LOCUS       Exported               12788 bp ds-DNA     circular SYN 26-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..675
                     /label=NC Frame
     promoter        1086..1431
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1463..2173
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2180..3985
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      4041..4293
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4315..4339
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5639..6268
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6702..7769
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7835..8029
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(8699..9287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9374..10168)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10593..10617
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10695..10869
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10926..11723)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(11786..12463)
                     /label=CaMV 35S promoter
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        12690..12720
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12728..12744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agaccaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
      721 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      781 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      841 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttagc
      901 ttcatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
      961 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     1021 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
     1081 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     1141 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     1201 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     1261 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1321 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     1381 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact
     1441 cttgaccatg gtagatctga ctagtaaagg agaagaactt ttcactggag ttgtcccaat
     1501 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1561 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1621 tgttccgtgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcaagata
     1681 cccagatcat atgaagcggc acgacttctt caagagcgcc atgcctgagg gatacgtgca
     1741 ggagaggacc atcttcttca aggacgacgg gaactacaag acacgtgctg aagtcaagtt
     1801 tgagggagac accctcgtca acaggatcga gcttaaggga atcgatttca aggaggacgg
     1861 aaacatcctc ggccacaagt tggaatacaa ctacaactcc cacaacgtat acatcatggc
     1921 cgacaagcaa aagaacggca tcaaagccaa cttcaagacc cgccacaaca tcgaagacgg
     1981 cggcgtgcaa ctcgctgatc attatcaaca aaatactcca attggcgatg gccctgtcct
     2041 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     2101 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2161 tgaactatac aaagctagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
     2221 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
     2281 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
     2341 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
     2401 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
     2461 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
     2521 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
     2581 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
     2641 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
     2701 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
     2761 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
     2821 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
     2881 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
     2941 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
     3001 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
     3061 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
     3121 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
     3181 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
     3241 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
     3301 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
     3361 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
     3421 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
     3481 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     3541 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     3601 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     3661 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     3721 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     3781 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     3841 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     3901 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     3961 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg
     4021 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     4081 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     4141 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     4201 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     4261 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag
     4321 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
     4381 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     4441 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     4501 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     4561 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     4621 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     4681 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     4741 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     4801 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     4861 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     4921 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     4981 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     5041 cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg tgaagtttgg cccccgccct
     5101 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     5161 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     5221 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     5281 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     5341 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     5401 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     5461 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggcgagc ttggccgctg
     5521 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     5581 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     5641 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     5701 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     5761 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     5821 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     5881 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     5941 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     6001 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     6061 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     6121 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     6181 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     6241 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     6301 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     6361 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     6421 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     6481 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     6541 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     6601 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
     6661 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc
     6721 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg
     6781 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     6841 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     6901 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     6961 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     7021 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     7081 cggcgggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     7141 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     7201 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     7261 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     7321 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     7381 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     7441 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     7501 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     7561 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     7621 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     7681 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     7741 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     7801 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     7861 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     7921 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     7981 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     8041 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     8101 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     8161 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     8221 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     8401 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8461 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     8521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9121 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9361 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     9421 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     9481 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     9541 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     9601 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     9661 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     9721 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     9781 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     9841 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     9901 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     9961 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
    10021 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    10081 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    10141 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    10201 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    10261 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    10321 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
    10381 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
    10441 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
    10501 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
    10561 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
    10621 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
    10681 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
    10741 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
    10801 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
    10861 atggagaaac tcgagcttgt cgatcgactc tagctagagg atcgatccga accccagagt
    10921 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
    10981 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
    11041 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
    11101 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgt
    11161 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
    11221 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
    11281 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
    11341 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
    11401 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
    11461 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
    11521 agccgcgctg cctcgtcctg gagttcattc agggcaccgg acaggtcggt cttgacaaaa
    11581 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
    11641 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
    11701 aatccatctt gttcaatccc catggtcgat cgacagatct gcgaaagctc gagagagata
    11761 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
    11821 atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    11881 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    11941 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    12001 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    12061 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    12121 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
    12181 gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt
    12241 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    12301 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
    12361 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
    12421 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
    12481 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc
    12541 aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    12601 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    12661 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    12721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa
    12781 ttcgagct
//
