LOCUS pHAGE2_TetOminiC 10414 bp ds-DNA circular 16-DEC-2021 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers LTR 1..636 /gene="LTR" /vntifkey="19" /note="Long Terminal Repeat" /locus_tag="5'\LTR" /label="5'\LTR" /ApEinfo_label="5'\LTR" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 637..1155 /gene="psi" /product="pbs-gag" /vntifkey="21" /note="PSI Packaging Sequence" /locus_tag="PSI" /label="PSI" /ApEinfo_label="PSI" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1303..1536 /gene="rre" /product="minimal RRE" /vntifkey="21" /note="Rev Responsive Element" /locus_tag="RRE" /label="RRE" /ApEinfo_label="RRE" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2034..2211 /gene="cppt" /vntifkey="21" /note="Central Poly Purine Track" /locus_tag="HIV\cpPu\(Trip)" /label="HIV\cpPu\(Trip)" /ApEinfo_label="HIV\cpPu\(Trip)" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 6855..7446 /gene="WPRE" /vntifkey="21" /note="Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element" /locus_tag="WPRE" /label="WPRE" /ApEinfo_label="WPRE" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 8028..8867 /gene="AMPr" /product="beta-lactamase (mature form)" /vntifkey="4" /note="Ampicillin Resistance Gene" /locus_tag="AMPr" /label="AMPr" /ApEinfo_label="AMPr" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 9096..9629 /gene="ORI" /product="ColE1 origin of replication" /vntifkey="33" /note="Bacterial Origin of Replication" /locus_tag="ORI" /label="ORI" /ApEinfo_label="ORI" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 9866..9989 /gene="SV40 ori" /vntifkey="21" /locus_tag="SV40\ori" /label="SV40\ori" /ApEinfo_label="SV40\ori" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 10309..10337 /vntifkey="28" /locus_tag="pHAGE-S1-500" /label="pHAGE-S1-500" /ApEinfo_label="pHAGE-S1-500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 401..421 /vntifkey="28" /locus_tag="pHAGE-S501-1000" /label="pHAGE-S501-1000" /ApEinfo_label="pHAGE-S501-1000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 898..919 /vntifkey="28" /locus_tag="pHAGE-S1001-1500" /label="pHAGE-S1001-1500" /ApEinfo_label="pHAGE-S1001-1500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1391..1417 /vntifkey="28" /locus_tag="pHAGE-S1501-2000" /label="pHAGE-S1501-2000" /ApEinfo_label="pHAGE-S1501-2000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1898..1922 /vntifkey="28" /locus_tag="pHAGE-S2001-2500" /label="pHAGE-S2001-2500" /ApEinfo_label="pHAGE-S2001-2500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 7114..7134 /vntifkey="28" /locus_tag="pHAGE-S4001-4500" /label="pHAGE-S4001-4500" /ApEinfo_label="pHAGE-S4001-4500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 7804..7826 /vntifkey="28" /locus_tag="pHAGE-S6001-6500" /label="pHAGE-S6001-6500" /ApEinfo_label="pHAGE-S6001-6500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 8277..8301 /vntifkey="28" /locus_tag="pHAGE-S6501-7000" /label="pHAGE-S6501-7000" /ApEinfo_label="pHAGE-S6501-7000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 8797..8821 /vntifkey="28" /locus_tag="pHAGE-S7001-7500" /label="pHAGE-S7001-7500" /ApEinfo_label="pHAGE-S7001-7500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 9301..9325 /vntifkey="28" /locus_tag="pHAGE-S7501-8000" /label="pHAGE-S7501-8000" /ApEinfo_label="pHAGE-S7501-8000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 9804..9823 /vntifkey="28" /locus_tag="pHAGE-S8001-8500" /label="pHAGE-S8001-8500" /ApEinfo_label="pHAGE-S8001-8500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(158..179) /vntifkey="28" /locus_tag="pHAGE-A0050-8050" /label="pHAGE-A0050-8050" /ApEinfo_label="pHAGE-A0050-8050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(667..690) /vntifkey="28" /locus_tag="pHAGE-A0550-0050" /label="pHAGE-A0550-0050" /ApEinfo_label="pHAGE-A0550-0050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1166..1189) /vntifkey="28" /locus_tag="pHAGE-A1050-0550" /label="pHAGE-A1050-0550" /ApEinfo_label="pHAGE-A1050-0550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1650..1678) /vntifkey="28" /locus_tag="pHAGE-A1550-1050" /label="pHAGE-A1550-1050" /ApEinfo_label="pHAGE-A1550-1050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2084..2112) /vntifkey="28" /locus_tag="pHAGE-A2050-1550" /label="pHAGE-A2050-1550" /ApEinfo_label="pHAGE-A2050-1550