LOCUS       Exported               13598 bp ds-DNA     circular SYN 09-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..1824
                     /organism="Binary vector pBI121"
                     /mol_type="genomic DNA"
                     /note="constructed using pB221 from Clontech Laboratories 
                     and Bin19 described in GenBank Accession Number U09365"
                     /db_xref="taxon:189807"
     terminator      9..261
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    278..952
                     /label=NC Frame
     promoter        complement(968..1313)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1828..1844)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2221..2566
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2598..3308
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             3315..5120
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      5176..5428
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5450..5474
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6774..7403
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7837..8904
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8970..9164
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(9834..10422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10509..11303)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11728..11752
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11830..12004
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12011..12562)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12607..13284)
                     /label=CaMV 35S promoter
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     13573..13589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cgagctcggt ctcagcagac cacaagtggc
      301 cagactggcc ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
      361 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
      421 taccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
      481 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
      541 caccatccgt cgcccaggcg tgtcaataat atcactctgt acatccacaa acagacgata
      601 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt
      661 cagcaaaagt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
      721 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
      781 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
      841 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
      901 tgccaactta gtcgagaatg gccgacgagg ccaggactgg acagagacca ctctctagag
      961 tcccccgtgt tctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     1021 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     1081 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     1141 ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat
     1201 ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg
     1261 ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc
     1321 tttggtcttc tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca
     1381 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa gagactctgt atgaactgtt
     1441 cgccagtctt tacggcgagt tctgttaggt cctctatttg aatctttgac tccatggcct
     1501 ttgattcagt gggaactacc tttttagaga ctccaatctc tattacttgc cttggtttgt
     1561 gaagcaagcc ttgaatcgtc catactggaa tagtacttct gatcttgaga aatatatctt
     1621 tctctgtgtt cttgatgcag ttagtcctga atcttttgac tgcatcttta accttcttgg
     1681 gaaggtattt gatttcctgg agattattgc tcgggtagat cgtcttgatg agacctgctg
     1741 cgtaagcctc tctaaccatc tgtgggttag cattctttct gaaattgaaa aggctaatct
     1801 ggggacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     1861 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1921 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1981 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat
     2041 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     2101 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     2161 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     2221 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     2281 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     2341 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     2401 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     2461 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     2521 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga
     2581 ccatggtaga tctgactagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2641 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2701 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2761 cgtggccaac acttgtcact actttctctt atggtgttca atgcttttca agatacccag
     2821 atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga
     2881 ggaccatctt cttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg
     2941 gagacaccct cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca
     3001 tcctcggcca caagttggaa tacaactaca actcccacaa cgtatacatc atggccgaca
     3061 agcaaaagaa cggcatcaaa gccaacttca agacccgcca caacatcgaa gacggcggcg
     3121 tgcaactcgc tgatcattat caacaaaata ctccaattgg cgatggccct gtccttttac
     3181 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     3241 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     3301 tatacaaagc tagtttacgt cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg
     3361 gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt tggtgggaaa
     3421 gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat cagttcgccg
     3481 atgcagatat tcgtaattat gcgggcaacg tctggtatca gcgcgaagtc tttataccga
     3541 aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag
     3601 tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca tttgaagccg
     3661 atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt gtgaacaacg
     3721 aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa
     3781 agcagtctta cttccatgat ttctttaact atgccggaat ccatcgcagc gtaatgctct
     3841 acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc gcgcaagact
     3901 gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc gttgaactgc
     3961 gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac tagcgggact ttgcaagtgg
     4021 tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc gtcacagcca
     4081 aaagccagac agagtgtgat atctacccgc ttcgcgtcgg catccggtca gtggcagtga
     4141 agggccaaca gttcctgatt aaccacaaac cgttctactt tactggcttt ggtcgtcatg
     4201 aagatgcgga cttacgtggc aaaggattcg ataacgtgct gatggtgcac gaccacgcat
     4261 taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac gctgaagaga
     4321 tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct gctgtcggct
     4381 ttcagctgtc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg
     4441 aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa gagctgatag
     4501 cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa ccggataccc
     4561 gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt aaactcgacc
     4621 cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc gataccatca
     4681 gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc caaagcggcg
     4741 atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag gagaaactgc
     4801 atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg cactcaatgt
     4861 acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat caccgcgtct
     4921 ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat tttgcgacct
     4981 cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat cttcactcgc gaccgcaaac
     5041 cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc ggtgaaaaac
     5101 cgcagcaggg aggcaaacaa gctagccacc accaccacca ccacgtgtga attggtgacc
     5161 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     5221 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     5281 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     5341 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     5401 cgcgcggtgt catctatgtt actagatcgg gaattaaact atcagtgttt gacaggatat
     5461 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc
     5521 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     5581 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     5641 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     5701 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     5761 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     5821 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     5881 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     5941 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     6001 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     6061 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     6121 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     6181 ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc gccctaccct
     6241 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     6301 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     6361 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     6421 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     6481 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     6541 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     6601 ctggccggtt tgtctgatgc caagctggcg gcctggccgg cgagcttggc cgctgaagaa
     6661 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     6721 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     6781 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     6841 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     6901 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     6961 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     7021 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     7081 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     7141 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     7201 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     7261 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     7321 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     7381 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     7441 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     7501 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     7561 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     7621 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     7681 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     7741 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     7801 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     7861 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggcggc gcaggccgcc
     7921 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     7981 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     8041 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     8101 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     8161 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcg
     8221 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     8281 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     8341 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     8401 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     8461 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     8521 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     8581 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     8641 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     8701 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     8761 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     8821 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     8881 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     8941 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     9001 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     9061 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     9121 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     9181 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     9241 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     9301 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     9361 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     9421 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9481 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     9541 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9601 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     9661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10021 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10261 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    10501 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
    10561 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
    10621 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
    10681 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
    10741 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
    10801 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
    10861 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
    10921 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
    10981 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
    11041 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
    11101 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
    11161 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
    11221 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
    11281 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
    11341 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    11401 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    11461 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    11521 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    11581 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    11641 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    11701 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    11761 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
    11821 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
    11881 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
    11941 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
    12001 gaaactcgag tcaaatctcg gtgacgggca ggaccggacg gggcggtacc ggcaggctga
    12061 agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca
    12121 gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca
    12181 gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcaggtggg
    12241 tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg ggagacgtac acggtcgact
    12301 cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga
    12361 cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt
    12421 ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt
    12481 agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg
    12541 ccgggcgtcg ttctgggctc atggtagact cgagagagat agatttgtag agagagactg
    12601 gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc
    12661 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
    12721 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
    12781 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
    12841 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
    12901 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
    12961 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
    13021 tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
    13081 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
    13141 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
    13201 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
    13261 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
    13321 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
    13381 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    13441 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    13501 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    13561 aacaatttca cacaggaaac agctatgacc atgattac
//
