LOCUS       Exported               10257 bp ds-DNA     circular SYN 26-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..675
                     /label=NC Frame
     terminator      707..959
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    981..1005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2305..2934
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3368..4435
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4501..4695
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(5365..5953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6040..6834)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7259..7283
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7361..7535
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7592..8389)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(8452..9129)
                     /label=CaMV 35S promoter
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        9356..9386
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9394..9410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9430..9663
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     promoter        9897..10242
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agaccgtgtg aattggtgac cagctcgaat ttccccgatc gttcaaacat
      721 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
      781 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
      841 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
      901 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
      961 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     1021 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     1081 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     1141 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     1201 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     1261 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     1321 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     1381 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     1441 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     1501 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     1561 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     1621 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     1681 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggcgc
     1741 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1801 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1861 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     1921 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     1981 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     2041 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     2101 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     2161 ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     2221 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     2281 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     2341 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     2401 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     2461 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     2521 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     2581 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     2641 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     2701 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2761 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2821 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     2881 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     2941 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     3001 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     3061 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     3121 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     3181 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     3241 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     3301 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     3361 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     3421 ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     3481 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     3541 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     3601 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     3661 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     3721 cgggcacgta gaggtttccg cagggccggc gggcatggcc agtgtgtggg attacgacct
     3781 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     3841 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     3901 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     3961 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     4021 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     4081 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     4141 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     4201 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     4261 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     4321 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     4381 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     4441 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     4501 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     4561 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     4621 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     4681 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     4741 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     4801 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     4861 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     4921 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4981 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     5041 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     5101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5161 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5221 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5281 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5941 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6001 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     6061 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     6121 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     6181 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     6241 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     6301 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     6361 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     6421 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     6481 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     6541 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     6601 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     6661 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     6721 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     6781 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     6841 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     6901 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     6961 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     7021 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     7081 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     7141 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     7201 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     7261 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     7321 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
     7381 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     7441 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     7501 tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat cgactctagc
     7561 tagaggatcg atccgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     7621 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     7681 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     7741 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     7801 tattcggcaa gcaggcatcg ccatgtgtca cgacgagatc ctcgccgtcg ggcatgcgcg
     7861 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     7921 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     7981 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     8041 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     8101 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     8161 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctggagt tcattcaggg
     8221 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     8281 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     8341 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatccccatg gtcgatcgac
     8401 agatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
     8461 cctctccaaa tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat
     8521 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
     8581 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     8641 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
     8701 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
     8761 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
     8821 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca
     8881 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     8941 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     9001 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     9061 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     9121 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     9181 agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc
     9241 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9421 gaaacagcta tgaccatgat tacgaattcg agctcggtac ccggggatcc tctagagtcg
     9481 acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9541 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9601 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc
     9661 tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttag cttcatggag
     9721 tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata
     9781 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
     9841 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     9901 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     9961 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
    10021 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
    10081 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    10141 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    10201 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac tcttgac
//
