LOCUS       Exported                3963 bp ds-DNA     circular SYN 12-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..675
                     /label=NC Frame
     protein_bind    806..822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    1136..1160
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1251..1839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2010..2825)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3116..3551
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3686..3708
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agacccctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
      721 ggtggagctc cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat
      781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      841 ccggaaghca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgaa ggccttgaca
     1141 ggatatattg gcgggtaaac taagtcgctg tatgtgtttg tttgagatct catgtgagca
     1201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1681 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaga
     1741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1981 agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat caaatgaaac
     2041 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     2101 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2161 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2221 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2281 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2341 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2401 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2461 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct
     2521 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2581 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacaacattg
     2641 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     2701 cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2761 tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg
     2821 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     2881 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
     2941 ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac
     3001 cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc
     3061 atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc
     3121 caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt
     3181 atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct
     3241 ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg
     3301 tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat
     3361 agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt
     3421 agtatgttca attagctttt caattatgaa tatatatatc aattattggt cgcccttggc
     3481 ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca
     3541 ccgtcgagcg ccagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat
     3601 aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat
     3661 ttccgatccc cggaattaga gatcttggca ggatatattg tggtgtaacg ttaacattaa
     3721 cgtttacaat ttcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     3781 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     3841 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     3901 aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg
     3961 ata
//