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(6961..6984) /vntifkey="28" /locus_tag="pHAGE-A3550-3050" /label="pHAGE-A3550-3050" /ApEinfo_label="pHAGE-A3550-3050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(8077..8100) /vntifkey="28" /locus_tag="pHAGE-A6050-5550" /label="pHAGE-A6050-5550" /ApEinfo_label="pHAGE-A6050-5550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(8546..8569) /vntifkey="28" /locus_tag="pHAGE-A6550-6050" /label="pHAGE-A6550-6050" /ApEinfo_label="pHAGE-A6550-6050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(9057..9078) /vntifkey="28" /locus_tag="pHAGE-A7050-6550" /label="pHAGE-A7050-6550" /ApEinfo_label="pHAGE-A7050-6550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(9568..9589) /vntifkey="28" /locus_tag="pHAGE-A7550-7050" /label="pHAGE-A7550-7050" /ApEinfo_label="pHAGE-A7550-7050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(10044..10070) /vntifkey="28" /locus_tag="pHAGE-A8050-7550" /label="pHAGE-A8050-7550" /ApEinfo_label="pHAGE-A8050-7550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 455..551 /vntifkey="21" /note="HIV Repeat Region" /locus_tag="HIV\R" /label="HIV\R" /ApEinfo_label="HIV\R" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 636..658 /vntifkey="21" /note="tRNA binding site for Lysine tRNA" /locus_tag="PBS-K" /label="PBS-K" /ApEinfo_label="PBS-K" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 697..806 /vntifkey="21" /locus_tag="HIV-PSI" /label="HIV-PSI" /ApEinfo_label="HIV-PSI" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5523..6839 /locus_tag="mC-Myc" /label="mC-Myc" /ApEinfo_label="mC-Myc" /ApEinfo_fwdcolor="#4c4c4c" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 807..1144 /vntifkey="21" /note="HIV gag sequence 351bp to 688bp" /locus_tag="HR-ePSI" /label="HR-ePSI" /ApEinfo_label="HR-ePSI" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 552..636 /vntifkey="21" /note="HIV U5" /locus_tag="HIV\U5" /label="HIV\U5" /ApEinfo_label="HIV\U5" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3972..5393 /locus_tag="mKlf-4" /label="mKlf-4" /ApEinfo_label="mKlf-4" /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1..454 /vntifkey="21" /note="Full Length HIV U3" /locus_tag="HIV\U3" /label="HIV\U3" /ApEinfo_label="HIV\U3" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 7975..8009 /vntifkey="30" /note="Beta Lactamase Promoter" /locus_tag="P-Bla" /label="P-Bla" /ApEinfo_label="P-Bla" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(6883..6917) /vntifkey="28" /locus_tag="pHAGE\3'\CDS" /label="pHAGE\3'\CDS" /ApEinfo_label="pHAGE\3'\CDS" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7451..7552 /vntifkey="21" /note="HIV nef" /locus_tag="HIV\nef" /label="HIV\nef" /ApEinfo_label="HIV\nef" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7593..7689 /vntifkey="21" /locus_tag="HIV\R(1)" /label="HIV\R(1)" /ApEinfo_label="HIV\R" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7690..7772 /vntifkey="21" /note="HIV U5" /locus_tag="HIV\U5(1)" /label="HIV\U5(1)" /ApEinfo_label="HIV\U5" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7501..7515 /vntifkey="21" /note="PolyPurine Tract" /locus_tag="HIV\PPT" /label="HIV\PPT" /ApEinfo_label="HIV\PPT" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" mutation 833..834 /vntifkey="62" /note="Filled in Cla Site from original HIV genome to disrupt gag polyprotein ORF" /locus_tag="Fill-in" /label="Fill-in" /ApEinfo_label="Fill-in" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" LTR 7451..7772 /vntifkey="19" /locus_tag="3'\LTR" /label="3'\LTR" /ApEinfo_label="3'\LTR" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" splicing_signal 744..745 /vntifkey="38" /note="Major 5' Splice Donor 289" /locus_tag="5'\SD\289" /label="5'\SD\289" /ApEinfo_label="5'\SD\289" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" splicing_signal 1911..1912 /vntifkey="38" /locus_tag="SA\7925" /label="SA\7925" /ApEinfo_label="SA\7925" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" splicing_signal 2188..2189 /vntifkey="38" /locus_tag="SA\4459" /label="SA\4459" /ApEinfo_label="SA\4459" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" mutation 1357..1357 /vntifkey="62" /note="This G is a C in the WT HIV-1 genome" /locus_tag="RRE\Mutation" /label="RRE\Mutation" /ApEinfo_label="RRE\Mutation" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2014..2027 /vntifkey="21" /note="Linker Junk Sequence probably left over from cloning" /locus_tag="Junk" /label="Junk" /ApEinfo_label="Junk" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 1156..2013 /vntifkey="4" /note="HIV env sequence from bp7168 to 8025 (contains RRE) Cloned from BglII to BamHI out of HIV" /locus_tag="env" /label="env" /ApEinfo_label="env" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 790..1144 /vntifkey="4" /note="HIV gag sequence from 336bp to 688bp (Clone to PvuII site in HIV and filled in ClaI digest to take gag CDS out of frame)" /locus_tag="HIV\gag" /label="HIV\gag" /ApEinfo_label="HIV\gag" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" 5'UTR 551..635 /vntifkey="52" /note="HIV 5' UTR" /locus_tag="5'\UTR" /label="5'\UTR" /ApEinfo_label="5'\UTR" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 853..855 /vntifkey="21" /note="Stop codon in gag put in frame by ClaI fill in" /locus_tag="Stop\Codon" /label="Stop\Codon" /ApEinfo_label="Stop\Codon" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1245..1593 /vntifkey="21" /note="Rev Responsive Element according to Belasco in Molecular Cell, Vol. 7, 603-614, March, 2001" /locus_tag="RRE(1)" /label="RRE(1)" /ApEinfo_label="RRE" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7518..7554 /vntifkey="21" /note="1st Half of deleted U3" /locus_tag="dU3" /label="dU3" /ApEinfo_label="dU3" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7574..7592 /vntifkey="21" /note="2nd Half of deleted U3" /locus_tag="dU3(1)" /label="dU3(1)" /ApEinfo_label="dU3" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 697..731 /vntifkey="39" /note="PSI Stem loop 1" /locus_tag="SL-1" /label="SL-1" /ApEinfo_label="SL-1" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 736..754 /vntifkey="39" /note="PSI Stem loop 2" /locus_tag="SL-2" /label="SL-2" /ApEinfo_label="SL-2" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 766..779 /vntifkey="39" /note="PSI Stem Loop 3" /locus_tag="SL-3" /label="SL-3" /ApEinfo_label="SL-3" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 793..806 /vntifkey="39" /note="PSI Stem Loop 4" /locus_tag="SL-4" /label="SL-4" /ApEinfo_label="SL-4" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_site 550..550 /vntifkey="26" /note="Exact base to which Poly-A is added" /locus_tag="pA\Site" /label="pA\Site" /ApEinfo_label="pA\Site" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 570..577 /vntifkey="25" /note="GT Cluster shown to be necessary for Poly-A site activity by Cullen in J Virol. 1989 January; 63(1):421-424" /locus_tag="G-T\cluster" /label="G-T\cluster" /ApEinfo_label="G-T\cluster" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2556..2675 /vntifkey="29" /locus_tag="miniCMV" /label="miniCMV" /ApEinfo_label="miniCMV" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2238..2555 /vntifkey="21" /note="Tetracyclin responsive element (TRE)" /locus_tag="TetO" /label="TetO" /ApEinfo_label="TetO" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 8965..9647 /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_binding 10110..10132 /locus_tag="LacO" /label="LacO" /ApEinfo_label="LacO" /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 8208..8867 /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7159..7238 /locus_tag="WPRE(1)" /label="WPRE(1)" /ApEinfo_label="WPRE" /ApEinfo_fwdcolor="#a5c4b3" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2062..2076 /locus_tag="cPPT" /label="cPPT" /ApEinfo_label="cPPT" /ApEinfo_fwdcolor="#a596b4" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7501..7515 /locus_tag="cPPT(1)" /label="cPPT(1)" /ApEinfo_label="cPPT" /ApEinfo_fwdcolor="#a596b4" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2949..2987 /locus_tag="HMG-Sox2" /label="HMG-Sox2" /ApEinfo_label="HMG-Sox2" /ApEinfo_fwdcolor="#400080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2808..2933 /locus_tag="HMG-Sox2(1)" /label="HMG-Sox2(1)" /ApEinfo_label="HMG-Sox2" /ApEinfo_fwdcolor="#400080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2994..3065 /locus_tag="HMG-Sox17" /label="HMG-Sox17" /ApEinfo_label="HMG-Sox17" /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2934..2948 /locus_tag="HMG-Sox17(1)" /label="HMG-Sox17(1)" /ApEinfo_label="HMG-Sox17" /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2988..2993 /locus_tag="HMG-Sox17(2)" /label="HMG-Sox17(2)" /ApEinfo_label="HMG-Sox17" /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca 2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat 2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgatg tataacatga 2701 tggagacgga gctgaagccg ccgggcccgc agcaagcttc ggggggcggc ggcggaggag 2761 gcaacgccac ggcggcggcg accggcggca accagaagaa cagcccgGAC CGCGTCAAGA 2821 GGCCCATGAA CGCCTTCATG GTATGGTCCC GGGGGCAGCG GCGTAAGATG GCCCAGGAGA 2881 ACCCCAAGAT GCACAACTCG GAGATCAGCA AGCGCCTGGG CGCGGAGTGG AAAGCGTTGA 2941 CCTTGGCAGA GAAGCGGCCG TTCATCGACG AGGCCAAGCG GCTGCGCGTG CtGCATATGc 3001 aggaccaccc caactacaag taccggccgc ggcggcgcaa gcaggtgaag cgcatgaagc 3061 gggtggaggg aggcttcctg cacgctctcg tcgagcccca ggccggcgcg cttggtcccg 3121 agggcggccg cgtggccatg gatggcctgg gtctgccttt cccggagccg ggctatccgg 3181 ccggtcctcc gctgatgtct ccgcacatgg gcccccacta tcgggactgc cagggactgg 3241 gcgctcccgc gctcgacggc taccctctgc ccactccgga cacatccccg ctggatggcg 3301 tggagcagga cccggctttc tttgcagccc cgctgccagg ggactgcccg gcggccggca 3361 cctacactta cgctccagtc tcggactatg cagtgtccgt agagccgccc gctggcccca 3421 tgcgagtggg gccggacccc tcgggccctg cgatgccggg gatcctggcg ccccccagcg 3481 ctctgcacct gtactacggc gcgatgggct cgcccgccgc aagtgcgggg cgcggtttcc 3541 acgcgcaacc ccagcagccg ctgcaaccgc aggcaccgcc Cccgccaccg cagcagcagc 3601 acccagcgca cggccccggg caaccttcgc cccctcccga ggctctgccc tgccgggatg 3661 gcacggaatc caaccagccc actgagctcc taggggaggt ggaccgcacg gaatttgaac 3721 agtatctgcc ctttgtgtat aagcccgaga tgggtcttcc ctaccaggga cacgactgcg 3781 gagtgaacct ctcagacagc cacggagcca tttcctccgt ggtgtccgac gctagctcag 3841 cggtctacta ttgcaactac cccgacattg catgcggctc cggcgagggc aggggaagtc 3901 ttctaacatg cggggacgtg gaggaaaatc ccggcccact cgagatgagg cagccacctg 3961 gcgagtctga catggctgtc agcgacgctc tgctcccgtc cttctccacg ttcgcgtccg 4021 gcccggcggg aagggagaag acactgcgtc cagcaggtgc cccgactaac cgttggcgtg 4081 aggaactctc tcacatgaag cgacttcccc cacttcccgg ccgcccctac gacctggcgg 4141 cgacggtggc cacagacctg gagagtggcg gagctggtgc agcttgcagc agtaacaacc 4201 cggccctcct agcccggagg gagaccgagg agttcaacga cctcctggac ctagacttta 4261 tcctttccaa ctcgctaacc caccaggaat cggtggccgc caccgtgacc acctcggcgt 4321 cagcttcatc ctcgtcttcc ccagcgagca gcggccctgc cagcgcgccc tccacctgca 4381 gcttcagcta tccgatccgg gccgggggtg acccgggcgt ggctgccagc aacacaggtg 4441 gagggctcct ctacagccga gaatctgcgc cacctcccac ggcccccttc aacctggcgg 4501 acatcaatga cgtgagcccc tcgggcggct tcgtggctga gctcctgcgg ccggagttgg 4561 acccagtata cattccgcca cagcagcctc agccgccagg tggcgggctg atgggcaagt 4621 ttgtgctgaa ggcgtctctg accacccctg gcagcgagta cagcagccct tcggtcatca 4681 gtgttagcaa aggaagccca gacggcagcc accccgtggt agtggcgccc tacagcggtg 4741 gcccgccgcg catgtgcccc aagattaagc aagaggcggt cccgtcctgc acggtcagcc 4801 ggtccctaga ggcccatttg agcgctggac cccagctcag caacggccac cggcccaaca 4861 cacacgactt ccccctgggg cggcagctcc ccaccaggac tacccctaca ctgagtcccg 4921 aggaactgct gaacagcagg gactgtcacc ctggcctgcc tcttccccca ggattccatc 4981 cccatccggg gcccaactac cctcctttcc tgccagacca gatgcagtca caagtcccct 5041 ctctccatta tcaagagctc atgccaccgg gttcctgcct gccagaggag cccaagccaa 5101 agaggggaag aaggtcgtgg ccccggaaaa gaacagccac ccacacttgt gactatgcag 5161 gctgtggcaa aacctatacc aagagttctc atctcaaggc acacctgcga actcacacag 5221 gcgagaaacc ttaccactgt gactgggacg gctgtgggtg gaaattcgcc cgctccgatg 5281 aactgaccag gcactaccgc aaacacacag ggcaccggcc ctttcagtgc cagaagtgcg 5341 acagggcctt ttccaggtcg gaccaccttg ccttacacat gaagaggcac tttctcgagg 5401 gctcgggcca gtgtactaat tatgctctct tgaaattggc tggagatgtt gagagcaacc 5461 caggtcccgg cgcgccactg gatttccttt gggcgttgga aaccccgcag acagccacga 5521 cgatgcccct caacgtgaac ttcaccaaca ggaactatga cctcgactac gactccgtac 5581 agccctattt catctgcgac gaggaagaga atttctatca ccagcaacag cagagcgagc 5641 tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt cgagctgctt cccaccccgc 5701 ccctgtcccc gagccgccgc tccgggctct gctctccatc ctatgttgcg gtcgctacgt 5761 ccttctcccc aagggaagac gatgacggcg gcggtggcaa cttctccacc gccgatcagc 5821 tggagatgat gaccgagtta cttggaggag acatggtgaa ccagagcttc atctgcgatc 5881 ctgacgacga gaccttcatc aagaacatca tcatccagga ctgtatgtgg agcggtttct 5941 cagccgctgc caagctggtc tcggagaagc tggcctccta ccaggctgcg cgcaaagaca 6001 gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc cacctccagc ctgtacctgc 6061 aggacctcac cgccgccgcg tccgagtgca ttgacccctc agtggtcttt ccctacccgc 6121 tcaacgacag