303..1536 /gene="rre" /product="minimal RRE" /vntifkey="21" /note="Rev Responsive Element" /label=RRE /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2034..2211 /gene="cppt" /vntifkey="21" /note="Central Poly Purine Track" /label=HIV\cpPu\(Trip) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 7761..8352 /gene="WPRE" /vntifkey="21" /note="Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element" /label=WPRE /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 8934..9773 /gene="AMPr" /product="beta-lactamase (mature form)" /vntifkey="4" /note="Ampicillin Resistance Gene" /label=AMPr /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 10002..10535 /gene="ORI" /product="ColE1 origin of replication" /vntifkey="33" /note="Bacterial Origin of Replication" /label=ORI /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10772..10895 /gene="SV40 ori" /vntifkey="21" /label=SV40\ori /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 11215..11243 /vntifkey="28" /label=pHAGE-S1-500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 401..421 /vntifkey="28" /label=pHAGE-S501-1000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 898..919 /vntifkey="28" /label=pHAGE-S1001-1500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 1391..1417 /vntifkey="28" /label=pHAGE-S1501-2000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 1898..1922 /vntifkey="28" /label=pHAGE-S2001-2500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 8020..8040 /vntifkey="28" /label=pHAGE-S4001-4500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 8710..8732 /vntifkey="28" /label=pHAGE-S6001-6500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 9183..9207 /vntifkey="28" /label=pHAGE-S6501-7000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 9703..9727 /vntifkey="28" /label=pHAGE-S7001-7500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 10207..10231 /vntifkey="28" /label=pHAGE-S7501-8000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 10710..10729 /vntifkey="28" /label=pHAGE-S8001-8500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(158..179) /vntifkey="28" /label=pHAGE-A0050-8050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(667..690) /vntifkey="28" /label=pHAGE-A0550-0050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1166..1189) /vntifkey="28" /label=pHAGE-A1050-0550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1650..1678) /vntifkey="28" /label=pHAGE-A1550-1050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(2084..2112) /vntifkey="28" /label=pHAGE-A2050-1550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(7867..7890) /vntifkey="28" /label=pHAGE-A3550-3050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(8983..9006) /vntifkey="28" /label=pHAGE-A6050-5550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(9452..9475) /vntifkey="28" /label=pHAGE-A6550-6050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(9963..9984) /vntifkey="28" /label=pHAGE-A7050-6550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(10474..10495) /vntifkey="28" /label=pHAGE-A7550-7050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(10950..10976) /vntifkey="28" /label=pHAGE-A8050-7550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 455..551 /vntifkey="21" /note="HIV Repeat Region" /label=HIV\R /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 636..658 /vntifkey="21" /note="tRNA binding site for Lysine tRNA" /label=PBS-K /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 697..806 /vntifkey="21" /label=HIV-PSI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 807..1144 /vntifkey="21" /note="HIV gag sequence 351bp to 688bp" /label=HR-ePSI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 552..636 /vntifkey="21" /note="HIV U5" /label=HIV\U5 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..454 /vntifkey="21" /note="Full Length HIV U3" /label=HIV\U3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 8881..8915 /vntifkey="30" /note="Beta Lactamase Promoter" /label=P-Bla /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(7789..7823) /vntifkey="28" /label=pHAGE\3'\CDS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8357..8458 /vntifkey="21" /note="HIV nef" /label=HIV\nef /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8499..8595 /vntifkey="21" /label=HIV\R(1) /ApEinfo_label=HIV\R /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8596..8678 /vntifkey="21" /note="HIV U5" /label=HIV\U5(1) /ApEinfo_label=HIV\U5 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8407..8421 /vntifkey="21" /note="PolyPurine Tract" /label=HIV\PPT /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 mutation 833..834 /vntifkey="62" /note="Filled in Cla Site from original HIV genome to disrupt gag polyprotein ORF" /label=Fill-in /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 LTR 