cagctcgccc aaatcctgta cctcgtccga ttccacggcc ttctctcctt 6181 cctcggactc gctgctgtcc tccgagtcct ccccacgggc cagccctgag cccctagtgc 6241 tgcatgagga gacaccgccc accaccagca gcgactctga agaagagcaa gaagatgagg 6301 aagaaattga tgtggtgtct gtggagaaga ggcaaacccc tgccaagagg tcggagtcgg 6361 gctcatctcc atcccgaggc cacagcaaac ctccgcacag cccactggtc ctcaagaggt 6421 gccacgtctc cactcaccag cacaactacg ccgcaccccc ctccacaagg aaggactatc 6481 cagctgccaa gagggccaag ttggacagtg gcagggtcct gaagcagatc agcaacaacc 6541 gcaagtgctc cagccccagg tcctcagaca cggaggaaaa cgacaagagg cggacacaca 6601 acgtcttgga acgtcagagg aggaacgagc tgaagcgcag cttttttgcc ctgcgtgacc 6661 agatccctga attggaaaac aacgaaaagg cccccaaggt agtgatcctc aaaaaagcca 6721 ccgcctacat cctgtccatt caagcagacg agcacaagct cacctctgaa aaggacttat 6781 tgaggaaacg acgagaacag ttgaaacaca aactcgaaca gcttcgaaac tctggtgcat 6841 aaatcgatAG ATCCtaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 6901 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 6961 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 7021 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 7081 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 7141 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 7201 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 7261 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 7321 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 7381 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 7441 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 7501 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atcacctgca 7561 ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 7621 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 7681 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 7741 acccttttag tcagtgtgga aaatctctag cacccgggcg attaaggaaa gggctagatc 7801 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 7861 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 7921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 8041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 8101 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 8161 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 8221 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 8281 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 8341 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 8401 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 8461 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 8521 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 8581 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 8641 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 8701 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 8761 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 8821 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 8881 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8941 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 9001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 9061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 9121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 9181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 9241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 9301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 9361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 9421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 9481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 9541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 9601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 9661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 9721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 9781 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 9841 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc 9901 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 9961 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg 10021 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 10081 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 10141 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta 10201 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc 10261 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc 10321 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt 10381 gtatttgtta aatatgtact acaaacttag tagt //