8357..8678 /vntifkey="19" /label=3'\LTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 splicing_signal 744..745 /vntifkey="38" /note="Major 5' Splice Donor 289" /label=5'\SD\289 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 splicing_signal 1911..1912 /vntifkey="38" /label=SA\7925 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 splicing_signal 2188..2189 /vntifkey="38" /label=SA\4459 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 mutation 1357..1357 /vntifkey="62" /note="This G is a C in the WT HIV-1 genome" /label=RRE\Mutation /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2014..2027 /vntifkey="21" /note="Linker Junk Sequence probably left over from cloning" /label=Junk /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1156..2013 /vntifkey="4" /note="HIV env sequence from bp7168 to 8025 (contains RRE) Cloned from BglII to BamHI out of HIV" /label=env /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 790..1144 /vntifkey="4" /note="HIV gag sequence from 336bp to 688bp (Clone to PvuII site in HIV and filled in ClaI digest to take gag CDS out of frame)" /label=HIV\gag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 5'UTR 551..635 /vntifkey="52" /note="HIV 5' UTR" /label=5'\UTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 853..855 /vntifkey="21" /note="Stop codon in gag put in frame by ClaI fill in" /label=Stop\Codon /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1245..1593 /vntifkey="21" /note="Rev Responsive Element according to Belasco in Molecular Cell, Vol. 7, 603-614, March, 2001" /label=RRE(1) /ApEinfo_label=RRE /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8424..8460 /vntifkey="21" /note="1st Half of deleted U3" /label=dU3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8480..8498 /vntifkey="21" /note="2nd Half of deleted U3" /label=dU3(1) /ApEinfo_label=dU3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 697..731 /vntifkey="39" /note="PSI Stem loop 1" /label=SL-1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 736..754 /vntifkey="39" /note="PSI Stem loop 2" /label=SL-2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 766..779 /vntifkey="39" /note="PSI Stem Loop 3" /label=SL-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 793..806 /vntifkey="39" /note="PSI Stem Loop 4" /label=SL-4 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_site 550..550 /vntifkey="26" /note="Exact base to which Poly-A is added" /label=pA\Site /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 570..577 /vntifkey="25" /note="GT Cluster shown to be necessary for Poly-A site activity by Cullen in J Virol. 1989 January; 63(1):421-424" /label=G-T\cluster /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 2556..2675 /vntifkey="29" /label=miniCMV /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2238..2555 /vntifkey="21" /note="Tetracyclin responsive element (TRE)" /label=TetO /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8065..8144 /label=WPRE(1) /ApEinfo_label=WPRE /ApEinfo_fwdcolor=#a5c4b3 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 9871..10553 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 11016..11038 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 9114..9773 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8065..8144 /label=WPRE(2) /ApEinfo_label=WPRE /ApEinfo_fwdcolor=#a5c4b3 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2062..2076 /label=cPPT /ApEinfo_fwdcolor=#a596b4 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8407..8421 /label=cPPT(1) /ApEinfo_label=cPPT /ApEinfo_fwdcolor=#a596b4 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3075..3299 /label=Oct4-POUsp /ApEinfo_fwdcolor=#4d8222 /ApEinfo_revcolor=#4d8222 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3300..3350 /label=Oct4-linker /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3351..3530 /label=Oct4-POUhd /ApEinfo_fwdcolor=#ea5f46 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2685..3074 /label=Oct4_NTD /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6429..7745 /label=mC-Myc /ApEinfo_fwdcolor=#4c4c4c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4878..6299 /label=mKlf-4 /ApEinfo_fwdcolor=#008000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3531..3740 /label=Oct4-CTD /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8846..8874 /label=AmpR_promoter /ApEinfo_fwdcolor=#ff502e /ApEinfo_revcolor=#ff502e /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2619..2637 /label=miniCMV-F-primer /ApEinfo_fwdcolor=#e86b83 /ApEinfo_revcolor=#04fc0d /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2556..2675 /label=CMV2 promoter /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2238..2554 /label=TetO(1) /ApEinfo_label=TetO /ApEinfo_fwdcolor=#6e150c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2619..2637 /label=miniCMV-primer-F /ApEinfo_fwdcolor=#ffa6fe /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4851..6299 /label=Klf4-full /ApEinfo_fwdcolor=#62ff18 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca 2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat 2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg 2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc 2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa 2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg 2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg 2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct 3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc 3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc 3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg 3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca 3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca 3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa 3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc 3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg 3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt 3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc 3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac 3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg 3721 gctctcccat gcattcaaac gctagcggca gcggcgccac Gaacttctct ctgTtaaagc 3781 aagcaggAga tgttgaagaa aaccccgggc ctgcTtgcat gtataacatg atggagacgg 3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca 3901 cggcggcggc gaccggcggc aaccagaaga acagcccgGA CCGCGTCAAG AGGCCCATGA 3961 ACGCCTTCAT GGTATGGTCC CGGGGGCAGC GGCGTAAGAT GGCCCAGGAG AACCCCAAGA 4021 TGCACAACTC GGAGATCAGC AAGCGCCTGG GCGCGGAGTG GAAACTTTTG TCCGAGACCG 4081 AGAAGCGGCC GTTCATCGAC GAGGCCAAGC GGCTGCGCGT GCTGCACATG aaggagcacc 4141 cggattataa ataccggccg cggcggaaaa ccaagacgct catgaagaag gataagtaca 4201 cgcttcccgg aggcttgctg gcccccggcg ggaacagcat ggcgagcggg gttggggtgg 4261 gcgccggcct gggtgcgggc gtgaaccagc gcatggacag ctacgcgcac atgaacggct 4321 ggagcaacgg cagctacagc atgatgcagg agcagctggg ctacccgcag cacccgggcc 4381 tcaacgctca cggcgcggca cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc 4441 agtacaactc catgaccagc tcgcagacct acatgaacgg ctcgcccacc tacagcatgt 4501 cctactcgca gcagggcacc cccggtatgg cgctgggctc catgggctct gtggtcaagt 4561 ccgaggccag ctccagcccc cccgtggtta cctcttcctc ccactccagg gcgccctgcc 4621 aggccgggga cctccgggac atgatcagca tgtacctccc cggcgccgag gtgccggagc 4681 ccgctgcgcc cagtagactg cacatggccc agcactacca gagcggcccg gtgcccggca 4741 cggccattaa cggcacactg cccctgtcgc acatggcatg cggctccggc gagggcaggg 4801 gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgag atgaggcagc 4861 cacctggcga gtctgacatg gctgtcagcg acgctctgct cccgtccttc tccacgttcg 4921 cgtccggccc ggcgggaagg gagaagacac tgcgtccagc aggtgccccg actaaccgtt 4981 ggcgtgagga actctctcac atgaagcgac ttcccccact tcccggccgc ccctacgacc 5041 tggcggcgac ggtggccaca gacctggaga gtggcggagc tggtgcagct tgcagcagta 5101 acaacccggc cctcctagcc cggagggaga ccgaggagtt caacgacctc ctggacctag 5161 actttatcct ttccaactcg ctaacccacc aggaatcggt ggccgccacc gtgaccacct 5221 cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg ccctgccagc gcgccctcca 5281 cctgcagctt cagctatccg atccgggccg ggggtgaccc gggcgtggct gccagcaaca 5341 caggtggagg gctcctctac agccgagaat ctgcgccacc tcccacggcc cccttcaacc 5401 tggcggacat caatgacgtg agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg 5461 agttggaccc agtatacatt ccgccacagc agcctcagcc gccaggtggc gggctgatgg 5521 gcaagtttgt gctgaaggcg tctctgacca cccctggcag cgagtacagc agcccttcgg 5581 tcatcagtgt tagcaaagga agcccagacg gcagccaccc cgtggtagtg gcgccctaca 5641 gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg 5701 tcagccggtc cctagaggcc catttgagcg ctggacccca gctcagcaac ggccaccggc 5761 ccaacacaca cgacttcccc ctggggcggc agctccccac caggactacc cctacactga 5821 gtcccgagga actgctgaac agcagggact gtcaccctgg cctgcctctt cccccaggat 5881 tccatcccca tccggggccc aactaccctc ctttcctgcc agaccagatg cagtcacaag 5941 tcccctctct ccattatcaa gagctcatgc caccgggttc ctgcctgcca gaggagccca 6001 agccaaagag gggaagaagg tcgtggcccc ggaaaagaac agccacccac acttgtgact 6061 atgcaggctg tggcaaaacc tataccaaga gttctcatct caaggcacac ctgcgaactc 6121 acacaggcga gaaaccttac cactgtgact gggacggctg tgggtggaaa ttcgcccgct 6181 ccgatgaact gaccaggcac taccgcaaac acacagggca ccggcccttt cagtgccaga 6241 agtgcgacag ggccttttcc aggtcggacc accttgcctt acacatgaag aggcactttc 6301 tcgagggctc gggccagtgt actaattatg ctctcttgaa attggctgga gatgttgaga 6361 gcaacccagg tcccggcgcg ccactggatt tcctttgggc gttggaaacc ccgcagacag 6421 ccacgacgat gcccctcaac gtgaacttca ccaacaggaa ctatgacctc gactacgact 6481 ccgtacagcc ctatttcatc tgcgacgagg aagagaattt ctatcaccag caacagcaga 6541 gcgagctgca gccgcccgcg cccagtgagg atatctggaa gaaattcgag ctgcttccca 6601 ccccgcccct gtccccgagc cgccgctccg ggctctgctc tccatcctat gttgcggtcg 6661 ctacgtcctt ctccccaagg gaagacgatg acggcggcgg tggcaacttc tccaccgccg 6721 atcagctgga gatgatgacc gagttacttg gaggagacat ggtgaaccag agcttcatct 6781 gcgatcctga cgacgagacc ttcatcaaga acatcatcat ccaggactgt atgtggagcg 6841 gtttctcagc cgctgccaag ctggtctcgg agaagctggc ctcctaccag gctgcgcgca 6901 aagacagcac cagcctgagc cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt 6961 acctgcagga cctcaccgcc gccgcgtccg agtgcattga cccctcagtg gtctttccct 7021 acccgctcaa cgacagcagc tcgcccaaat cctgtacctc gtccgattcc acggccttct 7081 ctccttcctc ggactcgctg ctgtcctccg agtcctcccc acgggccagc cctgagcccc 7141 tagtgctgca tgaggagaca ccgcccacca ccagcagcga ctctgaagaa gagcaagaag 7201 atgaggaaga aattgatgtg gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg 7261 agtcgggctc atctccatcc cgaggccaca gcaaacctcc gcacagccca ctggtcctca 7321 agaggtgcca cgtctccact caccagcaca actacgccgc acccccctcc acaaggaagg 7381 actatccagc tgccaagagg gccaagttgg acagtggcag ggtcctgaag cagatcagca 7441 acaaccgcaa gtgctccagc cccaggtcct cagacacgga ggaaaacgac aagaggcgga 7501 cacacaacgt cttggaacgt cagaggagga acgagctgaa gcgcagcttt tttgccctgc 7561 gtgaccagat ccctgaattg gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa 7621 aagccaccgc ctacatcctg tccattcaag cagacgagca caagctcacc tctgaaaagg 7681 acttattgag gaaacgacga gaacagttga aacacaaact cgaacagctt cgaaactctg 7741 gtgcataaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact 7801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 7861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 7921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 7981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 8041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 8101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 8161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 8221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 8281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 8341 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 8401 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca 8461 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat 8521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 8581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 8641 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc 8701 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 8761 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 8821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 8881 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 8941 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 9001 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 9061 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 9121 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 9181 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 9241 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 9301 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 9361 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 9421 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 9481 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 9541 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 9601 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 9661 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 9721 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 9781 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 9841 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 9901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 9961 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 10021 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 10081 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 10141 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 10201 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 10261 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 10321 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 10381 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 10441 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 10501 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 10561 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 10621 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 10681 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 10741 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat 10801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 10861 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga 10921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 10981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 11041 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc 11101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 11161 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 11221 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 11281 gaaattgtat ttgttaaata tgtactacaa acttagtagt //